Improvement and Culture of Nitrogen Fixing Trees Message from The
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Improvement and Culture of Nitrogen Fixing Trees January 2003 Volume 6 No. 1 Working Party 2.08.02 Web page at: http://iufro.boku.ac.at/iufro/iufronet/d2/wu20802/nl20802.htm Message from the Coordinator Improving Wood and Biomass WP 2.08.02 Production of Some Casuarina Species through Symbiotic Khongsak Pinyopusarerk CSIRO Forestry and Forest Products Association in Egypt PO Box E4008, Kingston, ACT 2604, Australia Tel: + 61 2 6281 8247 Samira R. Mansour Fax: + 61 2 6281 8266 Botany Department Email: [email protected] Faculty of Science Suez Canal University A question often asked is why use nitrogen-fixing trees Ismailia Egypt (NFT) and what is their advantage over non-NFT Tel: +20 64 341418 species? There are many good reasons of course. Since Fax: +20 64 327896 no plant grows without nitrogen and large areas of Email: [email protected] tropical soils have low supplies of this nutrient, NFT Introduction species can establish themselves under these Biological nitrogen fixation has received increasing interest in conditions through their ability to fix nitrogen through recent years for its significance to the ecosystem. Actinorhizal symbiotic micro-organisms that live in root nodules and plants are trees which can input a huge amount of nitrogen to convert atmospheric nitrogen into a usable form. Put in the ecosystem through symbiotic nitrogen (N2) fixation simple words, NFT species do not depend solely on process with filamentous soil bacteria (Frankia). The N2 which is fixed by actinorhizal plants is comparable to the that available nitrogen in the soils like non-NFT species. The fixed by legumes. Casuarina species are the most important majority of more than 15,000 species in the family actinorhizal plants in the tropics and sub-tropics. Leguminosae and in other families such as Casuarinaceae and Elaegnaceae are known to fix nitrogen. It is therefore not uncommon for NFT species to find their way into all forms of planting for a wide variety of products and services. Members will notice a new IUFRO logo on this cover page. The IUFRO board agreed to adopt this new logo as part of its strategy that aims at showing IUFRO as a modern organisation. This followed a word change ‘forestry’ to ‘forest’ in the name of this organisation. I take this opportunity to welcome many new members of this IUFRO Working Party. Please feel free to use this newsletter as a venue for sharing information and your experiences on NFT species with other members and public audiences. Three-year-old Casuarina cunninghamiana x C. glauca hybrid in a Frankia inoculation trial at Suez Canal University Farm, Egypt Improvement and Culture of Nitrogen Fixing Trees Casuarina species are widely grown in Egypt for The results demonstrate the need for a suitable multipurpose uses. The major benefits of casuarinas in Egypt Frankia - Casuarina combination to ensure maximum yield are the control of shifting sand, windbreaks for villages and and high nitrogen production in Casuarina plantations. It is agricultural sites from strong coastal winds, and for the concluded that inoculation has significant effect in improving production of wood for construction and firewood. Today, Casuarina plantations and these results confirm the findings casuarinas are used as a pioneer species for soil reclamation. of Reddell (1990) and Sougafoura et al. (1989). With agriculture expansion, more land needs to be reclaimed to meet increasing food demand and thus Casuarina trees are Acknowledgements in high demand for use on-farms. Improving Casuarina The study was a part of project funded by the Ministry of species for wood and biomass production as well as high Agriculture of Egypt in cooperation with Biotechnologie des capability for nitrogen fixation can be achieved by inoculation Symbioses Forestières Tropicales (ORSTOM/CIRAD-Forêt, (Reddell 1990; Mansour et al. 1996). Therefore, selection for France). The experimental design was established in the best Frankia - Casuarina combinations is essential. consultation with Dr Dwight Baker, Panlabs (USA). The present study reports findings of a study carried out References at Suez Canal University Farm, Egypt for the selection Mansour, S.R, Zaied, A. and Dewedar A. 1996. Performance of two of suitable Frankia for C. cunninghamiana and Casuarina species inoculated with pure culture of Frankia C. cunninghamiana x C. glauca hybrid. under field conditions. Egypt Journal of Microbiology 31 (2):287-302. Materials and Methods The experiment was established in new cultivated desert site Reddell, P. 1990. Increasing productivity in planting of Casuarina by at Suez Canal University Farm (latitude 30°40′N, longitude inoculation with Frankia. In: El-Lakany, M.H., Turnbull, 32°15′E, altitude 50m asl). The mean annual rainfall is less J.W. and Brewbaker, J.L. (eds.) Advances in Casuarina than 20 