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FLRT Proteins Act As Guidance Cues for Migrating Cortical Interneurons

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FLRT Proteins Act As Guidance Cues for Migrating Cortical Interneurons Nom/Logotip de la Universitat on s’ha llegit la tesi FLRT proteins act as guidance cues for migrating cortical interneurons Catherine Fleitas Pérez http://hdl.handle.net/10803/378646 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. Universitat de Lleida Ph.D. Dissertation FLRT PROTEINS ACT AS GUIDANCE CUES FOR MIGRATING CORTICAL INTERNEURONS Catherine Fleitas Pérez Supervisors: Dr. Joaquin Egea Navarro Dr. Carme Espinet Mestre Lleida, 2015 FLRT PROTEINS ACT AS GUIDANCE CUES FOR MIGRATING CORTICAL INTERNEURONS Mèmoria de tesi doctoral presentada per Catherine Fleitas Pérez per optar al grau de Doctor per la Universitat de Lleida (Programa de Doctorat en Salut). Treball realizat a l'Institut de Recerca Biomèdica de Lleida (IRBLleida) a la Unitat Molecular and Developmental Neurobiology del grup consolidad Cicle Cel·lular del Departament de Ciènces Mèdiques Bàsiques de la Universitat de Lleida, sota la direcció del Doctor Joaquim Egea Navarro. El treball realizat en aquesta tesi doctoral ha estat finançat pels projects de reserca del Ministerio de Economía y Competitividad Function of the FLRT Family of LRR Proteins in the Nervous System Development and Connectivity (BFU2010- 1805) i pel Career Integration Grant de la UE (programa Marie Curie) Novel Molecular Mechanisms of Neuron Migration in the Developing Cortex and their Contribution to Related Diseases (Neuromigration, 293980). La doctoranda Catherine Fleitas Pérez ha gaudit durant aquest temps d'una beca predoctoral Jade Plus. Lleida, 23 de Maig de 2015, Doctorand Directores de tesi Catherine Fleitas Pérez Joaquim Egea Navarro Carme Espinet Mestre A mis padres, A abuela Mirtha "¿Para qué, sino para poner paz entre los hombres, han de ser adelantos de la ciencia?". José Martí Acknowledgments A Carme y a Joaquim, que con orgullo digo, MIS TUTORES, les estoy muy agradecida desde antes de venir. No sólo por haberme aceptado como su estudiante, sino porque sin conocerme, confiaron en mí, y me facilitaron llegar hasta aquí. Desde entonces, no han parado de ayudarme y brindarme todo lo que se necesita para sentirse realizado y afortunado haciendo una Tesis lejos de casa, me siento muy feliz por eso. Carme desde el primer día me recibió con mucho cariño. Me enseñó con paciencia y sin ningún tipo de jerarquía las primeras cosas del laboratorio. Su alegría de siempre, sentido del humor, tolerancia, comprensión, apoyo, disposición a brindarme su conocimiento, experiencia y tiempo. Todo eso hizo que me sintiera en confianza, y más que tutora, a la cual respeto mucho, ha sido, mi compañera, con la que compartía el trabajo. Amiga, y a veces la mamá que me ha aconsejado y en la que he podido confiar. Preocupada además por mi vida fuera del laboratrio, por integrarme, nunca olvidaré que las primeras montañas de los Pirineos y la primera peli en 3D las vi con ella, ;). Joaquim es también un magnífico tutor. A pesar de su juventud, es para mí un ejemplo de excelente científico, con una verdadera vocación, al cual respeto y admiro mucho. Fue la primera persona a la que vi tan emocionada con un resultado y siempre recordaré que me animaba a “disfrutar” con los experimentos y aunque al principio no lo entiendía, con el tiempo comprendí que es la mejor manera de hacer ciencia. Me ha sembrado la inquietud y provisto de herramientas para resolverlas. Me ha brindado muchísimos conocimientos y ha dedicado todo el tiempo que no tenía para guiarme. He aprendido de su carácter inspirador, optimista, persistente y muy rigoroso, pero sin perder la ternura, desde lo constructivo, siempre con modestia y humildad. Con Carme y Joaquim he tenido el privilegio de contar, con lo que para mí son los verdaderos educadores, y para resumir mi agradecimiento, como una vez les dije: “Si echara el tiempo atrás y tuviera que hacer otra vez la Tesis, los elegiría, sin duda”. Si este trabajo es también un poco de alguien más, es de Bea. Al menos así lo siento yo, porque ha estado en cada minuto de esta aventura. Unicamente ella sabrá mejor que nadie, leer entre líneas, más allá, de estas páginas. GRACIAS, por haber estado incondicionalmente a mi lado, ayudándome y soportándome, Amiga. Te agradezco infinitamente por TODO, y cuando digo TODO, es TODO, lo que me has brindado. Por siempre te estaré muy agradecida. Contigo es verdad que … “con dos que tengan el alma como de cien, con dos que se quieran bien, con dos que se quieran…basta”… No existiría esta Tesis, si no hubiera sido por ti, Omar. Me abriste la puerta y me impulsaste a volar. Me presentaste al mundo y en parte también eres responsable de este buen futuro. Siempre te estaré muy agradecida por eso.Gracias por tu mano en mis primeros pasos. Por tu gran cariño y amistad de siempre, que ha significado un gran apoyo en todo este tiempo. Gracias porque contigo me he traído una familia y un trocito de nuestra Habana. Con mucha satisfacción puedo decir, que he podido contar con amigos, que más que amigos han sido como mi familia. Albita, Nico, Hugo y Gus, a los que siempre les estaré muy agradecida, por tanto cariño, por acogerme y apoyarme cuando lo he necesitado, por tantos momentos juntos, MUCHÍSIMAS GRACIAS, sinceramente desde mi corazón. A Idalmys, amiga coterránea, gracias por tu afecto, tu apoyo, por tus mimos y cuidados. Muchas Gracias a los compañeros del laboratorio por compartir sus conocimientos. Disha, M. José, Inma, Serafí, Blanca y Pau. Agradecer a queridos amigos de otras tierras con los cuales he compartido lindos momentos: Ambika y Saravanan (por tu compañía en tantas horas extras compartidas juntos). Gracias a las fantásticas amigas Lucía y Marta. Gracias a las chicas de ElsOus Aquí: Elia, Alba y M. José La Tesis para mí no ha sido solamente este trabajo, por lo que mis agradecimientos, necesariamente deben ir más allá de estos resultados. Hay personas que aunque nunca han estado en un laboratorio me han ayudado muchísimo a vencer esta etapa. Muchas Gracias a mis amigos y primeros compañeros de piso: Arcadi, Jaume y mi queridísima Jess. Gracias a los amigos de las Remolachas Rojas, muy en especial a Martin, por su amistad, por su cariño y por complacerme con tantas canciones que me alegraban y me calmaban las nostalgias. Y por supuesto, las GRACIAS, a mi familia, en especial a mis padres, a mi tía Cusita, a mis hermanos, mis sobrinitas, mi gran amiga Idy a Frank, que han estado como siempre muy presentes, pendientes de mis pasos, apoyándome, animándome, con tantísimo cariño acercando lejanías. Gracias a la beca Jade Plus, que financia el Banco Santander, y que me otorgó la Oficina de Relaciones Internacionales de la UdL. En especial quiero agradecer a Paula Obregón, por su ayuda siempre. Espero no haber olvidado a nadie, pero de cualquier forma, quiero extender mis agradecimientos a TODOS los que de una forma u otra, directa o indirectamente me han ayudado en todo este tiempo de la Tesis.
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