DOCSLIB.ORG

Gene Section Short Communication

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Gene Section Short Communication Atlas of Genetics and Cytogenetics in Oncology and Haematology OPEN ACCESS JOURNAL AT INIST-CNRS Gene Section Short Communication RASSF5 (Ras association (RalGDS/AF -6) domain family member 5) Lee Schmidt, Geoffrey J Clark University of Louisville, Room 119C, Baxter II Research Building, 580 S Preston Street, Louisville, KY 40202, USA (LS, GJC) Published in Atlas Database: October 2011 Online updated version : http://AtlasGeneticsOncology.org/Genes/RASSF5ID42059ch1q32.html DOI: 10.4267/2042/47279 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2012 Atlas of Genetics and Cytogenetics in Oncology and Haematology Identity DNA/RNA Other names: MGC10823, MGC17344, Maxp1, Description NORE1, NORE1A, NORE1B, RAPL, RASSF3 The human gene for RASSF5 is 81 kb in length and is HGNC (Hugo): RASSF5 located on chromosome 1(q32.1). The gene can Location: 1q32.1 produce 4 protein isoforms, two via differential exon usage, a third via differential promoter usage and the genesis of the 4 th (which can be found as an EST clone) is not yet clear. The largest isoform, A, is 418 amino acids long and has a molecular weight of about 47 kD. The protein structure of RASSF5A contains a proline- rich region at the N-terminus which contains potential SH3 binding sites and a nuclear localization signal. This is followed by a cystein rich domain, sometimes Note referred to as a zinc finger. Next is the Ras association Murine RASSF5 originally named Nore1a. Nore1B domain and this is followed by sequence containing the independently identified and designated RAPL. Rat SARAH motif required for binding to the pro-apoptotic RASSF5 also cloned independently and designated kinases MST1 and MST2. A second nuclear Maxp1. localization sequence has been reported between amino acids 200-260 and a nuclear export sequence between amino acids 260-300. Figure 1. Isoform A is shown as the longest isoform with 6 exons. Isoform B, without an alternate exon, shows that the frameshift gives a shortened and unique C-terminus. Isoform C is shown with a special 5' UTR and lacks an in-frame coding region leading to a unique N- terminus. The total coding sequence for Isoform A is about 1260 bases with the other isoforms being smaller. Atlas Genet Cytogenet Oncol Haematol. 2012; 16(3) 212 RASSF5 (Ras association (RalGDS/AF-6) domain family member 5) Schmidt L, Clark GJ Figure 2. A figure showing the processed mRNA as well as the amino acid sequence for isoforms A-D followed by motif explanation of isoform A (Nore1a). Atlas Genet Cytogenet Oncol Haematol. 2012; 16(3) 213 RASSF5 (Ras association (RalGDS/AF-6) domain family member 5) Schmidt L, Clark GJ Protein References Description Vavvas D, Li X, Avruch J, Zhang XF. Identification of Nore1 as a potential Ras effector. J Biol Chem. 1998 Mar The full length cDNA (for isoform A) encodes for a 47- 6;273(10):5439-42 kDa protein which contains a proline-rich region at the Khokhlatchev A, Rabizadeh S, Xavier R, Nedwidek M, Chen T, N-terminus followed by a putative Zhang XF, Seed B, Avruch J. Identification of a novel Ras- diacylglycerol/phorbol ester binding domain. This is regulated proapoptotic pathway. Curr Biol. 2002 Feb followed by the Ras association (RA) domain and then 19;12(4):253-65 the domain containing a SARAH