mm and the mean annual temperature ranges from Research and Utilization. Proceedings of the second 18° to 35°C. The trial was irrigated twice a week in the first international casuarina workshop, Desert Development year and once a week in subsequent years. Each plant Center, American University, Cairo, Egypt. p. 133-140. received about 250 mL of water per each irrigation time. Sougoufara, B., Diem, H.G. and Dommergues, Y.R. 1989. Response Soil characteristics at the site are typically sandy with pH 8.1 of field-grown Casuarina equisetifolia to inoculation with and low N content (total N 0.005%), P (available P 3.4 ppm) Frankia strain ORS021002 entrapped in alginate beads. and organic carbon (0.12%). Casuarina cunninghamiana and Plant and Soil 118: 133-137. C. cunninghamiana x C. glauca hybrid were chosen for the experiment. Inoculation was carried by using pure Frankia culture Cg14 and crushed nodule suspension. Pure culture was propagated for three weeks and then harvested hyphae were Performance of Two Exotic homogenized. Each plant received 0.01PCV (Packed Cell Volume) (50 µg protein mL-1). Crushed nodule suspension Nitrogen-Fixing Tree Species at materials were collected, surface sterilized, homogenized and suspended in sterilized distilled water and each plant received Chittagong University Campus, 0.01 gm mL-1 nodule homogenate. Un-inoculated plants Bangladesh served as a control. The experiment was a randomized complete block design with four replicates, 80 plants/plot and 2.5 m x 2.5 m spacing. Plant height, wood production, plant M. K. Hossain and B. M. Khan dry weights and total nitrogen content were assessed three Institute of Forestry and Environmental Sciences years after planting out. Chittagong University Chittagong-4331, Bangladesh Results and Discussion E-mail: [email protected] Inoculated plants with either pure culture or crushed nodule suspension were more productive compared to un-inoculated Introduction plants. Pure culture of Frankia strain Cg14 produced greater Gliricidia sepium (Jacq.) Walp. is believed to be the most values for plant height, wood production, biomass and total widely cultivated exotic tree in Bangladesh. It grows in a wide nitrogen content at age 3 years than crushed nodules variety of soils and climates up to 1000 m asl (Hughes 1987). (Table 1). The Casuarina hybrid appears to perform better It is used in many parts of the tropics for fuel wood, living than pure C. cunninghamiana in all traits. fences, fodder, soil improvement and plantation shade species. The leaves by their chemical composition have a high Table 1. Effect of inoculation and inoculation types on plant height, potential for use as animal feeds and have better nutrient wood and biomass production and nitrogen yield of two Casuarina digestive values than grasses. The ability of Gliricidia to species at three years after planting produce a wide range of products including poles, green manure and bee forage and its management under a variety of Estimated Species Inoculum Plant wood Plant dry Nitrogen silvicultural regimes both in the pure woodlots and the Height production Weight yield agroforestry mixtures have some advantages over other (m) (m3ha-1)(kg tree-1) (kg ha-1) species (Falvey 1982). Casuarina hybrid Control 2.1 0.8 1.5 27.0 Pure culture 6.1 27.5 27.5 481.6 Calliandra calothyrsus Meissn. is a small (2-12m tall) Crushed nodule 4.4 14.6 17.7 311.5 leguminous tree with a trunk diameter of up to 30 cm. Tree develops a dense canopy and has white to red brown bark. C. cunninghamiana Control 1.8 0.4 0.9 10.9 The plant is native to the humid and subhumid regions of Pure culture 5.1 18.2 18.9 336.2 Crushed nodule 4.2 16.1 15.2 278.9 Central America and Mexico and is found from southern Mexico to central Panama, approximately between 8° and 2 Improvement and Culture of Nitrogen Fixing Trees 19°N (MacQueen 1992). The plant is not very drought Both G. sepium and C. calothyrsus are extremely versatile tolerant although it is able to withstand some dry periods. In plants being used in a number of agroforestry applications humid climates it is evergreen, whereas in areas with a long throughout the tropics. They produce high quality fuel wood, dry season it is semi-deciduous. living fences, green manure, shade and forage. They grow over a wide range of soil types and climates and are often The objective of the present study was to assess individual outstanding on infertile sites where they are extensively used and comparative growth performance of these two valuable for land reclamation. The high yielding ability and high nitrogen-fixing exotic tree species in the Chittagong protein content of C. calothyrsus indicates the potential forage University Campus area, Bangladesh. value in the tropics. From the present findings it may be concluded that C. calothyrsus may be used in further Materials and Methods plantation programs in Bangladesh with different silvicultural A small amount of C.