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National Institute of Genetics Japan NATIONAL INSTITUTE OF GENETICS JAPAN ANNUAL REPORT No. 19 1968 Published bll THE NATIONAL INSTITUTE OF GENETICS Misima. Sizuoka·ken, Japan 1969 Annual Report of the National Institute of Genetics No. 19, 1968 Published by The National Institute of Genetics, Japan 1969 CONTENTS General statement. ................................................. 1 Research member.................................................. 2 Council............................................................ 5 Projects of research for 1968....................................... 6 Researches carried out in 1968 ..................................... 9 I. Cytogenetics Scientific expedition for the study of rodents to South East Asia and Oceania. I. Members, aims and schedule of the scienti- fic expedition. YOSIDA, T. H. 9 Scientific expedition for the study of rodents to South East Asia and Oceania. II. Species and chromosome numbers of rodents collected from South East Asia and Oceania. YOSIDA, T. H., TSUCHlYA, K. and IMAI, H. T......................... 10 Scientific expedition for the study of rodents to South East Asia and Oceania. III. Chromosomal polymorphism and new karyotypes of black rat, Rattus rattus, collected in South East Asia and Oceania. YOSIDA, T. H. and TSUCHIYA, K......... 11 Scientific expedition for the study of rodents to South East Asia and Oceania. IV. Comparative idiogram analysis in several species belonging to genus Rattus collected from South East Asia and Oceania. YOSIDA, T. H. and TSUCHlYA, K......... 12 Scientific expedition for the study of rodents to South East Asia and Oceania. V. Breeding of wild rodents collected from South East Asia and Oceania. TSUCHIYA, K., YOSIDA, T. H... 13 Rat metaphase chromosomes incorporated into mouse metaphase cells. YOSIDA, T. H. and SEKIGUCHl, T...................... 15 Autoradiographic analysis of the rate of protein synthesis in diploid and tetraploid MSPC-l myeloma cells. MORIWAKI, K. and IMAI, H. T.......................................... 15 Chromosome alterations in vitro and in vivo of cultured hamster cells transformed by chemical carcinogen. SEKIYA, K...... 16 Aggregate-forming activity of rat hepatoma cells induced by DAB. KURODA, Y.......................................... 18 Pachytene pairing in a translocation heterozygote of maize hav- ing a huge knob close to the breakpoint. OHTA, Y......... 19 Preferential pairing of chromosomes in a tetraploid hybrid be- tween Oryza glaberrima and O. sativa. OKA, H. I............ 19 ii ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 19 II. Physiological and developmental genetics Differentiation of ommatidium-forming cells of Drosophila mel- anogaster in culture. KURODA, Y. .......................... 21 Effects of ecdysone analogues on differentiation of eye-antennal discs of Drosophila melanogaster in culture. KURODA, Y.... 22 The relationship between cell growth cycle and molting cycle in insect epidermis. MINATO, K. ........................... 23 Cytoplasmic male sterility and virus inoculation in Capsicum. OHTA, Y.................................................... 24 Organ differentiation of isolated Crepis leaves in vitro. YONEDA Y. 25 III. Biochemical genetics Genetic effects of DNA in Ephestia. NAWA, S. and YAMADA, M. A. 27 Further evidences of uptake of DNA by Ephestia eggs. YAMADA, M. A. and NAWA, S..................... .................... 28 Genetical and biochemical studies of lethal lemon silkworm larvae. TSUJITA, M. and SAKURAI, S........................ 29 Genetical and biochemical studies of lethal albino silkworm larvae. TSUJITA, M. and SAKURAI, S........................ 30 Preliminary examination of the cofactor role of sepiapterin for phenylalanine hydroxylase. TSUJITA, M. and SAKURAI, S.... 31 Genitical and biochemical studies of pteridine granules in hy­ podermal cells of silkworm larvae: Significance of poly- peptides inside the granules. SAKURAI, S. and TSUJITA, M. 33 Genic analysis for peroxidase isozymes in Oryza sativa and O. perennis. SHAHI, B. B., ENDo, T. and OKA, H. I. 34 Isozyme variations due to organ specificity in Oryza perennis. SHAHI, B. B., ENDo, T. and OKA, H. I. 34 Partial purification and characterization of genetically different acid phosphatase of human red cells. SHINODA, T. ......... 35 Amino acid sequences around cystine residues and COOH- terminus of rM-globulin. SHINODA, T. 36 Peroxidase isozymes in four strains of morning glory. YONEDA Y. 37 IV. Evolutionary genetics Intraspecific differentiation of cytoplasm in Aegilops caudata. KIHARA, H. and OHTA, Y. 39 Interspecific hybrid between the two species of the genus Taenia- therum of the tribe Triticeae. SAKAMOTO, S. ............... 