DOCSLIB.ORG

Effects of Smoking and Gender on Tetrahydroisoquinolines and Beta-Carbolines in a Healthy Population and During Alcohol Detoxification

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effects of Smoking and Gender on Tetrahydroisoquinolines and Beta-Carbolines in a Healthy Population and During Alcohol Detoxification Virginia Commonwealth University VCU Scholars Compass Theses and Dissertations Graduate School 2008 Effects of Smoking and Gender on Tetrahydroisoquinolines and Beta-Carbolines in a Healthy Population and During Alcohol Detoxification Satjit Singh Brar Virginia Commonwealth University Follow this and additional works at: https://scholarscompass.vcu.edu/etd Part of the Pharmacy and Pharmaceutical Sciences Commons © The Author Downloaded from https://scholarscompass.vcu.edu/etd/902 This Dissertation is brought to you for free and open access by the Graduate School at VCU Scholars Compass. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of VCU Scholars Compass. For more information, please contact [email protected]. © Satjit Singh Brar 2008 All Rights Reserved EFFECTS OF SMOKING AND GENDER ON TETRAHYDROISOQUINOLINES AND β–CARBOLINES IN A HEALTHY POPULATION AND DURING ALCOHOL DETOXIFICATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University. by SATJIT SINGH BRAR B.S., University of California at Santa Barbara, 1998 Director: Jürgen Venitz, MD, Ph.D. Associate Professor, Department of Pharmaceutics Virginia Commonwealth University Richmond, Virginia May 2008 ii Acknowledgements Jürgen Venitz, first and foremost, for giving me the opportunity to pursue graduate studies under his guidance. His mentoring in academics and research has been truly motivating and inspirational. Members of my graduate committee, Drs. Jürgen Venitz, John Rosecrans, Patricia Slattum, Vijay Ramchandani, H. Thomas Karnes, and Hee-Yong Kim for their efforts, patience and time for serving on my committee. Patricia Slattum for her advice and encouragement. She has provided valuable aid over the years by giving guidance throughout my tenure in the Pharm.D./Ph.D. program. John Rosecrans for his charitable partial funding of my clinical study from his personal research monies. Moreover, his enthusiasm and interests for the projects we conducted was unwavering throughout all the years. Vijay Ramchandani for bringing my dissertation project to fruition. He has been implemental in the initiation and completion of a major part of this project. His scientific guidance, career advice and emotional support during my involvement with NIH have been priceless. Without his help, it would have been a much longer experience. Hee-Yong Kim for hosting me for a year in her lab and having patience during my training. Her support assuaged my frustrations during my experience at NIH. H. Thomas Karnes for his advice on assay methodology and validation. The academic discourse between him and I gave me a larger appreciation for bioanalytical quantification. John Clore for generously serving as the Medical Monitor for my study. In addition, the wonderful staff of the VCU-GCRC: Janet, Denise, Gloria, Yvonne, Glenda, Lou, and Pat, all of which, were of great help and support in the conduct of my clinical study. Performing my clinical study at the GCRC was a pleasurable experience. General Clinical Research Center Grant M01 RR00065, NCRR, NIH and GCRC staff support and supplies. All those at the Laboratory of Clinical and Translational Studies at NIH-NIAAA who were very helpful in gathering patient samples and subject information for this project. In particular, thanks to David Herion for serving as the Principal Investigator and Ted iii George for being the Medical Monitor on this project, Denise Gates-Nee for her timely data management skills and Monte Phillips for organization of the transfer of samples. I would like to thank all other research and technical laboratory staff for all of their help throughout the conduct of this study. All those at the Laboratory of Molecular Signaling at NIH-NIAAA who were very cooperative and accommodating. The scientific training received from Dr. Jeongrim Lee was immeasurable. Her willingness to help troubleshoot and to provide me with advice, scientific and personal, throughout my project was very helpful. She is