G C A T T A C G G C A T genes Article Multi-Species Phylogeography of Arid-Zone Sminthopsinae (Marsupialia: Dasyuridae) Reveals Evidence of Refugia and Population Expansion in Response to Quaternary Change Linette S. Umbrello 1,2,* , Raphael K. Didham 1,3 , Ric A. How 2,4 and Joel A. Huey 1,2,5 1 School of Biological Sciences, University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia;
[email protected] (R.K.D.);
[email protected] (J.A.H.) 2 Western Australian Museum, Locked Bag 49, Welshpool DC, WA 6986, Australia;
[email protected] 3 CSIRO Health & Biosecurity, Centre for Environment and Life Sciences, Floreat, WA 6014, Australia 4 School of Human Science, University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia 5 Biologic Environmental Survey, 24 Wickham Street, East Perth, WA 6004, Australia * Correspondence:
[email protected] Received: 29 July 2020; Accepted: 19 August 2020; Published: 20 August 2020 Abstract: Historical population contraction and expansion events associated with Pleistocene climate change are important drivers of intraspecific population structure in Australian arid-zone species. We compared phylogeographic patterns among arid-adapted Dasyuridae (Sminthopsis and Planigale) with close phylogenetic relationships and similar ecological roles to investigate the drivers of phylogeographic structuring and the importance of historical refugia. We generated haplotype networks for two mitochondrial (control region and cytochrome b) and one nuclear (omega-globin) gene from samples distributed across each species range. We used FST to test for a genetic population structure associated with the four Pilbara subregions, and we used expansion statistics and Bayesian coalescent skyline analysis to test for signals of historical population expansion and the timing of such events.