DOCSLIB.ORG

Rhoh Is a Negative Regulator of Eosinophilopoiesis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Rhoh Is a Negative Regulator of Eosinophilopoiesis Cell Death and Differentiation (2016) 23, 1961–1972 & 2016 Macmillan Publishers Limited, part of Springer Nature. All rights reserved 1350-9047/16 www.nature.com/cdd RhoH is a negative regulator of eosinophilopoiesis Christina Stoeckle1,3, Barbara Geering1,4, Shida Yousefi1,SašaRožman1, Nicola Andina1, Charaf Benarafa2 and Hans-Uwe Simon*,1 Eosinophils are frequently elevated in pathological conditions and can cause tissue damage and disease exacerbation. The number of eosinophils in the blood is largely regulated by factors controlling their production in the bone marrow. While several exogenous factors, such as interleukin-5, have been described to promote eosinophil differentiation, comparatively little is known about eosinophil-intrinsic factors that control their de novo generation. Here, we report that the small atypical GTPase RhoH is induced during human eosinophil differentiation, highly expressed in mature blood eosinophils and further upregulated in patients suffering from a hypereosinophilic syndrome. Overexpression of RhoH increases, in a Rho-associated protein kinase- dependent manner, the expression of GATA-2, a transcription factor involved in regulating eosinophil differentiation. In RhoH−/− mice, we observed reduced GATA-2 expression as well as accelerated eosinophil differentiation both in vitro and in vivo. Conversely, RhoH overexpression in bone marrow progenitors reduces eosinophil development in mixed bone marrow chimeras. These results highlight a novel negative regulatory role for RhoH in eosinophil differentiation, most likely in consequence of altered GATA-2 levels. Cell Death and Differentiation (2016) 23, 1961–1972; doi:10.1038/cdd.2016.73; published online 14 October 2016 Eosinophils are short-lived effector and regulatory cells that their interaction partners or competitors. For example, are increasingly recognized as having key roles in the while GATA-2 expression is required for the proper differentia- immune system, including maintenance of long-term antibody tion of several haematopoietic lineages, including eosino- responses and defence against bacteria and viruses.1,2 On phils,8 high GATA-2 expression inhibits HPC proliferation.9,10 the other hand, their potentially destructive nature becomes Similarly, overexpression of GATA-2 in progenitor cells in the apparent in a number of eosinophilic diseases such as presence of C/EBPα drives eosinophil differentiation, while hypereosinophilic syndromes (HES), diseases characterized it promotes mast cell/basophil generation in the absence of by eosinophil-mediated tissue damage and organ dysfunc- C/EBPα.11 Furthermore, while GATA-2 induces the expres- tion.3,4 Eosinophils contribute to disease pathology directly sion of eosinophil-derived neurotoxin, it also antagonizes and indirectly by releasing cytotoxic molecules and by sup- GATA-1-mediated expression of other eosinophil-related porting an ongoing inflammation.1 Besides HES, eosinophilia genes, including major basic protein.12–14 These apparently can be observed in a wide range of infectious, allergic and discordant findings highlight the need for a better under- autoimmune diseases, as well as in cancer.3,5 Given the standing of the molecular mechanisms controlling eosinophil increasing incidence of such diseases and the often unsa- differentiation. tisfactory treatment options available, it is of considerable The best studied cytokines promoting eosinophil develop- importance to better understand factors governing eosinophil ment are interleukin (IL)-3, IL-5, and granulocyte/macrophage biology under both normal and inflammatory conditions. colony-stimulating factor (GM-CSF).6 In addition to their role in Eosinophil numbers are largely regulated by their produc- eosinophilopoiesis, they also enhance eosinophil survival.15 tion in the bone marrow where they differentiate from myeloid Of these eosinophil hematopoietins, IL-5 is the most specific precursors.6 Lineage