an amazing person. The pre-doctoral NIH Intramural Research Training Award (IRTA) Program that supplied me with the financial support during my stay at NIH. Jocelyn Leu for samples and data from her clinical study to include as part of this project. She was an important factor in my initial clinical training and has provided appreciable advice throughout my graduate schooling. The Deans Office and Department of Pharmaceutics Office, specifically, Mia, Laura, Keyetta, for their patience and management of all that is administrative throughout my experience here at VCU. All the members of the PK-PD Research Group, present and past, particularly Pankaj, David, Lokesh, Angela, Prajakta, and Taghreed for being very supportive throughout the years and running around for me when I needed the help (thanks Dave!). My dear and closest friends including Dan, Lans, Ali, Gregg, Aviva, Scott, Kim, Kate, Toby, Josh, Stephanie, Art and others who taught me that life is more than just work and helped me relax throughout this process. Their encouragement and support has established them as life-long friends in my book. To all my family members who have been very supportive and eager for me to finish. In particular, my sisters Anjum and Amrita for believing in me and providing me with love and emotional support throughout. My parents Balbir and Raminder Brar who have always unconditionally loved me and encouraged me in my life pursuits. Your love and support are among my most cherished memories. Last, but not least, a very extraordinary person who has been patient, understanding and stood beside me unconditionally, sometimes from a far distance, my soon-to-be wife and best friend, Heidi. iv Table of Contents Page Acknowledgements............................................................................................................. ii List of Tables ................................................................................................................... xiii List of Figures...................................................................................................................xxi List of Abbreviations .......................................................................................................xxx Abstract.........................................................................................................................xxxiv Chapter 1 Introduction and Background ........................................................................................1 1.1 Introduction.............................................................................................................1 1.2 Background.............................................................................................................2 1.2.1 Current Biomarkers for Smoking Dependence and Alcoholism ...................2 1.2.2 The “Reward” pathway..................................................................................5 1.2.3 Tetrahydroisoquinolines (TIQ’s: R/S-Salsolinol)..........................................8 1.2.3a Chemistry and biosynthesis .............................................................8 1.2.3b SAL in-vitro pharmacology...........................................................12 1.2.3c SAL in-vivo animal and human studies .........................................14 1.2.4 β-carbolines (harman and norharman).........................................................20 1.2.4a Chemistry and biosynthesis ...........................................................20 1.2.4b β-carbolines in-vitro pharmacology...............................................23 1.2.4c β-carbolines in-vivo animal and human studies.............................24 1.3 TIQ’s and β-carbolines summary ..........................................................................28 v 2 Research Hypothesis....................................................................................................31 2.1 Hypothesis ............................................................................................................31 2.1.1 Study #1 Hypothesis....................................................................................33 2.1.2 Study #2 Hypothesis....................................................................................34 2.2 Rationale