DOCSLIB.ORG

Increased Levels of Sphingosine-1-Phosphate In

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Increased Levels of Sphingosine-1-Phosphate In Kułakowska et al. Journal of Neuroinflammation 2014, 11:193 JOURNAL OF http://www.jneuroinflammation.com/content/11/1/193 NEUROINFLAMMATION RESEARCH Open Access Increased levels of sphingosine-1-phosphate in cerebrospinal fluid of patients diagnosed with tick-borne encephalitis Alina Kułakowska1, Fitzroy J Byfield2,Małgorzata Żendzian-Piotrowska3, Joanna M Zajkowska4, Wiesław Drozdowski1, Barbara Mroczko5, Paul A Janmey2 and Robert Bucki2,6,7* Abstract Background: Tick-borne encephalitis (TBE) is a serious acute central nervous system infection that can result in death or long-term neurological dysfunctions. We hypothesize that changes in sphingosine-1-phosphate (S1P) concentration occur during TBE development. Methods: S1P and interleukin-6 (IL-6) concentrations in blood plasma and cerebrospinal fluid (CSF) were measured using HPLC and ELISA, respectively. The effects of S1P on cytoskeletal structure and IL-6 production were assessed using rat astrocyte primary cultures with and without addition of plasma gelsolin and the S1P receptor antagonist fingolimod phosphate (FTY720P). Results: We report that acute inflammation due to TBE virus infection is associated with elevated levels of S1P and IL-6 in the CSF of infected patients. This elevated concentration is observed even at the earliest neurologic stage of disease, and may be controlled by glucocorticosteroid anti-inflammatory treatment, administered to patients unresponsive to antipyretic drugs and who suffer from a fever above 39°C. In vitro, treatment of confluent rat astrocyte monolayers with a high concentration of S1P (5 μM) results in cytoskeletal actin remodeling that can be prevented by the addition of recombinant plasma gelsolin, FTY720P, or their combination. Additionally, gelsolin and FTY720P significantly decreased S1P-induced release of IL-6. Conclusions: TBE is associated with increased concentration of S1P and IL-6 in CSF, and this increase might promote development of inflammation. The consequences of increased extracellular S1P can be modulated by gelsolin and FTY720P. Therefore, blocking the inflammatory response at sites of infection by agents modulating S1P pathways might aid in developing new strategies for TBE treatment. Keywords: Cerebrospinal fluid, Tick-borne encephalitis, Sphingosine-1-phosphate, IL-6, Gelsolin, FTY720P Background has multiple physiological effects [2]. In the immune Sphingosine-1-phosphate (S1P) is a product of sphingo- system, cell surface S1P(1) receptors transduce the myelin (SM) metabolism. It is present in most eukaryotic rapid, transient effects of extracellular S1P on T- and organisms. S1P regulates cell function both intracellu- B-lymphocyte trafficking, promote early T-cell migration larly and by binding to extracellular receptors [1]. As to tissue sites of immune responses, and regulate T-cell an extracellular mediator, S1P binds to a family of proliferation and secretion of numerous cytokines [1,3]. G-protein-coupled receptors named S1P(1)-S1P(5) and S1P receptors are also found on all cell types in the CNS, and the effects of S1P on neurons [4], astrocytes [5,6], oli- * Correspondence: [email protected] godendrocytes [7], and microglia [8,9] are highly cell-type 2Institute for Medicine and Engineering, University of Pennsylvania, 1010 specific. For example, S1P is a chemoattractant for neural Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA precursor cells and is proposed to direct migration of 6Department of Physiology, Pathophysiology and Microbiology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, neurons to sites of injury [10]. Additionally, S1P [7] or Kielce, Al. IX Wieków Kielc 19, Kielce 25-317, Poland Full list of author information is available at the end of the article © 2014 Kułakowska et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Kułakowska et al. Journal of Neuroinflammation 2014, 11:193 Page 2 of 9 http://www.jneuroinflammation.com/content/11/1/193 synthetic ligands of S1P receptors [11,12] interact with heparinized syringes and centrifuged at 1,500 g for 5 mi- neurotrophin-3 to promote survival of