commitment is controlled by GATA-1, for the eosinophil lineage. Transgenic overexpression of IL-5 in GATA-2, PU.1 and c/EBP transcription factors. For instance, mice results in eosinophilia, whereas deletion of the IL-5 gene mice lacking GATA-1 or c/EBP expression are devoid of strongly reduces eosinophil counts in blood and lungs eosinophils, and transduction of hematopoietic progenitor following allergen challenge.6,16 Furthermore, many eosino- cells (HPC) with either of these genes promotes differentiation philic diseases are associated with increased IL-5 levels.3,17 into eosinophils.7 However, the situation is not always These findings together with the knowledge of the critical role straightforward. Lineage specification and maturation strongly of IL-5 for eosinophil generation and survival have prompted depend on the relative levels of these transcription factors and the development of therapeutic anti-IL-5 and anti-IL-5 receptor 1Institute of Pharmacology, University of Bern, Bern, Switzerland and 2Theodor Kocher Institute, University of Bern, Bern, Switzerland *Corresponding author: HU Simon, Institute of Pharmacology, University of Bern, Inselspital, INO-F, CH-3010 Bern, Switzerland. Tel: +41 31 632 3281; Fax: +41 31 632 4992; E-mail: [email protected] 3Current address: Novartis Institutes for Biomedical Research, Novartis Pharma AG, Basel, Switzerland 4Current address: Roche Pharma Research & Early Development, Roche Innovation Center Basel, Basel, Switzerland Abbreviations: ECP, eosinophil cationic protein; EDN, eosinophil-derived neurotoxin; FACS, fluorescence-activated cell sorting; Flt3L, Fms-related tyrosine kinase 3 ligand; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte/macrophage colony-stimulating factor; HES, hypereosinophilic syndrome; HPC, hematopoietic progenitor cells; 4-OHT, 4-hydroxytamoxifen; IL, interleukin; MEK, mitogen/extracellular signal-regulated kinase; PBMC, peripheral blood mononuclear cells; PCR, polymerase chain reaction; PI3K, phosphoinositide 3-kinase; ROCK, Rho-associated protein kinase; SCF, stem cell factor; STAT, signal transducer and activator of transcription; WT, wild-type Received 11.1.16; revised 16.6.16; accepted 27.6.16; Edited by P Salomoni; published online 14 October 2016 RhoH in eosinophil differentiation C Stoeckle et al 1962 n o i s s e r p x e Monocytes Neutrophils Eosinophils NK cells PBMC T cells Bcells A N 20 RhoH R m e 38 GAPDH v i t a l e R Eosinophils + IL-5 0 2h 3h 18h 24h -IL-5 IL-3 GM-CSF 20 RhoH 20 RhoH 38 GAPDH 38 GAPDH Healthy HES HES eosinophils + IL-5 donors patients 0 2h 3h 18h 24h 20 RhoH 86 20 RhoH 79 p-STAT3 38 GAPDH 38 GAPDH Figure 1 RhoH expression in peripheral blood eosinophils. Leukocyte subsets were isolated from peripheral blood and RhoH expression was measured by qPCR (a)or immunoblotting (b). Representative immunoblots of eosinophils from healthy donors stimulated with 10 ng/ml IL-5 (c) or 10 ng/ml of IL-5, IL-3 or GM-CSF for 3 h (d), freshly isolated eosinophils from healthy donors or HES patients (e), or IL-5 stimulated eosinophils from HES patients (f), are presented. Values in panel (a) represent means +/ − S.D. Data in panels (b–f) are representative for at least three independent experiments antibodies, which are currently being investigated in eosino- production in neutrophils,29 but its function in eosinophils is philic disorders.1,18 unknown. RhoH belongs to the family of small GTPases that are In this study, we report that RhoH deficiency promotes, and involved in diverse cellular processes, including signalling, RhoH overexpression reduces, eosinophil differentiation in vitro vesicle transport, migration, proliferation and differentiation. In and in vivo. Moreover, RhoH induces the developmentally contrast to most family members, RhoH is GTPase deficient important transcription factor GATA-2 through a Rho-associated and, therefore, its activity is assumed to be regulated at the protein kinase (ROCK)-dependent mechanism. In addition, level of protein expression rather than by switching between RhoH appears to promote cellular quiescence in a manner 10 active (GTP-bound) and inactive (GDP-bound) forms.19 RhoH similar to what has been reported for GATA-2. Furthermore, is exclusively expressed in the hematopoietic system and is our data suggest that IL-5 induces RhoH in immature mutated in a number of lymphomas and leukemias, but the eosinophils, a pathway that also remains functional following mechanisms by which dysregulated RhoH contributes to maturation. Taken together, our data suggest that IL-5 not only malignancy remain unclear. drives eosinophil differentiation, but also induces an inhibitory With respect to its physiological functions, RhoH is involved pathway limiting this process in which RhoH has a major role. in T-cell receptor (TCR) signalling, where it regulates the – subcellular localization of ZAP70 and LCK.20 22 Following Results TCR stimulation, RhoH is degraded.23 RhoH−/− mice have reduced T-cell numbers due to a failure of positive selection, RhoH is expressed in human eosinophils and upregu- 21,24 and impaired peripheral T-cell responses. This phenotype lated upon IL-5 stimulation. To investigate whether RhoH is is similar to that of two recently described natural RhoH- expressed in eosinophils under physiological conditions, we deficient siblings, in which impaired T-cell function resulted in isolated eosinophils from peripheral blood of healthy donors 25 susceptibility to certain viral infections. In mast cells, RhoH is and found that eosinophils expressed higher levels of RhoH critical for FcεRI-dependent
Load more

Recommended publications
  • Ran Activation Assay Kit
    Product Manual Ran Activation Assay Kit Catalog Number STA-409 20 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Ran, a 25 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include DNA synthesis, cell cycle progression, and translocation of RNA/proteins through the nuclear pore complex. Like other small GTPases, Ran regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ran binds specifically to RanBP1 to control downstream signaling cascades. Cell Biolabs’ Ran Activation Assay Kit utilizes RanBP1 Agarose beads to selectively isolate and pull- down the active form of Ran from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ran is detected by western blot analysis using an anti-Ran antibody. Cell Biolabs’ Ran Activation Assay Kit provides a simple and fast tool to monitor the activation of Ran. The kit includes easily identifiable RanBP1 Agarose beads (see Figure 1), pink in color, and a GTPase Immunoblot Positive Control for quick Ran identification. Each kit provides sufficient quantities to perform 20 assays. Figure 1: RanBP1 Agarose beads, in color, are easy to visualize, minimizing potential loss during washes and aspirations. 2 Assay Principle Related Products 1. STA-400: Pan-Ras Activation Assay Kit 2. STA-400-H: H-Ras Activation Assay Kit 3. STA-400-K: K-Ras Activation Assay Kit 4. STA-400-N: N-Ras Activation Assay Kit 5.
    [Show full text]
  • Cells RI-Dependent Signal Transduction in Mast Ε Rhoh Plays
    RhoH Plays Critical Roles in Fcε RI-Dependent Signal Transduction in Mast Cells This information is current as Hiroyo Oda, Manabu Fujimoto, Michael S. Patrick, Dai of October 2, 2021. Chida, Yoshinori Sato, Yoshinao Azuma, Hiroki Aoki, Takaya Abe, Harumi Suzuki and Mutsunori Shirai J Immunol 2009; 182:957-962; ; doi: 10.4049/jimmunol.182.2.957 http://www.jimmunol.org/content/182/2/957 Downloaded from References This article cites 45 articles, 13 of which you can access for free at: http://www.jimmunol.org/content/182/2/957.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication by guest on October 2, 2021 *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2009 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology RhoH Plays Critical Roles in Fc␧RI-Dependent Signal Transduction in Mast Cells1 Hiroyo Oda,* Manabu Fujimoto,† Michael S. Patrick,*‡ Dai Chida,* Yoshinori Sato,* Yoshinao Azuma,‡ Hiroki Aoki,§ Takaya Abe,¶ Harumi Suzuki,2* and Mutsunori Shirai‡ RhoH is an atypical small G protein with defective GTPase activity that is specifically expressed in hematopoietic lineage cells.
