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Blood Collection
Blood Collection (Note: Navigation around this large pdf document is best accomplished using the bookmarks function.) 355.1 Preface Blood collection (venipuncture, phlebotomy) is a common and important specimen collection procedure in the conduct of research. In many protocols, multiple blood draws are an important part of collecting and analyzing data. The Emory University Institutional Animal Care and Use Committee (IACUC) developed a policy to best enable blood collection while minimizing the potential for pain, unnecessary stress, distress or untoward effect in research animals. These are articulated by way of this general overview supplemented by companion documents appropriate to certain species. The species-specific sections differentiate from the general standards in being more precise, and sometimes more adaptable, in considering the frequency and total number of blood collection events; maximum collectable volumes allowed based upon specific physiology; detailing allowable routes particular to each species; differentiating between terminal and survival circumstances; disclosing requirements for anesthesia or restraint; scientific qualifiers and addressing conditionally permissible methods or settings germane to a species. This list is not exhaustive and persons requiring information regarding the supplies and equipment needed, specifics of restraint or anesthesia, requirements for ancillary care, habituation requirements, application to study in the field and other information are encouraged to contact the Training Coordinators for their specific site. o DAR Training Request: http://www.dar.emory.edu/forms/training_wrkshp.php o Yerkes National Primate Research Center Training: Jennifer McMillan, [email protected], 404-712-9217 While it only takes about 24 hours for the lost fluid volume of blood to be restored, it takes longer to regeneratively replenish erythrocytes, platelets and other circulating factors. -
Evidence-Based Treatment Planning for the Restoration of Endodontically
RESTORATIVE DENTISTRY Evidence-based treatment planning for the restoration of endodontically treated single teeth: importance of coronal seal, post vs no post, and indirect vs direct restoration Alan Atlas, DMD/Simone Grandini, DDS, MSc, PhD/Marco Martignoni, DMD Every orthograde endodontic procedure requires restoration endodontically treated teeth or not are inconclusive. For dental of the coronal (access) cavity. The specific type of treatment practitioners, this is not a satisfactory result. This appraisal eval- used in individual cases greatly depends on the amount and uates available evidence and trends for coronal restoration of configuration of the residual coronal tooth structure. In prac- single endodontically treated teeth with a focus on clinical in- tice there are Class I access cavities as well as coronally severely vestigations, where available. It provides specific recommenda- damaged, even decapitated, teeth and all conceivable manifes- tions for their coronal restoration to assist clinicians in their tations in between. The latest attempts to review results from decision making and treatment planning. (Quintessence Int clinical trials to answer the question of whether post place- 2019;50: 772–781; doi: 10.3290/j.qi.a43235) ment or crowning can be recommended for the restoration of Key words: coronal restoration, direct restoration, endodontically treated teeth (ETT), endodontics, fiber post, indirect restoration, seal Every orthograde endodontic procedure requires restoration of The importance of coronal restoration for the coronal (access) cavity. The specific type of treatment used endodontic treatment outcome in individual cases greatly depends on the amount and config- uration of the residual coronal tooth structure. In practice there Leaking coronal restorations dramatically reduce the chance of are Class I access cavities as well as coronally severely damaged, endodontic treatment success. -
Incidence of Post Cross Clamp Ventricular Fibrillation in Isolated Coronary Artery Bypass Surgery Using Del Nido Cardioplegia and Conventional Blood Cardioplegia