oligodendrocytes. nutes at 4°C. The protocol for this study was approved by S1P is abundant in plasma where it is bound to high- the Ethics Committee for Research on Humans, Medical density lipoproteins and albumin [13]. Recently we have University of Białystok (approval number: R-I-002/382/ shown that plasma gelsolin, an actin-binding PIP2-regulated 2012). At the time of patient recruitment, written consent protein, can also act as a universal carrier or scavenger of was obtained from all subjects. All individuals were under- S1P, and its function may include interference with S1P going lumbar puncture for diagnostic purposes, and most actions [14]. Such an interaction may be of importance in of the TBE patients received a second lumbar puncture settings where the concentrations of both substances 10 to 12 days later to monitor the course of the disease. change over their homeostatic ranges. Multiple sclerosis Following lumbar punctures, CSF cells were counted, (MS), a chronic immune-mediated inflammatory disease and the rest of the material (after centrifugation: 2,000 g of the CNS, seems to be one example of such a condition for 10 minutes), along with plasma samples, were frozen [15]. In the CSF of patients with MS, S1P and gelso- and kept at −80°C. lin concentrations showed a tendency to increase and Clinical and laboratory characteristics of the patient decrease, respectively, when compared to other non- groups are given in Table 1. Briefly, all TBE patients inflammatory neurological disorders [15]. An inflamma- were divided into the following groups: without and tory reaction accompanied by central nervous system with glucocorticosteroids (GCs) treatment (+G), from (CNS) infections such as tick-borne encephalitis (TBE) or whom the CSF and plasma were collected at admission Lyme neuroborreliosis (LNB), also results in blood and (TBE-I/TBE + G-I; patients at time of admission and cerebrospinal fluid (CSF) alterations in plasma gelsolin first lumbar puncture without or with GCs treatment [15]. TBE, a systemic infection with RNA virus leads to respectively) and patients from whom the CSF and the development of an acute meningitis and encephalitis, plasma samples were collected after 10 to 12 days of characterized by swelling of the brain due to inflammation treatment (TBE-II/TBE + G-II; patients after 10 to 12 days [16]. Even though TBE can be prevented by active of hospitalization, non-treated or treated with GCs, re- immunization, it is still very common in some regions spectively). Diagnosis of TBE was confirmed by detection of the world, such as Central Europe, and currently no of anti-TBE virus antibodies in the serum and CSF by specific treatment is known [17,18]. We hypothesize that ELISA (Virion-SERION kit, SERION ELISA classic TBE TBE may result in an alteration of S1P concentration in Virus IgG/IgM,Würzburg, Germany). All TBE patients the blood and CSF of patients, and in such a case modula- were given symptomatic treatment (analgesics, antipy- tion of S1P cellular effects may be used to develop new retics, and intravenous rehydration). Additionally, patients treatment strategies. FTY720P, a S1P receptor modulator, in poor general condition (fever above 39°C and seizures was found to be highly effective in the treatment of MS or disturbances of consciousness (Glasgow Coma Scale [19], and its immunomodulatory activity may be poten- score <12 points)) were treated with glucocorticosteroid tially beneficial in other CNS inflammatory conditions. (dexamethasone 12 to 16 mg daily), which was given intravenously during a period of four to six days. Materials and methods LNB was diagnosed according to the European Feder- Materials ation of Neurological Societies criteria [21]. In all our LNB S1P (S9666) was purchased from Sigma Aldrich (St Louis, patients the diagnosis was ‘definite neuroborreliosis’ and Missouri, United States). (S)- fingolimod phosphate all of them suffered from meningitis, which is a typical (FTY720P; B-0721) was purchased from Echelon Biosci- clinical manifestation of early LNB. The ELISA method ences Inc. (Salt Lake City, Utah, United States). Recombin- (Borrelia recombinant IgG/IgM ELISA, Vienna, Austria) and ant human plasma gelsolin (rhGSN) was obtained from immunoblotting (LINE Virotech, Rüsselsheim, Germany) Biogen-Idec Inc (Cambridge, Massachusetts, United States). were used to detect antibodies against Borrelia burgdorferi Stock solutions of S1P were prepared in 0.3 M NaOH, since in serum and CSF of LNB patients. MS patients included pH strongly affects the aggregation behavior of S1P [20]. in the study were in the process of MS diagnosis, and Various concentrations of S1P were prepared by mixing its their EDSS (Expanded Disability Status Scale) scores sonicated stock solution; 10 minutes, at room temperature were between 0.5 and 4.0 (1.7 ± 0.9). Finally, they were (RT) with buffer required for a particular experiment. diagnosed according to McDonald’s criteria
Load more

Recommended publications
  • October 2020
    PCORI Health Care Horizon Scanning System: Horizon Scanning COVID-19 Supplement Status Report Volume 1, Issue 2 Prepared for: Patient-Centered Outcomes Research Institute 1828 L St., NW, Suite 900 Washington, DC 20036 Contract No. MSA-HORIZSCAN-ECRI-ENG-2018.7.12 Prepared by: ECRI 5200 Butler Pike Plymouth Meeting, PA 19462 Investigators: Randy Hulshizer, MA, MS Marcus Lynch, PhD, MBA Jennifer De Lurio, MS Brian Wilkinson, MA Damian Carlson, MS Christian Cuevas, PhD Andrea Druga, MSPAS, PA-C Misha Mehta, MS Prital Patel, MPH Donna Beales, MLIS Eloise DeHaan, BS Eileen Erinoff, MSLIS Cassia Hulshizer, AS Madison Kimball, MS Maria Middleton, MPH Diane Robertson, BA Kelley Tipton, MPH Rosemary Walker, MLIS Karen Schoelles, MD, SM October 2020 Statement of Funding and Purpose This report incorporates data collected during implementation of the Patient-Centered Outcomes Research Institute (PCORI) Health Care Horizon Scanning System COVID-19 Supplement, operated by ECRI under contract to PCORI, Washington, DC (Contract No. MSA- HORIZSCAN-ECRI-ENG-2018.7.12). The findings and conclusions in this document are those of the authors, who are responsible for its content. No statement in this report should be construed as an official position of PCORI. An innovation that potentially meets inclusion criteria might not appear in this report simply because the horizon scanning system has not yet detected it or it does not yet meet inclusion criteria outlined in the PCORI Health Care Horizon Scanning System: Horizon Scanning Protocol and Operations Manual COVID-19 Supplement. Inclusion or absence of innovations in the horizon scanning reports will change over time as new information is collected; therefore, inclusion or absence should not be construed as either an endorsement or rejection of specific interventions.
    [Show full text]
  • Overview of Planned Or Ongoing Studies of Drugs for the Treatment of COVID-19
    Version of 16.06.2020 Overview of planned or ongoing studies of drugs for the treatment of COVID-19 Table of contents Antiviral drugs ............................................................................................................................................................. 4 Remdesivir ......................................................................................................................................................... 4 Lopinavir + Ritonavir (Kaletra) ........................................................................................................................... 7 Favipiravir (Avigan) .......................................................................................................................................... 14 Darunavir + cobicistat or ritonavir ................................................................................................................... 18 Umifenovir (Arbidol) ........................................................................................................................................ 19 Other antiviral drugs ........................................................................................................................................ 20 Antineoplastic and immunomodulating agents ....................................................................................................... 24 Convalescent Plasma ...........................................................................................................................................
