Brunneocarpos Banksiae Fungal Planet Description Sheets 451

450 Persoonia – Volume 36, 2016

Brunneocarpos banksiae Fungal Planet description sheets 451

Fungal Planet 466 – 4 July 2016 Brunneocarpos Giraldo & Crous, gen. nov.

Etymology. Brunneus (Latin) = brown, and carpos (Greek) = fruit. Ascospores uniseriate, ellipsoidal to slightly fusiform, clavate, Classiﬁcation — Mycocaliciaceae, Mycocaliciales, Eurotio 1-celled, brown, smooth-walled. Mycelium consisting of sep- mycetes. tate, hyaline, smooth- and thin-walled hyphae. Asexual morph producing dictyochlamydospores in chains, subhyaline, thick- Ascomata apothecial, stipitate, growing intermingled among the walled, lateral or terminal. floral bracts on Banksia cones. Stipe straight or flexuous, mostly branched. Capitulum brown, globose to subglobose. Para Type species. Brunneocarpos banksiae Giraldo & Crous. MycoBank MB816972. physes not observed. Asci 8-spored, cylindrical, unitunicate.

Brunneocarpos banksiae Giraldo & Crous, sp. nov. Etymology. Name reflects the host genus Banksia, from which the species Notes — According to LSU and ITS analyses Brunneocar was isolated. pos banksiae belongs to Mycocaliciaceae (Mycocaliciales) Ascomata apothecial, stipitate, growing intermingled among where four genera are currently accepted; Chaenothecopsis, the floral bracts on Banksia attenuata cones. Stipe dark brown, Phaeocalicium, Mycocalicium and Stenocybe. All produce apo- shiny, flexuous, mostly branched two or three times at the apex, thecioid ascomata, usually with a tiny stalk and brown as- 1–2 mm long, 50–85 µm wide. Capitulum dark brown, globose cospores (Tuovila et al. 2011). These genera harbour resi- to subglobose with a funnel-shaped base, covered by hyaline niculous species, growing directly on exudate and/or on exu mycelium when older, 150–285 × 125–206 µm. Paraphyses date-impregnated wood of different hosts, including Acer (Sapin- not observed. Asci 8-spored, cylindrical, unitunicate, 30–34 × daceae), Mangifera (Anacardiaceae), Khaya (Meliaceae), Abies 4–5 µm. Ascospores uniseriate, ellipsoidal to slightly fusiform, (Pinaceae), Picea, Tsuga and Tilia (Malvaceae) (Rikkinen 2003, clavate, 1-celled, brown, thick- and smooth-walled, 4.5–7 × Tuovila et al. 2011) and known species are lignicolous, algicol- 2–3.5 µm. Mycelium consisting of septate, hyaline, smooth- and ous or lichenicolous (Tuovila et al. 2014). So far no species thin-walled hyphae, 1.5–2 µm diam. Conidiophores absent or from these genera have been reported growing on Banksia, poorly differentiated. Dictyochlamydospores in chains, sessile Species growing in axenic culture produce a phialophora-like or with short subconical stalk, subhyaline to pale brown, thick- (Chaenothecopsis shefflerae) or coelomycetous asexual morph and smooth-walled, lateral or terminal, 26–60 µm long. (Tibell 1991, Tibell & Vinuesa 2005), and in some cases, a Culture characteristics — Colonies on MEA reaching 3–3.3 hyphomycetous asexual morph with ramoconidia in acropetal cm diam after 2 mo at 25 °C, vinaceous buff (Rayner 1970), chains (C. haematopus) (Tibell & Constantinescu 1991). This depressed at centre, floccose. morphological character differs from the new taxon proposed here, which produces a chlamydospore-like asexual morph Typus. Australia, Western Australia, S34°22'19.4" E118°1'33.6", on in culture. Banksia attenuata (Proteaceae), 23 Sept. 2015, P.W. Crous (holotype CBS H-22633, cultures ex-type CPC 29841 = CBS 141465, ITS sequence GenBank KX262670, LSU sequence GenBank KX228352.1, MycoBank MB816973); CPC 29070, CPC 29072, CPC 29435.

Colour illustrations. Australia, Western Australia, cones of Banksia at tenuata (photo: P.W. Crous); stipitated apothecium, detail of the capitulum, asci, ascospores, dictyochlamydospore. Scale bar = 10 µm.

Alejandra Giraldo & Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected] Michael J. Wingﬁeld, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa; e-mail: [email protected] Treena I. Burgess & Giles E.St.J. Hardy, Centre for Phytophthora Science and Management, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia; e-mail: [email protected] & [email protected]