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Sasiwimol Kaewkamol Forest Biological Science Forest Biology, Forestry Advisors: Dr.Nisa Leksungnoen and Assoc

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Sasiwimol Kaewkamol Forest Biological Science Forest Biology, Forestry Advisors: Dr.Nisa Leksungnoen and Assoc Presentation by Sasiwimol Kaewkamol Forest Biological Science Forest Biology, Forestry Advisors: Dr.Nisa Leksungnoen and Assoc. Prof. Dr. Duangchai Sookchaloem Kasetsart University Chlorophyll and stomatal comparison in Typhonium trilobatum (L.) schott from 6 regions in Thailand Presentation 1 Outline Introduction 2 Objectives 3 Site study 4 7 6 5 Materials & Results & Recomendation Conclusion Method Discussion 1/34 1 Introduction Classification Phylum Class Order Tracheophyta Liliopsida Alismatales Species Genus Family Typhonium trilobatum Typhonium Araceae (L.) schott 2/34 1 Trilobe leaf Introduction Wavy margin Morphology Leaf Flower with vermilion red Spathe Petiole is 25-40 cm. ศูนยพัฒนาการแพทยแผนไทย (2547); สํานักวิจัยการอนุรักษปาไมและพันพืชธุ (2556) 3/34 Found In: 1 Assam, Bangladesh, Cambodia, Introduction China South-Central, Sookchaloem (1995); Sookchaloem and Murata (1997); China Southeast, Hainan, Govaerts and G.Frodin (2002); Boyce et al. (2012) India, Laos, Malaya, Myanmar,Nepal, Distribution Sri Lanka, Thailand, Vietnam Introduced into: Borneo, Ivory Coast, Philippines, Trinidad- Tobago, Windward Is. Native Introduced Picture form: http://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:89268-1 4/34 1 Introduction Distribution inThailand Sookchaloem (1995) 5/34 1 Introduction Photosynthesis 6/34 1 Introduction Medicine Benefits Shahriar et al. (2015) Bown (2000) 7/34 2 Objectives To study and compare the Performance index , Chlorophyll content and Stomatal density in Typhonium trilobatum (L.) schott of 6 provinces in each region of Thailand. 8/34 3 Site study Field study At Central Botanical Garden (Pukae), Chaloem-Phra-Kiat District, Saraburi 9/34 3 Site study Laboratory Laboratory of Faculty of Forestry, Kasetsart University 10/34 4 Materials & Methods 11/34 4 Materials & Methods 12/34 4 Materials & Methods Study peroids September-November 2560 13/34 4 Materials & Methods Study Typhonium trilobatum (L.) schott Phichit Chaing Rai Nong Khai Chanthaburi Phetchaburi Nakhon Si Thammarat 14/34 4 Materials & Methods Study Typhonium trilobatum (L.) schott Planted on June 2560 22 cm. with Clay loam Age ~ 3 month 20 cm. 5 pot per province 15/34 4 Materials & Methods Field Collecting the Performance index (PI) study Chlorophyll Fluorometer 16/34 4 Materials & Methods Field Collecting the Performance index (PI) study 2 leaf per pot Hold the clips for 30 minutes Performance index (PI) Collecting every the early of month and the late of month in study period 17/34 4 Materials & Methods Field Collecting the Chlorophyll content study Digital Chlorophyll meter (SPAD) 18/34 4 Materials & Methods Field Collecting the Chlorophyll content study 10 .. 