DOCSLIB.ORG

Porphyrin-Lipid Nanovesicles (Porphysomes) Are Effective

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Porphyrin-Lipid Nanovesicles (Porphysomes) Are Effective Nanophotonics 2021; 10(12): 3161–3168 Research Article Keegan Guidolin, Lili Ding, Juan Chen, Brian C. Wilson and Gang Zheng* Porphyrin-lipid nanovesicles (Porphysomes) are effective photosensitizers for photodynamic therapy https://doi.org/10.1515/nanoph-2021-0220 and complete tumor response rates (15 vs. 25%, p = 0.52). Received May 6, 2021; accepted June 9, 2021; Hence, porphysome nanoparticles are an effective PDT published online June 22, 2021 agent and have the additional advantages of multimodal diagnostic and therapeutic applications arising from their Abstract: Porphysomes (PS) are liposome-like nano- intrinsic structure. Porphysomes may also be the first particles comprising pyropheophorbide-conjugated single all-organic agent capable of concurrent PDT phospholipids that have demonstrated potential as and PTT. multimodal theranostic agents for applications that include phototherapies, targeted drug delivery and in vivo Keywords: cancer; nanomedicine; nanoparticles; photo- fluorescence, photoacoustic, magnetic resonance or dynamic therapy; photofrin; porphysome. positron emission imaging. Previous therapeutic appli- cations focused primarily on photothermal therapy (PTT) and suggested that PSs require target-triggered activation 1 Introduction for use as photodynamic therapy (PDT) sensitizers. Here, athymic nude mice bearing subcutaneous A549 human Porphysomes (PSs) are nanoparticles (∼100 nm) composed lung tumors were randomized into treatment and control of pyropheophorbide-conjugated phospholipid (pyro-lipid) groups: PS-PDT at various doses, PS-only and no treat- subunits that self-assemble into liposome-like structures, ment negative controls, as well as positive controls using each containing ∼80,000 porphyrin molecules [1]. They are the clinical photosensitizer Photofrin. Animals were fol- preferentially taken up and retained in solid tumors via the lowed for 30 days post-treatment. PS-PDT at all doses enhanced permeability and retention (EPR) effect and sub- demonstrated a significant tumor ablative effect, with the sequently taken up by cancer cells and disrupted into pyro- greatest effect seen with 10 mg/kg PS at a drug-light in- lipid subunits [1]. Previous studies have suggested that the terval of 24 h. By comparison, negative controls (PS-only, PSs can be exploited in both their structurally intact state Photofrin-only, and no treatment) showed uncontrolled (i.e., as nanoparticles) and in their disrupted state (i.e., as tumor growth. PDT with Photofrin at 5 mg/kg and PS at pyro-lipid subunits). In the former state, absorption of 10 mg/kg demonstrated similar tumor growth suppression light energy is dissipated through thermal conversion and no significant fluorescence emission or photochemistry occurs due to the tight packing of porphyrin molecules in the porphyrin-lipid bilayer, resulting in a quenching of *Corresponding author: Gang Zheng, Department of Surgery, >99% [1–7]. Upon disruption, the pyro-lipid subunits are University of Toronto, Toronto, ON, Canada; and Department of unquenched, enabling fluorescence and photochemical Medical Biophysics, University of Toronto, Toronto, ON, Canada, reactions [2, 4, 7, 8]. These previous studies suggest that the E-mail: [email protected] conversion of PSs from their intact to their disrupted state is Keegan Guidolin, Department of Surgery, University of Toronto, Toronto, ON, Canada; Princess Margaret Cancer Centre, Toronto, ON, time dependent, though the relative populations of intact Canada; and Institute of Biomedical Engineering, University of and disrupted PSs at any given time point are not well Toronto, Toronto, ON, Canada. https://orcid.org/0000-0001-6482- characterized. 8024 PSs in various formulations have been shown to be Lili Ding and Juan Chen, Princess Margaret Cancer Centre, Toronto, effective multimodality agents for theranostic applications, ON, Canada including in vivo photoacoustic imaging and photothermal Brian C. Wilson, Princess Margaret Cancer Centre, Toronto, ON, Canada; and Department of Medical Biophysics, University of therapy (PTT) in the intact state, fluorescence imaging after Toronto, Toronto, ON, Canada unquenching, and positron emission tomography via 64Cu Open Access. © 2021 Keegan Guidolin et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution 4.0 International License. 