motif. This later is Chen J, Lui WO, Vos MD, Clark GJ, Takahashi M, Schoumans responsible for binding to the pro-apoptotic kinases J, Khoo SK, Petillo D, Lavery T, Sugimura J, Astuti D, Zhang MST1 and MST2. C, Kagawa S, Maher ER, Larsson C, Alberts AS, Kanayama HO, Teh BT. The t(1;3) breakpoint-spanning genes LSAMP Expression and NORE1 are involved in clear cell renal cell carcinomas. Cancer Cell. 2003 Nov;4(5):405-13 Nore1a mRNA is expressed in the lung, kidney, liver, brain, spleen, thymus and heart. Hesson L, Dallol A, Minna JD, Maher ER, Latif F. NORE1A, a homologue of RASSF1A tumour suppressor gene is Localisation inactivated in human cancers. Oncogene. 2003 Feb 13;22(6):947-54 It can be detected on microtubules, in the centrosome, but appears most obvious in the nucleus. Vos MD, Martinez A, Ellis CA, Vallecorsa T, Clark GJ. The pro- apoptotic Ras effector Nore1 may serve as a Ras-regulated Function tumor suppressor in the lung. J Biol Chem. 2003 Jun 13;278(24):21938-43 RASSF5A is a pro-apoptotic Ras effector that can bind and relocalize the pro-apoptotic MST kinases in the Aoyama Y, Avruch J, Zhang XF. Nore1 inhibits tumor cell growth independent of Ras or the MST1/2 kinases. Oncogene. presence of activated Ras. It can also promote cell 2004 Apr 22;23(19):3426-33 cycle arrest and modulate the activity of p53 by Praskova M, Khoklatchev A, Ortiz-Vega S, Avruch J. regulating its' nuclear localization. Knockdown of Regulation of the MST1 kinase by autophosphorylation, by the RASSF5A promotes cellular proliferation and soft agar growth inhibitory proteins, RASSF1 and NORE1, and by Ras. growth. Thus, RASSF5A appears to function as a Ras Biochem J. 2004 Jul 15;381(Pt 2):453-62 regulated tumor suppressor. Analysis of human tumors Avruch J, Praskova M, Ortiz-Vega S, Liu M, Zhang XF. Nore1 has found little evidence of somatic mutation but the and RASSF1 regulation of cell proliferation and of the MST1/2 gene is frequently inactivated by promoter methylation kinases. Methods Enzymol. 2006;407:290-310 in a broad range of human tumors. RASSF5C (also Calvisi DF, Ladu S, Gorden A, Farina M, Conner EA, Lee JS, known as Nore1b or RAPL) has been reported to Factor VM, Thorgeirsson SS. Ubiquitous activation of Ras and modulate cellular adhesion and to be regulated by the Jak/Stat pathways in human HCC. Gastroenterology. 2006 Ras related protein Rap1a. RASSF5C has also been Apr;130(4):1117-28 implicated as serving as an adaptor protein to facilitate Donninger H, Vos MD, Clark GJ. The RASSF1A tumor the interaction of Ras and CARMA1. suppressor. J Cell Sci. 2007 Sep 15;120(Pt 18):3163-72 Kumari G, Singhal PK, Rao MR, Mahalingam S. Nuclear Mutations transport of Ras-associated tumor suppressor proteins: different transport receptor binding specificities for arginine-rich Note nuclear targeting signals. J Mol Biol. 2007 Apr 13;367(5):1294- 311 No tumor mutations yet reported. Calvisi DF, Donninger H, Vos MD, Birrer MJ, Gordon L, Leaner V, Clark GJ. NORE1A tumor suppressor candidate modulates Implicated in p21CIP1 via p53. Cancer Res. 2009 Jun 1;69(11):4629-37 Clear cell renal carcinoma Richter AM, Pfeifer GP, Dammann RH. The RASSF proteins in cancer; from epigenetic silencing to functional characterization. Note Biochim Biophys Acta. 2009 Dec;1796(2):114-28 RASSF5 is frequently down-regulated by promoter Bee C, Moshnikova A, Mellor CD, Molloy JE, Koryakina