Jemds.com Original Research Article Incidence of Post Cross Clamp Ventricular Fibrillation in Isolated Coronary Artery Bypass Surgery Using del Nido Cardioplegia and Conventional Blood Cardioplegia Biju Kambil Thyagarajan1, Anandakuttan Sreenivasan2, Ravikrishnan Jayakumar3, Ratish Radhakrishnan4 1, 2, 3, 4 Department of Cardiovascular and Thoracic Surgery, Government T D Medical College, Alappuzha, Kerala, India. ABSTRACT BACKGROUND The cardiac surgical procedures and surgical outcomes witnessed a dramatic Corresponding Author: improvement with the introduction of cardiopulmonary bypass and cardioplegia Dr. Anandakuttan Sreenivasan, techniques. Ventricular fibrillation immediately after removal of aortic cross clamp is Department of Cardiovascular and an energy consuming process leading to myocardial injury in an already energy Thoracic Surgery, Government T D Medical College, Alappuzha, Kerala, India, depleted heart. Electrical cardioversion, which itself causes myocardial injury, is E-mail: [email protected] required to regain normal rhythm. Prevention of ventricular fibrillation is important in preventing myocardial injury. We retrospectively analysed the incidence of post DOI: 10.14260/jemds/2020/797 cross clamp ventricular fibrillation requiring electrical defibrillation in isolated coronary artery bypass surgery using del Nido cardioplegia and conventional blood How to Cite This Article: cardioplegia. Thyagarajan BK, Sreenivasan A, Jayakumar R, et al. Incidence of post cross clamp METHODS ventricular fibrillation in isolated coronary -
A Clever Technique for Placement of a Urinary Catheter Over a Wire
[Downloaded free from http://www.urologyannals.com on Monday, August 24, 2015, IP: 107.133.192.199] Original Article A clever technique for placement of a urinary catheter over a wire Joel E. Abbott, Adam Heinemann, Robert Badalament, Julio G. Davalos1 Department of Urology, St. John Providence, Michigan State University, Detroit, MI 48071, 1Chesapeake Urology Associates, University of Maryland, Baltimore, MD 21061, USA Abstract Objective: The objective was to present a straightforward, step-by-step reproducible technique for placement of a guide-wire into any type of urethral catheter, thereby offering a means of access similar to that of a council-tip in a situation that may require a different type of catheter guided over a wire. Materials and Methods: Using a shielded intravenous catheter inserted into the eyelet of a urinary catheter and through the distal tip, a “counsel-tip” can be created in any size or type of catheter. Once transurethral bladder access has been achieved with a hydrophilic guide-wire, this technique will allow unrestricted use of catheters placed over a wire facilitating guided catheterization. Results: Urethral catheters of different types and sizes are easily advanced into the bladder with wire-guidance; catheterization is improved in the setting of difficult urethral catheterization (DUC). Cost analysis demonstrates benefit overuse of traditional council-tip catheter. Conclusion: Placing urinary catheters over a wire is standard practice for urologists, however, use of this technique gives the freedom of performing wire-guided catheterization in more situations than a council-tip allows. This technique facilitates successful transurethral catheterization over wire in the setting of DUC for all catheter types and styles aiding in urologic management of patients at a cost benefit to the health care system. -
CMS Manual System Human Services (DHHS) Pub
Department of Health & CMS Manual System Human Services (DHHS) Pub. 100-07 State Operations Centers for Medicare & Provider Certification Medicaid Services (CMS) Transmittal 8 Date: JUNE 28, 2005 NOTE: Transmittal 7, of the State Operations Manual, Pub. 100-07 dated June 27, 2005, has been rescinded and replaced with Transmittal 8, dated June 28, 2005. The word “wound” was misspelled in the Interpretive Guidance section. All other material in this instruction remains the same. SUBJECT: Revision of Appendix PP – Section 483.25(d) – Urinary Incontinence, Tags F315 and F316 I. SUMMARY OF CHANGES: Current Guidance to Surveyors is entirely replaced by the attached revision. The two tags are being combined as one, which will become F315. Tag F316 will be deleted. The regulatory text for both tags will be combined, followed by this revised guidance. NEW/REVISED MATERIAL - EFFECTIVE DATE*: June 28, 2005 IMPLEMENTATION DATE: June 28, 2005 Disclaimer for manual changes only: The revision date and transmittal number apply to the red italicized material only. Any other material was previously published and