    [Show full text]
  • Myosin Contraction During Apoptosis Release Damage-Associated Molecular Pattern Proteins Before Secondary Necrosis Occurs
    Cell Death and Differentiation (2013) 20, 1293–1305 OPEN & 2013 Macmillan Publishers Limited All rights reserved 1350-9047/13 www.nature.com/cdd Blebs produced by actin–myosin contraction during apoptosis release damage-associated molecular pattern proteins before secondary necrosis occurs GR Wickman1,2 3, L Julian1,2, K Mardilovich1, S Schumacher1, J Munro1, N Rath1, SAL Zander1, A Mleczak1, D Sumpton1, N Morrice1, WV Bienvenut1,4 and MF Olson*,1 Apoptosis is a fundamental homeostatic mechanism essential for the normal growth, development and maintenance of every tissue and organ. Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. We now show that during early apoptosis limited membrane permeabilization occurs in blebs and apoptotic bodies, which allows release of proteins that may affect the proximal microenvironment before the catastrophic loss of membrane integrity during secondary necrosis. Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. The most enriched proteins released were the nucleosomal histones, which have previously been identified as damage-associated molecular pattern proteins (DAMPs) that can initiate sterile inflammatory responses. These results indicate that limited membrane permeabilization occurs in blebs and apoptotic bodies before secondary necrosis, leading to acute and localized release of immunomodulatory proteins during the early phase of active apoptotic membrane blebbing.
    [Show full text]
  • Plasma Gelsolin: Indicator of Inflammation and Its Potential As a Diagnostic Tool and Therapeutic Target
    International Journal of Molecular Sciences Review Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target Ewelina Piktel 1, Ilya Levental 2, Bonita Durna´s 3, Paul A. Janmey 4 and Robert Bucki 1,* 1 Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland; [email protected] 2 McGovern Medical School, University of Texas Health Science Center, Houston MSB 4.202A, 6431 Fannin St, Houston, TX 77096, USA; [email protected] 3 Department of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Aleja IX Wieków Kielc, 25-317 Kielce, Poland; [email protected] 4 Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA; [email protected] * Correspondence: [email protected]; Tel.: +48-85-7485483 Received: 18 July 2018; Accepted: 18 August 2018; Published: 25 August 2018 Abstract: Gelsolin, an actin-depolymerizing protein expressed both in extracellular fluids and in the cytoplasm of a majority of human cells, has been recently implicated in a variety of both physiological and pathological processes. Its extracellular isoform, called plasma gelsolin (pGSN), is present in blood, cerebrospinal fluid, milk, urine, and other extracellular fluids. This isoform has been recognized as a potential biomarker of inflammatory-associated medical conditions, allowing for the prediction of illness severity, recovery, efficacy of treatment, and clinical outcome. A compelling number of animal studies also demonstrate a broad spectrum of beneficial effects mediated by gelsolin, suggesting therapeutic utility for extracellular recombinant gelsolin.
    [Show full text]
  • Therapeutic Potential of Plasma Gelsolin Administration in a Rat Model of Sepsis
    Cytokine 54 (2011) 235–238 Contents lists available at ScienceDirect Cytokine journal homepage: www.elsevier.com/locate/issn/10434666 Short Communication Therapeutic potential of plasma gelsolin administration in a rat model of sepsis Taylor S. Cohen a, Robert Bucki b, Fitzroy J. Byfield b, Nicholas J. Ciccarelli b, Brenna Rosenberg a, ⇑ Mark J. DiNubile c, Paul A. Janmey b, Susan S. Margulies a, a University of Pennsylvania, Department of Bioengineering, School of Engineering and Applied Science, 240 Skirkanich Hall, 210 S., 33rd Street, Philadelphia, PA 19104, USA b University of Pennsylvania, Institute for Medicine and Engineering, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA, 19104, USA c Department of Medical Communication, Merck Research Laboratories, West Point, PA 19486, USA article info abstract Article history: Background: Gelsolin is an actin-binding protein found in the cytoplasm and in extracellular fluids Received 15 September 2010 including blood plasma. Plasma gelsolin concentration decreases after a wide range of injuries. We Received in revised form 11 January 2011 hypothesized that the repletion of gelsolin would limit inflammation and tissue injury in a rat model Accepted 3 February 2011 of sepsis using cecal ligation and double puncture (2CLP). Available online 21 March 2011 Methods: Human plasma gelsolin (pGSN, 10 mg in 1 ml saline) was administered once immediately fol- lowing surgery, and control 2CLP (2CLP Alb) and sham animals were injected with 1 ml saline containing Keywords: equimolar albumin. Treatments were administered intraperitoneally (IP), intravenously (IV), or subcuta- Gelsolin neously (SC). Sepsis Tissue injury Results: Gelsolin levels in the 2CLP Alb group were lower than in sham animals.
