DOCSLIB.ORG

2014 Annual Meeting Abstracts

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
2014 Annual Meeting Abstracts Poultry Science Association 103rd Annual Meeting Abstracts To be presented July 14–17, 2014 Corpus Christi, Texas Also contains abstracts from the 2014 International Poultry Scientific Forum January 27–28, 2014 EDITOR-IN-CHIEF ® T. E. Porter (2016) POULTRY SCIENCE SECTION EDITORS ASSOCIATE EDITORS (2013–2014) Environment, Well-Being, N. Acar (2015) I. Hanning (2016) M. Pines (2014) and Behavior O. Adeola (2015) W. Huff (2014) T. Poole (2016) I. Estevez (2014) H. Ahmadi (2013) R. M. Hulet (2014) R. Poureslami (2015) M. M. Beck (2016) W. Alali (2016) M. Hume (2015) A. Pradhan (2014) T. J. Applegate (2015) A. Jackson-Davis (2016) G.-H. Qi (2015) Genetics C. Ashwell (2016) D. Jackwood (2014) G. Rajashekara (2014) J. Dodgson (2016) D. Babu (2015) P. A. Johnson (2016) V. Ravindran (2015) Immunology, Health, K. Bafundo (2016) C. Jones (2016) K. Reed (2016) and Disease M. R. Bakst (2014) P. Kaiser (2014) T. B. Rodenburg (2014) R. L. Taylor Jr. (2016) R. Beckstead (2016) I. Kang (2015) G. J. M. Rosa (2016) B. R. Behrends (2016) N. Kansaku (2016) W. B. Roush (2013) Metabolism and Nutrition L. Berghman (2014) E. Kebreab (2013) I. Rozenboim (2014) G. Cherian (2014) W. Berry (2014) E. J. Kim (2016) C. Ruiz-Feria (2013) M. Rodehutscord (2016) D. Biswas (2014) W. Kim (2016) S. M. Rutherfurd (2015) E. Esteve-Garcia (2014) J. Brake (2013) M. Koci (2015) M. Schilling (2015) K. Bregendahl (2014) K. W. Koelkebeck (2013) C. Schmidt (2016) Molecular, Cellular, B. Brehm-Stecher (2016) M. H. Kogut (2014) P. Selle (2013) and Developmental Biology J. Buyse (2014) A. Kollanoor-Johny (2016) R. K. Selvaraj (2015) G. Bedecarrats (2016) D. Caldwell (2014) B.-W. Kong (2013) D. H. Shah (2016) Physiology, Endocrinology, F. Carbonero (2016) O.-K. Koo (2015) S. Sharif (2015) and Reproduction H. Cervantes (2014) E. Koutsos (2015) C. S. Sharma (2015) S. C. Chapman (2016) W. Kuenzel (2014) K. Shimada (2016) W. Bott je (2015) H.-W. Cheng (2016) I. Kyriazakis (2015) F. Siewerdt (2016) A. L. Johnson (2014) K. K. Chousalkar (2013) S. J. Lamont (2014) M. Singh (2014) Processing, Products, S. Cloutier (2016) J. D. Latshaw (2015) B. Svihus (2014) and Food Safety A. Collin (2014) J. Lee (2013) C. Swaggerty (2016) C. M. Owens (2014) E. Collisson (2014) E. Leone (2016) S. Tesseraud (2014) S. C. Ricke (2016) M. Compton (2015) F. C. Leung (2016) L. Thompson (2016) M. E. Cook (2014) H. Lillehoj (2015) K. Tona (2014) Production, Modeling, P. Cott er (2014) J. Lin (2015) K. Turner (2014) and Education C. Coufal (2013) H.-C. Liu (2016) H. van den Brand (2014) A. McElroy (2016) P. A. Curtis (2013) K. Maciorowski (2014) J. van der Poel (2015) E. Delezie (2014) R. H. Marin (2016) S. Velleman (2015) Contemporary Issues R. Dennis (2014) A. McElroy (2016) R. Walzem (2014) T. E. Porter (2016) J. Donaldson (2015) S. R. McKee (2015) F. Weber (2015) OFFICERS W. A. Dozier III (2015) A. Meluzzi (2015) B. Werner (2014) C. Dunkley (2016) K. Mertens (2013) H. Willemsen (2014) President K. D. Dunkley (2014) S. Mignon-Grasteau (2014) S. K. Williams (2015) M. O. Smith R. G. Elkin (2015) S. Milillo (2014) A. Witt ers (2014) First Vice President D. Emmerson (2015) M. T. Musgrove (2014) E. A. Wong (2016) T. J. Applegate G. Erf (2015) I. A. Naas (2016) M. Yegani (2016) J. Evans (2016) R. Nannapaneni (2016) A. Yersin (2014) Second Vice President N. Everaert (2014) A. Narcy (2015) D. Zadworny (2016) D. J. Caldwell M. Farnell (2016) K. J. Navara (2014) M. Zeman (2014) Secretary-Treasurer S. Foley (2016) R. Nayak (2016) J. Zentek (2016) R. M. Hulet R. Forder (2015) S. L. Noll (2016) W. Zhai (2015) N. French (2014) S. F. O’Keefe (2016) H. Zhang (2016) Past President J. Fulton (2014) R. Okimoto (2016) X. Zhang (2013) M. T. Kidd R. Gast (2014) O. Onagbesan (2014) X. Zhao (2015) Directors K. E. Gibson (2015) H. Parmentier (2014) H. Zhou (2016) A. P. McElroy (2014) E. Gilbert (2015) R. L. Payne (2015) H. Zhuang (2015) I. Gimeno (2016) A. J. Pescatore (2014) I. Zulkifl i (2014) D. R. McIntyre (2014) H. Guo (2016) T. Pharr (2016) B. D. Fairchild (2015) D. Korver (2015) FASS Publications Staff Chris Davies Ron Keller J. B. Hess (2016) [email protected] Sharon Frick Lisa Krohn S. R. Fernandez (2016) Susan Pollock, Managing Editor Gayle Gleichman Shauna Miller Louise Adam Armgard Haken Student Representatives Jorge Cazares Christine Horger K. A. Byrne (2014) A. J. Calvert (2015) Poultry