3162 K. Guidolin et al.: Porphysomes are effective photosensitizers for PDT chelation or magnetic resonance imaging via Mn chelation 2 Materials and methods in either state [1–8]. Disrupted PSs have also been shown to produce reactive oxygen species upon irradiation at the 2.1 Cell culture and animal models main 671 nm pyro absorption wavelength, providing a rationale for their use as photodynamic sensitizers [8]. PSs A549 human lung cancer cells (American Type Culture Collection, conjugated to folate have been used to deliver target- Manassas, VA, USA) were cultured in F12K media supplemented with triggered PDT in different tumor models. Limited prior fetal bovine serum at a final concentration of 10% and prepared for studies of the untargeted PSs showed low PDT efficacy; injection in a 1 : 1 mixture of phosphate buffered saline and Matrigel Foxn1nu however, these studies were neither systematic nor opti- (Corning Inc., Corning, NY, USA). Female Hsd:athymic nude mice (Envigo, Indianapolis, IN, USA), aged 8–10 weeks, underwent mized [2, 8]. subcutaneous injection of 5 × 106 A549 cells in the right shoulder. Since the activation wavelength for both PS-PTT and Tumor growth was monitored and, upon reaching 5 mm in the PS-PDT is 671 nm, PS holds the potential to be the first single maximum dimension, the mice were randomized into various treatment agent, single wavelength, all-organic photosensitizer that and control groups. An a priori humane endpoint of 500 mm3 maximum can accomplish both PTT and PDT using a dual population tumor volume was set. All animal studies were conducted in compli- of intact PSs in the extracellular matrix and disrupted PSs in ance with institutional guidelines (University Health Network, Toronto, Canada). the intracellular space (Figure 1). It must first be established that untargeted PSs can accomplish PDT. Hence, the objectives of the present study were to test if PSs can be used 2.2 Photosensitizer and light administration as a practical PDT agent, to determine the optimum treat- ment conditions for this, and to compare PS-PDT to PDT Porphysomes were prepared according to previously published pro- tocols [1]. Photofrin (PHO) was generously provided by Pinnacle Bi- using Photofrin (PHO, Pinnacle Biologics, Bannockburn, IL, ologics (Bannockburn, IL). Following randomization, photosensitizer USA), a long-established, clinically-approved, porphyrin- was administered intravenously by bolus tail vein injection at an based PDT agent [9–12]. appropriate drug-light interval (DLI) before light irradiation. PS was Figure 1: Schematic diagram of the distribution and activity of porphysome nanoparticles and dissociated pyro-lipid in the tumor. When irradiated with 671 nm light, the intact porphysomes in the extracellular space respond photothermally (lower panel, right), while the 1 disrupted intracellular porphysomes (pyro-lipid) are unquenched and generate reactive oxygen species (ROS; e.g., O2) for photodynamic therapy (lower panel, left). K. Guidolin et al.: Porphysomes are effective photosensitizers for PDT 3163 administered in doses of 5, 7.5 or 10 mg (of pyropheophorbide-lipid) 2.5 Analysis per kg of animal body weight, and PHO was administered at 5 mg/kg of animal body weight; each was diluted with phosphate-buffered saline In each animal, the tumor volume post treatment was normalized to to a total volume of 250 μl per dose. Diode lasers at 671 and 630 nm the pre-treatment volume. Complete response was defined as the were used for PS and PHO activation, respectively (Figures S1 and S2). absence of detectable tumor upon dissection at the end of the 30-day Under general anesthesia (2–3% inhaled isoflurane) and with minimal follow-up period (i.e., cure). ANOVA was performed to assess for ambient lighting, the treatment light was delivered via an optical fiber differences in the relative tumor size between groups. Fisher’s exact as a 10 mm diameter circular spot centered on the tumor. The energy test was used to compare cure rates between groups. Log-rank density was measured prior to each treatment using a calibrated power (Mantel-Cox) tests were conducted for comparison of survival curves. meter (Thorlabs Inc., Newton, NJ, USA). A total light dose of 135 J/cm2 Statistical analysis was performed using GraphPad Prism software; was delivered at 100 mW/cm2 over 1350 s (22.5 min). Animals were p < 0.05 were considered significant. protected from scattered light using an opaque mask with a 10 mm diameter hole. 2.3 Study metrics 3 Results Following treatment, the mice were followed for 30 days and the tumor In the first experiment, the maximum increase in tumor size was measured using calipers 3 times per week. Photographs of surface temperature recorded at any time during treatment tumors were taken, and the animals were weighed on each occasion. (n = 3) was 1.8 °C. All PS-PDT treated tumors demonstrated fi Humane sacri ce by anesthetic overdose was carried out after 30 days at least partial response, with growth curves that were or if tumors reached 500 mm3 volume, whichever came first. Animals significantly different from the untreated groups: Figure 2. were considered to have reached a “mortality” endpoint if they required sacrifice because their tumor volume was >500 mm3. The strongest response was observed in the group treated with 10 mg/kg PS at 24 h DLI; followed by 7.5 mg/kg at 24 h, 5 mg/kg at 24 h and 10 mg/kg at 48 h. Tumor size was 2.4 Study aims the smallest at 10–15 d post treatment, at which time the distinction between the treatment groups was most 2 The first aim of this study was to confirm that irradiation at 100 mW/cm evident; tumor regrowth was evident thereafter. Figure 3 and the highest planned PS dose (10 mg/kg) and 24 h drug-light interval compares the tumor responses at each PS dose with follow- (DLI) did not produce significant thermal conversion.