    [Show full text]
  • Bioaegis Therapeutics ID Week Poster
    BioAegis Therapeutics and the T.H. Chan-Harvard School of Public Health Demonstrate “Delayed Therapy with Plasma Gelsolin Improves Survival in Murine Pneumococcal Pneumonia” at ID Week MORRISTOWN, NJ and BOSTON, MA, (BIOAEGIS THERAPEUTICS) October 18, 2017 BioAegis Therapeutics Inc., a privately held biotechnology company exploiting the role of plasma gelsolin (pGSN) in augmenting innate immunity, announced that data demonstrating that “Delayed Therapy with Plasma Gelsolin Improves Survival in Murine Pneumococcal Pneumonia” was presented during ID Week, on October 4-8, 2017 in San Diego, California. ID Week is the annual meeting sponsored by the IDSA, PIDSA, HIVMA & SHEA, organizations which represent infectious disease physicians and health care professionals. Studies funded by a $2.8 MM NIH partnership grant with the T.H. Chan Harvard School of Public Health demonstrated that recombinant human plasma gelsolin (rhu-pGSN) therapy can substantially improve survival in a highly lethal murine model of pneumococcal pneumonia, even when initiated 2 and 3 days after infectious challenge and in the absence of antibiotics. The paradigm differs from most animal models of infectious disease where therapies are administered before or shortly after the infectious challenge. The specific design in these experiments more closely reflects the clinical reality of real-world patients who do not present for treatment until symptoms develop. In the experiments presented at ID Week, the mice exhibited signs of illness prior to therapy, and typically died
    [Show full text]
  • Secretion of Amyloidogenic Gelsolin Progressively Compromises Protein Homeostasis Leading to the Intracellular Aggregation of Proteins
    Secretion of amyloidogenic gelsolin progressively compromises protein homeostasis leading to the intracellular aggregation of proteins Lesley J. Pagea, Ji Young Sukb,1, Lyudmila Bazhenovab,1, Sheila M. Flemingc, Malcolm Wooda, Yun Jiangd, Ling T. Guod, Andrew P. Mizisind, Robert Kisilevskye, G. Diane Sheltond, William E. Balcha,f,g,h,2, and Jeffery W. Kellyb,h,2 Department of aCell Biology, bDepartments of Chemistry and Molecular and Experimental Medicine, fDepartment of Chemical Physiology, and gInstitute for Childhood and Neglected Diseases, and hThe Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037; cDepartment of Neurobiology, The David Geffen School of Medicine, University of California, Los Angeles, CA 90095; dDepartment of Pathology, University of California at San Diego, La Jolla, CA 92093; and eDepartments of Pathology and Molecular Medicine, and Biochemistry, Queen’s University, Kingston, ON, Canada K7L 3N6 Edited by Charles Weissmann, Scripps Florida, Jupiter, FL, and approved May 1, 2009 (received for review November 20, 2008) Familial amyloidosis of Finnish type (FAF) is a systemic amyloid 18). Given that human D187N/Y plasma gelsolin is expressed in disease associated with the deposition of proteolytic fragments of most tissues and that the C68 fragment is in the blood, the origin mutant (D187N/Y) plasma gelsolin. We report a mouse model of of the amyloidogenic fragments (local production versus serum- FAF featuring a muscle-specific promoter to drive D187N gelsolin derived) has been unclear, as are the events contributing to synthesis. This model recapitulates the aberrant endoproteolytic age-onset proteotoxicity. cascade and the aging-associated extracellular amyloid deposition Herein, we report transgenic human D187N gelsolin amyloid- of FAF.
