DOCSLIB.ORG

Comparative Analysis of Courtship in <I>Agelenopsis</I> Funnel-Web Spiders

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Comparative Analysis of Courtship in <I>Agelenopsis</I> Funnel-Web Spiders University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 5-2012 Comparative analysis of courtship in Agelenopsis funnel-web spiders (Araneae, Agelenidae) with an emphasis on potential isolating mechanisms Audra Blair Galasso [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Behavior and Ethology Commons Recommended Citation Galasso, Audra Blair, "Comparative analysis of courtship in Agelenopsis funnel-web spiders (Araneae, Agelenidae) with an emphasis on potential isolating mechanisms. " PhD diss., University of Tennessee, 2012. https://trace.tennessee.edu/utk_graddiss/1377 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Audra Blair Galasso entitled "Comparative analysis of courtship in Agelenopsis funnel-web spiders (Araneae, Agelenidae) with an emphasis on potential isolating mechanisms." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, with a major in Ecology and Evolutionary Biology. Susan E. Riechert, Major Professor We have read this dissertation and recommend its acceptance: Gordon M. Burghardt, James A. Fordyce, Todd M. Freeberg Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Comparative analysis of courtship in Agelenopsis funnel-web spiders (Araneae, Agelenidae) with an emphasis on potential isolating mechanisms A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Audra Blair Galasso May 2012 Copyright © 2012 by Audra Blair Galasso All rights reserved. ii DEDICATION I dedicate this dissertation to my parents, Joyce and Everett Payne, who always encouraged in me a love of books, knowledge and the natural world. iii ACKNOWLEDGEMENTS I wish to thank my advisor, Susan Riechert, for providing me with intellectual freedom and steadfast support throughout my time in graduate school. She introduced me to the fascinating world of spider behavior, and her lab was always an interesting place to be. I would also like to thank my committee members, Gordon Burghardt, Jim Fordyce and Todd Freeberg. Our interactions in and out of the classroom broadened my understanding of animal behavior and evolutionary biology. I would also like to express my appreciation to the Department of Ecology & Evolutionary Biology, which provided me with a teaching assistantship and an intellectually stimulating environment. I would like to acknowledge those individuals who helped me collect spiders for this project: Steve Galasso, Kim Kennard, Julie Morgan, Jonathan Pruitt and Casey Willingham. I am grateful to George Uetz for allowing me to use his vibration recording equipment at the University of Cincinnati and to his students, Shira Gordon and Brian Moskalik for assistance with that equipment. My fellow students offered stimulating discussion and support, particularly Mackenzie Taylor and my fellow Riechert Lab members – Jonathan Pruitt, Sarah Duncan, Jamie Troupe, Sara Bengston and Austin Dillon. I would also like to acknowledge funding for this project, which came from NSF grant 0315901 from the Evolutionary Processes Program to Susan Riechert, an EEB Summer Research Grant and the Vincent Roth Fund for Systematic Research from the American Arachnological Society. iv Finally, I wish to express deep appreciation to my arachnophobic husband, Steve Galasso, who moved to Knoxville to support my graduate education and even helped me collect and feed spiders. v ABSTRACT The genus Agelenopsis (Araneae, Agelenidae) is a group of morphologically similar funnel-web spiders with overlapping habitat requirements and geographic ranges. Yet, molecular evidence suggests this group has undergone recent speciation with no evidence of hybridization. In this dissertation, I explored courtship divergence as a possible explanation for the formation and coexistence of these species. Courtship behavior patterns, sequences, and vibratory signals were compared across 12 Agelenopsis species and three related outgroup species. Courtship in Agelenopsis was found to be comparatively long but not completely species-specific. To investigate mechanisms of reproductive isolation within the genus, interspecific crosses were staged in the lab between species for two closely related species pairs, one pair being sympatric in distribution and the other peripatric. Vibratory courtship did not function as a reproductive isolating mechanism for either pair. In the sympatric species pair, males displayed significantly less courtship toward heterospecific females than to conspecific females and no interspecific copulations were attempted. Females did not reject males based on their vibratory courtship, rather males rejected females, apparently based on chemical cues. In contrast, there was interspecific courtship and copulation in the peripatric species pair. However, males appeared to encounter mechanical difficulties copulating with heterospecific females and no hybrid offspring were produced. Thus, the sympatric pair was reproductively isolated by barriers acting early in the mating vi process, while the geographically isolated pair appeared to be isolated by barriers acting very late in the mating process. Though these results did not support a role for vibratory courtship in reproductive isolation, vibratory courtship appeared to be quite important in Agelenopsis. Males continued to court females even after females accepted them, and even behavior occurring late in the courtship sequence varied among species. In comparison to the two more distantly related outgroup species, vibratory courtship in Agelenopsis seems to be under relatively strong sexual selection for long, complex displays. vii TABLE OF CONTENTS INTRODUCTION .................................................................................................. 