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Pan-HDAC Inhibitors Restore PRDM1 Response to IL21 in CREBBP

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Pan-HDAC Inhibitors Restore PRDM1 Response to IL21 in CREBBP Published OnlineFirst October 22, 2018; DOI: 10.1158/1078-0432.CCR-18-1153 Cancer Therapy: Preclinical Clinical Cancer Research Pan-HDAC Inhibitors Restore PRDM1 Response to IL21 in CREBBP-Mutated Follicular Lymphoma Fabienne Desmots1,2, Mikael€ Roussel1,2,Celine Pangault1,2, Francisco Llamas-Gutierrez3,Cedric Pastoret1,2, Eric Guiheneuf2,Jer ome^ Le Priol2, Valerie Camara-Clayette4, Gersende Caron1,2, Catherine Henry5, Marc-Antoine Belaud-Rotureau5, Pascal Godmer6, Thierry Lamy1,7, Fabrice Jardin8, Karin Tarte1,9, Vincent Ribrag4, and Thierry Fest1,2 Abstract Purpose: Follicular lymphoma arises from a germinal cen- is enriched in the potent inducer of PRDM1 and IL21, highly ter B-cell proliferation supported by a bidirectional crosstalk produced by Tfhs. In follicular lymphoma carrying CREBBP with tumor microenvironment, in particular with follicular loss-of-function mutations, we found a lack of IL21-mediated helper T cells (Tfh). We explored the relation that exists PRDM1 response associated with an abnormal increased between the differentiation arrest of follicular lymphoma cells enrichment of the BCL6 protein repressor in PRDM1 gene. and loss-of-function of CREBBP acetyltransferase. Moreover, in these follicular lymphoma cells, pan-HDAC Experimental Design: The study used human primary cells inhibitor, vorinostat, restored their PRDM1 response to IL21 obtained from either follicular lymphoma tumors character- by lowering BCL6 bound to PRDM1. This finding was rein- ized for somatic mutations, or inflamed tonsils for normal forced by our exploration of patients with follicular lympho- germinal center B cells. Transcriptome and functional analyses ma treated with another pan-HDAC inhibitor. Patients were done to decipher the B- and T-cell crosstalk. Responses showed an increase of plasma cell identity genes, mainly were assessed by flow cytometry and molecular biology PRDM1 and XBP1, which underline the progression of follic- including ChIP-qPCR approaches. ular lymphoma B cells in the differentiation process. Results: Conversely to normal B cells, follicular lymphoma Conclusions: Our data uncover a new mechanism by which cells are unable to upregulate the transcription repressor, pan-HDAC inhibitors may act positively to treat patients with PRDM1, required for plasma cell differentiation. This defect follicular lymphoma through the induction of the expression occurs although the follicular lymphoma microenvironment of plasma cell genes. the microenvironment including IL21. This latter is a potent Introduction þ inducer of PCs and is produced by GC CD4 helper T cells called Adaptive immune responses involve the formation of germinal follicular helper T cells (Tfh; ref. 1). Akin to most cytokines, IL21 centers (GC), which are specialized structures allowing the dif- activates mainly JAK3/STAT3 signaling pathways (2), which are ferentiation of high-affinity B cells into long-lived memory B cells known to play a highly selective role in PC differentiation (3) and and plasma cells (PC). PCs are generated through specific tran- apoptosis, depending on the context (4). In humans, blockade of scriptional programs influenced by numerous signals delivered by IL21 inhibits PC generation. Tfh interacting with IL21 receptor expressing GC B cells could thus impact B-cell destiny via the 1Universite de Rennes, Etablissement Francais¸ du Sang (EFS) de Bretagne, Inserm, MICMAC–UMR_S1236, Rennes, France. 2Laboratoire d'hematologie, delivery of IL21 signal (3, 5). Human PCs emerging through Centre Hospitalier Universitaire de Rennes, Rennes, France. 3Laboratoire IL21/pSTAT3 signaling enhancement has concomitantly upregu- d'Anatomie Pathologique, Centre Hospitalier de Rennes, Rennes, France. lated PRDM1/BLIMP1 and downregulated BCL6 gene expression 4Gustave Roussy Cancer Campus, DITEP, Villejuif, France. 5Laboratoire de (6, 7). PRDM1/BLIMP1 is considered as the master PC factor that Cytogen etique, Centre Hospitalier de Rennes, Rennes, France. 6Service antagonizes BCL6 which sustains the B-cell identity (8, 9). There- 7 d'Hematologie Clinique, CH Bretagne Atlantique, Vannes, France. Service fore, PRDM1/BLIMP1 and BCL6 mutually inhibit each other and d'Hematologie Clinique, Centre Hospitalier de Rennes, Rennes, France. 