Load more

Recommended publications
  • Prospects of Nanomaterials in Medicinal Photo-Physical-Chemistry
    Organic and Medicinal Chemistry International Journal ISSN 2474-7610 Mini Review Organic & Medicinal Chem IJ Volume 10 Issue 5 - June 2021 Copyright © All rights are reserved by Abdelaziz Boulesbaa DOI: 10.19080/OMCIJ.2020.10.555796 Prospects of Nanomaterials in Medicinal Photo-Physical-Chemistry Pouya Bahrami, Dillon Contreras and Abdelaziz Boulesbaa* Department of Chemistry & Biochemistry, California State University Northridge, USA Submission: April 12, 2021; Published: June 08, 2021 *Corresponding author: Abdelaziz Boulesbaa, Department of Chemistry & Biochemistry, California State University Northridge, 18111 Nordhoff Street, Northridge, CA 91330-8262, USA Abstract noble metal nanoparticles (MNPs) and semiconductor quantum dots (QDs) have been used in medical diagnostics, photothermal therapy (PTT), drugDuring delivery, the and last biolabeling. few decades, In nanotechnologya PTT process, the and tumor lasers area have is made exposed their to way an infrared into many laser applications, with a frequency including selected the medical accordingly field. Forwith instance, respect to the localized surface plasmon resonance (LSPR) of the MNPs injected around the tumor; the local increase in the temperature of MNPs may lead to the burning of the surrounding tumor cells. Although several research efforts have targeted these concepts, most of the investigations and their biofunctionalization. Here, we present an opinion to shed light on the fundamental photophysical and photochemical aspects of the were limited to the examination of
    [Show full text]
  • Metal Organic Framework-Coated Gold Nanorod As an On-Demand Drug
    Huang et al. J Nanobiotechnol (2021) 19:219 https://doi.org/10.1186/s12951-021-00961-x Journal of Nanobiotechnology RESEARCH Open Access Metal organic framework-coated gold nanorod as an on-demand drug delivery platform for chemo-photothermal cancer therapy Junfeng Huang1,2†, Zhourui Xu3†, Yihang jiang3, Wing‑cheung Law4, Biqin Dong5, Xierong Zeng1, Mingze Ma3, Gaixia Xu3*, Jizhao Zou1* and Chengbin Yang3* Abstract Chemo‑photothermal therapy based on nanoparticles has emerged as a promising strategy for cancer treatment. However, its therapeutic efcacy and application potential are largely subjected to the uncontrollability and biotoxic‑ ity of functional nanoplatforms. Herein, a novel biocompatible and biodegradable metal organic framework (MOF), which was constructed by growing crystalline zeolitic imidazolate framework‑8 on gold nanoroad (Au@ZIF‑8), was designed and fabricated for efcient drug loading and controlled release. Owing to the large surface area and guest‑ matching pore size of ZIF‑8, doxorubicin (DOX) was successfully loaded into the Au@ZIF‑8 with a high drug load‑ ing efciency of ~ 37%. Under NIR light or weakly acidic environment, the ZIF‑8 layer was quickly degraded, which resulted in an on‑demand drug release in tumour site. More importantly, under the irradiation of near infrared (NIR) laser, highly efcient cancer treatment was achieved in both in vitro cell experiment and in vivo tumour‑bearing nude mice experiment due to the synergistic efect of photothermal (PTT) therapy and chemotherapy. In addition, the in vivo study revealed the good biocompatibility of Au@ZIF‑8. This work robustly suggested that Au@ZIF‑8 could be further explored as a drug delivery system for chemo‑photothermal synergistic therapy.