1 Courtship Function and Evolution ..................................................................... 2 The genus Agelenopsis: Natural History and Speciation ................................... 3 Goals of the Dissertation Research ................................................................... 5 Literature Cited .................................................................................................. 7 CHAPTER I COMPARATIVE ANALYSIS OF COURTSHIP IN FUNNEL-WEB SPIDERS .............................................................................................................. 9 Abstract ........................................................................................................... 10 Introduction ..................................................................................................... 11 Methods .......................................................................................................... 17 Collections and rearing conditions ............................................................... 17 Courtship trials ............................................................................................. 19 Quantitative description of courtship ............................................................ 20 Relative durations of two courtship phases and incidence of mixed phase behavior ....................................................................................................... 23 Tests to determine if species can be discriminated by courtship behavior .. 23 Phylogenetic analysis of courtship ............................................................... 26 Results ............................................................................................................ 28 General courtship features ........................................................................... 28 viii Behavior patterns exhibited ......................................................................... 30 Test for differences in rearing history ........................................................... 31 Species differences in courtship variables ................................................... 32 Assessing prevalence of courtship after female acceptance ....................... 33 Species-specificity of courtship .................................................................... 34 Phylogenetic analyses ................................................................................. 36 Discussion ....................................................................................................... 39 General features of funnel-web spider courtship ......................................... 39 Possible mechanisms of courtship divergence in Agelenopsis: Drift and phylogeny .................................................................................................... 43 Possible mechanisms of courtship divergence in Agelenopsis: Reinforcement ............................................................................................. 44 Possible mechanisms of courtship divergence in Agelenopsis: Sexual selection ......................................................................................................
Load more

Recommended publications
  • Untangling Taxonomy: a DNA Barcode Reference Library for Canadian Spiders
    Molecular Ecology Resources (2016) 16, 325–341 doi: 10.1111/1755-0998.12444 Untangling taxonomy: a DNA barcode reference library for Canadian spiders GERGIN A. BLAGOEV, JEREMY R. DEWAARD, SUJEEVAN RATNASINGHAM, STEPHANIE L. DEWAARD, LIUQIONG LU, JAMES ROBERTSON, ANGELA C. TELFER and PAUL D. N. HEBERT Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada Abstract Approximately 1460 species of spiders have been reported from Canada, 3% of the global fauna. This study provides a DNA barcode reference library for 1018 of these species based upon the analysis of more than 30 000 specimens. The sequence results show a clear barcode gap in most cases with a mean intraspecific divergence of 0.78% vs. a min- imum nearest-neighbour (NN) distance averaging 7.85%. The sequences were assigned to 1359 Barcode index num- bers (BINs) with 1344 of these BINs composed of specimens belonging to a single currently recognized species. There was a perfect correspondence between BIN membership and a known species in 795 cases, while another 197 species were assigned to two or more BINs (556 in total). A few other species (26) were involved in BIN merges or in a combination of merges and splits. There was only a weak relationship between the number of specimens analysed for a species and its BIN count. However, three species were clear outliers with their specimens being placed in 11– 22 BINs. Although all BIN splits need further study to clarify the taxonomic status of the entities involved, DNA bar- codes discriminated 98% of the 1018 species. The present survey conservatively revealed 16 species new to science, 52 species new to Canada and major range extensions for 426 species.