Science® (ISSN 0032-5791) is published 12 times per year (monthly). Periodicals Postage Paid at Cary, NC, and additional mailing offi ces. POSTMASTER: Send address changes to Poultry Science, Journals Customer Service Department, Oxford University Press, 2001 Evans Road, Cary, NC 27513-2009. S R Y C Subscription information Annually for North America, $589 (electronic), $647 (print), or $681 (electronic and T I E L N U print). Annually for Rest of World, £380/€467 (electronic), £438/€539 (print), or £461/€567 (electronic and print); C O E P single copies are $45. All inquiries about subscriptions should be sent to Journals Customer Service Department, Oxford Journals, Great Clarendon Street, Oxford OX2 6DP, UK, Tel: +44 (0) 1865-35-3907, e-mail: jnls.cust.serv@oup. com. In the Americas, please contact Journals Customer Service Department, Oxford Journals, 2001 Evans Road, A 1190908 Cary, NC 27513. Tel: 800-852-7323 (toll-free in USA/Canada) or 919-677-0977, e-mail: [email protected]. S N S O O C I AT I The PSA membership fee for individuals is $120 and includes electronic access to Poultry Science. A print subscrip- tion for PSA members is available for $80 (US) or $107 (Rest of World). All inquiries about membership should be POULTRY SCIENCE sent to Poultry Science Association Inc., 1800 S. Oak Street, Suite 100, Champaign, IL 61820-6974, Tel: 217-356-5285, ASSOCIATION INC. e-mail: [email protected]. Organized 1908 Claims Publisher must be notifi ed of claims within four months of dispatch/order date (whichever is later). Sub- htt p://www.poultryscience.org scriptions in the EEC may be subject to European VAT. Claims should be made to Poultry Science, Journals Customer htt p://ps.oxfordjournals.org Service Department, Oxford University Press, 2001 Evans Road, Cary, NC 27513, Tel: 800-852-7323 (toll-free in USA/ Canada) or 919-677-0977, e-mail: [email protected]. ©2014 Poultry Science Association Inc. A journal of the Poultry Science Association published by Oxford University Press. Abstracts of the Poultry Science Association Annual Meeting SYMPOSIA AND ORAL SESSIONS Monday, July 14, 2014 Abstract Page No. No. Student Competition: Behavior, Extension, Environment, and Management .....................................................................................................................1–11 1 Student Competition: Genetics, Immunology, Pathology ....................................................................................................................................................12–23 5 Student Competition: Nutrition I .........................................................................................................................................................................................24–35 9 Student Competition: Nutrition II ......................................................................................................................................................................................36–46 13 Student Competition: Nutrition III .....................................................................................................................................................................................47–60 17 Student Competition: Nutrition IV ....................................................................................................................................................................................61–74 22 Student Competition: Physiology, Endocrinology, and Reproduction ..............................................................................................................................75–89 27 Student Competition: Processing, Products, and Food Safety ...........................................................................................................................................90–101 32 Student Competition: Intestinal Health ..............................................................................................................................................................................102–109 36 Immunology .......................................................................................................................................................................................................................110–114 39 Pathology ...........................................................................................................................................................................................................................115–123 