FABAD

JOURNAL of PHARMACEUTICAL SCIENCES

ISSN 1300-4182 www.fabad.org.tr Volume: 37 • Issue: 3 • September 2012

An Official Journal of The Society of Pharmaceutical Sciences of Ankara (FABAD) The Society of Pharmaceutical Sciences of Ankara (FABAD) FABAD Journal of Pharmaceutical Sciences Volume: 37 Issue: 3 September 2012

Publisher Nesrin GÖKHAN KELEKÇI (Hacettepe University, Ankara, Turkey) Editor-in-Chief Didem DELIORMAN ORHAN (Gazi University, Ankara, Turkey) Associate Editors Nilüfer ORHAN (Gazi University, Ankara, Turkey) Ahmet Oğuz ADA (Ankara University, Ankara, Turkey) Aysel BERKKAN (Gazi University, Ankara, Turkey) İ. İrem ÇANKAYA (Hacettepe University, Ankara, Turkey) Editorial Board Nurettin ABACIOĞLU (Gazi University, Ankara, Turkey) Zeliha AKDEMİR (Hacettepe University, Ankara, Turkey) Asuman BOZKIR (Ankara University, Ankara, Turkey) Ünsal ÇALIŞ (Hacettepe University, Ankara, Turkey) Erdal DİNÇ (Ankara University, Ankara, Turkey) Benay Can EKE (Ankara University, Ankara, Turkey) Fatma GÜMÜŞ (Gazi University, Ankara, Turkey) İlhan GÜRBÜZ (Gazi University, Ankara, Turkey) Süeda HEKİMOĞLU (Hacettepe University, Ankara, Turkey) Blanca LAFFON (University of A Coruña, A Coruna, Spain) Levent ÖNER (Hacettepe University, Ankara, Turkey) A. Yekta ÖZER (Hacettepe University, Ankara, Turkey) Sibel ÖZKAN (Ankara University, Ankara, Turkey) Yalçın ÖZKAN (Gulhane Military Medical Academy, Ankara, Turkey) Tanju ÖZÇELİKAY (Ankara University, Ankara, Turkey) Nurten ÖZDEMİR (Ankara University, Ankara, Turkey) Rita De PASQUALE (University of Messina, Messina, Italy) Nadja Souza PINTO (University of Sao Paulo, Sao Paula, Brazil) Selma SARAÇ (Hacettepe University, Ankara, Turkey) Sevgi ŞAR (Ankara University, Ankara, Turkey) Uğur TAMER (Gazi University, Ankara, Turkey) Maria Fernanda TAVIANO (University of Messina, Messina, Italy) João Paulo TEIXEIRA (National Institute of Health, Porto, Portekiz) Meral TUNÇBİLEK (Ankara University, Ankara, Turkey) Nilüfer TURAN (Gazi University, Ankara, Turkey) Eva Maria Munoz VALENTINE (Univarsidade de Santiago de Compostela, Spain) Beata WALCZAK (University of Silesia, Poland) Sulhiye YILDIZ (Ankara University, Ankara, Turkey) Serhat Ali ARIKAN (Language Editor, Ankara, Turkey)

The FABAD Journal of Pharmaceutical Sciences is published quarterly by the Society of Pharmaceutical Sciences of Ankara (FABAD) All expressions of opinion and statements of supposed facts appearing in articles and / or advertisiments carried in this journal are published on the responsibility of the author and / or advertiser, and are not to be regarded those of the So- ciety of Pharmaceutical Sciences of Ankara. The manuscript submitted to the Journal has the requirement of not being published previously and has not been submitted elsewhere. Manuscript should bu prepared in accordance with the requirements specified as in the back cover. The submission of the manuscript to the Journal is not a condition for accep- tance; articles are accepted or rejected on merit alone. The Journal is distributed to the members only. Nonmembers can obtain the journal by a donation to FABAD. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems without written permission from FABAD Journal of Pharmaceutical Sciences.

The FABAD Journal of Pharmaceutical Sciences is indexed in Chemical Abstracts, Analytical Abstracts, International Pharmaceutical Abstracts, Excerpta Medica (EMBASE), ISI Master Journal List, Scopus and Proquest, Akademik Dizin All manuscripts and editorial correspondences should be sent via e-mail to Didem Deliorman Orhan (Editor-in-Chief): [email protected] The payment of donation has to be sent to the bank Account Number given below: Türkiye İş Bankası, Gazi Mahallesi Şubesi, Ankara, Account number: 4286 304400 201434

CONTENTS Research Articles

123 Antioxidant Capacity and Total Phenol Contents of Bifora radians Bieb. Ayşegül KÖROĞLU, Muhammed Mesud HÜRKUL, Özen ÖZBAY

129 The Relationship Between Cadmium Contents of Tobacco and Their Ash in Cigarettes Marketed Under Different Brands Beril KADIOĞLU, Esin NALTEKIN, Mohammad CHAREHSAZ, Hande SIPAHI, Buğra SOYKUT, Ahmet AYDIN

133 A Validated RP-HPLC Method for the Determination of Bendamustine hydrochloride in Tablet Dosage Form using Gemcitabine hydrochloride as Internal Standard Kiran Kumar KATARE, Venkata Nadh RATNAKARAM, Nagoji K.E.V.