remains unchanged. However, if this revision contains a table of contents, you will receive the new/revised information only, and not the entire table of contents. II. CHANGES IN MANUAL INSTRUCTIONS: (N/A if manual not updated.) (R = REVISED, N = NEW, D = DELETED) – (Only One Per Row.) R/N/D CHAPTER/SECTION/SUBSECTION/TITLE R Appendix PP/Tag F315/Guidance to Surveyors – Urinary Incontinence D Appendix PP/Tag F316/Urinary Incontinence III. FUNDING: Medicare contractors shall implement these instructions within their current operating budgets. IV. ATTACHMENTS: Business Requirements x Manual Instruction Confidential Requirements One-Time Notification Recurring Update Notification *Unless otherwise specified, the effective date is the date of service. -
Introduction to Neuroimaging
Introduction to Neuroimaging Aaron S. Field, MD, PhD Assistant Professor of Radiology Neuroradiology Section University of Wisconsin–Madison Updated 7/17/07 Neuroimaging Modalities Radiography (X-Ray) Magnetic Resonance (MR) Fluoroscopy (guided procedures) • MR Angiography/Venography (MRA/MRV) • Angiography • Diffusion and Diffusion Tensor • Diagnostic MR • Interventional • Perfusion MR • Myelography • MR Spectroscopy (MRS) Ultrasound (US) • Functional MR (fMRI) • Gray-Scale Nuclear Medicine ―Duplex‖ • Color Doppler • Single Photon Emission Computed Tomography (SPECT) Computed Tomography (CT) • Positron Emission Tomography • CT Angiography (CTA) (PET) • Perfusion CT • CT Myelography Radiography (X-Ray) Radiography (X-Ray) Primarily used for spine: • Trauma • Degenerative Dz • Post-op Fluoroscopy (Real-Time X-Ray) Fluoro-guided procedures: • Angiography • Myelography Fluoroscopy (Real-Time X-Ray) Fluoroscopy (Real-Time X-Ray) Digital Subtraction Angiography Fluoroscopy (Real-Time X-Ray) Digital Subtraction Angiography Digital Subtraction Angiography Indications: • Aneurysms, vascular malformations and fistulae • Vessel stenosis, thrombosis, dissection, pseudoaneurysm • Stenting, embolization, thrombolysis (mechanical and pharmacologic) Advantages: • Ability to intervene • Time-resolved blood flow dynamics (arterial, capillary, venous phases) • High spatial and temporal resolution Disadvantages: • Invasive, risk of vascular injury and stroke • Iodinated contrast and ionizing radiation Fluoroscopy (Real-Time X-Ray) Myelography Lumbar or -
Deep Venous Thrombosis with Suspected Pulmonary Embolism
Thoracic Imaging Peter A. Loud, MD Deep Venous Thrombosis Douglas S. Katz, MD Dennis A. Bruce, MD with Suspected Pulmonary Donald L. Klippenstein, MD Zachary D. Grossman, MD Embolism: Detection with Combined CT Venography Index terms: Computed tomography (CT), 1 angiography, 9*.129142, and Pulmonary Angiography 9*.12915, 9*.12916 Embolism, pulmonary, 60.72 Pulmonary angiography, 944.12914, 944.12915, 944.12916 PURPOSE: To determine the frequency and location of deep venous thrombosis at Veins, thrombosis, 9*.751, 9*.12914 computed tomographic (CT) venography after CT pulmonary angiography in a large series of patients clinically suspected of having pulmonary embolism and to Radiology 2001; 219:498–502 compare the accuracy of CT venography with lower-extremity venous sonography. Abbreviation: MATERIALS AND METHODS: Venous phase images were acquired from the DVT ϭ deep venous thrombosis diaphragm to the upper calves after completion of CT pulmonary angiography in 650 patients (373 women, 277 men; age range, 18–99 years; mean age, 63 years) 1 From the Department of Radiology, to determine the presence and location of deep venous thrombosis. Results of CT Roswell Park Cancer Institute, Elm and Carlton Sts, Buffalo, NY 14263 (P.A.L., venography were compared with those of bilateral lower-extremity venous sonog- D.L.K., Z.D.G.), and the Department raphy in 308 patients. of Radiology, Winthrop University Hospital, Mineola, NY (D.S.K., D.A.B.). RESULTS: A total of 116 patients had pulmonary embolism and/or deep venous From the 1999 RSNA scientific assem- thrombosis, including 27 patients with pulmonary embolism alone, 31 patients with bly. -
Myelography in the Assessment of Degenerative Lumbar Scoliosis And
