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JNKVV RESEARCH JOURNAL Volume 44 Number 1 January - June 2010 Contents Research Paper Resin production system in Guggul plant [Commiphora wightii (Arnott) Bhandari] 1 Moni Thomas, Atul Shrivastava and S.S. Tomar Upland rice in Madhya Pradesh: Problems and Prospective 9 I.M. Khan, R.K. Tiwari and S.K. Rao Production, purification and characterization of phytase from Bacillus subtilis 19 Namrata Verma, Keerti Tantwai, L.P.S. Rajput, Sunil Kumar, Iti Gontia and Sharad Tiwari Production of phytase from soil isolates ofPseudomonas sp. using agricultural 25 byproduct substrates Yuvraj Soni, Keerti Tantwai, L.P.S. Rajput, Sunil Kumar, Iti Gontia and Sharad Tiwari Analgesic activity of aqueous and alcoholic extracts of Noni (Morinda citrifolia ) 31 by hot plate method Bharti Jethani, R.K.Sharma and Sudhir Kumar Jain Detection of infectious bronchitis virus in suspected post-mortem field tissue 35 samples by ELISA Sudhir K. Jain, Hemlata Jain, Megha Kadam Bedekar, Bikas Chandra Sarkhel and Sanjeev Singh Integrated nutrient management in rice wheat cropping system 39 B.M. Maurya, Jyoti Dekate and V.B. Upadhyay Evaluation of site specific nutrient management on productivity and economics 45 of rice-wheat crop sequence V.B. Upadhyay, S.K. Vishwakarma and Vikas Jain Effect of inorganic, organic and sources of nitrogen on growth and yield of 49 fodder oat (Avena sativa L.) Anay Rawat and S.B. Agrawal Response of sorghum [Sorghum bicolor (L.) Moench] cultivars to nitrogen and phosphate 53 solubilizing bacteria under different cutting intervals B.D. Ghode, Seema Bhadauria and S.D. Sawarkar Jawaharlal Nehru Krishi Vishwavidyalaya Jabalpur 482004 (Madhya Pradesh) India JNKVV RESEARCH JOURNAL ISSN : 0021-3721 Registration No.: 13-37-67 Effects of inclusion of soybean and potato on nutritional and sensory quality of 57 rice based extrudates Rajendra Singh Thakur and A.K. Tomar Extracellular enzyme profile of oyster mushrooms as affected by gamma radiation 63 Alpana Singh and Anshu Verma Screening of pesticides againstSclerotium rolfsi i causing collar rot of soybean 67 Ashish Kale and R.K. Varma Microcontroller based seed germination testing device for soybean 75 A.K. Rai and Bharati Dass Web enabled software for integrated farm production management of 79 major crops grown in central India for sustainable growth Bharati Dass and A.K. Rai Molecular characterization of BoLA-DRB3.2 allele in Nimari breed of cattle by 83 single strand conformational polymorphism (SSCP) R. Ranjan, C.D. Bhong, K.N. Chavan, S.N.S. Parmar and C.G. Joshi Information seeking behaviour of extension personnel regarding improved 87 agricultural technology N.K. Khare, A.K. Wankhade and Tapan Chandra Kar ICT based Kisan Mobile Sandesh-an innovative approach 93 Nalin Khare and Abhay Wankhade Hypsometric analysis of Kanhiya Nala watershed using 97 geographical information system S.K. Sharma, S. Tignath and U.C. Chaube Effect of modified atmosphere storage (under vacuum and nitrogen) 101 on shelf life of rice bran S.P. Srivastava and A.L. Basediya Effect of process and operational parameters of extrusion cooking of sorghum, horse 107 gram and defatted soy flour blends to produce protein rich ready-to-eat food snacks A.L. Basediya, S.P. Shrivastava and Sheela Pandey An approach for possibilities for e-preservation of library holdings 113 Siddarth Nayak, Sharad K. Jain and P.S. Nayak Published by : Dr. S.S. Tomar, Dean, Faculty ofAgriculture, JNKVV, Jabalpur 482 004 (M.P.),India Printed at : M/s Fortune Graphics, Sahu Mohalla, Near Kochhal House, Jabalpur 482 002 (M.P.) STATEMENT OF OWNERSHIP FORM IV (See Rule 8) Place of Publication : Jabalpur (Madhya Pradesh), India Periodicity of Publication : Biannual Publisher's Name : Dr. S.S. Tomar Indian Dean, Faculty of Agriculture JNKVV, Jabalpur 482 004 (M.P.), India Printer's Name : M/s Fortune Graphics & Scanning Centre Golebazar, Jabalpur 482 002 (M.P.) Editor's Name : Dr. Mohan S. Bhale Indian Senior Scientist Department of Plant Pathology JNKVV, Jabalpur 482 004 (M.P.), India Name and address of individuals : Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur Who own the news papers and partners of share holders holding more than one per cent of total capital I, S.S. Tomar, hereby declare that the particulars given above are true to the best of my knowledge and belief. Dated the 31st December, 2011 Sd/- S.S. Tomar Publisher A Publication of Jawaharlal Nehru Krishi Vishwavidyalaya Jabalpur 482 004 (Madhya Pradesh) India Phone : PBX : (+91) (0761) 2681074, 2681200 Fax : (+91) (0761) 2681074, 2681200 Website: www.jnkvv.nic.in JNKVV Research Journal Editorial Board Patron Prof. Gautam Kalloo Vice Chancellor, JNKVV, Jabalpur Chairman Dr. S.S. Tomar Dean, Faculty of Agriculture & Director Research Services, Jabalpur Members Dr. O.P. Veda Director Instruction, Jabalpur Dr. P.K. Bisen Director Extension Services, Jabalpur Dr. D.K. Mishra Dean, College of Agriculture, Jabalpur Dr. T.K. Bhattacharya Dean, College of Agricultural Engineering, Jabalpur Editor Mohan S. Bhale Co-Editor Abhishek Shukla General Information: JNKVV Research