DATASHEET Eukaryotic Translation Initiation Factor 4E-Binding

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DATASHEET Eukaryotic Translation Initiation Factor 4E-Binding

DATASHEET Abbexa Ltd, Innovation Centre, Cambridge Science Park, Cambridge, CB4 0EY, UK Telephone: +44 (0) 1223 755950 - Fax: +44 (0) 1223 755951 - E-Mail: [email protected] Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 (4EBP1) Antibody Catalogue No.:abx015717 Western blot analysis using 4E-BP1 antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2). Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 antibody with DAB staining. 4E-BP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa).Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1.Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while (24kDa), the phosphorylated form will not.Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64.Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction. Target: 4EBP1 Reactivity: Human Host: Mouse Clonality: Monoclonal Tested Applications: ELISA, WB, IHC Recommended dilutions: ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. Immunogen: Purified recombinant fragment of 4E-BP1 expressed in E. coli. Purification: Unpurified Ascites. Registered in England: 8475531 - VAT Reg No: GB 167 9240 79 Page 1 DATASHEET Abbexa Ltd, Innovation Centre, Cambridge Science Park, Cambridge, CB4 0EY, UK Telephone: +44 (0) 1223 755950 - Fax: +44 (0) 1223 755951 - E-Mail: [email protected] Isotype: IgG1 Conjugation: Unconjugated Storage: Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. Swiss Prot: Q13541 GeneID: 1978 Gene Symbol: EIF4EBP1 OMIM: 602223 HGNC: 3288 Ensembl: ENSG00000187840 Buffer: Ascitic fluid containing 0.03% sodium azide. Note: This product is for research use only. Registered in England: 8475531 - VAT Reg No: GB 167 9240 79 Page 2.

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Accumulation of Dephosphorylated 4EBP After Mtor Inhibition with Rapamycin Is Sufficient to Disrupt Paracrine Transformation By
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