and Significance ...................................................................................36 3 Assay development of the β-carbolines in human plasma...........................................39 3.1 Introduction – Selection of Analytical method.....................................................39 3.2 Physicochemical Characteristics of Harman and Norharman ..............................43 3.3 HPLC-Fluorescence Detection Method Development .........................................45 3.3.1 Fluorescence Detection (FD) Experiments..................................................45 3.3.1a Materials and Reagents..................................................................47 3.3.1b Equipment......................................................................................48 3.3.1c Preparation of Solutions and Standards .........................................48 3.3.1d Methods .........................................................................................50 3.3.1e Results............................................................................................51 3.3.1f Discussion ......................................................................................59
Load more

Recommended publications
  • Aldrich FT-IR Collection Edition I Library
    Aldrich FT-IR Collection Edition I Library Library Listing – 10,505 spectra This library is the original FT-IR spectral collection from Aldrich. It includes a wide variety of pure chemical compounds found in the Aldrich Handbook of Fine Chemicals. The Aldrich Collection of FT-IR Spectra Edition I library contains spectra of 10,505 pure compounds and is a subset of the Aldrich Collection of FT-IR Spectra Edition II library. All spectra were acquired by Sigma-Aldrich Co. and were processed by Thermo Fisher Scientific. Eight smaller Aldrich Material Specific Sub-Libraries are also available. Aldrich FT-IR Collection Edition I Index Compound Name Index Compound Name 3515 ((1R)-(ENDO,ANTI))-(+)-3- 928 (+)-LIMONENE OXIDE, 97%, BROMOCAMPHOR-8- SULFONIC MIXTURE OF CIS AND TRANS ACID, AMMONIUM SALT 209 (+)-LONGIFOLENE, 98+% 1708 ((1R)-ENDO)-(+)-3- 2283 (+)-MURAMIC ACID HYDRATE, BROMOCAMPHOR, 98% 98% 3516 ((1S)-(ENDO,ANTI))-(-)-3- 2966 (+)-N,N'- BROMOCAMPHOR-8- SULFONIC DIALLYLTARTARDIAMIDE, 99+% ACID, AMMONIUM SALT 2976 (+)-N-ACETYLMURAMIC ACID, 644 ((1S)-ENDO)-(-)-BORNEOL, 99% 97% 9587 (+)-11ALPHA-HYDROXY-17ALPHA- 965 (+)-NOE-LACTOL DIMER, 99+% METHYLTESTOSTERONE 5127 (+)-P-BROMOTETRAMISOLE 9590 (+)-11ALPHA- OXALATE, 99% HYDROXYPROGESTERONE, 95% 661 (+)-P-MENTH-1-EN-9-OL, 97%, 9588 (+)-17-METHYLTESTOSTERONE, MIXTURE OF ISOMERS 99% 730 (+)-PERSEITOL 8681 (+)-2'-DEOXYURIDINE, 99+% 7913 (+)-PILOCARPINE 7591 (+)-2,3-O-ISOPROPYLIDENE-2,3- HYDROCHLORIDE, 99% DIHYDROXY- 1,4- 5844 (+)-RUTIN HYDRATE, 95% BIS(DIPHENYLPHOSPHINO)BUT 9571 (+)-STIGMASTANOL
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2009/0318437 A1 Gaeta Et Al
    US 20090318437A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2009/0318437 A1 Gaeta et al. (43) Pub. Date: Dec. 24, 2009 (54) SUBSTITUTED PYRAZOLO15-APYRIDINE (60) Provisional application No. 60/811,604, filed on Jun. COMPOUNDS AND THER METHODS OF 6, 2006. USE (76) Inventors: Federico C.A. Gaeta, Mountain Publication Classification View, CA (US); Matthew Gross, (51) Int. Cl. Vallejo, CA (US); Kirk W. A63L/437 (2006.01) Johnson, Moraga, CA (US) C07D 47L/04 (2006.01) Correspondence Address: C07D 413/14 (2006.01) King & Spalding LLP A 6LX 3/5.377 (2006.01) P.O. BOX 889 A6IP II/06 (2006.01) Belmont, CA 94002-0889 (US) (52) U.S. Cl. ...................... 514/233.2:546/121; 544/127; (21) Appl. No.: 12/482,307 514/3OO (22) Filed: Jun. 10, 2009 Related U.S. Application Data (57) ABSTRACT (62) Division of application No. 1 1/810,885, filed on Jun. 6, The present invention is directed to substituted pyrazolo 1.5- 2007, now Pat. No. 7,585,875. apyridines and related methods for their synthesis and use. Patent Application Publication Dec. 24, 2009 Sheet 1 of 2 US 2009/0318437 A1 log threshold withdrawal (mg x 10) s s t | yme as w (s (f) pouseul emerpum 909 Y Patent Application Publication Dec. 24, 2009 Sheet 2 of 2 US 2009/0318437 A1 s o O O war C SSm S.t c) is . E E C C a S. & S. is 25 &(2 52 > w- we t : : log threshold withdrawal (mg x 10) N CN N.