Y, methylation in a variety of tumors including clear cell Stieglitz B, Khokhlatchev A, Herrmann C. Growth and tumor renal carcinomas. Moreover, a rare hereditary form of suppressor NORE1A is a regulatory node between Ras kidney cancer has been reported that maps with a signaling and microtubule nucleation. J Biol Chem. 2010 May translocation inactivating the RASSF5 gene. 21;285(21):16258-66 Kumari G, Singhal PK, Suryaraja R, Mahalingam S. Functional Various cancers interaction of the Ras effector RASSF5 with the tyrosine kinase Note Lck: critical role in nucleocytoplasmic transport and cell cycle Nore1a is frequently inactivated by promoter regulation. J Mol Biol. 2010 Mar 19;397(1):89-109 methylation in renal carcinoma, breast cancer, lung Park J, Kang SI, Lee SY, Zhang XF, Kim MS, Beers LF, Lim cancer, liver cancer and neurological tumors. DS, Avruch J, Kim HS, Lee SB. Tumor suppressor ras Atlas Genet Cytogenet Oncol Haematol. 2012; 16(3) 214 RASSF5 (Ras association (RalGDS/AF-6) domain family member 5) Schmidt L, Clark GJ association domain family 5 (RASSF5/NORE1) mediates death This article should be referenced as such: receptor ligand-induced apoptosis. J Biol Chem. 2010 Nov 5;285(45):35029-38 Schmidt L, Clark GJ. RASSF5 (Ras association (RalGDS/AF- 6) domain family member 5). Atlas Genet Cytogenet Oncol Overmeyer JH, Maltese WA. Death pathways triggered by Haematol. 2012; 16(3):212-215. activated Ras in cancer cells. Front Biosci. 2011 Jan 1;16:1693-713 Atlas Genet Cytogenet Oncol Haematol. 2012; 16(3) 215 .
Load more

Recommended publications
  • Supplemental Table 1. Complete Gene Lists and GO Terms from Figure 3C
    Supplemental Table 1. Complete gene lists and GO terms from Figure 3C. Path 1 Genes: RP11-34P13.15, RP4-758J18.10, VWA1, CHD5, AZIN2, FOXO6, RP11-403I13.8, ARHGAP30, RGS4, LRRN2, RASSF5, SERTAD4, GJC2, RHOU, REEP1, FOXI3, SH3RF3, COL4A4, ZDHHC23, FGFR3, PPP2R2C, CTD-2031P19.4, RNF182, GRM4, PRR15, DGKI, CHMP4C, CALB1, SPAG1, KLF4, ENG, RET, GDF10, ADAMTS14, SPOCK2, MBL1P, ADAM8, LRP4-AS1, CARNS1, DGAT2, CRYAB, AP000783.1, OPCML, PLEKHG6, GDF3, EMP1, RASSF9, FAM101A, STON2, GREM1, ACTC1, CORO2B, FURIN, WFIKKN1, BAIAP3, TMC5, HS3ST4, ZFHX3, NLRP1, RASD1, CACNG4, EMILIN2, L3MBTL4, KLHL14, HMSD, RP11-849I19.1, SALL3, GADD45B, KANK3, CTC- 526N19.1, ZNF888, MMP9, BMP7, PIK3IP1, MCHR1, SYTL5, CAMK2N1, PINK1, ID3, PTPRU, MANEAL, MCOLN3, LRRC8C, NTNG1, KCNC4, RP11, 430C7.5, C1orf95, ID2-AS1, ID2, GDF7, KCNG3, RGPD8, PSD4, CCDC74B, BMPR2, KAT2B, LINC00693, ZNF654, FILIP1L, SH3TC1, CPEB2, NPFFR2, TRPC3, RP11-752L20.3, FAM198B, TLL1, CDH9, PDZD2, CHSY3, GALNT10, FOXQ1, ATXN1, ID4, COL11A2, CNR1, GTF2IP4, FZD1, PAX5, RP11-35N6.1, UNC5B, NKX1-2, FAM196A, EBF3, PRRG4, LRP4, SYT7, PLBD1, GRASP, ALX1, HIP1R, LPAR6, SLITRK6, C16orf89, RP11-491F9.1, MMP2, B3GNT9, NXPH3, TNRC6C-AS1, LDLRAD4, NOL4, SMAD7, HCN2, PDE4A, KANK2, SAMD1, EXOC3L2, IL11, EMILIN3, KCNB1, DOK5, EEF1A2, A4GALT, ADGRG2, ELF4, ABCD1 Term Count % PValue Genes regulation of pathway-restricted GDF3, SMAD7, GDF7, BMPR2, GDF10, GREM1, BMP7, LDLRAD4, SMAD protein phosphorylation 9 6.34 1.31E-08 ENG pathway-restricted SMAD protein GDF3, SMAD7, GDF7, BMPR2, GDF10, GREM1, BMP7, LDLRAD4, phosphorylation
    [Show full text]
  • Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5