41 Genetics ..............................................................................................................................................................................................................................124–127 44 Tuesday, July 15, 2014 Metabolism and Nutrition–Amino Acids ...........................................................................................................................................................................128–138 45 Metabolism and Nutrition–Nutrition I ...............................................................................................................................................................................139–152
Load more

Recommended publications
  • Phytic Acid (Phytate)/ Total Phosphorus
    www.megazyme.com PHYTIC ACID (PHYTATE)/ TOTAL PHOSPHORUS Measured as phosphorus released by phytase and alkaline phosphatase ASSAY PROCEDURE K-PHYT 05/19 (50 Assays per Kit) © Megazyme 2019 INTRODUCTION: Phytic acid (phytate; myo-inositol 1,2,3,4,5,6-hexakisphosphate) is the primary source of inositol and storage phosphorus in plant seeds contributing ~ 70% of total phosphorus. The abundance of phytic acid in cereal grains is a concern in the foods and animal feeds industries because the phosphorus in this form is unavailable to monogastric animals due to a lack of endogenous phytases; enzymes specific for the dephosphorylation of phytic acid. In addition, the strong chelating characteristic of phytic acid reduces the bioavailability of other essential dietary nutrients such as minerals (e.g. Ca2+, Zn2+, Mg2+, Mn2+, Fe2+/3+), proteins and amino acids.2 High phytic acid content feeds are generally supplemented with inorganic phosphate, however this causes increased faecal phosphate levels and subsequent eutrophication of waterways. Alternatively, supplementation with commercial phytases is becoming increasingly popular and reduces the requirement for inorganic phosphate supplementation as well as the associated environmental issues. Currently, there is no commercially available, simple, quantitative method for phytic acid and, while such measurement is relatively complex, the generally accepted AOAC Method 986.11 has limitations.3 For each individual analysis the method requires cumbersome anion-exchange purification and a major inherent assumption here is that only phytic acid is purified. While this assumption is viable for non-processed grains for which phytic acid comprises at least 97% of total inositol phosphates, it is not viable for processed foods and feeds which can contain higher levels of some lower myo-inositol phosphate forms (i.e.
    [Show full text]
  • Effect of Ph and Temperature on the Activity of Phytase Products Used In
    Brazilian Journal of Poultry Science Revista Brasileira de Ciência Avícola Effect of ph and Temperature on the Activity of ISSN 1516-635X Jul - Sept 2012/ v.14 / n.3 / 159-232 Phytase Products Used in Broiler Nutrition Author(s) ABSTRACT Naves L de P1 Corrêa AD2 The activity of three commercial microbial phytase (Aspergillus Bertechini AG3 oryzae, A. niger, and Saccharomyces cerevisae) products used in broiler Gomide EM4 Santos CD dos2 nutrition was determined at different pH (2.0 to 9.0) and temperature (20 to 90°C) values. Enzymatic activity was determined according to the reaction of the phytase with its substrate (sodium phytate), in four replicates, and was expressed in units of phytase activity (FTU). A. oryzae phytase exhibited optimal activity at pH 4.0 and 40°C, but 1Graduate student in Monogastric Nutrition of its absolute activity was the lowest of the three phytases evaluated. the Animal Science Department − Federal A. niger phytase exhibited maximal activity close to pH 5.0 and 45oC, University of Lavras (UFLA). whereas S. cerevisae phytase presented its highest activity at pH close to 2Professor of the Chemistry Department/ UFLA. 4.5 and temperatures ranging between 50 and 60°C. It was concluded 3Professor of the Animal Science Department/ that A. niger and S. cerevisae phytase products exhibited the highest UFLA. absolute activities in vitro at pH and temperature values (pH lower than 4Ph. D. student in Monogastric Nutrition of o the Animal Science Department/UFLA. 5.0 and 41 C) corresponding to the ideal physiological conditions of broilers, which would theoretically allow high hydrolysis rate of the phytate contained in the feed.