    [Show full text]
  • Possible Interference by Common Odoriferous Foodstuffs in the Determination of Breath-Alcohol Content Using the Intoxilyzer 4011AS
    University of the Pacific Scholarly Commons University of the Pacific Theses and Dissertations Graduate School 1988 Possible interference by common odoriferous foodstuffs in the determination of breath-alcohol content using the Intoxilyzer 4011AS Gail Eileen Jones University of the Pacific Follow this and additional works at: https://scholarlycommons.pacific.edu/uop_etds Part of the Medicine and Health Sciences Commons Recommended Citation Jones, Gail Eileen. (1988). Possible interference by common odoriferous foodstuffs in the determination of breath-alcohol content using the Intoxilyzer 4011AS. University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/2152 This Thesis is brought to you for free and open access by the Graduate School at Scholarly Commons. It has been accepted for inclusion in University of the Pacific Theses and Dissertations by an authorized administrator of Scholarly Commons. For more information, please contact [email protected]. POSSIBLE INI'ERFERENCE BY CXM-m OOORIFERCUS FOODS'IUFFS IN 'lliE DEI'ERMINATICN OF' BREATH-ALCOHOL CCNl'ENl' USIN:; THE INIDXILyzER 40llAS Gail Eileen Jones, B. s. Cameron University, Lawton, Oklahana, 1982 A 'lhesis sutmi.tted in Partial F\llfillmant of the RequirE!!"!Vants for the Degree of M:tster of Science in Toxicology at the University of the Pacific December 2, 1988 This thesis, written and submitted by Gail Eileen Jones is approved for recommendation to the Committee on Graduate Studies, University of the Pacific Dean of the School or Department Chairman: Co-Chairman Co-Chairman Dated__ ()_~__;~~~-+----'/ 9-=(......:.( __ _ TABLE OF CCNI'ENI'S Page LIST OF TABLES •• ii LIST OF FIGURES. • . i ii INI'RODUcriCN • • • • 1 Historical and Theoretical Backgroun::i • 1 Drinking, Driving and the I.aw • • 1 Ethanol : Chemical and Pharrca.cological Properties 10 Physiological Principles and the Detennination of Bl ood ~1.
    [Show full text]
  • Chiral CE Separation of Dopamine-Derived Neurotoxins
    ANALYTICAL SCIENCES FEBRUARY 2005, VOL. 21 115 2005 © The Japan Society for Analytical Chemistry Chiral CE Separation of Dopamine-Derived Neurotoxins Zhe QUAN,* Yaru SONG,* Gladys PETERS,* Ming SHENWU,* Yinghong SHENG,* Huey-Min HWANG,** and Yi-Ming LIU*† *Department of Chemistry, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA **Department of Biology, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA An enantiomeric separation of dopamine-derived neurotoxins by capillary electrophoresis has been developed. Tetrahydroisoquinoline (TIQ), dopamine (DA), (R/S)-1-benzyl-TIQ (BTIQ), (R/S)-6,7-dihydroxy-1-methyl-TIQ (salsolinol, Sal), and (R/S)-6,7-dihydroxy-1, 2-dimethyl-TIQ (N-methyl-salsolinol, NMSal) were studied as model compounds. The CE running buffer (50 mM phosphate buffer at pH 3.0) contained 1.5 M urea and 12 mM β-CD as a chiral selector. During separation, the (R)-enantiomers formed more stable inclusion complexes with β-CD, and thus had a longer migration time than their optical antipodes. It was noticed that the recovery rates of these TIQ derivatives were very poor (< 15%) during protein precipitation, a procedure widely used for cleaning up biological samples. The recovery was significantly improved by pre-mixing the sample with a surfactant (e.g., sodium hexanesulfonate or Triton X-100) to reduce the co-precipitation. The present method in combination with electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied to study samples obtained from in vitro incubation of two catecholamines, dopamine and epinine, with aldehydes forming neurotoxins including (S)- and (R)-NMSal enantiomers. The later is known to induce Parkinsonism in rats.