    Structure Article Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5 Lisheng Ni,1 Sheng Li,1,4 Jianzhong Yu,3 Jungki Min,1,5 Chad A. Brautigam,2 Diana R. Tomchick,2 Duojia Pan,3 and Xuelian Luo1,* 1Department of Pharmacology 2Department of Biophysics The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA 3Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA 4Present address: Biologics Department, Shanghai ChemPartner Co. Ltd., Shanghai 201203, China 5Present address: Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710, USA *Correspondence: [email protected] http://dx.doi.org/10.1016/j.str.2013.07.008 SUMMARY dor (Sav1), the NDR family kinases Lats1 and Lats2 (Lats1/2), and the adaptor protein Mob1. They form a central kinase The tumor-suppressive Hippo pathway controls cascade to transduce signals from cell-surface receptors tissue homeostasis through balancing cell prolifera- (Avruch et al., 2012; Hergovich, 2012). tion and apoptosis. Activation of the kinases Mst1 In the canonical Hippo kinase cascade, Mst1/2, in complex and Mst2 (Mst1/2) is a key upstream event in this with Sav1, phosphorylate and activate the Lats1/2-Mob1 com- pathway and remains poorly understood. Mst1/2 plexes, which then phosphorylate the transcriptional coactivator and their critical regulators RASSFs contain Yes-associated protein (YAP), a major downstream target of the Hippo pathway (Dong et al., 2007; Hao et al., 2008; Hong and Salvador/RASSF1A/Hippo (SARAH) domains that Guan, 2012; Huang et al., 2005; Zhao et al., 2007).
    [Show full text]
  • STRIPAK Complexes in Cell Signaling and Cancer
    Oncogene (2016), 1–9 © 2016 Macmillan Publishers Limited All rights reserved 0950-9232/16 www.nature.com/onc REVIEW STRIPAK complexes in cell signaling and cancer Z Shi1,2, S Jiao1 and Z Zhou1,3 Striatin-interacting phosphatase and kinase (STRIPAK) complexes are striatin-centered multicomponent supramolecular structures containing both kinases and phosphatases. STRIPAK complexes are evolutionarily conserved and have critical roles in protein (de) phosphorylation. Recent studies indicate that STRIPAK complexes are emerging mediators and regulators of multiple vital signaling pathways including Hippo, MAPK (mitogen-activated protein kinase), nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are extensively involved in a variety of fundamental biological processes ranging from cell growth, differentiation, proliferation and apoptosis to metabolism, immune regulation and tumorigenesis. Growing evidence correlates dysregulation of STRIPAK complexes with human diseases including cancer. In this review, we summarize the current understanding of the assembly and functions of STRIPAK complexes, with a special focus on cell signaling and cancer. Oncogene advance online publication, 15 February 2016; doi:10.1038/onc.2016.9 INTRODUCTION in the central nervous system and STRN4 is mostly abundant in Recent proteomic studies identified a group of novel multi- the brain and lung, whereas STRN3 is ubiquitously expressed in 5–9 component complexes named striatin (STRN)-interacting phos- almost all tissues. STRNs share a
    [Show full text]
  • The Discovery of a Novel, Ras-Mediated NORE1A/PMLIV Tumor Suppressor Complex." (2016)