    [Show full text]
  • Spider Records from Colin-Cornwall Lakes Wildland Provincial Park
    SPIDER RECORDS FROM COLIN-CORNWALL LAKES WILDLAND PROVINCIAL PARK Larinioides cornutus Thanatus coloradensis (Photo by D. Buckle) (Photo by D. Buckle) Prepared by Wayne Nordstrom1 and Don Buckle2 March 2004 1Alberta Natural Heritage Information Centre Parks and Protected Areas Division Alberta Community Development 2 620 Albert Avenue Saskatoon, SK S7N 1G7 Table of Contents 1. Introduction.................................................................................................................................. 1 2. The Study Site.............................................................................................................................. 1 3. Methods ....................................................................................................................................... 3 3.1 Survey Dates....................................................................................................................... 3 3.2 Collection of Spiders .......................................................................................................... 3 3.3 Identification of Spiders ..................................................................................................... 4 4. Results and Discussion ................................................................................................................ 4 5. Conclusion ...................................................................................................................................11 6. Acknowlegements........................................................................................................................11
    [Show full text]
  • Sexual Selection Research on Spiders: Progress and Biases
    Biol. Rev. (2005), 80, pp. 363–385. f Cambridge Philosophical Society 363 doi:10.1017/S1464793104006700 Printed in the United Kingdom Sexual selection research on spiders: progress and biases Bernhard A. Huber* Zoological Research Institute and Museum Alexander Koenig, Adenauerallee 160, 53113 Bonn, Germany (Received 7 June 2004; revised 25 November 2004; accepted 29 November 2004) ABSTRACT The renaissance of interest in sexual selection during the last decades has fuelled an extraordinary increase of scientific papers on the subject in spiders. Research has focused both on the process of sexual selection itself, for example on the signals and various modalities involved, and on the patterns, that is the outcome of mate choice and competition depending on certain parameters. Sexual selection has most clearly been demonstrated in cases involving visual and acoustical signals but most spiders are myopic and mute, relying rather on vibrations, chemical and tactile stimuli. This review argues that research has been biased towards modalities that are relatively easily accessible to the human observer. Circumstantial and comparative evidence indicates that sexual selection working via substrate-borne vibrations and tactile as well as chemical stimuli may be common and widespread in spiders. Pattern-oriented research has focused on several phenomena for which spiders offer excellent model objects, like sexual size dimorphism, nuptial feeding, sexual cannibalism, and sperm competition. The accumulating evidence argues for a highly complex set of explanations for seemingly uniform patterns like size dimorphism and sexual cannibalism. Sexual selection appears involved as well as natural selection and mechanisms that are adaptive in other contexts only. Sperm competition has resulted in a plethora of morpho- logical and behavioural adaptations, and simplistic models like those linking reproductive morphology with behaviour and sperm priority patterns in a straightforward way are being replaced by complex models involving an array of parameters.
    [Show full text]
  • SPIDERS of WASHINGTON COUNTY, MISSISSIPPI Orrey P. Young Southern Field Crop Insect Management Laboratory USDA-ARS, P.O. Box
    Young, O . P., T. C . Lockley and G . B . Edwards . 1989 . Spiders of Washington County, Mississippi . J . Arachnol ., 17 :27-41 . SPIDERS OF WASHINGTON COUNTY, MISSISSIPPI Orrey P. Young Southern Field Crop Insect Management Laboratory USDA-ARS, P.O. Box 346 Stoneville, Mississippi 38776 USA Timothy C. Lockley Imported Fire Ant Station USDA-APHIS-PPQ 3505 25th Avenue Gulfport, Mississippi 39501 USA and G. B. Edwards Florida State Collection of Arthropods Division of Plant Industry Florida Dept. Agric. & Cons . Serv. P.O. Box 1269 Gainesville, Florida 32602 USA ABSTRACT Over a seven-year period, approximately 35,000 spiders representing 26 families, 133 genera, and 234 species were captured in Washington County, Mississippi, by pitfall, sweepnet, vacuum, bag, and hand. Specimens were collected in 10 different habitat types and in four vegetational strata . Old-field habitats yielded the most species (152) and residential lawns the fewest (14) . Considering all habitats sampled, the ground layer produced 111 species, the herbaceous strata 133, the shrub layer 49, and the tree strata 30 species . The sweepnet method of capture obtained 128 species, pitfall 95, hand 61, vacuum 53, and bagging 19 species. The largest number of species were obtained in spring and early summer (maximum of 125 in May), with the fewest in mid-winter (Jan . = 24) . Twenty-one species were considered abundant, 51 common, 67 uncommon, and 95 rare . Additions to the state list of Dorris (1972) number 102 species, for a new state total of 364 species . A comparison with the North American fauna and with other surveys indicates that Washington County is underrepresented both in cursorial forms active on the soil surface and web-spinning forms typical of undisturbed habitats .