    [Show full text]
  • Peraturan Badan Pengawas Obat Dan Makanan Nomor 28 Tahun 2019 Tentang Bahan Penolong Dalam Pengolahan Pangan
    BADAN PENGAWAS OBAT DAN MAKANAN REPUBLIK INDONESIA PERATURAN BADAN PENGAWAS OBAT DAN MAKANAN NOMOR 28 TAHUN 2019 TENTANG BAHAN PENOLONG DALAM PENGOLAHAN PANGAN DENGAN RAHMAT TUHAN YANG MAHA ESA KEPALA BADAN PENGAWAS OBAT DAN MAKANAN, Menimbang : a. bahwa masyarakat perlu dilindungi dari penggunaan bahan penolong yang tidak memenuhi persyaratan kesehatan; b. bahwa pengaturan terhadap Bahan Penolong dalam Peraturan Kepala Badan Pengawas Obat dan Makanan Nomor 10 Tahun 2016 tentang Penggunaan Bahan Penolong Golongan Enzim dan Golongan Penjerap Enzim dalam Pengolahan Pangan dan Peraturan Kepala Badan Pengawas Obat dan Makanan Nomor 7 Tahun 2015 tentang Penggunaan Amonium Sulfat sebagai Bahan Penolong dalam Proses Pengolahan Nata de Coco sudah tidak sesuai dengan kebutuhan hukum serta perkembangan ilmu pengetahuan dan teknologi sehingga perlu diganti; c. bahwa berdasarkan pertimbangan sebagaimana dimaksud dalam huruf a dan huruf b, perlu menetapkan Peraturan Badan Pengawas Obat dan Makanan tentang Bahan Penolong dalam Pengolahan Pangan; -2- Mengingat : 1. Undang-Undang Nomor 18 Tahun 2012 tentang Pangan (Lembaran Negara Republik Indonesia Tahun 2012 Nomor 227, Tambahan Lembaran Negara Republik Indonesia Nomor 5360); 2. Peraturan Pemerintah Nomor 28 Tahun 2004 tentang Keamanan, Mutu dan Gizi Pangan (Lembaran Negara Republik Indonesia Tahun 2004 Nomor 107, Tambahan Lembaran Negara Republik Indonesia Nomor 4424); 3. Peraturan Presiden Nomor 80 Tahun 2017 tentang Badan Pengawas Obat dan Makanan (Lembaran Negara Republik Indonesia Tahun 2017 Nomor 180); 4. Peraturan Badan Pengawas Obat dan Makanan Nomor 12 Tahun 2018 tentang Organisasi dan Tata Kerja Unit Pelaksana Teknis di Lingkungan Badan Pengawas Obat dan Makanan (Berita Negara Republik Indonesia Tahun 2018 Nomor 784); MEMUTUSKAN: Menetapkan : PERATURAN BADAN PENGAWAS OBAT DAN MAKANAN TENTANG BAHAN PENOLONG DALAM PENGOLAHAN PANGAN.
    [Show full text]
  • Final Dilution in Petri Plates with the Experiment, the Birds Were Weighed on Day-Of-Hatch and D 7, 10, Proper Culture Media, Tryptic Soy Agar (Biokar)
    Symposium on Gut Health in Production of Food Animals November 10–12, 2014, St. Louis, Missouri Program and Abstracts www.GutHealthSymposium.com/2014 SYMPOSIUM ON GUT HEALTH IN PRODUCTION OF FOOD ANIMALS CONTENTS Welcome ............................................................................................................................3 Map ....................................................................................................................................4 Program .............................................................................................................................5 Poster Presentations......................................................................................................10 Abstracts .........................................................................................................................12 Poster Abstracts .............................................................................................................26 1 SYMPOSIUM ON GUT HEALTH IN PRODUCTION OF FOOD ANIMALS WELCOME On behalf of the Organizing Committee for the 3rd Symposium on Gut Health in Production of Food Animals, I welcome you to St. Louis, Missouri! I hope that the change in venue has made travel to the symposium easier and less expensive. Like the first two symposia organized around the topic of gut health in food animals, the aim this year is to bring together a group of scientists from academia, government, and industry to discuss the role of gut health in animal production and the essential
    [Show full text]
  • <I>AUREOCOCCUS ANOPHAGEFFERENS</I>