Journal is the publication of J.N. Agricultural University (JNKVV), Jabalpur for records of original research in basic and applied fields of Agriculture, Agricultural Engineering, Veterinary Science and Animal Husbandry. It is published twice a year. The journal is abstracted in CAB International abstracting system, Biological Abstracts, Indian Science Abstracts. Membership is open to all individuals and organizations coping with the mission of the University and interested in enhancing productivity, profitability and sustainability of agricultural production systems and quality of rural life through education, research and extension activities in the field of agriculture and allied sciences. Submission of manuscript for publication: Manuscripts should be submitted in duplicate to the Editor, JNKVV Research Journal, J.N. Agricultural University, Adhartal, Jabalpur 482 004 (M.P.) India. Guidelines for the authors is provided in inner pages. Membership and subscription: The annual fee for individuals is Rs. 200/- for residents in India and US$50 for residents outside India. The annual fee for Libraries and Institutions is Rs. 500/- for residents in India and US$100 for outside. All authors must be subscribers. Payment should be made by Demand Draft in favour of Dean, Faculty of Agriculture payable at Jabalpur 482 004 MP to the Editor, JNKVV Research Journal, JNKVV, Jabalpur (M.P.). Exchange of the journal: For exchange of the journal, please contact the Librarian, University Library, JNKVV, Jabalpur 482 004 (M.P.), India. ISSN : 0021-3721 Registration No. : 13-37-67 Published by : Dr. S.S. Tomar, Dean, Faculty of Agriculture, JNKVV, Jabalpur 482 004 (M.P.), India Printed at : M/s Fortune Graphics & Scanning Centre, Sahu Mohalla, Golebazar, Jabalpur (M.P.) JNKVV Res J 44(1): 1-7 (2010) Resin production system in Guggul plant [Commiphora wightii (Arnott) Bhandari] Moni Thomas, Atul Shrivastava and S.S. Tomar Directorate of Research Services Jawarharlal Nehru Krishi Vishwa Vidyalaya Jabalpur 482 004 (MP) Commiphora spp. Shrubs (Burseraceae) are native East Guggulsterone (two isomers E and Z) is an African species found in Kenya, Uganda, Tanzania, effective anti-hyperlipidemic agent obtained from the gum Ethopia, Somalia (Beentje 1994; Gillet 1991) India, resin of guggul tree, C. wightii (Dev 2006; Wang et al. Aghanisthan, Arabia and Baluchistan in Pakistan 2004; Satayavati et al. 1969; Tripathi et al. 1968; Singh (Varier1994; Atal et al.1975). Commiphora wightii L. (Syn. et al.1994). Over exploitation and slow growth of the tree Commiphora mukul Engl, Balsamodendron wightii Arn., which is associated with poor seed set, make this plant B. mukul Hook. ex stocks) belongs to the family an endangered species (Kumar et al. 2004).The Burseraceae which is a large pantropical family, forming biotechnogical production of guggulsterone has received an important element of the flora of both rain forests and attention as a promising alternative source.The plant is arid areas (Bhandari 1995). C wightii plant is known as distributed in western India, the Thar desert (Kumar and Indian bdellium is distributed in the arid, rocky tracts of Shankar 1982). Rajasthan, Gujarat, Karnataka, Rajputana, Bellari, Baluchistan, Assam, Berar and Mysore states of India, Earlier the plant was found in abundant in and Sindh and Baluchistan states of Pakistan (Varier Rajasthan, Gujarat and Madhya Pradesh but currently 1994; Atal 1975) . In Gujarat, this species is mainly found threatened because of significant declines in population in Kachchh and in some parts of Saurashtra regions (IUCN2004) and faulty extraction methods employed by (Sabnis and Rao 1983; Shah 1978). In Madhya Pradesh, traditional guggul resin collectors (Thomas et al. 2009). It it is found in Bhind, Morena, Shivpuri, Sheopur and Gwailor came in vulnerable list in the 1997 IUCN Red list as districts (Thomas et al. 2009,b). vulnerable (VU) and in 2004 data deficiency (DD) (IUCN 2004). The Government of India recently banned export Members of Burseraceae family are the source of the Guggul gum (IUCN 2004), due to its high market of well known myrrh and franchincense of the Bible (Smith price in international trade. et al. 1997). Resins from the members of the family Burseraceae play an integral role in the national, regional C. wightii plants grows to 1.0-2.5m in height and and local economies in Ethiopia, Sudan, India and have sharp spines and papery bark (Kumar and Shankar Somalia (Leminih et al. 2003; Farah 1994). Resins from 1982). Leaves have aromatic smell. The guggul gum, many woody species from African and Asian countries extracted from the stem of C. wightii. The plant is known are used commercially in the pharmaceutical, food, for its medicinal and pharmacological properties (Thomas cosmetics and perfume industries (Coates Palgrave 2002; et al. 2010 a). It controls rheumatoid arthritis, obesity Leminih et al. 2003). The term resin and gum are often and peptic ulcer (Atal et al. 1975). Hypocholestermic, used to describe any stick plant exudates which could hypolipdimic, anti-inflammatory,anti-rheumatic and anti- include a range of substances.