    [Show full text]
  • Iron Hydroxide/Oxide-Reduced Graphene Oxide Nanocomposite for Dual-Modality Photodynamic and Photothermal Therapy in Vitro and in Vivo
    nanomaterials Article Iron Hydroxide/Oxide-Reduced Graphene Oxide Nanocomposite for Dual-Modality Photodynamic and Photothermal Therapy In Vitro and In Vivo Wei-Jane Chiu 1, Yi-Chun Chen 2, Chih-Ching Huang 1,3,4 , Lingyan Yang 5 , Jiantao Yu 5, Shih-Wei Huang 6,7,8 and Chia-Hua Lin 2,* 1 Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan; [email protected] (W.-J.C.); [email protected] (C.-C.H.) 2 Department of Biotechnology, National Formosa University, Yunlin 63208, Taiwan; [email protected] 3 Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 20224, Taiwan 4 School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan 5 Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; [email protected] (L.Y.); [email protected] (J.Y.) 6 Department of Electronics, Cheng Shiu University, Kaohsiung 83347, Taiwan; [email protected] 7 Center for Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu University, Kaohsiung 83347, Taiwan 8 Super Micro Research and Technology Center, Cheng Shiu University, Kaohsiung 83347, Taiwan * Correspondence: [email protected]; Tel.: +886-5-6315-558 Citation: Chiu, W.-J.; Chen, Y.-C.; Abstract: Minimal invasive phototherapy utilising near-infrared (NIR) laser to generate local reactive Huang, C.-C.; Yang, L.; Yu, J.; Huang, oxygen species (ROS) and heat has few associated side effects and is a precise treatment in cancer S.-W.; Lin, C.-H.
    [Show full text]
  • Photosensitizer-Functionalized Nanocomposites for Light-Activated Cancer Theranostics
    International Journal of Molecular Sciences Review Photosensitizer-Functionalized Nanocomposites for Light-Activated Cancer Theranostics Banendu Sunder Dash 1 , Suprava Das 1 and Jyh-Ping Chen 1,2,3,4,* 1 Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 33302, Taiwan; [email protected] (B.S.D.); [email protected] (S.D.) 2 Craniofacial Research Center, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou, Kwei-San, Taoyuan 33305, Taiwan 3 Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33305, Taiwan 4 Department of Materials Engineering, Ming Chi University of Technology, Tai-Shan, New Taipei City 24301, Taiwan * Correspondence: [email protected]; Tel.: +886-3-2118800 Abstract: Photosensitizers (PSs) have received significant attention recently in cancer treatment due to its theranostic capability for imaging and phototherapy. These PSs are highly responsive to light source of a suitable wavelength for image-guided cancer therapy from generated singlet oxygen and/or thermal heat. Various organic dye PSs show tremendous attenuation of tumor cells during cancer treatment. Among them, porphyrin and chlorophyll-based ultraviolet-visible (UV-Vis) dyes are employed for photodynamic therapy (PDT) by reactive oxygen species (ROS) and free radicals generated with 400–700 nm laser lights, which have poor tissue penetration depth. To enhance the efficacy of PDT, other light sources such as red light laser and X-ray have been suggested; nonetheless, it is still a challenging task to improve the light penetration depth for deep tumor treatment.
    [Show full text]
  • A Bibliometric Analysis and Visualization of Photothermal Therapy on Cancer
    1215 Original Article A bibliometric analysis and visualization of photothermal therapy on cancer Xiaoyan Wang1#, Dan Li2#, Xinhe Huang3, Qi Luo4, Xue Li5, Xianqin Zhang6, Lin Zhang7,8 1Department of Pathology, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu, China; 2School of Laboratory Medicine, Chengdu Medical College, Chengdu, China; 3School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China; 4School of Biomedical Sciences, Chengdu Medical College, Chengdu, China; 5School of Pharmacy, Chengdu Medical College, Chengdu, China; 6School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China; 7College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China; 8Department of Pharmacy, Shaoxing People’s Hospital, Zhejiang University School of Medicine, Shaoxing, China Contributions: (I) Conception and design: L Zhang; (II) Administrative support: None; (III) Provision of study materials or patients: None; (IV) Collection and assembly of data: X Wang, D Li; (V) Data analysis and interpretation: Q Luo, X Li, X Zhang; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. #These authors contributed equally to this work. Correspondence to: Lin Zhang, PhD. Professor, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China. Email: [email protected]. Background: Cancer is one of the most lethal diseases in the world, and photothermal therapy was reported recently as a new and effective therapy for cancer. This study offers the bibliometric and visualization analysis of photothermal therapy on cancer. Methods: A record of 6,233 papers in this field from 1995 to 2019 was obtained based on the Web of Science Core Collection (WoSCC).