39 Scientific expedition for the study of rodents to South East Asia and Oceania. VI. Serum transferrin polymorphism in Rattus CONTENTS iii rattus collected in South East Asia and Oceania. MORIWAKI, K., TSUCHIYA, K. and SAKATA, H. 40 Differentiation of pathogenic races of Piricularia oryzae into two groups, "Indica" and"Japonica." MORISHIMA, H........... 41 Genetic basis of the weakness of Pi plants in Oryza breviligulata and O. glaberrima. CHU, Y. E. and OKA, H. 1.............. 41 A crossing barrier isolating Oryza perennis subsp. barthii from its related taxa. CHU, Y. E. and OKA, H. 1................. 42 Variations in peroxidase, acid phosphatase and esterase isozymes of wild and cultivated Oryza species. SHAHI, B. B., MORISHIMA, H. and OKA, H. 1. .......................................... 42 V. Mathematical and statistical studies on population genetics The evolutionary rate at the molecular level. KIMURA, M...... 44 The average number of generations until fixation of a mutant gene in a finite population. KIMURA, M. and OHTA, T. ..... 44 Linkage disequilibrium due to random genetic drift. OHTA, T. and KIMURA, M............................................. 45 Rate of decrease of genetic variability in a linearly subdivided population. MARUYAMA, T.................................. 47 One-dimensional stepping stone model of finite length. MARUYAMA, T. 49 Distribution of parent-offspring distance in Mishima district. YASUDA, N. 51 VI. Experimental studies on population genetics Significance of loci of the persisting lethal genes. OSHIMA, C. and WATANABE, T. K. 53 Allelic relations between lethal genes distributed in natural populations. OSHIMA, C. and WATANABE, T. K. 54 Relationship between the dispersal of flies and the frequency of allelism of lethals in a natural population. OSHIMA, C. and WATANABE, T. K............................................ 55 Frequency of sterile chromosomes concealed in a natural popula- tion. OSHIMA, C. and WATANABE, T. K.. .................... 57 Persistence of a visible mutant in natural populations of Dro- sophila melanogaster. WATANABE, T. K. 58 VII. Radiation genetics and chemical mutagenesis in animals Repair in the mutation process studied in low and high radio- sensitivity strains of the silkworm. T AZIMA, Y............. 59 iv ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 19 Comparison of mutagenic effects of 14 MeV neutrons, r-rays and some chemical mutagens upon silkworm spermatogenic cells. T AZIMA, Y. ................................................. 60 Frequency pattern of mosaic and whole-body mutants induced by ionizing radiations in post-meiotic cells of the male silkworm. T AZIMA, Y. and ONIMARU, K..................... 61 Mutagenic action of mitomycin-C and EMS on pre-meiotic germ cells of the male silkworm. T AZIMA, Y. and ONIMARU, K... 62 Delayed mutagenic effect of mitomycin-C and EMS observed in silkworm. ONIMARU, K. and T AZIMA, Y..................... 63 The effect of post-treatment with low temperature on the fre­ quencies of radiation-induced mutation on the silkworm oocytes. MURAKAMI, A. 64 Studies on strain differences in radiosensitivity in the silkworm. VI. Further report on the screening of sensitive and re­ sistant strains to embryonic radiation killing. MURAKAMI, A. 65 Effects of X-irradiation on aggregate-forming activity and sorting-out property of HeLa cells in rotation culture. KURODA, Y. 66 Postspermatogonial rate for 14.1 MeV neutron-induced dominant mutations affecting the skeleton of mice. TUTIKAWA, K. ... 67 Lack of effect of urethane on the induction of dominant lethal mutations in male mice. TUTIKAWA, K..................... 69 VIII. Radiation genetics in microorganisms and plants A mutant of Bacillus subtilis possessing modified specificities to irradiated transforming DNA. SADAIE, Y. and KADA, T.... 71 32P-decay and mutations in Escherichia coli B/r WP2 try-. HAYASHI, M. and KADA, T. 71 Mechanisms of radio-sensitization with iodine compounds. KADA, T. and NAMIKI, M. 73 High bacteriocidal action of iodine compounds irradiated in acidic solution. KADA, T................................... 74 Action spectrum of photoreactivating light in UV irradiated maize pollen. AMANO, E. 75 Wave length effect on the photoreactivation of UV-induced mutations in maize. FUJII, T. 76 Relative biological effectiveness of 14 MeV neutrons on mutation frequency in maize. FUJII, T. 77 Fractionation experiments with gamma-rays and neutrons in maize. FUJII, Too.......................................... 78 CONTENTS v IX. Microbial genetics Determination of the length of flagella in Salmonella. IlNo. T. 80 Polarity of flagellar growth in Salmonella. IlNo, T............. 80 A method for concentrating paralyzed mutants from non- flagellated cells of Salmonella. ENOMOTO, M. ............... 81 Further studies on different frequencies of cotransduction in Salmonella.
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