    University of Louisville ThinkIR: The University of Louisville's Institutional Repository Electronic Theses and Dissertations 8-2016 The discovery of a novel, Ras-mediated NORE1A/ PMLIV tumor suppressor complex. Jessica Mezzanotte Sharpe University of Louisville Follow this and additional works at: https://ir.library.louisville.edu/etd Part of the Medical Biochemistry Commons, and the Medical Molecular Biology Commons Recommended Citation Sharpe, Jessica Mezzanotte, "The discovery of a novel, Ras-mediated NORE1A/PMLIV tumor suppressor complex." (2016). Electronic Theses and Dissertations. Paper 2539. https://doi.org/10.18297/etd/2539 This Doctoral Dissertation is brought to you for free and open access by ThinkIR: The nivU ersity of Louisville's Institutional Repository. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of ThinkIR: The nivU ersity of Louisville's Institutional Repository. This title appears here courtesy of the author, who has retained all other copyrights. For more information, please contact [email protected]. THE DISCOVERY OF A NOVEL, RAS-MEDIATED NORE1A/PMLIV TUMOR SUPPRESSOR COMPLEX By: Jessica Mezzanotte Sharpe B.A., Vanderbilt University, 2010 M.S., University of Louisville, 2014 A Dissertation Submitted to the Faculty of the School of Medicine of the University of Louisville in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Biochemistry and Molecular Biology Department of Biochemistry and Molecular Genetics University of Louisville Louisville, KY August, 2016 Copyright 2016 by Jessica Mezzanotte Sharpe All Rights Reserved THE DISCOVERY OF A NOVEL, RAS-MEDIATED NORE1A/PMLIV TUMOR SUPPRESSOR COMPLEX By Jessica Mezzanotte Sharpe B.A., Vanderbilt University, 2010 M.S., University of Louisville, 2014 A Dissertation Approved on June 8, 2016 by the following Dissertation Committee: __________________________________________ Dr.
    [Show full text]
  • RASSF6; the Putative Tumor Suppressor of the RASSF Family
    Review RASSF6; the Putative Tumor Suppressor of the RASSF Family Hiroaki Iwasa 1, Xinliang Jiang 2 and Yutaka Hata 1,2,* Received: 2 November 2015; Accepted: 1 December 2015; Published: 9 December 2015 Academic Editor: Reinhard Dammann 1 Department of Medical Biochemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8510, Japan; [email protected] 2 Center for Brain Integration Research, Tokyo Medical and Dental University, Tokyo 113-8510, Japan; [email protected] * Correspondence: [email protected]; Tel.: +81-3-5803-5164; Fax: +81-3-5803-0121 Abstract: Humans have 10 genes that belong to the Ras association (RA) domain family (RASSF). Among them, RASSF7 to RASSF10 have the RA domain in the N-terminal region and are called the N-RASSF proteins. In contradistinction to them, RASSF1 to RASSF6 are referred to as the C-RASSF proteins. The C-RASSF proteins have the RA domain in the middle region and the Salvador/RASSF/Hippo domain in the C-terminal region. RASSF6 additionally harbors the PSD-95/Discs large/ZO-1 (PDZ)-binding motif. Expression of RASSF6 is epigenetically suppressed in human cancers and is generally regarded as a tumor suppressor. RASSF6 induces caspase-dependent and -independent apoptosis. RASSF6 interacts with mammalian Ste20-like kinases (homologs of Drosophila Hippo) and cross-talks with the Hippo pathway. RASSF6 binds MDM2 and regulates p53 expression. The interactions with Ras and Modulator of apoptosis 1 (MOAP1) are also suggested by heterologous protein-protein interaction experiments. RASSF6 regulates apoptosis and cell cycle through these protein-protein interactions, and is implicated in the NF-κB and JNK signaling pathways.