1 2 3 Digital Chlorophyll meter (SPAD) Repeat 10 times per 1 leaf 19/34 4 Materials & Method Field Leaf sample for stomatal study study Cutting the leaf Size 1X1 cm. 3 collecting per leaf 1 cm. 1 cm. Adapted form ประศาสตร์ (2551) 20/34 4 Materials & Method Field Leaf sample for stomatal study study The leaf sample are arranged according to the province. จังหวัดเชียงราย Formalin Separate the leaf into the ziplock Adapted form ประศาสตร์ (2551) 21/34 4 Materials & Methods Laboratory Stomatal study About 10 minutes Water Keep it cool Soak in water for at least 2 days. Adapted form ประศาสตร์ (2551) 22/34 4 Materials & Method Stomatal study Laboratory Safranin O About 10 minutes Adapted form ประศาสตร์ (2551) 23/34 4 Materials & Method Laboratory Stomatal study x Random size measurement the stomatal 10 stomata under power objective of 40X40 24/34 4 Materials & Method Stomatal study Laboratory 1 mm. 1 Repeat 3 times mm. Count the stomatal and epidermal cell at 1X1 millimeter under power objective of 10X10 25/34 4 Materials & Method Statistical Analysis ANOVA (analysis of varience) One – Way ANOVA 1 factor Typhonium trilobatum Chaing Rai (L.) schott Nong Khai Phichit 6 level Provinces Chanthaburi Phetchaburi Nakhon Si Thammarat 5 replicates Pot + + + + LSD (Least Square Difference) Method were employed to find the statistical difference at the significant level of 0.05 26/34 5 Results &Discussion 1. Chlorophyll 1.1 Performance index (PI) Živčák et al. (2014); Franić et al. (2015) Performance index (PI) a a ab ab b b 6.15 6.07 5.69 5.6 5.27 5.04 27/34 5 Results &Discussion 1. Chlorophyll 1.2 Chlorophyll content Brand (1997); Argenta et al. (2004) Chlorophyll content (SPAD) a bc b bc c c 41.6 35.78 34.65 33.93 33.58 32.9 28/34 5 Results &Discussion 2. Stomatal Amphistomatous leaf 0.02 mm. Zade (2016) 0 . 031 mm. Size average Anomocytic Staurocytic เทียมใจ (2542) 29/34 5 Results &Discussion 2. Stomatal Meidner and Mansfield (1968) 2.1 Stomatal density Stomatal Density a (No.stomata/millimeter square) a a b b ab b b bc c 157.92 153.82 c c 129.87 124.53 118.83 107.33 142 124.36 a ab 107 101.80 98.08 bc c ab bc 90.22 15.92 29.45 22.87 22.73 20.75 23.58 Uppper surface Lower surface Total 30/34 5 Results &Discussion 1. Chlorophyll 2.1 Stomatal density Total stomatal density a a b b Tanaka et al. (2013) b b 157.92 153.82 129.87 124.53 118.83 107.33 31/34 5 Results &Discussion Overview a a 157.92 b b 153.82 b b 129.87 124.53 118.83 107.33 a bc c c bc b 41.6 33.93 32.9 33.58 34.65 35.78 ab b b a a ab 5.6 5.27 5.04 6.07 6.15 5.69 Performance index (PI) Chlrophyll content (SPAD) Total stomata density 32/34 6 Conclusion Typhonium trilobatum (L.) schott form Chiang Rai province is recommended to propagate for medicinal and economic values. 33/34 7 Recomendation 1. Study in the real habitat of Triphonium trilobatum (L.) schott in each areas. 2. Study other factors related to plant growth 34/34 THANK YOU Advisors: Dr.Nisa Leksungnoen and Assoc. Prof. Dr. Duangchai Sookchaloem Mr. Guson Tangchaiphitak Central Botanical Garden (Pukae) Team Mr. Sgreen Norith Forestry 80 Friend My family Performance index Chlrophyll content Total stomata Provinces SD SD SD (PI) (SPAD) density Chiang Rai 5.6 1.18 41.6 4.16 157.92 25.45 Phetchaburi 5.27 1.23 33.93 5.67 153.82 23.57 Nakhon Si Thammarat 5.04 1.38 32.9 4.91 129.87 29.81 Nongkhai 6.07 1.33 33.58 3.65 124.53 19.39 Chanthaburi 6.15 1.56 34.65 3.98 118.83 39.72 Phichit 5.69 1.33 35.78 4.07 107.33 39.60 จังหวัด ความกดอากาศ (hPa) อุณหภูมิ (°C) ความชื้นสัมพัทธ (%) ปริมาณน้ําฝน (มม.) การคายระเหยน้ํา (มม.) เชียงราย 1009.28 24.6 76.1 1707.4 1350 พิจิตร 1009 33.3 77.6 1269 1538 นครศรีธรรมราช 1009.7 32.5 81.7 2603 1426.1 จันทบุรี 1009.5 32.4 78.3 2997.5 1499.5 เพชรบุรี 1009.4 32.2 76.2 977.1 1536.6 หนองคาย 1009.62 26.6 74.2 1632.5 1478.8 สระบุรี* 1009 29 71 1300 152 ขอมูลจาก สถิติภูมิอาศยอนหลัง พ.ศ.2529-2559 กรมอุตุนิยมวิทยา * จังหวัดสระบุรีไมมีสถานีตรวจวัดสภาพภูมิอากาศ จึงใชคาสถิติภูมิอากาศจากจังหวัดลพบุรีแทน.
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