Polyclonal Antibody to GJC1 / Cx45 (N-Term) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP11580PU-N Polyclonal Antibody to GJC1 / Cx45 (N-term) - Purified

Alternate names: Connexin-45, GJA7, Gap junction alpha-7 protein, Gap junction gamma-1 protein Quantity: 0.4 ml Concentration: lot specific Background: Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua. Uniprot ID: P36383 NCBI: 9606 GeneID: 10052 Host / Isotype: Rabbit / Ig Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GJA7. Remarks: The gene sequence of the antigen is 100 % homologous to mouse. Format: State: Liquid Ig fraction Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS Buffer System: PBS with 0.09% (W/V) sodium azide Applications: ELISA 1:1,000. Western blotting 1:100 - 1:500. Immunohistochemistry 1:50 - 1:100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects GJA7 at N-term. Species: Human, Mouse. Other species not tested.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 2 AP11580PU-N: Polyclonal Antibody to GJC1 / Cx45 (N-term) - Purified

Add. Information: Molecular weigt: 45483 Da Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-GJA7 N-term Pab (AP11580PU-N) in A547 cell lysate. GJA7 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 2