    [Show full text]
  • Ablation of Hypoxic Tumors with Dose-Equivalent Photothermal, but Not Photodynamic, Therapy Using a Nanostructured Porphyrin Assembly ^ ) Cheng S
    ARTICLE Ablation of Hypoxic Tumors with Dose-Equivalent Photothermal, but Not Photodynamic, Therapy Using a Nanostructured Porphyrin Assembly ^ ) Cheng S. Jin,†,‡,§ Jonathan F. Lovell,§, Juan Chen,† and Gang Zheng†,‡,§, ,* †Ontario Cancer Institute, Campbell Family Cancer Research Institute and Techna Institute, University Health Network (UHN), Toronto, Canada M5G 2M9, ‡Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Canada M5S 3M2, §Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada M5S 1A1, ^Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260-2050, United States, and Department) of Medical Biophysics, University of Toronto, Toronto, Canada M5G 1L7 ABSTRACT Tumor hypoxia is increasingly being recognized as a characteristic feature of solid tumors and significantly complicates many treatments based on radio-, chemo-, and phototherapies. While photodynamic therapy (PDT) is based on photosensitizer interactions with diffused oxygen, photothermal therapy (PTT) has emerged as a new phototherapy that is predicted to be independent of oxygen levels within tumors. It has been challenging to mean- ingfully compare these two modalities due to differences in contrast agents and irradiation parameters, and no comparative in vivo studies have been performed until now. Here, by making use of recently developed nanostructured self-quenched porphysome nanoparticles, we were able to directly compare PDT and PTT using matched light doses and matched porphyrin photosensitizer doses (with the photosensitizer being effective for either PTT or PDT based on the existence of nanostructure or not). Therefore, we demonstrated the nanostructure-driven conversion from the PDT singlet oxygen generating mechanism of porphyrin to a completely thermal mechanism, ideal for PTT enhancement.
    [Show full text]
  • Near-Infrared Photothermal
    www.nature.com/scientificreports OPEN Near-infrared photothermal/ photodynamic therapy with indocyanine green induces Received: 17 May 2017 Accepted: 5 October 2017 apoptosis of hepatocellular Published: xx xx xxxx carcinoma cells through oxidative stress Chikara Shirata, Junichi Kaneko, Yoshinori Inagaki, Takashi Kokudo, Masumitsu Sato, Sho Kiritani, Nobuhisa Akamatsu, Junichi Arita, Yoshihiro Sakamoto, Kiyoshi Hasegawa & Norihiro Kokudo Indocyanine green (ICG) is a photothermal agent, photosensitizer, and fuorescence imaging probe which shows specifc accumulation in hepatocellular carcinoma (HCC) cells. We recently developed a photodynamic therapy (PDT) using ICG and near-infrared (NIR) laser as a new anti-cancer treatment for HCC. However, the molecular mechanism underlying this efect needs to be elucidated. HuH-7 cells, a well-diferentiated human HCC cell line, were transplanted subcutaneously into BALB/c-nu/nu mice for in vivo experiment. ICG was administered 24 h before NIR irradiation. The irradiation was performed at three tumor locations by 823-nm NIR laser on days 1 and 7. The temperature of HuH-7 xenografts increased to 48.5 °C 3 minutes after ICG-NIR irradiation start. Reactive oxygen species (ROS) production was detected after ICG-NIR irradiation both in vitro and in vivo. There was certain anti-tumor efect and ROS production even under cooling conditions. Repeated NIR irradiation increased the cell toxicity of ICG-NIR therapy; the mean tumor volume on day 9 was signifcantly smaller after ICG-NIR irradiation compared to tumor without irradiation (87 mm3 vs. 1332 mm3; p = 0.01) in HCC mice xenografts model. ICG-NIR therapy induced apoptosis in HCC cells via a photothermal efect and oxidative stress.