    [Show full text]
  • Police Powers, Trespass and Expressive Rights Under the Canadian Constitution
    The Peter A. Allard School of Law Allard Research Commons Faculty Publications Allard Faculty Publications 2007 Police Powers, Trespass and Expressive Rights Under the Canadian Constitution W. Wesley Pue Allard School of Law at the University of British Columbia, [email protected] Follow this and additional works at: https://commons.allard.ubc.ca/fac_pubs Part of the Constitutional Law Commons, and the Indigenous, Indian, and Aboriginal Law Commons Citation Details W Wesley Pue, "Police Powers, Trespass and Expressive Rights Under the Canadian Constitution" (2007) [unpublished]. This Working Paper is brought to you for free and open access by the Allard Faculty Publications at Allard Research Commons. It has been accepted for inclusion in Faculty Publications by an authorized administrator of Allard Research Commons. Pue, Trespass & Rights, paper prepared for the Ipperwash Inquiry, 2005-02-04 1 of 96 Trespass and Expressive Rights By. W. Wesley Pue … the rights guaranteed in the Charter erect around each individual, metaphorically speaking, an invisible fence over which the state will not be allowed to trespass. The role of the courts is to map out, piece by piece, the parameters of the fence. • R. v. Morgentaler, [1988] 1 S.C.R. 30, at p. 164, per Wilson J. Paper prepared for The Ipperwash Inquiry, The Honourable Sidney B. Linden, Commissioner, established by the Government of Ontario on November 12, 2003, under the Public Inquiries Act to inquire and report on events surrounding the death of Dudley George, shot during a protest by First Nations representatives at Ipperwash Provincial Park in 1995. Pue, Trespass & Rights, paper prepared for the Ipperwash Inquiry, 2005-02-04 2 of 96 Table of Contents Introduction The Summit of the Americas, 2001 Legal Issues “Authorized by Law” R.
    [Show full text]
  • Successfully Defending an OUI Case
    CHAPTER 10 Successfully Defending an OUI Case David P. Sorrenti, Esq. Sorrenti & Delano, Brockton § 10.1 Introduction ............................................................................... 10–1 § 10.2 The Offense ................................................................................ 10–2 § 10.3 Operation ................................................................................... 10–2 § 10.3.1 Definition .................................................................... 10–2 § 10.3.2 Defense ....................................................................... 10–3 § 10.4 Public Way .................................................................................. 10–4 § 10.4.1 Definition .................................................................... 10–4 § 10.4.2 Stipulation of Proof .................................................... 10–5 § 10.4.3 Defense ....................................................................... 10–5 § 10.5 Under the Influence ................................................................... 10–6 § 10.5.1 Diminished Capacity .................................................. 10–6 (a) General Principles ............................................. 10–6 (b) Opinion Evidence .............................................. 10–7 (c) Erratic Operation ............................................... 10–7 (d) Odor of Alcohol ................................................. 10–7 (e) Red or Glassy Eyes ............................................ 10–8 (f) Slurred Speech ..................................................
    [Show full text]
  • The Neurochemical Effects of Several Carboxylated Tetrahydroisoquinolines
    Loyola University Chicago Loyola eCommons Dissertations Theses and Dissertations 1983 The Neurochemical Effects of Several Carboxylated Tetrahydroisoquinolines Jerome James Hannigan Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_diss Part of the Medicine and Health Sciences Commons Recommended Citation Hannigan, Jerome James, "The Neurochemical Effects of Several Carboxylated Tetrahydroisoquinolines" (1983). Dissertations. 2225. https://ecommons.luc.edu/luc_diss/2225 This Dissertation is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Dissertations by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1983 Jerome James Hannigan The Neurochemical Effects of Several Carboxylated Tetrahydroisoquinolines by Jerome James Hannigan A Dissertation Submitted to the Faculty of the Graduate School of Loyola University of Chicago. in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY June 1983 .,.. ·-. ~ 1983, Jerome James Hannigan ACKNOWLEDGEMENTS I would like to acknowledge Dr. Michael Collins for his role in training me to be a research scientist. His supervision has been helpful in pursuing the answers to the questions addressed by this dissertation. I wish to thank Dr. Byron Anderson for encouraging me to pursue a career in Biochemistry. I have come to share his love for science. I could not have survived graduate school without the help and guidance of the faculty of the Department of Biochemistry. To my fellow students I owe a debt of gratitude.
