Complete Genome Sequence of Marinifilaceae Bacterium Strain

Marine Genomics 39 (2018) 1–2

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Marine Genomics

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Complete genome sequence of Mariniﬁlaceae bacterium strain SPP2, isolated from the Antarctic marine sediment

Miho Watanabe a,b,⁎, Hisaya Kojima a, Manabu Fukui a a The Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan b Postdoctoral Research Fellow of the Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-8471, Japan article info abstract

Article history: Mariniﬁlaceae bacterium strain SPP2 is a Gram-negative facultative anaerobe, isolated from the Antarctic marine Received 8 June 2017 sediment. Here, we present the complete genome sequence of Mariniﬁlaceae bacterium strain SPP2, which con- Received in revised form 27 June 2017 sists of 5,718,991 bp with a G + C content of 35.99%. The genome data provides insights of microbial evolution Accepted 27 June 2017 and adaption in the Antarctic marine ecosystem. Available online 1 July 2017 © 2017 Elsevier B.V. All rights reserved. Keywords: Complete genome sequence Antarctica Bacteroidetes

1. Introduction quality scaffolds by using RS_HGAP_Assembly.3 (SMRT Analysis 2.3). The genome sequence was automatically annotated and analyzed Marinifilaceae bacterium strain SPP2 (=NBRC 111151) was isolated through the MiGAP pipeline (Sugawara et al., 2009). In this pipeline, from the marine sediments of Langhovde, Antarctica. Phylogenetic anal- RNAmmer (Lagesen et al., 2007) and tRNAscan-SE (Lowe and Eddy, ysis based on the 16S rRNA gene sequences showed that strain SPP2 was 1997) were used to identify rRNA and tRNA genes, respectively. classified into the family Marinifilaceae, the order Marinilabiliales and MetaGene Annotator (Noguchi et al., 2008) was used for prediction of the class Bacteroidia within the phylum Bacteroidetes (Fig. 1). This iso- open reading frames likely to encode proteins (coding sequences late showed the highest sequence similarities with Marinifilum [CDSs]), and functional annotation was performed based on reference albidiflavum (Xu et al., 2016; 93.8% sequence similarity) and databases, including Reference Sequence (RefSeq), TrEMBL, and Clus- Ancylomarina subtilis (Wu et al., 2016; 94.1%). This strain is a facultative ters of Orthologous Groups (COGs). Manual annotation was performed anaerobic, Gram-negative and weakly motile bacterium. Here, we de- using IMC-GE software (In Silico Biology; Yokohama, Japan). Putative scribe complete genome sequence and annotation of Marinifilaceae bac- CDSs possessing BLASTP matches with N70% coverage, 35% identity terium strain SPP2, which will provide further insight into microbial and E-values b 1×e−5 were considered potentially functional genes. evolution and Antarctic marine ecosystem. The CDSs were annotated as hypothetical proteins when these standard values were not satisfied. Transcription start sites of predicted proteins 2. Data description were corrected based on multiple sequence alignments. The protein- coding genes in the genome were also subjected to analysis on Strain SPP2 was grown aerobically on Marine broth 2216 (Difco) at WebMGA (Wu et al., 2016) for the COGs annotations. Transmembrane 15 °C without shaking. Genomic DNA was extracted from collected helices and signal peptides were predicted by using Phobius (Käll et cells using NucleoSpin Tissue Kit (Macherey-Nagel GmbH & Co., al., 2007). CRISPR loci were distinguished using the CRISPR Recognition Duren, Germany). Library construction and sequencing using the PacBio Tool (Bland et al., 2007). General features of Marinifilaceae bacterium RS II platform were carried out at Takara Bio Inc., Japan. A total of 81,920 strain SPP2 and the MIxS mandatory information were show in Table 1. polymerase reads (1,109,405,149 bp) were generated and filtered. The The final genome was comprised of a single 5,718,991 bp circular resulting sequences (1,108,341,501 bp) were assembled into 1 high chromosome, harboring a total of 4337 predicted protein-coding sequences with a G + C content of 35.99% (Table 2). There were 83 tRNA genes and 22 rRNA genes predicted in the genome. Approximately 70.3% of all coding proteins were assigned to 20 COG functional catego- ⁎ Corresponding author at: The Institute of Low Temperature Science, Hokkaido University, Nishi 8, Kita 19, Kita-ku Sapporo, Hokkaido 060-0819, Japan. ries. The complete genome sequence will provide fundamental informa- E-mail address: [email protected] (M. Watanabe). tion for further research of polar microbiology.

Fig. 1. Phylogenetic tree showing the relationship of Mariniﬁlaceae bacterium strain SPP2 and related representatives. The tree was constructed by the Maximum-Likelihood method with MEGA version 7.0.20 (Kumar et al., 2016) based on ClustalX version 2.1 (Larkin et al., 2007) aligned sequences of 16S rRNA gene. Bootstrap values (percentages of 1000 replications) of ≥50% are shown at nodes.