    [Show full text]
  • Characterization of Gf a Drosophila Trimeric G Protein Alpha Subunit
    Characterization of Gf a Drosophila trimeric G protein alpha subunit Naureen Quibria Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Graduate School of Arts and Sciences COLUMBIA UNIVERSITY 2012 © 2012 Naureen Quibria All rights reserved Abstract Characterization of Gf a Drosophila trimeric G-protein alpha subunit Naureen Quibria In the morphogenesis of tissue development, how coordination of patterning and growth achieve the correct organ size and shape is a principal question in biology. Efficient orchestrating mechanisms are required to achieve this and cells have developed sophisticated systems for reception and interpretation of the multitude of extracellular stimuli to which they are exposed. Plasma membrane receptors play a key role in the transmission of such signals. G-protein coupled receptors (GPCRs) are the largest class of cell surface receptors that respond to an enormous diversity of extracellular stimuli, and are critical mediators of cellular signal transduction in eukaryotic organisms. Signaling through GPCRs has been well characterized in many biological contexts. While they are a major class of signal transducers, there are not many defined instances where GPCRs have been implicated in the process of development to date. The Drosophila wing provides an ideal model system to elucidate and address the role of GPCRs in development, as its growth is regulated by a small number of conserved signaling pathways. In my thesis work, I address the role of a trimeric G alpha protein in Drosophila, Gαf, and what part it may play in development. In particular, I explore the role of Gαf as an alpha subunit of a trimeric complex, to determine what heptahelical receptors might act as its cognate receptor.
    [Show full text]
  • Expression of Rho Gtpases Family in Melanoma Cells and Its Influence on Cytoskeleton and Migration
    www.impactjournals.com/oncotarget/ Oncotarget, 2017, Vol. 8, (No. 18), pp: 30112-30122 Research Paper Expression of Rho GTPases family in melanoma cells and its influence on cytoskeleton and migration Si-Jian Wen1,2, Wei Zhang2, Na-Na Ni3, Qiong Wu2, Xiao-Po Wang2, You-Kun Lin1 Jian-Fang Sun2 1Department of Dermatology and Venerology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China 2Department of Pathology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China 3Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing 210042, China Correspondence to: You-Kun Lin, email: [email protected] Jian-Fang Sun, email: [email protected] Keywords: Rho GTPase, melanoma, cytoskeleton, cell migration Received: November 01, 2016 Accepted: January 11, 2017 Published: February 22, 2017 Copyright: Wen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Rho GTPases family members influenced the filopodia, lamellipodia, stress fiber and adhesion plaque of melanoma cells through regulating cytoskeleton recombination. The role of Rho GTPases family in the migration and invasion of melanoma and its molecular mechanism were explored. The morphological difference between three types of melanoma cells (M14, A375 and MV3) and human melanocyte (MC) was observed by the Hoffman microscope. Cells were stained by phalloidin labeled by rhodamine. The differences between 4 types of cells in filopodia, lamellipodia, stress fiber and adhesion plaque (microfilament is the main constituent) were observed under the super-high resolution microscope.