    University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2016 MOLECULAR AND ECOLOGICAL ASPECTS OF THE INTERACTIONS BETWEEN AUREOCOCCUS ANOPHAGEFFERENS AND ITS GIANT VIRUS Mohammad Moniruzzaman University of Tennessee, Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Environmental Microbiology and Microbial Ecology Commons Recommended Citation Moniruzzaman, Mohammad, "MOLECULAR AND ECOLOGICAL ASPECTS OF THE INTERACTIONS BETWEEN AUREOCOCCUS ANOPHAGEFFERENS AND ITS GIANT VIRUS. " PhD diss., University of Tennessee, 2016. https://trace.tennessee.edu/utk_graddiss/4152 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Mohammad Moniruzzaman entitled "MOLECULAR AND ECOLOGICAL ASPECTS OF THE INTERACTIONS BETWEEN AUREOCOCCUS ANOPHAGEFFERENS AND ITS GIANT VIRUS." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, with a major in Microbiology. Steven W. Wilhelm, Major Professor We have read this dissertation
    [Show full text]
  • Solarbio Catalogue with PRICES
    CAS Name Grade Purity Biochemical Reagent Biochemical Reagent 75621-03-3 C8390-1 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonateCHAPS Ultra Pure Grade 1g 75621-03-3 C8390-5 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonateCHAPS 5g 57-09-0 C8440-25 Cetyl-trimethyl Ammonium Bromide CTAB High Pure Grade ≥99.0% 25g 57-09-0 C8440-100 Cetyl-trimethyl Ammonium Bromide CTAB High Pure Grade ≥99.0% 100g 57-09-0 C8440-500 Cetyl-trimethyl Ammonium Bromide CTAB High Pure Grade ≥99.0% 500g E1170-100 0.5M EDTA (PH8.0) 100ml E1170-500 0.5M EDTA (PH8.0) 500ml 6381-92-6 E8030-100 EDTA disodium salt dihydrate EDTA Na2 Biotechnology Grade ≥99.0% 100g 6381-92-6 E8030-500 EDTA disodium salt dihydrate EDTA Na2 Biotechnology Grade ≥99.0% 500g 6381-92-6 E8030-1000 EDTA disodium salt dihydrate EDTA Na2 Biotechnology Grade ≥99.0% 1kg 6381-92-6 E8030-5000 EDTA disodium salt dihydrate EDTA Na2 Biotechnology Grade ≥99.0% 5kg 60-00-4 E8040-100 Ethylenediaminetetraacetic acid EDTA Ultra Pure Grade ≥99.5% 100g 60-00-4 E8040-500 Ethylenediaminetetraacetic acid EDTA Ultra Pure Grade ≥99.5% 500g 60-00-4 E8040-1000 Ethylenediaminetetraacetic acid EDTA Ultra Pure Grade ≥99.5% 1kg 67-42-5 E8050-5 Ethylene glycol-bis(2-aminoethylether)-N,N,NEGTA′,N′-tetraacetic acid Ultra Pure Grade ≥97.0% 5g 67-42-5 E8050-10 Ethylene glycol-bis(2-aminoethylether)-N,N,NEGTA′,N′-tetraacetic acid Ultra Pure Grade ≥97.0% 10g 50-01-1 G8070-100 Guanidine Hydrochloride Guanidine HCl ≥98.0%(AT) 100g 50-01-1 G8070-500 Guanidine Hydrochloride Guanidine HCl ≥98.0%(AT) 500g 56-81-5
    [Show full text]
  • Phosphodiesterases
    ^ ^ ^ ^ I ^ ^ ^ ^ II ^ II ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ I ^ � European Patent Office Office europeen des brevets EP 0 967 284 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) |nt CI* C12N 15/55, C12N 9/16, 29.12.1999 Bulletin 1999/52 qq-) N 33/68, C12Q 1/44, C07 K 1 6/40 C 1 2 N 1 5/1 1 v(21)to i ;\ Appa hcation, ♦■ number:k 99303985.8QQinioQc fi ,' ,' A61 K 38/46, A61 K 35/00, (22) Date of filing: 21.05.1999 A61 K 48/00 (84) Designated Contracting States: (72) Inventors: AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU • Lanfear, Jeremy, c/o Pfizer Central Research MC NL PT SE Sandwich, Kent CT13 9NJ (GB) Designated Extension States: • Robas, Nicola Melanie, AL LT LV MK RO SI c/o Pfizer Central Research Sandwich, Kent CT13 9NJ (GB) (30) Priority: 28.05.1998 GB 9811500 30.10.1998 GB 9823882 (74) Representative: Harding, Charles Thomas et al 04.12.1998 GB 9826777 D. Young & Co. 09.04.1999 GB 9908247 21 New Fetter Lane 10.05.1999 GB9910801 London EC4A 1 DA (GB) (83) Declaration under Rule 28(4) EPC (expert Remarks: solution) The applicant has subsequently filed a sequence listing and declared, that it includes no new matter. (71) Applicants: • Pfizer Limited Sandwich Kent CT13 9NJ (GB) Designated Contracting States: GB • PFIZER INC. Groton, CT 06340 (US) Designated Contracting States: BE CH DE DK ES FI FR GR IE IT LI LU MC PT SE AT CY (54) Phosphodiesterases (57) Amino acid sequences and nucleotide se- SEQ ID No.