Nucleotide sequence accession numbers Table 2 General genomic features of Mariniﬁlaceae bacterium strain SPP2.

The complete genome sequence of Marinifilaceae bacterium strain Attributes Value % of total SPP2 (=NBRC 111151) has been deposited at DDBJ/EMBL/GenBank Genome size (bp) 5,718,991 100.00 under the accession number of AP018042. Contig 1 100.00 DNA coding region (bp) 4,836,282 84.57 Acknowledgements DNA G + C content (bp) 2,058,548 35.99 Total genes 4436 100.00 RNA count 105 2.37 This study was supported by a grant-in-aid for Research Fellow of Ja- rRNA 22 0.50 pan Society for the Promotion Science to MW and JSPS KAKENHI Grant tRNA 83 1.87 Number 22370005 to M. Fukui. We also thank members of the 47th Ja- Protein-coding genes 4331 97.63 pan Antarctica Research Expedition (Expedition leader: K. Shiraishi, Genes with function prediction 2349 54.24 Genes assigned to COGs 3044 70.28 NIPR) for logistical assistance. Genes with peptide signals 1186 27.38 Genes with transmembrane helices 907 20.94 CRISPR repeats 3 – Table 1 General features and genome sequencing project information for Marinifilaceae bacterium strain SPP2 according to the MIGS recommendations. References Items Description Bland, C., Ramsey, T.L., Sabree, F., Lowe, M., Brown, K., Kyrpides, N.C., Hugenholtz, P., 2007. General features CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly Classification Domain Bacteria interspaced palindromic repeats. BMC Bioinf. 8, 209. Phylum Bacteroidetes Käll, L., Krogh, A., Sonnhammer, E.L.L., 2007. Advantages of combined transmembrane to- Order Bacteroidales pology and signal peptide prediction: the Phobius web server. Nucleic Acids Res. 35, Family Marinifilaceae W429–W432. Particle shape Rod Kumar, S., Stecher, G., Tamura, K., 2016. MEGA7: molecular evolutionary genetics analysis Gram stain Negative version 7.0 for bigger datasets. Mol. Biol. Evol., msw054 Temperature 0–25 °C Lagesen, K., Hallin, P., Rødland, E.A., Staerfeldt, H.H., Rognes, T., Ussery, D.W., 2007. Salinity 2–4% RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Motility Weakly motile Res. 35, 3100–3108. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., MIxS data Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., et al., 2007. Clustal W and Clustal X ver- Submitted_to_insdc AP018042 (GenBank) sion 2.0. Bioinformatics 23, 2947–2948. Investigation_type Bacteria Lowe, T.M., Eddy, S.R., 1997. tRNAscan-SE: a program for improved detection of transfer Project_name Complete genome sequence of Marinifilaceae bacterium RNA genes in genomic sequence. Nucleic Acids Res. 25, 955–964. fi strain SPP2 Noguchi, H., Taniguchi, T., Itoh, T., 2008. MetaGeneAnnotator: detecting species-speci c Geo_loc_name Langhovde, Antarctica patterns of ribosomal binding site for precise gene prediction in anonymous prokary- otic and phage genomes. DNA Res. 15, 387–396. Collection_date 27-01-2006 Sugawara, H., Ohyama, A., Mori, H., Kurokawa, K., 2009. Microbial GenomeAnnotation Lat_lon Missing Pipeline (MiGAP) for diverse users. Software Demonstrations S001-1-2L. 20th Int. Env_biome Marine sediment (ENVO: 03000033) Conf. Genome Inform. (GIW2009) Poster Software Demonstrations, Yokohama, Env_feature Polar (ENVO: 01000238) Japan. Env_ material Marine mud (ENVO:00005795) Wu, W.J., Zhao, J.X., Chen, G.J., Du, Z.J., 2016. Description of Ancylomarina subtilis gen. nov., Env_package Missing sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and Source:mat_id NBRC 111151 transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Biotic_relationship Free living Marinilabiliales. Int. J. Syst. Evol. Microbiol. 66, 4243–4249. Trophic_level Chemoorganotroph Xu, Z.X., Mu, X., Zhang, H.X., Chen, G.J., Du, Z.J., 2016. Marinifilum albidiflavum sp. nov., iso- Rel_to_oxygen Facultative anaerobic lated from coastal sediment. Int. J. Syst. Evol. Microbiol. 66, 4589–4593. Investigation_type Bacteria_archaea Seq_meth PacBio RS II (Pacific Biosciences) Assembly method RS HGAP v2.3 Finishing_strategy Complete; approximately 194 fold genome coverage Annot_source MiGAP