    [Show full text]
  • Differences in Gene Expression Profiles and Carcinogenesis Pathways Involved in Cisplatin Resistance of Four Types of Cancer
    596 ONCOLOGY REPORTS 30: 596-614, 2013 Differences in gene expression profiles and carcinogenesis pathways involved in cisplatin resistance of four types of cancer YONG YANG1,2, HUI LI1,2, SHENGCAI HOU1,2, BIN HU1,2, JIE LIU1,3 and JUN WANG1,3 1Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Capital Medical University, Beijing 100069; 2Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020; 3Department of Physiology, Capital Medical University, Beijing 100069, P.R. China Received December 23, 2012; Accepted March 4, 2013 DOI: 10.3892/or.2013.2514 Abstract. Cisplatin-based chemotherapy is the standard Introduction therapy used for the treatment of several types of cancer. However, its efficacy is largely limited by the acquired drug Cisplatin is primarily effective through DNA damage and is resistance. To date, little is known about the RNA expression widely used for the treatment of several types of cancer, such changes in cisplatin-resistant cancers. Identification of the as testicular, lung and ovarian cancer. However, the ability RNAs related to cisplatin resistance may provide specific of cancer cells to become resistant to cisplatin remains a insight into cancer therapy. In the present study, expression significant impediment to successful chemotherapy. Although profiling of 7 cancer cell lines was performed using oligo- previous studies have identified numerous mechanisms in nucleotide microarray analysis data obtained from the GEO cisplatin resistance, it remains a major problem that severely database. Bioinformatic analyses such as the Gene Ontology limits the usefulness of this chemotherapeutic agent. Therefore, (GO) and KEGG pathway were used to identify genes and it is crucial to examine more elaborate mechanisms of cisplatin pathways specifically associated with cisplatin resistance.
    [Show full text]
  • Genome-Wide Association Study of Growth Performance and Immune Response to Newcastle Disease Virus of Indigenous Chicken in Rwanda
    ORIGINAL RESEARCH published: 16 August 2021 doi: 10.3389/fgene.2021.723980 Genome-Wide Association Study of Growth Performance and Immune Response to Newcastle Disease Virus of Indigenous Chicken in Rwanda Richard Habimana 1,2*, Kiplangat Ngeno 2, Tobias Otieno Okeno 2, Claire D’ andre Hirwa 3, Christian Keambou Tiambo 4 and Nasser Kouadio Yao 5 1 College of Agriculture, Animal Science and Veterinary Medicine, University of Rwanda, Kigali, Rwanda, 2 Animal Breeding and Genomics Group, Department of Animal Science, Egerton University, Egerton, Kenya, 3 Rwanda Agricultural and Animal Resources Development Board, Kigali, Rwanda, 4 Centre for Tropical Livestock Genetics and Health, International Livestock Research Institute, Nairobi, Kenya, 5 Biosciences Eastern and Central Africa – International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya A chicken genome has several regions with quantitative trait loci (QTLs). However, Edited by: replication and confirmation of QTL effects are required particularly in African chicken Younes Miar, Dalhousie University, populations. This study identified single nucleotide polymorphisms (SNPs) and putative Canada genes responsible for body weight (BW) and antibody response (AbR) to Newcastle Reviewed by: disease (ND) in Rwanda indigenous chicken (IC) using genome-wide association studies Sayed Haidar Abbas, Northwest A & F University, China (GWAS). Multiple testing was corrected using chromosomal false detection rates of 5 and Suxu Tan, 10% for significant and suggestive thresholds, respectively. BioMart data mining and Michigan State University, variant effect predictor tools were used to annotate SNPs and candidate genes, respectively. United States A total of four significant SNPs (rs74098018, rs13792572, rs314702374, and rs14123335) *Correspondence: Richard Habimana significantly (p ≤ 7.6E−5) associated with BW were identified on chromosomes (CHRs) [email protected] 8, 11, and 19.
