Protein Expression in Mammalian Cell Lines After Lowlevel Metal Exposure
Montana Tech Library Digital Commons @ Montana Tech Graduate Theses & Non-Theses Student Scholarship Spring 2021 PROTEIN EXPRESSION IN MAMMALIAN CELL LINES AFTER LOWLEVEL METAL EXPOSURE Sydney Jennings Follow this and additional works at: https://digitalcommons.mtech.edu/grad_rsch PROTEIN EXPRESSION IN MAMMALIAN CELL LINES AFTER LOW- LEVEL METAL EXPOSURE by Sydney Jennings A thesis submitted in partial fulfillment of the requirements for the degree of Interdisciplinary Masters of Science Montana Tech 2021 ii Abstract Mining in Butte, Montana has been ongoing since the mid-19th century. The US Environmental Protection Agency (EPA) added the Butte Area to the National Priority List in 1983, designating it a Superfund site. Butte is currently part of the largest EPA Superfund site in the United States. The EPA lists arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg) as metal contaminants of concern for residents living in proximity to the Butte Area Superfund site. However, very limited human biomonitoring has been conducted in Butte and studies that have been published focus on Pb and, to a lesser extent, As. No synergistic, antagonistic, or additive studies have been conducted, even though it is widely accepted that the exposure in Butte is a metal mixture scenario, rather than single element exposure. Metals that are trace micronutrients, such as copper (Cu), manganese (Mn), and zinc (Zn) have been largely unrecognized as possibly having negative health effects on residents of Butte, despite the fact the metals have been historically released into the soil, water, and air through active blasting and crushing of ore and are known to be potential neurotoxins.
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