    [Show full text]
  • New Targets of Urocortin-Mediated Cardioprotection
    69 New targets of urocortin-mediated cardioprotection Sea´n P Barry1, Kevin M Lawrence4, James McCormick1, Surinder M Soond5, Mike Hubank2, Simon Eaton3, Ahila Sivarajah6, Tiziano M Scarabelli7, Richard A Knight1, Christoph Thiemermann6, David S Latchman1, Paul A Townsend8 and Anastasis Stephanou1 1Medical Molecular Biology Unit, 2Department of Molecular Haematology and 3Department of Surgery, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, UK 4Department of Cellular Pathology, St George’s, University of London, Cranmer Terrace, Tooting, London, SW17 0RE, UK 5School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK 6St Bartholomew’s and The Royal London School of Medicine and Dentistry, William Harvey Research Institute, Centre for Translational Medicine and Therapeutics, Queen Mary University of London, London, EC1M 7BQ, UK 7Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University School of Medicine, 22201 Moross Road, Detroit, Michigan 48336, USA 8Human Genetics Division, MP808, Southampton General Hospital, University of Southampton, Southampton SO16 6YD, UK (Correspondence should be addressed to S P Barry; Email: [email protected]) Abstract The urocortin (UCN) hormones UCN1 and UCN2 have been shown previously to confer significant protection against myocardial ischaemia/reperfusion (I/R) injury; however, the molecular mechanisms underlying their action are poorly understood. To further define the transcriptional effect of UCNs that underpins their cardioprotective activity, a microarray analysis was carried out using an in vivo rat coronary occlusion model of I/R injury. Infusion of UCN1 or UCN2 before the onset of reperfusion resulted in the differential regulation of 66 and 141 genes respectively, the majority of which have not been described previously.
    [Show full text]
  • 1714 Gene Comprehensive Cancer Panel Enriched for Clinically Actionable Genes with Additional Biologically Relevant Genes 400-500X Average Coverage on Tumor
    xO GENE PANEL 1714 gene comprehensive cancer panel enriched for clinically actionable genes with additional biologically relevant genes 400-500x average coverage on tumor Genes A-C Genes D-F Genes G-I Genes J-L AATK ATAD2B BTG1 CDH7 CREM DACH1 EPHA1 FES G6PC3 HGF IL18RAP JADE1 LMO1 ABCA1 ATF1 BTG2 CDK1 CRHR1 DACH2 EPHA2 FEV G6PD HIF1A IL1R1 JAK1 LMO2 ABCB1 ATM BTG3 CDK10 CRK DAXX EPHA3 FGF1 GAB1 HIF1AN IL1R2 JAK2 LMO7 ABCB11 ATR BTK CDK11A CRKL DBH EPHA4 FGF10 GAB2 HIST1H1E IL1RAP JAK3 LMTK2 ABCB4 ATRX BTRC CDK11B CRLF2 DCC EPHA5 FGF11 GABPA HIST1H3B IL20RA JARID2 LMTK3 ABCC1 AURKA BUB1 CDK12 CRTC1 DCUN1D1 EPHA6 FGF12 GALNT12 HIST1H4E IL20RB JAZF1 LPHN2 ABCC2 AURKB BUB1B CDK13 CRTC2 DCUN1D2 EPHA7 FGF13 GATA1 HLA-A IL21R JMJD1C LPHN3 ABCG1 AURKC BUB3 CDK14 CRTC3 DDB2 EPHA8 FGF14 GATA2 HLA-B IL22RA1 JMJD4 LPP ABCG2 AXIN1 C11orf30 CDK15 CSF1 DDIT3 EPHB1 FGF16 GATA3 HLF IL22RA2 JMJD6 LRP1B ABI1 AXIN2 CACNA1C CDK16 CSF1R DDR1 EPHB2 FGF17 GATA5 HLTF IL23R JMJD7 LRP5 ABL1 AXL CACNA1S CDK17 CSF2RA DDR2 EPHB3 FGF18 GATA6 HMGA1 IL2RA JMJD8 LRP6 ABL2 B2M CACNB2 CDK18 CSF2RB DDX3X EPHB4 FGF19 GDNF HMGA2 IL2RB JUN LRRK2 ACE BABAM1 CADM2 CDK19 CSF3R DDX5 EPHB6 FGF2 GFI1 HMGCR IL2RG JUNB LSM1 ACSL6 BACH1 CALR CDK2 CSK DDX6 EPOR FGF20 GFI1B HNF1A IL3 JUND LTK ACTA2 BACH2 CAMTA1 CDK20 CSNK1D DEK ERBB2 FGF21 GFRA4 HNF1B IL3RA JUP LYL1 ACTC1 BAG4 CAPRIN2 CDK3 CSNK1E DHFR ERBB3 FGF22 GGCX HNRNPA3 IL4R KAT2A LYN ACVR1 BAI3 CARD10 CDK4 CTCF DHH ERBB4 FGF23 GHR HOXA10 IL5RA KAT2B LZTR1 ACVR1B BAP1 CARD11 CDK5 CTCFL DIAPH1 ERCC1 FGF3 GID4 HOXA11 IL6R KAT5 ACVR2A