https://doi.org/10.14245/kjs.2017.14.4.133 KJS Print ISSN 1738-2262 On-line ISSN 2093-6729 CLINICAL ARTICLE Korean J Spine 14(4):133-138, 2017 www.e-kjs.org Myelography in the Assessment of Degenerative Lumbar Scoliosis and Its Influence on Surgical Management George McKay, Objective: Myelography has been shown to highlight foraminal and lateral recess stenosis more Peter Alexander Torrie, readily than computed tomography (CT) or magnetic resonance imaging (MRI). It also has the Wendy Bertram, advantage of providing dynamic assessment of stenosis in the loaded spine. The advent of weight-bearing MRI may go some way towards improving assessment of the loaded spine Priyan Landham, and is less invasive, however availability remains limited. This study evaluates the potential Stephen Morris, role of myelography and its impact upon surgical decision making. John Hutchinson, Methods: Of 270 patients undergoing myelography during 2006-2009, a period representing Roland Watura, peak utilisation of this imaging modality in our unit, we identified 21 patients with degenerative Ian Harding scoliosis who fulfilled our inclusion criteria. An operative plan was formulated by our senior author based initially on interpretation of an MRI scan. Subsequent myelogram and CT myelogram Department of Spinal Surgery, investigations were scrutinised, with any additional abnormalities noted and whether these im- Southmead Hospital, Bristol, United pacted upon the operative plan. Kingdom Results: From our 21 patients, 18 (85.7%) had myelographic findings not identified on MRI. Of Corresponding Author: note, in 4 patients, supine CT myelography yielded additional information when compared to George McKay supine MRI in the same patients. -
Distinguished Lecture
Past Present and Future of Joint Manipulation Stanley V. Paris PT., PhD, FAPTA N.Z.S.P., F.N.Z.S.P.(Hon Fellow & Life).,, N.Z.M.T.A.,(Hon Life)., I.F.O.M.P.T.,(Hon Life)., F.A.A.O.M.P.T., M.C.S.P., B.I.M. Abstract: Presented as the first Distinguished Lecturers Award, of the American Academy of Orthopaedic Manipulative Physical Therapists October 2011, the paper begins by addressing the richness of manipulative experience that caused the Founding Fellows to create the Academy. Speaking to his concerns that this richness seems to be forgotten by many practitioners he reviewed also the known effects of manipulation before then evaluating the evidence based literature criticizing much of it for being too basic and taking the profession back to where we were some fifty years ago before specific manipulative techniques were in vogue. Thus the current research is largely on non- specific regional techniques done for effect rather than for pathoanatomical and mechanical consideration. Many of the techniques being studied and promoted as manipulations ion the current literature do not justify to be called “manipulations” lacking as they do “skilled passive movements to a joint.” The paper argues for remembering that published literature is only one leg of the three legged stool of evidenced based practice, the other legs being patients wishes and culture, and the third being individual therapists expertise. Given the quality of much current physical therapy evidenced based literature Dr. Paris did not think that it was of sufficient scope and quality on which to base our practice. -
Digital Vein Mapping Guiding Laser and Injection Sclerotherapy to Treat Telangiectasias and Feeder Veins: Report of 140 Cases
ARTÍCULO ORIGINAL Digital Vein Mapping Guiding Laser and Injection sclerotherapy to treat telangiectasias and Feeder Veins: Report of 140 Cases. Authors: Roberto Kasuo Miyake, MD, PhD / Rodrigo Kikuchi, MD / Flavio Henrique Duarte, MD / John Davidson, MD / Hiroshi Miyake, MD, PhD Institution: Clinica Miyake, Sao Paulo, Brazil E-Mail autor: [email protected] Fecha recepción artículo: 23/11/2008 - Fecha aceptación artículo: Marzo 2009 Abstract Resumen Background and Objective: Telangiectasias Antecedentes y Objetivo: la ineficiencia en el treatment inefficiency may occur due to tratamiento de las telangiectasias puede deber- invisible feeder veins. These veins can be made se a las venas nutricias no visibles. Estas venas discernible by use of a digital vein detection pueden hacerse perceptibles mediante el uso de device (V-V). This study evaluates a method un dispositivo digital de detección (VV). Este to treat vascular lesions by combining 1064nm estudio evalúa un método para el tratamiento laser, injection sclerotherapy, a skin cooling de lesiones vasculares mediante la combinación device (CLaCS) and the V-V. de láser de 1064nm, la escleroterapia por inyec- Materials and Methods: Patients were treated ción y un dispositivo de enfriamiento de la piel with laser and sclerotherapy, both applied (CLACS, Cryo-Laser y Cryo-Sclerotherapy)) y under cooling. V-V was used to visualize hidden el VV (The VeinViewer™). feeder veins. Material y Métodos: Los pacientes fueron trata- Results: A total of 140 patients underwent dos con láser y la escleroterapia, ambos aplicados CLaCS. In 121 patients (86%) satisfactory bajo enfriamiento con (CLaCS). El dispositivo cosmetic results were obtained avoiding digital V-V se utiliza para visualizar las venas phlebectomy. -