    [Show full text]
  • Omaima Nasir1
    Available online freely at www.isisn.org Bioscience Research Print ISSN: 1811-9506 Online ISSN: 2218-3973 Journal by Innovative Scientific Information & Services Network RESEARCH ARTICLE BIOSCIENCE RESEARCH, 2020 17(1): 327-346. OPEN ACCESS Exploring the Physiological and Molecular Mechanism of the Gum arabic ( Acacia senegal ) Based on Gene Expression Microarray Omaima Nasir1 1Department of Biology, Turabah University College, Taif University, Saudi Arabia. *Correspondence: [email protected], [email protected] Received 22-01-2019, Revised: 16-02-2020, Accepted: 20- 02-2020 e-Published: 01-03-2020 To investigate the differential expression of genes after Gum Arabic (Acacia senegal) administration by microarray study.The microarray was performed using colonic tissue of BALB/c s at 8 weeks of age treated with10% (w/w) GA dissolved in drinking water and control had a normal tap water for 4 days. A total 100 genes were analyzed by the pathway, gene ontology (GO) enrichment analysis, construction of protein-protein interaction (PPI) network, module analysis, construction of target genes—miRNA interaction network, target and genes-transcription factor (TF) interaction. We discuss key issues pertaining to experimental design, data preprocessing, and gene selection methods. Common types of data representation are illustrated. A total of 100 differentially miRNAs were screened and identified by microarray according to fold change the top genes which were significantly 59 genes with up-regulation and 41 genes down regulation, after Gum Arabic administration for 4 days. The data may unravel the future molecular mechanisms of Gum arabic by applying various bio-informatical approaches, we have revealed top score upregulation genes were 23, and down regulated genes with top score were 38.
    [Show full text]
  • Receptor Signaling Through Osteoclast-Associated Monocyte
    Downloaded from http://www.jimmunol.org/ by guest on September 29, 2021 is online at: average * The Journal of Immunology The Journal of Immunology , 20 of which you can access for free at: 2015; 194:3169-3179; Prepublished online 27 from submission to initial decision 4 weeks from acceptance to publication February 2015; doi: 10.4049/jimmunol.1402800 http://www.jimmunol.org/content/194/7/3169 Collagen Induces Maturation of Human Monocyte-Derived Dendritic Cells by Signaling through Osteoclast-Associated Receptor Heidi S. Schultz, Louise M. Nitze, Louise H. Zeuthen, Pernille Keller, Albrecht Gruhler, Jesper Pass, Jianhe Chen, Li Guo, Andrew J. Fleetwood, John A. Hamilton, Martin W. Berchtold and Svetlana Panina J Immunol cites 43 articles Submit online. Every submission reviewed by practicing scientists ? is published twice each month by Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Author Choice option Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts http://jimmunol.org/subscription Freely available online through http://www.jimmunol.org/content/suppl/2015/02/27/jimmunol.140280 0.DCSupplemental This article http://www.jimmunol.org/content/194/7/3169.full#ref-list-1 Information about subscribing to The JI No Triage! Fast Publication! Rapid Reviews! 30 days* Why • • • Material References Permissions Email Alerts Subscription Author Choice Supplementary The Journal of Immunology The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2015 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606.