    [Show full text]
  • High Throughput Strategies Aimed at Closing the GAP in Our Knowledge of Rho Gtpase Signaling
    cells Review High Throughput strategies Aimed at Closing the GAP in Our Knowledge of Rho GTPase Signaling Manel Dahmene 1, Laura Quirion 2 and Mélanie Laurin 1,3,* 1 Oncology Division, CHU de Québec–Université Laval Research Center, Québec, QC G1V 4G2, Canada; [email protected] 2 Montréal Clinical Research Institute (IRCM), Montréal, QC H2W 1R7, Canada; [email protected] 3 Université Laval Cancer Research Center, Québec, QC G1R 3S3, Canada * Correspondence: [email protected] Received: 21 May 2020; Accepted: 7 June 2020; Published: 9 June 2020 Abstract: Since their discovery, Rho GTPases have emerged as key regulators of cytoskeletal dynamics. In humans, there are 20 Rho GTPases and more than 150 regulators that belong to the RhoGEF, RhoGAP, and RhoGDI families. Throughout development, Rho GTPases choregraph a plethora of cellular processes essential for cellular migration, cell–cell junctions, and cell polarity assembly. Rho GTPases are also significant mediators of cancer cell invasion. Nevertheless, to date only a few molecules from these intricate signaling networks have been studied in depth, which has prevented appreciation for the full scope of Rho GTPases’ biological functions. Given the large complexity involved, system level studies are required to fully grasp the extent of their biological roles and regulation. Recently, several groups have tackled this challenge by using proteomic approaches to map the full repertoire of Rho GTPases and Rho regulators protein interactions. These studies have provided in-depth understanding of Rho regulators specificity and have contributed to expand Rho GTPases’ effector portfolio. Additionally, new roles for understudied family members were unraveled using high throughput screening strategies using cell culture models and mouse embryos.
    [Show full text]
  • Open Questions: What About the 'Other' Rho Gtpases?
    Ridley BMC Biology (2016) 14:64 DOI 10.1186/s12915-016-0289-7 COMMENT Open Access Open questions: what about the ‘other’ Rho GTPases? Anne J. Ridley Abstract know if they interact with and/or regulate the activity of other family members. Indeed, by studying only RhoA, Rho GTPases have many and diverse roles in cell Rac1 and Cdc42, we are likely to be missing the real physiology, and some family members are very well functions of many GEFs and GAPs because their targets studied, including RhoA, Rac1 and Cdc42. But many in cells are among the other Rho GTPases. are relatively neglected, and fundamental questions Interestingly, four family members—Rnd1, Rnd2, Rnd3 about their mechanisms and functions remain open. and RhoH—are ‘atypical’, in that they are known to be constitutively GTP-bound and do not hydrolyse GTP: much less is known about how these family members Rho GTPases are household names for anyone who are regulated. RhoU and RhoV have high intrinsic GDP/ works on eukaryotic cell migration and their functions GTP exchange rates, so are unlikely to need GEFs for in cell migration, cell division and cell polarity are de- activation but could still be turned off by GAPs [1, 4]. scribed in most textbooks on cell biology. Yet most of For two other members, RhoBTB1 and RhoBTB2, the what we know about Rho GTPases comes from studying Rho domains are quite divergent in sequence from other a small subset of the many different family members, family members and they are unlikely to be regulated by namely RhoA, Rac1 and Cdc42.
