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Praj Industries BIO TRACK: Company & Technical Presentations Praj Industries Rishi Jain Principal Technologist July 19, 2018 Corporate Overview • Established in 1983 by first generation Technopreneur • TEMP-C1 offerings for process industry • Sustainable & Innovative solutions for energy and environment • Global Leadership in Bio-industrial sector - Ethanol and Renewable chemicals • A unique technology + engineering company, with Global leadership in ethanol • World Class Biotech R&D center - Praj Matrix • Listed on NSE & BSE in India • Responsible Corporate citizen- Environment, Health and Education as core CSR areas TEMP-C1 – Technology, Engineering, Manufacturing, Procurement, Construction Business offerings Bioenergy High Purity Engineering Businesses R&D Critical Customized EPC/ High purity process Research Services Solution applications for Effluent equipment & & Solutions Provider pharma, Beverage Recycling & systems, in Beverage biotech, plants ZLD cosmetics Modular Bio-Product / Ethanol industry Process Skids plants Division Praj – Strong Credentials across the globe EUROPE SOUTH EAST ASIA INDIA AMERICAS AFRICA Global presence with over 750 reference in more than 75 countries Praj Matrix R&D . In-House R&D laboratory - Certification by DSIR*, GOI . 90+ technologists and growing . ~$ 30 mln investment . Number of Patents – 17 . Received status of Private Sector Bio-Tech Park by the Government of Maharashtra, India High throughput Screening M/C *DSIR: Department of Scientific and Industrial Research Matrix : Centers of Excellence Molecular & Bio-Process Microbiology Technology Biologists Chemists Scale-up & Chemical Engineers Process Sciences Engineering Analytical Sciences Praj Matrix employs centers of excellence(COE) model 6 Thanks ! 2nd Gen Bio-refinery (1 MLPA) demonstration plant for ethanol & bio-chemicals Yeast engineering Industrial Feedstocks for ethanol YPD medium Corn grain Cane Molasses 9 Praj’s yeast strains • Indian Sugar Industry in the 1980’s • Sugar cane molasses problem • Praj developed molasses fermentation technology for the production of ethanol • Screened yeast strains • Developed XP and GR yeast strains that are globally used for the production of ethanol • Commercial process • 30 – 100 kilo liters of ethanol per day • > 100 m3 sized fermenters • Fermentation developments • Batch mode • Continuous mode • Fed batch mode Market Leaders in Ethanol by fermentation Yeast Development at Praj Matrix Bioprospecting Yeast Breeding Superior diploid parent Inferior diploid parent Reverse Engineering sporulation sporulation Superior hybrid diploid yeast sporulation screening screening Superior haploid Inferior haploid Crossing x Haploid segregants Superior hybrid diploid yeast screening Molecular Yeast Typing Metabolic Engineering Superior haploids Pooled whole genome sequencing Bioinformatic analysis for identification of Single Nucleotide Polymorphisms (SNP) Engineering of SNP in industrial yeast strain Expertise in working with industrial yeast strains • Yeast breeding • Yeast molecular biology • Sporulation • Marked gene modifications • Tetrad dissection • Scarred gene modifications • Mating • CRISPR/Cas9 based modifications • Yeast characterization • Bioinformatics • Molecular typing • Variant calling • Mating type determination • SNP analysis (homologous or heterologous) • Ploidy determination • Genome scale metabolic modeling • Yeast storage and maintenance • Shake flask fermentation • Bench scale fermentation • Batch mode • Fed-batch mode • Continuous mode • Pilot scale fermentation Ethanol fermentation improvement 25% reduction in fermentation time 23% reduction in glycerol Flavors and Fragrance – Terpenoid pathway Cadinene Humulene Sample Sample Library Library Partnership models Thank you .
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