Product datasheet Anti-MCM7 Antibody Catalog Number: PB9261
BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China
Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]
Basic Information Product Name Anti-MCM7 Antibody Gene Name MCM7 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,IHC-F,ICC/IF,FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7. Purification Immunogen affinity purified. Observed MW 81KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 Immunohistochemistry(Frozen Section): 1:50-400 Immunocytochemistry/Immunofluorescence: 1:50-400 Flow Cytometry: 1-3μg/1x106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing
Background Information MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini- chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MCM7 Antibody Catalog Number: PB9261
BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China
Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]
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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-MCM7 Antibody Catalog Number: PB9261
BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China
Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]
Selected Validation Data
Figure 1. Western blot Figure 2. Western blot analysis of MCM7 using analysis of MCM7 using anti-MCM7 antibody anti-MCM7 antibody (PB9261). Electrophoresis (PB9261). Electrophoresis was performed on a was performed on a 5-20% SDS-PAGE gel at 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V 70V (Stacking gel) / 90V (Resolving gel) for 2-3 (Resolving gel) for 2-3 hours.Lane 1: hours. The sample well of Recombinant Human each lane was loaded MCM7 Protein 0.5ng with 50ug of sample After Electrophoresis, under reducing proteins were transferred conditions. Lane 1: Rat to a Nitrocellulose Brain Tissue LysateLane 2: membrane at 150mA for Human Placenta Tissue 50-90 minutes. Blocked LysateLane 3: NIH3T3 the membrane with 5% Whole Cell LysateLane 4: Non-fat Milk/ TBS for 1.5 HELA Whole Cell hour at RT. The LysateLane 5: JURKAT membrane was incubated Whole Cell LysateLane 6: with rabbit anti-MCM7 22RV1 Whole Cell antigen affinity purified LysateLane 7: COLO320 polyclonal antibody Whole Cell LysateLane 8: (Catalog # PB9261) at 0.5 PC-12 Whole Cell Lysate μg/mL overnight at 4°C, After Electrophoresis, then washed with proteins were transferred TBS-0.1%Tween 3 times to a Nitrocellulose with 5 minutes each and membrane at 150mA for probed with a goat anti- 50-90 minutes. Blocked rabbit IgG-HRP the membrane with 5% secondary antibody at a Non-fat Milk/ TBS for 1.5 dilution of 1:10000 for 1.5 hour at RT. The hour at RT. The signal is membrane was incubated developed using an with rabbit anti-MCM7 Enhanced antigen affinity purified Chemiluminescent polyclonal antibody detection (ECL) kit (Catalog # PB9261) at 0.5 (Catalog # EK1002) with μg/mL overnight at 4°C, Tanon 5200 system. A then washed with specific band was TBS-0.1%Tween 3 times detected for MCM7 at with 5 minutes each and approximately 39KD. The probed with a goat anti- expected band size for rabbit IgG-HRP MCM7 is at 39KD. secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3 Product datasheet Anti-MCM7 Antibody Catalog Number: PB9261
BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China
Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]
(Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81KD. The expected band size for MCM7 is at 81KD.
Figure 3. IHC analysis of Figure 4. IHC analysis of MCM7 using anti-MCM7 MCM7 using anti-MCM7 antibody (PB9261).MCM7 antibody (PB9261).MCM7 was detected in paraffin- was detected in paraffin- embedded section of embedded section of rat mouse testis tissue. Heat intestine tissue. Heat mediated antigen mediated antigen retrieval was performed retrieval was performed in citrate buffer (pH6, in citrate buffer (pH6, epitope retrieval solution) epitope retrieval solution) for 20 mins. The tissue for 20 mins. The tissue section was blocked with section was blocked with 10% goat serum. The 10% goat serum. The tissue section was then tissue section was then incubated with 1μg/ml incubated with 1μg/ml rabbit anti-MCM7 rabbit anti-MCM7 Antibody (PB9261) Antibody (PB9261) overnight at 4°C. overnight at 4°C. Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody and secondary antibody and incubated for 30 minutes incubated for 30 minutes at 37°C. The tissue at 37°C. The tissue section was developed section was developed using Strepavidin-Biotin- using Strepavidin-Biotin- Complex (SABC)(Catalog Complex (SABC)(Catalog # SA1022) with DAB as # SA1022) with DAB as the chromogen. the chromogen.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 4
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