    [Show full text]
  • Three New Coelotes Spiders (Araneae
    Zoological Studies 40(2): 127-133 (2001) Three New Coelotes Spiders (Araneae: Amaurobiidae) from Taiwan Xinping Wang1,*, I-Min Tso2 and Hai-Yin Wu3 1Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th Street, NY 10024, USA 2Department of Biology, Tunghai University, Taichung, Taiwan 407, R.O.C. 3Institute of Natural Resource Management, Tunghwa University, Hwalian, Taiwan 974, R.O.C. (Accepted January 4, 2001) Xinping Wang, I-Min Tso and Hai-Yin Wu (2001) Three new Coelotes spiders (Araneae: Amaurobiidae) from Taiwan. Zoological Studies 40(2): 127-133. Five species of the spider genus Coelotes were collected from pitfall traps in the Hui-Sun Experimental Forest Station in the central mountains of Taiwan. These include Coelotes xinhuiensis Chen, 1984, Paracoelotes taiwanensis Wang and Ono, 1998; and 3 new species: Coelotes bifida sp. n., C. latus sp. n., and C. longus sp. n. The new species are described and illustrated, and the spinneret morphology and natural history of the new species C. bifida and C. latus are reported. The current number of coelotine spider species in Taiwan is increased to 12. The species, Wadotes primus Fox, 1937, which was described from Hong Kong, is newly transferred to the genus Coelotes (new combination). Key words: Coelotes bifida, Coelotes latus, Coelotes longus, Spinnerets, Hui-Sun Forest Area. Coelotes spiders are widespread and spe- wanese arachnofaunal surveys, many unrecorded cious in East Asia (Yaginuma 1986, Wang et al. species were found in these collections. Among the 1990, Wang and Ono 1998), with currently more than specimens obtained, coelotine spiders were quite 100 described species.
    [Show full text]
  • Arachnida, Solifugae) with Special Focus on Functional Analyses and Phylogenetic Interpretations
    HISTOLOGY AND ULTRASTRUCTURE OF SOLIFUGES Comparative studies of organ systems of solifuges (Arachnida, Solifugae) with special focus on functional analyses and phylogenetic interpretations HISTOLOGIE UND ULTRASTRUKTUR DER SOLIFUGEN Vergleichende Studien an Organsystemen der Solifugen (Arachnida, Solifugae) mit Schwerpunkt auf funktionellen Analysen und phylogenetischen Interpretationen I N A U G U R A L D I S S E R T A T I O N zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Anja Elisabeth Klann geboren am 28.November 1976 in Bremen Greifswald, den 04.06.2009 Dekan ........................................................................................................Prof. Dr. Klaus Fesser Prof. Dr. Dr. h.c. Gerd Alberti Erster Gutachter .......................................................................................... Zweiter Gutachter ........................................................................................Prof. Dr. Romano Dallai Tag der Promotion ........................................................................................15.09.2009 Content Summary ..........................................................................................1 Zusammenfassung ..........................................................................5 Acknowledgments ..........................................................................9 1. Introduction ............................................................................