    [Show full text]
  • Rassf Family of Tumor Suppressor Polypeptides
    Rassf Family of Tumor Suppressor Polypeptides The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Avruch, Joseph, Ramnik Xavier, Nabeel Bardeesy, Xian-feng Zhang, Maria Praskova, Dawang Zhou, and Fan Xia. 2008. “Rassf Family of Tumor Suppressor Polypeptides.” Journal of Biological Chemistry 284 (17): 11001–5. https://doi.org/10.1074/jbc.r800073200. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483004 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA MINIREVIEW This paper is available online at www.jbc.org THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 284, NO. 17, pp. 11001–11005, April 24, 2009 © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in the U.S.A. Rassf Family of Tumor Rassf1A expression inhibits proliferation and tumor growth in * nude mice. Most persuasively, specific knock-outs of the exon Suppressor Polypeptides encoding the unique N terminus of Rassf1A result in increased Published, JBC Papers in Press, December 17, 2008, DOI 10.1074/jbc.R800073200 numbers of tumors in older mice, specifically lymphomas, lung ‡§¶1 ¶ʈ ¶‡‡ Joseph Avruch , Ramnik Xavier **, Nabeel Bardeesy , tumors, and gastrointestinal tumors (7, 8); increased numbers Xian-feng Zhang‡§¶, Maria Praskova‡§¶, Dawang Zhou‡§¶, and
    [Show full text]
  • NORE1 (RASSF5) Mouse Monoclonal Antibody [Clone ID: OTI1C6] Product Data
    OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA502434 NORE1 (RASSF5) Mouse Monoclonal Antibody [Clone ID: OTI1C6] Product data: Product Type: Primary Antibodies Clone Name: OTI1C6 Applications: WB Recommend Dilution: WB 1:500 Reactivity: Human Host: Mouse Isotype: IgG2b Clonality: Monoclonal Immunogen: Full length human recombinant protein of human RASSF5 (NP_872606) produced in HEK293T cell. Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Concentration: 1 mg/ml Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) Predicted Protein Size: 30.2 kDa Gene Name: Ras association domain family member 5 Database Link: NP_872606 Entrez Gene 83593 Human Background: This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Synonyms: Maxp1; NORE1; NORE1A; NORE1B; RAPL; RASSF3 Protein Families: Druggable Genome Protein Pathways: Leukocyte transendothelial migration, Non-small cell lung cancer, Pathways in cancer This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2020 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 NORE1 (RASSF5) Mouse Monoclonal Antibody [Clone ID: OTI1C6] – TA502434 Product images: HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY RASSF5 ([RC203854], Right lane) cDNA for 48 hrs and lysed.
    [Show full text]
  • Analysis of DNA Methylation Sites Used for Forensic Age Prediction and Their Correlation with Human Aging
    Silva DSBS, et al., J Forensic Leg Investig Sci 2021, 7: 054 DOI: 10.24966/FLIS-733X/100054 HSOA Journal of Forensic, Legal & Investigative Sciences Research Article Analysis of DNA Methylation Introduction DNA samples left behind in crime scenes are often used for Sites used for Forensic Age identification purposes by comparing them to reference samples or to a forensic database. In cases where there is no match, investigators may Prediction and their Correlation turn to alternative approaches using advanced technologies to gain valuable leads on phenotypic traits (externally visible characteristics) with Human Aging of the person who had left the DNA material. Previous studies have found that phenotypes can be predicted by using information obtained from different DNA markers, such as single nucleotide Silva DSBS1* and Karantenislis G2 polymorphisms (SNPs), insertion/deletion polymorphisms (InDel), and epigenetic markers [1-11]. It is well established that epigenetic 1Department of Chemistry, Hofstra University, Hempstead-NY, USA modifications are major regulators in the translation of genotype 2John F Kennedy High School, Bellmore-NY, USA to phenotype. Epigenetic regulation encompasses various levels of gene expression and involves modifications of DNA, histones, RNA and chromatin, with functional consequences for the human Abstract genome [12-14]. DNA methylation is the most well-characterized epigenetic modification. It is usually associated with transcriptional Phenotype-related features, such as age, are linked to repression and it involves the addition of a methyl group (– CH3) in genetic components through regulatory pathways and epigenetic the cytosine of CpG dinucleotides. Differences in DNA methylation modifications, such as DNA methylation, are major regulators in levels in specific regions of the genome can affect the expression of the translation of genotype to phenotype.
    [Show full text]