141 Spectrophotometric Assay of Diethylcarbamazine citrate using Folin-Ciocalteu Phenol Reagent Nagaraju SWAMY, Kudige Nagaraj PRAHANTH, Kanakapura BASAVAIAH, Kanakapura BASAVAIAH VINAY

151 Analgesic and antioxidant activity of some Echium species wild growing in Turkey Nuraniye ERUYGUR, Gülderen YILMAZ, Osman ÜSTÜN

161 Antioxidant capacities, ascorbic acid and total phenol contents of the plants sold as Rose hip in Turkey Didem DELIORMAN ORHAN, Özlem ÖZLÜK, Sanem HOŞBAŞ COŞKUN

INSTRUCTIONS FOR AUTHORS All new manuscripts and correspondence regarding editorial matters should be addressed to the Editor-in-Chief, Prof. Dr. Didem Deliorman Orhan Prof.Dr. Didem Deliorman Orhan FABAD J Pharm Sci Editor-in-Chief Phone: +90 3122023173 Fax: +90 312 2235018 E-mail: [email protected] SCOPE OF THE JOURNAL The journal publishes original, innovative research articles, review articles, short communications, scientific reports in all fields of pharmaceutical and related health sciences. It is recommended that all articles do not exceed a total of 15 double spaced pages including references, tables, figure legends and figures using font size 12. Review articles are allowed up to 25 pages. The publication language is English. CONFLICT OF INTEREST All authors are requested to disclose any actual or potential conflict of interest including any financial, personal or other relationships with other people or organizations within three years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work. DECLARATION OF SUBMISSION Submission of an article implies that the work described has not been published previously (except in the form of an abstract or as part of a pub- lished lecture or academic thesis), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, without the written consent of the copyright-holder. SUBMISSION Submission to this journal proceeds only by e-mail to the editor ([email protected]). All correspondences, including notification of the Editor’s decision and requests for revision takes place by e-mail. Research Articles Articles in all fields of pharmaceutical and related health sciences (physical, chemical, biological, medical, dental, social, etc.), presenting an origi- nal research could be consider for publication. Scientific Reviews An updated comprehensive review, with sufficient number of scientific articles including original paper(s) of the author(s) on a subject, evaluating and discussing the results could be consider for publication. Short Communications Rapid announcement of the results of a continuing research, not exceeding two printed pages can be submitted as a short communication. Scientific Reports a) Articles dealing with important scientific pharmaceutical events in Turkey and in the world, b) Recent equipment and materials, c) Short information and reports on the pharmaceutical congresses, conferences, symposia, etc., d) English summaries of Ph.D. theses in pharmaceutical sciences, e) Reviews of scientific books in pharmaceutical sciences, f ) Reviews on recently published scientific journals, etc. can be submitted as a short communication. Preparation of manuscripts 1. Text should be typed double spaced in A4 format with a margin of 2 cm on each side of the page using a word processor, MS Office for Win- dows. 2. Following the article title, author names and affiliations should be stated. All affiliations should be stated with a lower-case superscript letter. Provide the full postal address of each affiliation, including the country name, and e-mail address of the corresponding author. 3. A cover page should be provided with the title of article, author name and affiliations (including fax, telephone, e-mail addresses) of the author to whom all correspondence should be directed. Manuscripts submitted should have a separate letter including the signatures of each author. 4. The Declaration of Helsinki as amended in Seoul 2008 for humans, and the European Community guidelines and Turkish Official guidelines as accepted principles for the use of experimental animals, must be also adhered to. 5. Sections of the manuscripts should adhere to the following sequence: Summary It should be written in Turkish and English, not exceeding 200 words, title and keywords should be given in both languages. List of keywords (max- imum 5) should be provided under the summaries. The aim of the study, methods, results and conclusion should be described briefly. Introduction The objective of the work should be stated and an adequate background provided, avoiding a detailed literature survey or a summary of the results. Material and methods Sufficient detail should be provided to allow the work to be reproduced. Methods already published should be indicated by a reference: only rele- vant modifications should be described. Results Results should be clear and concise. Discussion This should explore the significance of the results of the work, not repeat them. A combined Results and Discussion section is often appropriate. Avoid extensive citations and discussion of published literature. Conclusions The main conclusions of the study may be presented in a short Conclusions section, which may stand alone or form a subsection of a Discussion or Results and Discussion section. References The reference numbers should be given in parentheses. If more than one reference is cited at a time, they should be listed by date, starting with the earliest. Articles: 1. Stalcup AM, Chang SC, Armstrong DW, Pitha J. (s)-2-Hydroxypropyl-ßcyclodextrin, a new chiral stationary phase for reversed–phase liquid chromatography. J Chromatogr A 513: 181-194, 1990. Books: 2. Van de Waterbeemd H. Quantitative approaches to structure-activity relationships. In: Wermuth CG, editor. The practice of medicinal chemistry. 4th ed. Great Britain: Academic Press; 2001. p. 367-89. Watson, DG. Pharmaceutical analysis. 1st ed. Chine: Churchill Livingstone; 1999. Dissertations: 3. Karakurt A. Studies on synthesis, enantiomeric separation, anticonvulsant and antimicrobial activities of some new 2-acetylnaphthalene deriva- tives [dissertation]. Ankara (Turkey): Hacettepe University Institute of Health Sciences; 2004. Patents: 4. Larsen CE, Trip R, Johnson CR, inventors; Novoste Corporation, assignee. Methods for procedures related to the electrophysiology of the heart. US patent 5,529,067. 1995 Jun 25. Electronic articles: Morse SS. Factors in the emergence of infectious diseases. Emerg Infect Dis {serial online] 1995 Jan-Mar [cited 1996 Jun 5]; 1(1):[24 screens]. Available from: URL: http://www.cdc.gov/ncidod/EID/eid.htm Figures and graphics Figures, graphics and photographs should be in TIFF or JPEG format submitted as a separate file. Figure legends should be written on a separate page. Tables Tables should be submitted on a separate page.