    [Show full text]
  • Supp Table 6.Pdf
    Supplementary Table 6. Processes associated to the 2037 SCL candidate target genes ID Symbol Entrez Gene Name Process NM_178114 AMIGO2 adhesion molecule with Ig-like domain 2 adhesion NM_033474 ARVCF armadillo repeat gene deletes in velocardiofacial syndrome adhesion NM_027060 BTBD9 BTB (POZ) domain containing 9 adhesion NM_001039149 CD226 CD226 molecule adhesion NM_010581 CD47 CD47 molecule adhesion NM_023370 CDH23 cadherin-like 23 adhesion NM_207298 CERCAM cerebral endothelial cell adhesion molecule adhesion NM_021719 CLDN15 claudin 15 adhesion NM_009902 CLDN3 claudin 3 adhesion NM_008779 CNTN3 contactin 3 (plasmacytoma associated) adhesion NM_015734 COL5A1 collagen, type V, alpha 1 adhesion NM_007803 CTTN cortactin adhesion NM_009142 CX3CL1 chemokine (C-X3-C motif) ligand 1 adhesion NM_031174 DSCAM Down syndrome cell adhesion molecule adhesion NM_145158 EMILIN2 elastin microfibril interfacer 2 adhesion NM_001081286 FAT1 FAT tumor suppressor homolog 1 (Drosophila) adhesion NM_001080814 FAT3 FAT tumor suppressor homolog 3 (Drosophila) adhesion NM_153795 FERMT3 fermitin family homolog 3 (Drosophila) adhesion NM_010494 ICAM2 intercellular adhesion molecule 2 adhesion NM_023892 ICAM4 (includes EG:3386) intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)adhesion NM_001001979 MEGF10 multiple EGF-like-domains 10 adhesion NM_172522 MEGF11 multiple EGF-like-domains 11 adhesion NM_010739 MUC13 mucin 13, cell surface associated adhesion NM_013610 NINJ1 ninjurin 1 adhesion NM_016718 NINJ2 ninjurin 2 adhesion NM_172932 NLGN3 neuroligin
    [Show full text]
  • Supplementary Table 1
    Supplementary Table 1. 492 genes are unique to 0 h post-heat timepoint. The name, p-value, fold change, location and family of each gene are indicated. Genes were filtered for an absolute value log2 ration 1.5 and a significance value of p ≤ 0.05. Symbol p-value Log Gene Name Location Family Ratio ABCA13 1.87E-02 3.292 ATP-binding cassette, sub-family unknown transporter A (ABC1), member 13 ABCB1 1.93E-02 −1.819 ATP-binding cassette, sub-family Plasma transporter B (MDR/TAP), member 1 Membrane ABCC3 2.83E-02 2.016 ATP-binding cassette, sub-family Plasma transporter C (CFTR/MRP), member 3 Membrane ABHD6 7.79E-03 −2.717 abhydrolase domain containing 6 Cytoplasm enzyme ACAT1 4.10E-02 3.009 acetyl-CoA acetyltransferase 1 Cytoplasm enzyme ACBD4 2.66E-03 1.722 acyl-CoA binding domain unknown other containing 4 ACSL5 1.86E-02 −2.876 acyl-CoA synthetase long-chain Cytoplasm enzyme family member 5 ADAM23 3.33E-02 −3.008 ADAM metallopeptidase domain Plasma peptidase 23 Membrane ADAM29 5.58E-03 3.463 ADAM metallopeptidase domain Plasma peptidase 29 Membrane ADAMTS17 2.67E-04 3.051 ADAM metallopeptidase with Extracellular other thrombospondin type 1 motif, 17 Space ADCYAP1R1 1.20E-02 1.848 adenylate cyclase activating Plasma G-protein polypeptide 1 (pituitary) receptor Membrane coupled type I receptor ADH6 (includes 4.02E-02 −1.845 alcohol dehydrogenase 6 (class Cytoplasm enzyme EG:130) V) AHSA2 1.54E-04 −1.6 AHA1, activator of heat shock unknown other 90kDa protein ATPase homolog 2 (yeast) AK5 3.32E-02 1.658 adenylate kinase 5 Cytoplasm kinase AK7
    [Show full text]
  • Molecular Signatures Differentiate Immune States in Type 1 Diabetes Families