    [Show full text]
  • Upconversion NIR-II Fluorophores for Mitochondria-Targeted Cancer
    ARTICLE https://doi.org/10.1038/s41467-020-19945-w OPEN Upconversion NIR-II fluorophores for mitochondria-targeted cancer imaging and photothermal therapy Hui Zhou 1,2,7, Xiaodong Zeng 1,3,7, Anguo Li1, Wenyi Zhou1,3, Lin Tang1, Wenbo Hu 4, Quli Fan 4, ✉ ✉ Xianli Meng5, Hai Deng 6, Lian Duan1, Yanqin Li1, Zixin Deng1, Xuechuan Hong 1,2 & Yuling Xiao 1,3 fl 1234567890():,; NIR-II uorophores have shown great promise for biomedical applications with superior in vivo optical properties. To date, few small-molecule NIR-II fluorophores have been dis- covered with donor-acceptor-donor (D-A-D) or symmetrical structures, and upconversion- mitochondria-targeted NIR-II dyes have not been reported. Herein, we report development of D-A type thiopyrylium-based NIR-II fluorophores with frequency upconversion luminescence (FUCL) at ~580 nm upon excitation at ~850 nm. H4-PEG-PT can not only quickly and effectively image mitochondria in live or fixed osteosarcoma cells with subcellular resolution at 1 nM, but also efficiently convert optical energy into heat, achieving mitochondria-targeted photothermal cancer therapy without ROS effects. H4-PEG-PT has been further evaluated in vivo and exhibited strong tumor uptake, specific NIR-II signals with high spatial and temporal resolution, and remarkable NIR-II image-guided photothermal therapy. This report presents the first D-A type thiopyrylium NIR-II theranostics for synchronous upconversion- mitochondria-targeted cell imaging, in vivo NIR-II osteosarcoma imaging and excellent photothermal efficiency. 1 State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), Hubei Provincial Key Laboratory of Developmentally Originated Disease, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.
    [Show full text]
  • Magnetic Resonance Imaging Guidance for Laser Photothermal Therapy
    Journal of Biomedical Optics 13͑4͒, 044033 ͑July/August 2008͒ Magnetic resonance imaging guidance for laser photothermal therapy Yichao Chen Abstract. Temperature distribution is a crucial factor in determining University of Central Oklahoma the outcome of laser phototherapy in cancer treatment. Magnetic College of Mathematics and Science resonance imaging ͑MRI͒ is an ideal method for 3-D noninvasive tem- Department of Engineering and Physics 100 North University Drive perature measurement. A 7.1-T MRI was used to determine laser- Edmond, OK 73034 induced high thermal gradient temperature distribution of target tissue with high spatial resolution. Using a proton density phase shift method, thermal mapping is validated for in vivo thermal measure- Surya C. Gnyawali ment with light-absorbing enhancement dye. Tissue-simulating phan- Oklahoma State University tom gels, biological tissues, and tumor-bearing animals were used in Department of Physics the experiments. An laser was used to irradiate the samples, Stillwater, Oklahoma 73019 805-nm with laser power in the range of 1to3W. A clear temperature distri- bution matrix within the target and surrounding tissue was obtained Feng Wu with a specially developed processing algorithm. The temperature Chongqing Medical University mapping showed that the selective laser photothermal effect could Institute of Ultrasonic Engineering in Medicine result in temperature elevation in a range of 10 to 45°C. The tem- and perature resolution of the measurement was about 0.37°C with Clinical Center for Tumor Therapy of 2nd Affiliated Hospital 0.4-mm spatial resolution. The results of this study provide in vivo Medical College Road thermal information and future reference for optimizing laser dosage Chongquing 400016, China and dye concentration in cancer treatment.