    [Show full text]
  • Arachnida: Araneae) from the Middle Eocene Messel Maar, Germany
    Palaeoentomology 002 (6): 596–601 ISSN 2624-2826 (print edition) https://www.mapress.com/j/pe/ Short PALAEOENTOMOLOGY Copyright © 2019 Magnolia Press Communication ISSN 2624-2834 (online edition) PE https://doi.org/10.11646/palaeoentomology.2.6.10 http://zoobank.org/urn:lsid:zoobank.org:pub:E7F92F14-A680-4D30-8CF5-2B27C5AED0AB A new spider (Arachnida: Araneae) from the Middle Eocene Messel Maar, Germany PAUL A. SELDEN1, 2, * & torsten wappler3 1Department of Geology, University of Kansas, 1475 Jayhawk Boulevard, Lawrence, Kansas 66045, USA. 2Natural History Museum, Cromwell Road, London SW7 5BD, UK. 3Hessisches Landesmuseum Darmstadt, Friedensplatz 1, 64283 Darmstadt, Germany. *Corresponding author. E-mail: [email protected] The Fossil-Lagerstätte of Grube Messel, Germany, has Thomisidae and Salticidae (Schawaller & Ono, 1979; produced some of the most spectacular fossils of the Wunderlich, 1986). The Pliocene lake of Willershausen, Paleogene (Schaal & Ziegler, 1992; Gruber & Micklich, produced by solution of evaporites and subsequent collapse, 2007; Selden & Nudds, 2012; Schaal et al., 2018). However, has produced some remarkably preserved arthropod fossils few arachnids have been discovered or described from this (Briggs et al., 1998), including numerous spider families: World Heritage Site. An araneid spider was reported by Dysderidae, Lycosidae, Thomisidae and Salticidae (Straus, Wunderlich (1986). Wedmann (2018) reported that 160 1967; Schawaller, 1982). All of these localities are much spider specimens were known from Messel although, sadly, younger than Messel. few are well preserved. She figured the araneid mentioned by Wunderlich (1986) and a nicely preserved hersiliid (Wedmann, 2018: figs 7.8–7.9, respectively). Wedmann Material and methods (2018) mentioned six opilionids yet to be described, and figured one (Wedmann, 2018: fig.
    [Show full text]
  • Gelenopsis Naevia Walckenaer, 1842 (Grass Spider)Venom
    STUDIES ON ANTIMICROBIAL AND HAEMOLYTIC ACTIVITIES, PROTEIN PROFILE AND TRANSCRIPTOMES OF AGELENOPSIS NAEVIA WALCKENAER, 1842 (GRASS SPIDER)VENOM BY JAMILA AHMED DEPARTMENT OF BIOLOGICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA AUGUST, 2016 i STUDIES ON ANTIMICROBIAL AND HAEMOLYTIC ACTIVITIES, PROTEIN PROFILE AND TRANSCRIPTOMES OF AGELENOPSIS NAEVIA WALCKENAER, 1842 (GRASS SPIDER)VENOM BY JamilaAHMED M. Sc/Sci/32878/2012-2013 A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA. IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN BIOLOGY DEPARTMENT OF BIOLOGICAL SCIENCES, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA AUGUST, 2016 ii DECLARATION I declare that the work in this dissertation, entitled, ―Studies on antimicrobial and haemolytic activities, protein profile and transcriptomes of Agelenopsis naevia Walckenaer, 1842 (grass spider) venom” was carried out by me in the Department of Biological Sciences, Faculty of Science, Ahmadu Bello University Zaria under the supervision of Prof. I. S. Ndams and Dr. D. M. Shehu. All information derived from the literature has been duly acknowledged in the text and a list of references provided. No part of this dissertation was previously presented for another degree or diploma at any university. Jamila Ahmed ----------------------------------- -------------------------------- Signature Date iii CERTIFICATION This dissertation, entitled STUDIES ON ANTIMICROBIAL AND HAEMOLYTIC ACTIVITIES, PROTEIN PROFILE AND TRANSCRIPTOMES OF AGELENOPSIS NAEVIA WALCKENAER, 1842 (GRASS SPIDER) VENOMby Jamila Ahmed meets the regulation governing the award of Master of Science in Biology of the Ahmadu Bello University, Zaria, and is approved for its contribution to knowledge and literary presentation. Prof. I. S. Ndams------------------------- -------------------------- Chairman Supervisory CommitteeSignature Date Department of Biological Sciences, Faculty of Science, Ahmadu Bello University, Zaria Dr.