    [Show full text]
  • Phytase and Other Phosphorus Reducing Feed Ingredients
    Feed Management Phytase and Other Phosphorus Reducing Feed Ingredients by Todd J. Applegate and Brian Richert - Purdue University A Key Ingredient in Livestock Roselina Angel, University of Maryland, and Poultry Nutrient Management College Park Disclaimer This fact sheet reflects the best available information Introduction on the topic as of the publication date. This fact sheet has been developed to support Date 6-18-2008 the implementation of the Natural Resources Conservation Service Feed Management 592 Practice This Feed Management Standard. The Feed Management 592 Practice Education Project was Standard was adopted by NRCS in 2003 as another funded by the USDA tool to assist with addressing resource concerns on NRCS CIG program. livestock and poultry operations. Feed management Additional information can assist with reducing the import of nutrients to the can be found at farm and reduce the excretion of nutrients in manure. http://www.puyallup.wsu. edu/dairy/nutrient- The primary constituents of diets for poultry management/default.asp and swine are plant-based ingredients which come primarily from the seeds of plants. Most of the stored This project is affiliated phosphorus (P) in plants is found in seeds mainly as a with the Livestock & component of a molecule called phytin. Phytin-P is Poultry Environmental poorly available to poultry and swine, and this Learning Center - availability varies both within and among ingredients. http://www.extension.org/ The enzyme phytase releases phosphate groups from animal+manure+managem phytin potentially making this released P available to ent. the animal, thereby reducing P excreted from poultry and swine by 15 to 30%.
    [Show full text]
  • Fingerprint Motifs of Phytases
    African Journal of Biotechnology Vol. 12(10), pp. 1138-1147, 6 March, 2013 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB12.1279 ISSN 1684–5315 ©2013 Academic Journals Full Length Research Paper Fingerprint motifs of phytases Cheng-Ming Fan1#, Ya-Hui Wang2#, Chang-Ying Zheng2 and Yong-Fu Fu1* 1MOA Key Lab of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing 100081, China. 2College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, China. Accepted 2 October, 2012 Phytate is the major form of phosphorus storage in plant seeds and the soil organic phosphorus. The phytase is a class of enzymes which catalyzes the hydrolysis of phytate and release free orthophosphoric acid. Motifs provide insight into protein structure, function and evolution. 39 fingerprint motifs were obtained in phytases through multiple EM for motif elicitation (MEME) analysis based on 54 whole phytase sequences with an average of 16.6% identity (ranging from 3.9 to 52.5%). The phytase family is classified into seven groups: HAPhys, PAPhys, BPPhys, BPRPhys, PTPhys, ALPhys and APPAs according to the phylogenetic analysis and motif characters. Every phytase group has its typical fingerprint motifs through motif alignment and search tool (MAST) results. Based on the phylogenetic tree and characteristics of phytase motif organisations, BPPhy family has 13 motifs, and is classified into five subgroups, BPPhy I to V. HAPhy with 11 motifs is classified into four subgroups, HAPhyI to IV. PAPhy, BPRPhy, PTPhy and APPAs have four, four, four and six fingerprint motifs, respectively, but no fingerprint motifs are found by MAST for ALPhys.