High Resolution Anoscopy Overview
High Resolution Anoscopy Overview Naomi Jay, RN, NP, PhD University of California San Francisco Email: [email protected] Disclosures No Disclosures Definition of HRA Examination of the anus, anal canal and perianus using a colposcope with 5% acetic acid and Lugol’s solution. Basic Principles • Office-based procedure • Adapted from gynecologic colposcopy. • Validated for anal canal. • Similar terminology and descriptors. may be unfamiliar to non-gyn providers. • Comparable to vaginal and vulvar colposcopy. • Clinicians familiar with cervical colposcopy may be surprised by the difficult transition. Anal SCJ & AnTZ • Original vs. current SCJ less relevant. • TZ features less common, therefore more difficult to appreciate. • SCJ more subtle, difficult to see in entirety requires more manipulation & acetic acid. • Larger area of metaplastic changes overlying columnar epithelium compared to endocervix. • Most lesions found in the AnTZ. Atypical Metaplasia • Atypical metaplasia may indicate the presence of HSIL. • Radiate over distal rectum from SCJ. • Thin, may wipe off. • Features to look for indicating potential lesions: • Atypical clustered glands (ACG) • Lacy metaplastic borders (LM) • Epithelial Honeycombing (EH) Lugol’s. Staining • More utility in anus compared to cervix. • Adjunctive to help define borders, distinguish between possible LSIL/HSIL. • Most HSIL will be Lugol’s negative • LSIL may be Lugol’s partial or negative • Applied focally with small cotton swabs to better define an acetowhite lesion. •NOT a short cut to determine presence or absence of lesions, acetic acid is used first and is applied frequently. Anal vs. Cervical Characteristics • Punctation & Mosaic rarely “fine” mostly “coarse”. • Mosaic pattern mostly associated with HSIL. • Atypical vessels may be HSIL or cancer • Epithelial honeycombing & lacy metaplasia unique anal descriptors. -
Concept of Occlusion for Dental Restoration and Occlusal Rehabilitation - an Overview
Overview Overview Concept of occlusion for dental restoration and occlusal rehabilitation - an overview Dr. Yuh-Yuan Shiau Abstract Professor Emeritus, School of Dentistry, National Taiwan Restoring defects on teeth is a daily practice of a dental University practitioner. However, the proper restoration of the destructed National Taiwan University Hospital occlusal surfaces should not jeopardize the occlusal scheme that Department of Dentistry, #1, Chang-Teh Street, Taipei, Taiwan, 100 a patient already has. Therefore, the restored occlusal surfaces should be able to maintain the occlusal scheme that exsisted Corresponding author: before the treatment. However, if the overall dentition is to be Yuh-Yuan Shiau, DDS, MS, MFICD reconstructed due to loss of too many teeth, severe attrition or Professor emeritus, School of Dentistry, an improper jaw position, or the occlusal form of majority teeth National Taiwan University, Taiwan of one jaw or both jaws needing to be changed, then an ideal Department of Dentistry, National Taiwan University Hospital, occlusal form including point centric occlusion, canine guidance, No.1, Chang-Teh Street, Taipei, Taiwan, 100 posterior eccentric disclusion, etc. should be provided according E-mail: [email protected] to the demands of the patient and esthetic and functional expectations of the dentist. Computer-aided techniques for the DOI: 10.6926/JPI.201907_8(3).0001 construction of occlusal surfaces may enhance the production of said occlusal forms, yet properly applying the concepts for either dental restoration or occlusal rehabilitation remain the key to success. Key words: dental restoration, occlusal rehabilitation, ideal occlusal form, computer-aided techniques Introduction The restoration of destructed teeth caused by dental caries or fractures of parts of the coronal dental structures is a common daily work of a dentist.