    [Show full text]
  • Arthropods of Elm Fork Preserve
    Arthropods of Elm Fork Preserve Arthropods are characterized by having jointed limbs and exoskeletons. They include a diverse assortment of creatures: Insects, spiders, crustaceans (crayfish, crabs, pill bugs), centipedes and millipedes among others. Column Headings Scientific Name: The phenomenal diversity of arthropods, creates numerous difficulties in the determination of species. Positive identification is often achieved only by specialists using obscure monographs to ‘key out’ a species by examining microscopic differences in anatomy. For our purposes in this survey of the fauna, classification at a lower level of resolution still yields valuable information. For instance, knowing that ant lions belong to the Family, Myrmeleontidae, allows us to quickly look them up on the Internet and be confident we are not being fooled by a common name that may also apply to some other, unrelated something. With the Family name firmly in hand, we may explore the natural history of ant lions without needing to know exactly which species we are viewing. In some instances identification is only readily available at an even higher ranking such as Class. Millipedes are in the Class Diplopoda. There are many Orders (O) of millipedes and they are not easily differentiated so this entry is best left at the rank of Class. A great deal of taxonomic reorganization has been occurring lately with advances in DNA analysis pointing out underlying connections and differences that were previously unrealized. For this reason, all other rankings aside from Family, Genus and Species have been omitted from the interior of the tables since many of these ranks are in a state of flux.
    [Show full text]
  • The Generic and Specific Status of Four Ohio Spiders of the Genus Agelenopsis
    THE OHIO JOURNAL OF SCIENCE VOL. XLI MARCH, 1941 No. 2 THE GENERIC AND SPECIFIC STATUS OF FOUR OHIO SPIDERS OF THE GENUS AGELENOPSIS PAUL J. SEYLER, Ohio State University INTRODUCTION Every taxonomist sooner or later faces a situation in which he must determine whether he is dealing with a single highly variable species, or with several species which appears to intergrade. This problem confronting us has been recognized by Petrunkevitch, Emerton, Comstock, Chamberlin, Ivie, Gertsch and others. Arachnologists have been perplexed by the variability of a species which has been considered Agelena naevia Walckenaer as the following quotations will indicate. Petrunkevitch (1925) on page 561 states: "It does not require prolonged study to show that variation in size in A gelena naevia is very great. Specimens collected in the same locality, often under the same piece of loose bark, guarding their cocoons with eggs, and therefore in the same stage of maturity, are occasionally twice the size of others. The fact that two such distinguished arachnologists as Graf Keyserling and Becker have described two new species of Agelena from North America, which long since have been recognized as synonyms of A. naevia, shows that the variation in structure is also sufficient to be considered as specific in absence of intergradient forms." Emerton (1890) recognized the great variation that exists in both the palpus of the male and epigynum of the female. He has illustrated four types of palps and seven variations in the atrial opening of the epigynum. He says that, "The shape of the external opening of the epigynum is even more variable than that of the palpal organ." Comstock (1912), page 586, in his spider book: "This species is either a variable one in the form of the palpi of the males and 51 52 PAUL J.