    [Show full text]
  • Adaptive Increase in Phytate Digestibility by Phosphorus
    Downloaded from Br. J. Nutr. (1983), 49, 145 145 https://www.cambridge.org/core Adaptive increase in phytate digestibility by phosphorus-deprived rats and the relationship of intestinal phytase (EC 3.1.3.8)and alkaline phosphatase (EC 3.1.3.1)to phytate utilization BY ROBERT J. MOORE* AND TRYGVE L. VEUMt 110 Animal Science Research Center, University of Missouri, Columbia, . IP address: Missouri 65211, USA (Received I June 1982- Accepted 2 September 1982) 170.106.40.40 1. The effects of phosphorus deprivation on phytate digestibility, phosphorus utilization and intestinal phytase (EC 3.1 .3.8) and alkaline phosphatase (EC 3.1 .3.1) in rats were investigated. 2. P deprivation was achieved by giving rats a diet containing 3 g P/kg and resulted in hypophosphataemia, hypercalcaemia, hypercalciuria, and lower levels of P absorbed and retained, and calcium retained. , on 3. Rats adapted to P deprivation by increasing the digestion of total dietary-P and phytate-P. 01 Oct 2021 at 22:42:35 4. Levels of intestinal alkaline phosphatase and alkaline phytase were not different between the two treatment groups. 5. P deprivation in the rats given the marginal-P diet may be a resu!t of a lower absorption of total dietary-P or increased absorption of inositol phosphates formed during the enzymic hydrolysis of phytate which are not readily utilized by the rat. 6. These results suggest that intestinal phytase and alkaline phosphatase do not play a role in the adaptive increase in phytate digestibility by rats given marginal-P diets. The adaptation may result from enhanced phytase , subject to the Cambridge Core terms of use, available at or alkaline phosphatase synthesis by the gastrointestinal microflora stimulated by a lower level of P in the digesta.
    [Show full text]
  • Generate Metabolic Map Poster
    Authors: Zheng Zhao, Delft University of Technology Marcel A. van den Broek, Delft University of Technology S. Aljoscha Wahl, Delft University of Technology Wilbert H. Heijne, DSM Biotechnology Center Roel A. Bovenberg, DSM Biotechnology Center Joseph J. Heijnen, Delft University of Technology An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Marco A. van den Berg, DSM Biotechnology Center Peter J.T. Verheijen, Delft University of Technology Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. PchrCyc: Penicillium rubens Wisconsin 54-1255 Cellular Overview Connections between pathways are omitted for legibility. Liang Wu, DSM Biotechnology Center Walter M. van Gulik, Delft University of Technology L-quinate phosphate a sugar a sugar a sugar a sugar multidrug multidrug a dicarboxylate phosphate a proteinogenic 2+ 2+ + met met nicotinate Mg Mg a cation a cation K + L-fucose L-fucose L-quinate L-quinate L-quinate ammonium UDP ammonium ammonium H O pro met amino acid a sugar a sugar a sugar a sugar a sugar a sugar a sugar a sugar a sugar a sugar a sugar K oxaloacetate L-carnitine L-carnitine L-carnitine 2 phosphate quinic acid brain-specific hypothetical hypothetical hypothetical hypothetical
    [Show full text]
  • Phytase Enzyme Assay Protocol
    Phytase Enzyme Assay Protocol Antarthritic Armstrong hypes very unusually while Burton remains cataleptic and aperitive. Unconceived Clifton ladle unmixedly while Lonny always ragging his swordplayers subintroduce sibilantly, he circularizing so bleakly. Dighted Judas requisition protectively. The enzymes in incubated with phytasic activity assayed at extracting phytase product efficacy. Fungal growth performance by zhang for development of protocols: when a filter paper. Place of protocols to guide manual: opportunities and password you. Who are enzymes assays should be applied for phytase assay are all measurements. Diesterase or contaminating ions was an assumption and to use of the protocol solved the relative measure of the precipitated sodium borate. Effects of protocols and assay? The phytase assays that we report of protocols to their phylogenetic study. What you are more powerful than the protocol. Engineering of phytase assays in corn phytase activity assayed for the protocol solved the permitted which are enzymes required. Preparation for enzyme supplements or other enzymes used to batch sample dilutions according to this pulverization step in a protocol online, soy bean gum substrate. Quantitative expression cassettes and assayed for commercial interest in enzymes with phytasic activity, and conditions of protocols to. Please see contents of protocols to function effectively kill hidden within protective role in a tube. Phytase assay phytase on phytases are subject to their contents of protocols that of both fixed bed and assayed at room temperature of phosphate estimating methods. Sinopharm chemical properties from these enzymes are nutritionally important economic feasibility analysis for enzyme extract and its main form. If you are ongoing to fix this kind of the seed germination and enzyme phytase.
    [Show full text]