    [Show full text]
  • Synthesis, Characterization and in Vitro Antimicrobial
    Journal of Saudi Chemical Society (2011) xxx, xxx–xxx King Saud University Journal of Saudi Chemical Society www.ksu.edu.sa www.sciencedirect.com ORIGINAL ARTICLE Synthesis, characterization and in vitro antimicrobial activity of some 1-(substitutedbenzylidene) -4-(4-(2-(methyl/phenyl)-4-oxoquinazolin-3(4H)- yl)phenyl)semicarbazide derivatives Govindaraj Saravanan a,*, Veerachamy Alagarsamy b, Chinnasamy Rajaram Prakash c a Medicinal Chemistry Research Laboratory, Bapatla College of Pharmacy, Jawaharlal Nehru Technological University, Hyderabad, Andhra Pradesh, India b Medicinal Chemistry Research Laboratory, M.N.R. College of Pharmacy, Sangareddy, Andhra Pradesh, India c Department of Medicinal Chemistry, DCRM Pharmacy College, Inkollu, Andhra Pradesh, India Received 19 October 2011; accepted 6 December 2011 KEYWORDS Abstract A series of 1-(substitutedbenzylidene)-4-(4-(2-(methyl/phenyl)-4-oxoquinazolin-3(4H)-yl) Quinazolin-4(3H)-one; phenyl)semicarbazide derivatives were synthesized with the aim of developing potential antimicro- Semicarbazide; bials. It was characterized by FT-IR, 1H NMR, Mass spectroscopy and elemental analysis. In addi- Schiff base; tion, the in vitro antibacterial and antifungal properties were tested against some human pathogenic Antibacterial activity; microorganisms by employing the disc diffusion technique and agar streak dilution method. All title Antifungal activity compounds showed activity against the entire strain of microorganisms. The relationship between the functional group variation and the biological activity of the evaluated compounds were well dis- cussed. Based on the results obtained, compound 5j was found to be very active compared to the rest of the compounds which were subjected to antimicrobial assay. ª 2011 King Saud University. Production and hosting by Elsevier B.V.
    [Show full text]
  • The Impact of Systematically Varying the Duration of Breath Samples During Infrared-Based Alcohol Breath Testing
    Western Michigan University ScholarWorks at WMU Dissertations Graduate College 6-2002 The Impact of Systematically Varying the Duration of Breath Samples during Infrared-Based Alcohol Breath Testing Chris C. Clatterbuck Western Michigan University Follow this and additional works at: https://scholarworks.wmich.edu/dissertations Part of the Equipment and Supplies Commons, Investigative Techniques Commons, and the Other Psychiatry and Psychology Commons Recommended Citation Clatterbuck, Chris C., "The Impact of Systematically Varying the Duration of Breath Samples during Infrared-Based Alcohol Breath Testing" (2002). Dissertations. 1188. https://scholarworks.wmich.edu/dissertations/1188 This Dissertation-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Dissertations by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. THE IMPACT OF SYSTEMATICALLY VARYING THE DURATION OF BREATH SAMPLES DURING INFRARED-BASED ALCOHOL BREATH TESTING by Chris C. Clatterbuck A Dissertation Submitted to the Faculty of The Graduate College m partial fulfillment of the requirements for the Degree of Doctor of Philosophy Department of Counselor Education and Counseling Psychology Western Michigan University Kalamazoo. Michigan June 2002 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. THE IMPACT OF SYSTEMATICALLY VARYING THE DURATION OF BREATH SAMPLES DURING
    [Show full text]
  • Herbert H. Leckie, Jd
    HERBERT H. LECKIE, JD 349 Mountain Road, Lebanon, NJ 08833 (908) 534-8300 [email protected] dwi-dreconsulting.com Skills Summary Over 33 years of experience in “Driving While Under the Influence” investigations. Expertise includes evaluation of both alcohol and drug-related DWI matters. Extensive experience in a number of breath test instruments including the Alcotest 7110 MK 111-C instrument. A former instructor in the Standardized Field Sobriety Tests (SFST’s) wherein there is the ability to evaluate the proper administration and scoring of the tests administered in DWI investigations. Evaluation of “Refusal” cases including procedural issues related to the Subjective Refusal charge. As a former Drug Recognition Expert Instructor and Course Manager, ability to evaluate cases in which an individual has been charged with operating a motor vehicle while under the influence of drugs (both illicit and prescription). Accepted as an expert witness in the United States Federal District Court, numerous Superior Courts as well as numerous municipal courts throughout the State of New Jersey. Also accepted as an expert witness in Supreme Court, New York County as well as New York County Criminal Court. Education Juris Doctorate, Seton Hall University School of Law (2000) • Student Bar Association President (1999-2000) Bachelor of Science, Rutgers University (1995) • Administration of Justice – with Honors Associate of Science, Middlesex County College (1982) • Criminal Justice Career Experience/Training DWI-DRE Consulting Services, Inc. (2018-Present) President Providing instruction, expert review, analysis and testimony in the following areas: Breathalyzer 900/900A, Alcotest 7110 MK III-C, Standardized Field Sobriety Test Procedures and Drug Recognition Expert Evaluation Case Review.