    [Show full text]
  • Ceragenins As Mimics of Endogenous Antimicrobial Peptides
    ntimicrob A ia f l o A l g a e n n r Journal of t u s o J Hashemi et al., J Antimicrob Agents 2017, 3:2 DOI: 10.4172/2472-1212.1000141 ISSN: 2472-1212 Antimicrobial Agents Review Article Open Access Ceragenins as Mimics of Endogenous Antimicrobial Peptides Marjan M Hashemi1, Brett S Holden1, Bonita Durnaś2, Robert Bucki3 and Paul B Savage1* 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, USA 2Department of Microbiology and Immunology, The Faculty of Health Sciences, Jan Kochanowski University, Kielce, Poland 3Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Poland *Corresponding author: Paul B Savage, Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, USA, Tel: 1 801 422 4020; Fax: 1 801 422 0153; E-mail: [email protected] Received date: April 18, 2017; Accepted date: May 10, 2017; Published date: May 17, 2017 Copyright: © 2017 Hashemi MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Ceragenins are small molecule mimics of endogenous antimicrobial peptides (AMPs), and as such display broad- spectrum antimicrobial activity. These molecules are derived from a common bile acid and can be prepared at a large scale. Because ceragenins are not peptide based, they are not substrates for proteases. Gram-negative and positive bacteria are susceptible to ceragenins, including drug resistant organisms. Although ceragenins and colistin have common features, ceragenins retain full antibacterial activity against colistin-resistant Gram-negative bacteria.
    [Show full text]
  • Role of Plasma Gelsolin Protein in the Final Stage of Erythropoiesis and in Correction of Erythroid Dysplasia in Vitro
    International Journal of Molecular Sciences Article Role of Plasma Gelsolin Protein in the Final Stage of Erythropoiesis and in Correction of Erythroid Dysplasia In Vitro So Yeon Han 1,2, Eun Mi Lee 2, Suyeon Kim 1 , Amy M. Kwon 3 and Eun Jung Baek 1,2,* 1 Department of Laboratory Medicine, College of Medicine, Hanyang University, Seoul 04763, Korea; [email protected] (S.Y.H.); [email protected] (S.K.) 2 Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea; ghlfl[email protected] 3 Biostatistical Consulting and Research Laboratory, Medical Research Collaborating Center, Industry-University Cooperation Foundation, Hanyang University, Seoul 04763, Korea; [email protected] * Correspondence: [email protected]; Tel.: +82-31-560-2485; Fax: +82-31-560-2489 Received: 10 September 2020; Accepted: 25 September 2020; Published: 27 September 2020 Abstract: Gelsolin, an actin-remodeling protein, is involved in cell motility, cytoskeletal remodeling, and cytokinesis and is abnormally expressed in many cancers. Recently, human recombinant plasma gelsolin protein (pGSN) was reported to have important roles in cell cycle and maturation of primary erythroblasts. However, the role of human plasma gelsolin in late stage erythroblasts prior to enucleation and putative clinical relevance in patients with myelodysplastic syndrome (MDS) and hemato-oncologic diseases have not been reported. Polychromatic and orthochromatic erythroblasts differentiated from human cord blood CD34+ cells, and human bone marrow (BM) cells derived from patients with MDS, were cultured in serum-free medium containing pGSN. Effects of pGSN on mitochondria, erythroid dysplasia, and enucleation were assessed in cellular and transcriptional levels.