8Inserm 9 BCL6-related inhibition of PC generation is predominantly relat- U918, Centre Henri Becquerel, Rouen, France. Laboratoire d'Immunologie, PRDM1 Centre Hospitalier de Rennes, Rennes, France. ed to BCL6 binding at intron 3 of where BCL6 recruits MTA3, which acts as a corepressor (10). Moreover, this PRDM1 Note: Supplementary data for this article are available at Clinical Cancer Research Online (http://clincancerres.aacrjournals.org/). intronic region contains an enhancer bound by CREBBP (11). CREBBP acts as a transcriptional coactivator of many different Current address for E. Guiheneuf: Laboratoire d'Hematologie, CHU Amiens- transcription factors through its intrinsic acetylation function on Picardie, Amiens, France. histone but also on nonhistone proteins including BCL6. Indeed, Corresponding Author: Thierry Fest, Rennes 1 University, 2 avenue du Pr Leon CREBBP binds and acetylates BCL6, leading to the inactivation of Bernard, Rennes 35043, France. Phone: 332-9928-4127; Fax: 332-9928-4152; its transcriptional repressor function (12, 13). E-mail: [email protected] In follicular lymphoma, we demonstrated earlier that the doi: 10.1158/1078-0432.CCR-18-1153 tumor microenvironment is enriched for Tfhs that sustain neo- Ó2018 American Association for Cancer Research. plastic cells (14, 15). It is generally accepted in the field that www.aacrjournals.org OF1 Downloaded from clincancerres.aacrjournals.org on September 24, 2021. © 2018 American Association for Cancer Research. Published OnlineFirst October 22, 2018; DOI: 10.1158/1078-0432.CCR-18-1153 Desmots et al. 2.0 Array and strategy for raw data normalization and filtering Translational Relevance is detailed in Supplementary Materials and Methods. In a number of phase I/II clinical trials, HDACi therapies have shown clinical responses, including complete remissions Gene expression analysis in previously multitreated patients with follicular lymphoma. The relative quantification of gene expression was determined ÀDDC Even if multiple biological effects have been described for using the 2 t method, then normalized to at least an internal these drugs, no effect on the master plasma cell regulator gene, control gene (ABL1, GAPDH, and/or HPRT1) and relative to a PRDM1, has been so far described in the context of the calibrator control sample corresponding to a mix of cDNA of follicular lymphoma. Indeed, our study revealed that follicular peripheral blood mononuclear cells (PBMC) from several healthy lymphomas with a CREBBP loss-of-function (concerns more donors. Statistical analysis using GraphPad Prism Software used than 50% of follicular lymphomas) were unable to upregulate Mann–Whitney test. PRDM1 expression despite the presence of IL21 in the tumor microenvironment, a potent inductor of PRDM1. In this Flow cytometry analysis, tissue immunostaining, Western context, we found that pan-HDACi can restore PRDM1 expres- blotting, and FISH analysis sion as well as other plasma cell genes, indicating a possible All antibodies and technical details concerning protein expres- reinitiation of follicular lymphoma B-cell differentiation. Our sion and FISH analyses are in Supplementary Materials and results highlight one effect of pan-HDACis to overcome fol- Methods, Supplementary Table S4, and Supplementary Figs. licular lymphomas' differentiation blockade. S3, S4, and S8. Culture conditions and molecular analyses Cells were cultured for 2 hours before receiving indicated follicular lymphomas are tumors reflective of centrocytes that fail treatments for 24 hours, and then CD19/CD20 viable B cells to differentiate beyond the GC exit point. Purified follicular were collected and used for subsequent DNA and RNA extraction. lymphoma B cells compared with normal GC B cells does not Detailed procedures are given in Supplementary Materials and identify a radically opposed signature, but rather substantial Methods. modification of normal GC expression driving most likely by numerous genetic and epigenetic somatic modifications Somatic mutations assessment (14, 16–18). Both histone acetyl transferases, CREBBP and EP300, We performed the SureSelect targeted-capture strategy from are commonly mutated in follicular lymphoma and CREBBP loss- Agilent Technologies using a panel of 34 genes described previ- of-function affects preferentially H3K27 acetylation whose deple- ously (22) with subsequent paired-end sequencing on an Illumina tion leads to downregulation of genes involved in GC output HiSeq 1500 Platform (Illumina). For variants detection, SureCall (19). Interestingly, the expression of these genes can be restored software from Agilent technologies was used (see Supplementary after inhibition of HDAC3 (20). Materials and Methods, Supplementary Tables S2B and S3B). In this study, we assessed PRDM1 gene expression and regu- lation to achieve new insights on the differentiation blockade that Statistical analyses characterizes the follicular lymphoma. We showed that despite a Statistical analyses were performed with the GraphPad Prism functional capacity to activate the IL21/pSTAT3 signaling, non- software V5 (GraphPad Software) as indicated in Supplementary functional CREBBP follicular lymphoma cells were unable to Materials and Methods section. increase PRDM1 expression. In these follicular
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