    [Show full text]
  • PHOTOTHERAPY TECHNIQUES for CANCER DIAGNOSIS and TREATMENT Saba Goodarzi1 and Masoud Mozafari2
    PHOTOTHERAPY TECHNIQUES FOR CANCER DIAGNOSIS AND TREATMENT Saba Goodarzi1 and Masoud Mozafari2 1. Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology 2. Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC) 1. INTRODUCTION Light plays a significant role in our life. It is used in compact disc (CD) players, in which a laser reflecting off a CD converts the returning signal into music. In grocery store checkout lines, laser beams read bar codes for prices. Laser printers record images on paper by using laser beams. Light is the foundation of the technology which allows computers and telephones to be connected to one another over fiber-optic cables. And light is used in medicine, to produce images used in hospitals and in lasers that perform eye surgery and also cancer treatment. Light therapy or phototherapy contains exposure to daylight or to specific wavelengths of light using polychromatic polarized light, lasers, light-emitting diodes, fluorescent lamps and etc. Phototherapy is a technique of medical treatment in which light is used to treat diseases such as cancers and peripheral infections to normalize the body and relieve the depression. 2. CANCER TREATMENT Cancer is defined as an uncontrolled growth of cells. The uncontrolled growth of body cells generates lumps or masses of tissue called tumors. There more than 200 types of cancer including skin cancer, lung cancer, prostate cancer, breast cancer, and colon cancer. Cancer is one of the most terrible diseases in the world and every year millions of people are dying because of this disease.
    [Show full text]
  • Magnetic Prussian Blue Nanoparticles As Novel Theranostic Agents for Cancer
    Magnetic Prussian Blue Nanoparticles as Novel Theranostic Agents for Cancer Shraddha Kale B.E. in Biomedical Engineering, June 2013, Mumbai University A Thesis submitted to The Faculty of The School of Engineering and Applied Science of The George Washington University in partial fulfillment of the requirements for the degree of Master of Science May 15, 2016 Thesis directed by Rohan Fernandes Principal Investigator, Children’s National Medical Center Jason Zara Associate Professor of Engineering and Applied Science © Copyright 2016 Shraddha Kale All rights reserved Dedication To my parents: for their love, guidance, support, and patience. iii Abstract of Thesis Magnetic Prussian Blue Nanoparticles as Novel Theranostic Agent for Cancer Theranostic nanoparticles simultaneously possess therapeutic and diagnostic capabilities. These nanoparticles offer the potential for more sensitive diagnoses and improved treatment results in human diseases, using a single platform. In the past, Prussian blue nanoparticles were synthesized and were used independently for imaging and therapy applications in the field of cancer 4-8. Inspired by the potential for improved outcomes using theranostic nanoparticles, we have developed a single theranostic nanoparticle platform that integrates both imaging and therapy capabilities. The objective of this paper is to synthesize composite, core shell magnetic gadolinium Prussian blue nanoparticles that function as excellent MRI contrast agents in both T1 and T2-weighted sequences and as photo-thermal therapy agents. The application of these nanoparticles would be for biomedical imaging, diagnostic and therapeutic on a nanoscale. The nanoparticles can be combined with medical imaging for detecting tumor sites in the body and also provide less invasive and more precise means for curing Neuroblastoma cancers.
    [Show full text]
  • In-Situ Temperature Monitoring with Photoacoustics During Photothermal
    In-situ temperature monitoring with photoacoustics during photothermal therapy and perspectives for glioblastoma treatment monitoring Khaled Metwally, Chiara Bastiancich, Noé Dumas, Florian Correard, Anthony Novell, Florence Chaspoul, Nicola Jones, Gleb Tselikov, Anton Popov, Ahmed Al-Kattan, et al. To cite this version: Khaled Metwally, Chiara Bastiancich, Noé Dumas, Florian Correard, Anthony Novell, et al.. In- situ temperature monitoring with photoacoustics during photothermal therapy and perspectives for glioblastoma treatment monitoring. Opto-Acoustic Methods and Applications in Biophotonics, Jun 2019, Munich, France. pp.8, 10.1117/12.2527060. hal-02415947 HAL Id: hal-02415947 https://hal.archives-ouvertes.fr/hal-02415947 Submitted on 18 Mar 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie In-situ temperature monitoring with photoacoustics during photothermal therapy and perspectives for glioblastoma treatment monitoring K. Metwally, C. Bastiancich, N. Dumas, F. Correard, A. Novell, et al. K. Metwally, C. Bastiancich, N. Dumas, F. Correard, A. Novell, F. Chaspoul, N. Jones, G. Tselikov, A. Popov, A. Al-Kattan, A. V. Kabashin, B. Larrat, D. Braguer, S. Mensah, M.-A. Estève, A. Da Silva, "In-situ temperature monitoring with photoacoustics during photothermal therapy and perspectives for glioblastoma treatment monitoring," Proc.
    [Show full text]