    [Show full text]
  • Caracterização Proteometabolômica Dos Componentes Da Teia Da Aranha Nephila Clavipes Utilizados Na Estratégia De Captura De Presas
    UNIVERSIDADE ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO” INSTITUTO DE BIOCIÊNCIAS – RIO CLARO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS BIOLOGIA CELULAR E MOLECULAR Caracterização proteometabolômica dos componentes da teia da aranha Nephila clavipes utilizados na estratégia de captura de presas Franciele Grego Esteves Dissertação apresentada ao Instituto de Biociências do Câmpus de Rio . Claro, Universidade Estadual Paulista, como parte dos requisitos para obtenção do título de Mestre em Biologia Celular e Molecular. Rio Claro São Paulo - Brasil Março/2017 FRANCIELE GREGO ESTEVES CARACTERIZAÇÃO PROTEOMETABOLÔMICA DOS COMPONENTES DA TEIA DA ARANHA Nephila clavipes UTILIZADOS NA ESTRATÉGIA DE CAPTURA DE PRESA Orientador: Prof. Dr. Mario Sergio Palma Co-Orientador: Dr. José Roberto Aparecido dos Santos-Pinto Dissertação apresentada ao Instituto de Biociências da Universidade Estadual Paulista “Júlio de Mesquita Filho” - Campus de Rio Claro-SP, como parte dos requisitos para obtenção do título de Mestre em Biologia Celular e Molecular. Rio Claro 2017 595.44 Esteves, Franciele Grego E79c Caracterização proteometabolômica dos componentes da teia da aranha Nephila clavipes utilizados na estratégia de captura de presas / Franciele Grego Esteves. - Rio Claro, 2017 221 f. : il., figs., gráfs., tabs., fots. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro Orientador: Mario Sergio Palma Coorientador: José Roberto Aparecido dos Santos-Pinto 1. Aracnídeo. 2. Seda de aranha. 3. Glândulas de seda. 4. Toxinas. 5. Abordagem proteômica shotgun. 6. Abordagem metabolômica. I. Título. Ficha Catalográfica elaborada pela STATI - Biblioteca da UNESP Campus de Rio Claro/SP Dedico esse trabalho à minha família e aos meus amigos. Agradecimentos AGRADECIMENTOS Agradeço a Deus primeiramente por me fortalecer no dia a dia, por me capacitar a enfrentar os obstáculos e momentos difíceis da vida.
    [Show full text]
  • Common Kansas Spiders
    A Pocket Guide to Common Kansas Spiders By Hank Guarisco Photos by Hank Guarisco Funded by Westar Energy Green Team, American Arachnological Society and the Chickadee Checkoff Published by the Friends of the Great Plains Nature Center i Table of Contents Introduction • 2 Arachnophobia • 3 Spider Anatomy • 4 House Spiders • 5 Hunting Spiders • 5 Venomous Spiders • 6-7 Spider Webs • 8-9 Other Arachnids • 9-12 Species accounts • 13 Texas Brown Tarantula • 14 Brown Recluse • 15 Northern Black Widow • 16 Southern & Western Black Widows • 17-18 Woodlouse Spider • 19 Truncated Cellar Spider • 20 Elongated Cellar Spider • 21 Common Cellar Spider • 22 Checkered Cobweb Weaver • 23 Quasi-social Cobweb Spider • 24 Carolina Wolf Spider • 25 Striped Wolf Spider • 26 Dotted Wolf Spider • 27 Western Lance Spider • 28 Common Nurseryweb Spider • 29 Tufted Nurseryweb Spider • 30 Giant Fishing Spider • 31 Six-spotted Fishing Spider • 32 Garden Ghost Spider Cover Photo: Cherokee Star-bellied Orbweaver ii Eastern Funnelweb Spider • 33 Eastern and Western Parson Spiders • 34 Garden Ghost Spider • 35 Bark Crab Spider • 36 Prairie Crab Spider • 37 Texas Crab Spider • 38 Black-banded Crab Spider • 39 Ridge-faced Flower Spider • 40 Striped Lynx Spider • 41 Black-banded Common and Convict Zebra Spiders • 42 Crab Spider Dimorphic Jumping Spider • 43 Bold Jumping Spider • 44 Apache Jumping Spider • 45 Prairie Jumping Spider • 46 Emerald Jumping Spider • 47 Bark Jumping Spider • 48 Puritan Pirate Spider • 49 Eastern and Four-lined Pirate Spiders • 50 Orchard Spider • 51 Castleback Orbweaver • 52 Triangulate Orbweaver • 53 Common & Cherokee Star-bellied Orbweavers • 54 Black & Yellow Garden Spider • 55 Banded Garden Spider • 56 Marbled Orbweaver • 57 Eastern Arboreal Orbweaver • 58 Western Arboreal Orbweaver • 59 Furrow Orbweaver • 60 Eastern Labyrinth Orbweaver • 61 Giant Long-jawed Orbweaver • 62 Silver Long-jawed Orbweaver • 63 Bowl and Doily Spider • 64 Filmy Dome Spider • 66 References • 67 Pocket Guides • 68-69 1 Introduction This is a guide to the most common spiders found in Kansas.
    [Show full text]