    Page 1 of 65 Diabetes Molecular signatures differentiate immune states in Type 1 diabetes families Yi-Guang Chen1, Susanne M. Cabrera1, Shuang Jia1, Mary L. Kaldunski1, Joanna Kramer1, Sami Cheong2, Rhonda Geoffrey1, Mark F. Roethle1, Jeffrey E. Woodliff3, Carla J. Greenbaum4, Xujing Wang5, and Martin J. Hessner1 1The Max McGee National Research Center for Juvenile Diabetes, Children's Research Institute of Children's Hospital of Wisconsin, and Department of Pediatrics at the Medical College of Wisconsin Milwaukee, WI 53226, USA. 2The Department of Mathematical Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA. 3Flow Cytometry & Cell Separation Facility, Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA. 4Diabetes Research Program, Benaroya Research Institute, Seattle, WA, 98101, USA. 5Systems Biology Center, the National Heart, Lung, and Blood Institute, the National Institutes of Health, Bethesda, MD 20824, USA. Corresponding author: Martin J. Hessner, Ph.D., The Department of Pediatrics, The Medical College of Wisconsin, Milwaukee, WI 53226, USA Tel: 011-1-414-955-4496; Fax: 011-1-414-955-6663; E-mail: [email protected]. Running title: Innate Inflammation in T1D Families Word count: 3999 Number of Tables: 1 Number of Figures: 7 1 For Peer Review Only Diabetes Publish Ahead of Print, published online April 23, 2014 Diabetes Page 2 of 65 ABSTRACT Mechanisms associated with Type 1 diabetes (T1D) development remain incompletely defined. Employing a sensitive array-based bioassay where patient plasma is used to induce transcriptional responses in healthy leukocytes, we previously reported disease-specific, partially IL-1 dependent, signatures associated with pre and recent onset (RO) T1D relative to unrelated healthy controls (uHC).
    [Show full text]
  • Rho Gtpases of the Rhobtb Subfamily and Tumorigenesis1
    Acta Pharmacol Sin 2008 Mar; 29 (3): 285–295 Invited review Rho GTPases of the RhoBTB subfamily and tumorigenesis1 Jessica BERTHOLD2, Kristína SCHENKOVÁ2, Francisco RIVERO2,3,4 2Centers for Biochemistry and Molecular Medicine, University of Cologne, Cologne, Germany; 3The Hull York Medical School, University of Hull, Hull HU6 7RX, UK Key words Abstract Rho guanosine triphosphatase; RhoBTB; RhoBTB proteins constitute a subfamily of atypical members within the Rho fa- DBC2; cullin; neoplasm mily of small guanosine triphosphatases (GTPases). Their most salient feature 1This work was supported by grants from the is their domain architecture: a GTPase domain (in most cases, non-functional) Center for Molecular Medicine Cologne, the is followed by a proline-rich region, a tandem of 2 broad-complex, tramtrack, Deutsche Forschungsgemeinschaft, and the bric à brac (BTB) domains, and a conserved C-terminal region. In humans, Köln Fortune Program of the Medical Faculty, University of Cologne. the RhoBTB subfamily consists of 3 isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. Orthologs are present in several other eukaryotes, such as Drosophi- 4 Correspondence to Dr Francisco RIVERO. la and Dictyostelium, but have been lost in plants and fungi. Interest in RhoBTB Phn 49-221-478-6987. Fax 49-221-478-6979. arose when RHOBTB2 was identified as the gene homozygously deleted in E-mail [email protected] breast cancer samples and was proposed as a candidate tumor suppressor gene, a property that has been extended to RHOBTB1. The functions of RhoBTB pro- Received 2007-11-17 Accepted 2007-12-16 teins have not been defined yet, but may be related to the roles of BTB domains in the recruitment of cullin3, a component of a family of ubiquitin ligases.
    [Show full text]