Synergistic Combination Chemotherapy of Camptothecin and Floxuridine through Self assembly of Amphiphilic Drug Drug Conjugate
Minxi Hu, Ping Huang, Yao Wang, Yue Su, Linzhu Zhou, Xinyuan Zhu,* and Deyue Yan*
School of Chemistry and Chemical Engineering, Shanghai Key Lab of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China
* Correspondence to: [email protected]; [email protected]. Fax: +86 21 54741297
Table of Contents
Supplementary Figures
1 Figure S1: H NMR spectra of CPT and CPT COOH in DMSO d6.
Figure S2: FTIR spectra of CPT, FUDR, and CPT FUDR conjugate.
Figure S3: (A) UV/Vis spectra of CPT, FUDR and CPT FUDR conjugate in acetonitrile. (B) Fluorescence emission spectra of CPT (λ ex = 360 nm, λ em = 430 nm) and
CPT FUDR conjugate (λ ex = 360 nm, λ em = 426 nm) in DMSO.
Figure S4: The UV absorbance of DPH in an aqueous solution of CPT FUDR nanoparticles with various concentrations. The CAC value of CPT FUDR nanoparticles is about 15 M.
Figure S5: Influence of storage on (A) diameter (the diameter measured in Day 1 was set as control, and the data are presented as ratios to the control) and (B) PDI of CPT FUDR nanoparticles with the extension of time in water.
Figure S6: (A)Total ion chromatography (TIC) of CPT and CPT FUDR . (B, C) Extracted ion chromatography (EIC) of CPT FUDR (m/z = 693.1835, (M+H +)) and CPT (m/z = 349.1165, (M+H +)). The retention time of CPT FUDR and CPT is 3.92 and 3.79 min, respectively.
Figure S7: (A) Total ion chromatography (TIC) of the cell extracts. (B, C) Extracted ion chromatography (EIC) of CPT FUDR (m/z = 693.1853, (M+H +)) and FUDR (m/z = 349.1205, (M+H +)). The retention time of CPT FUDR and CPT is 3.93, and 3.80 min, respectively.
Figure S8: Flow cytometry histogram profiles of HT 29 cells treated with CPT FUDR nanoparticles containing core encapsulated Nile red for 30 min, 2 h and 4 h.
1 Figure S1. H NMR spectra of CPT and CPT COOH in DMSO d6.
Figure S2. FTIR spectra of CPT, FUDR, and CPT FUDR conjugate.
Figure S3 . (A) UV/Vis spectra of CPT, FUDR and CPT FUDR conjugate in acetonitrile. (B) Fluorescence emission spectra of CPT (λ ex = 360 nm, λ em = 430 nm) and CPT FUDR conjugate (λ ex = 360 nm, λ em = 426 nm) in DMSO.
Figure S4. The UV absorbance of DPH in an aqueous solution of CPT FUDR nanoparticles with various concentrations. The CAC value of CPT FUDR nanoparticles is about 15 M.
Figure S5. Influence of storage on (A) diameter (the diameter measured in Day 1 was set as control, and the data are presented as ratios to the control) and (B) PDI of CPT FUDR nanoparticles with the extension of time in water.
Figure S6. (A)Total ion chromatography (TIC) of CPT and CPT FUDR. (B, C) Extracted ion chromatography (EIC) of CPT FUDR (m/z = 693.1835, (M+H +)) and CPT (m/z = 349.1165, (M+H +)). The retention time of CPT FUDR and CPT is 3.92 and 3.79 min, respectively.
Figure S7. (A) Total ion chromatography (TIC) of the cell extracts. (B, C) Extracted ion chromatography (EIC) of CPT FUDR (m/z = 693.1853, (M+H +)) and FUDR (m/z = 349.1205, (M+H +)). The retention time of CPT FUDR and CPT is 3.93, and 3.80 min, respectively.
Figure S8. Flow cytometry histogram profiles of HT 29 cells treated with CPT FUDR nanoparticles containing core encapsulated Nile red for 30 min, 2 h and 4 h.