    [Show full text]
  • Plasma Gelsolin and Matrix Metalloproteinase 3 As Potential Biomarkers for Alzheimer Disease
    Neuroscience Letters 595 (2015) 116–121 Contents lists available at ScienceDirect Neuroscience Letters journal homepage: www.elsevier.com/locate/neulet Research article Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease Mao Peng a,b,c,d, Jianping Jia a,b,c,d,∗, Wei Qin b,c,d a Department of Neurology, Xuan Wu Hospital of the Capital Medical University, Beijing, China b Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing, China c Beijing Key Laboratory of Geriatric Cognitive Disorders, China d Key Neurodegenerative Laboratory of Ministry of Education of the People’s Republic of China, Beijing, China highlights • GSN levels were decreased in plasma of AD. • MMP3 activity were increased in plasma of AD. • Both the GSN level and MMP3 activity correlated with the MMSE scores. • Combination of GSN level, MMP3 activity and clinical data may help in screening AD. article info abstract Article history: Gelsolin (GSN) levels and matrix metalloproteinase 3 (MMP3) activity have been found to be altered in Received 5 December 2014 the plasma in patients with Alzheimer disease (AD). The aim of this study was to determine whether Received in revised form 20 February 2015 a combination of these proteins with clinical data is specific and sensitive enough for AD diagnosis. In Accepted 7 April 2015 113 non-demented controls and 113 patients with probable AD, the plasma GSN levels were determined Available online 9 April 2015 using the enzyme-linked immunosorbent assay (ELISA), and the plasma MMP3 activity was determined using casein zymography. Logistic regression and receiver operating characteristic (ROC) curve analysis Keywords: were used to determine the diagnostic accuracy of these proteins combined with clinical data.
    [Show full text]
  • Interaction of the Gelsolin-Derived Antibacterial PBP 10 Peptide with Lipid Bilayers and Cell Membranes
    University of Pennsylvania ScholarlyCommons Institute for Medicine and Engineering Papers Institute for Medicine and Engineering September 2006 Interaction of the Gelsolin-Derived Antibacterial PBP 10 Peptide with Lipid Bilayers and Cell Membranes Robert Bucki University of Pennsylvania, [email protected] Paul Janmey University of Pennsylvania, [email protected] Follow this and additional works at: https://repository.upenn.edu/ime_papers Bucki, Robert and Janmey, Paul, "Interaction of the Gelsolin-Derived Antibacterial PBP 10 Peptide with Lipid Bilayers and Cell Membranes" (2006). Institute for Medicine and Engineering Papers. 25. https://repository.upenn.edu/ime_papers/25 Reprinted from Antimicrobial Agents & Chemotherapy, Volume 50, Number 9, September 2006, pages 2932-40. Publisher URL: http://aac.asm.org/cgi/reprint/50/9/2932.pdf This paper is posted at ScholarlyCommons. https://repository.upenn.edu/ime_papers/25 For more information, please contact [email protected]. Interaction of the Gelsolin-Derived Antibacterial PBP 10 Peptide with Lipid Bilayers and Cell Membranes Abstract PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. Isolated LPS and LTA decrease the antimicrobial activities of PBP 10, as well as other antimicrobial peptides, such as cathelicidin-LL37 (LL37) and mellitin. In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides.
    [Show full text]
  • COVID-19 Update: the Race to Therapeutic Development
    Journal Pre-proof COVID-19 update: The race to therapeutic development Julianne D. Twomey, Shen Luo, Alexis Q. Dean, William P. Bozza, Ancy Nalli, Baolin Zhang PII: S1368-7646(20)30063-7 DOI: https://doi.org/10.1016/j.drup.2020.100733 Reference: YDRUP 100733 To appear in: Drug Resistance Updates Received Date: 15 September 2020 Revised Date: 19 October 2020 Accepted Date: 21 October 2020 Please cite this article as: { doi: https://doi.org/ This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2020 Published by Elsevier. COVID-19 update: The race to therapeutic development Julianne D. Twomey, Shen Luo, Alexis Q. Dean, William P. Bozza, Ancy Nalli, and Baolin Zhang* Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, United States All authors contributed equally and agreed to the content of the manuscript. *Correspondence: Baolin Zhang, Ph.D., 240-402-6740, E-mail: [email protected] Abstract The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2), represents an unprecedented challenge to global public health.
    [Show full text]