    [Show full text]
  • Step-By-Step Guide to Better Laboratory Management Practices
    Step-by-Step Guide to Better Laboratory Management Practices Prepared by The Washington State Department of Ecology Hazardous Waste and Toxics Reduction Program Publication No. 97- 431 Revised January 2003 Printed on recycled paper For additional copies of this document, contact: Department of Ecology Publications Distribution Center PO Box 47600 Olympia, WA 98504-7600 (360) 407-7472 or 1 (800) 633-7585 or contact your regional office: Department of Ecology’s Regional Offices (425) 649-7000 (509) 575-2490 (509) 329-3400 (360) 407-6300 The Department of Ecology is an equal opportunity agency and does not discriminate on the basis of race, creed, color, disability, age, religion, national origin, sex, marital status, disabled veteran’s status, Vietnam Era veteran’s status or sexual orientation. If you have special accommodation needs, or require this document in an alternate format, contact the Hazardous Waste and Toxics Reduction Program at (360)407-6700 (voice) or 711 or (800) 833-6388 (TTY). Table of Contents Introduction ....................................................................................................................................iii Section 1 Laboratory Hazardous Waste Management ...........................................................1 Designating Dangerous Waste................................................................................................1 Counting Wastes .......................................................................................................................8 Treatment by Generator...........................................................................................................12
    [Show full text]
  • Coercion in Field Sobriety and Breath Testing Cases in Light of Sponar and Shaw
    COERCION IN FIELD SOBRIETY AND BREATH TESTING CASES IN LIGHT OF SPONAR AND SHAW By Ronnie M. Cole, Esquire and Joshua T. Hawkins, Esquire Picture this…your client is on the roadside being asked to take field sobriety tests, or is in a breath test room where he/she is being asked to submit to a DataMaster test. The client wants to refuse but is intimidated, coerced, tricked or misled into complying. What is the remedy? Can the evidence be suppressed? In Sponar v. South Carolina Department of Public Safety , 361 S.C. 35, 603 S.E.2d 412, Ct. App. (2004), the South Carolina Court of Appeals was confronted with that exact issue. Sponar was arrested and charged with DUI and refused a DataMaster test. During the observation period Sponar asked whether he would still go to jail if he took the test, and the officer replied that it did not matter whether he took the test, because he would be going to jail either way. In Town of Mount Pleasant v. Shaw , 315 S.C. 111, 432 S.E.2d 450 (1993), the Supreme Court adopted the following rule: [I]f the arrested person is reasonably informed of his rights, duties and obligations under our implied consent law and he is neither tricked nor misled into thinking he has no right to refuse the test to determine the alcohol content in his blood, urine or breath, the test will generally be held admissible. The court found the officer’s statement to Sponar that he would be going to jail regardless of his decision on whether to submit to the breath test did not inadequately advise Sponar pursuant to South Carolina’s implied consent statute.
    [Show full text]