Spatial RNA Sequencing Identifies Robust Markers Of
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Spatially Heterogeneous Choroid Plexus Transcriptomes Encode Positional Identity and Contribute to Regional CSF Production
The Journal of Neuroscience, March 25, 2015 • 35(12):4903–4916 • 4903 Development/Plasticity/Repair Spatially Heterogeneous Choroid Plexus Transcriptomes Encode Positional Identity and Contribute to Regional CSF Production Melody P. Lun,1,3 XMatthew B. Johnson,2 Kevin G. Broadbelt,1 Momoko Watanabe,4 Young-jin Kang,4 Kevin F. Chau,1 Mark W. Springel,1 Alexandra Malesz,1 Andre´ M.M. Sousa,5 XMihovil Pletikos,5 XTais Adelita,1,6 Monica L. Calicchio,1 Yong Zhang,7 Michael J. Holtzman,7 Hart G.W. Lidov,1 XNenad Sestan,5 Hanno Steen,1 XEdwin S. Monuki,4 and Maria K. Lehtinen1 1Department of Pathology, and 2Division of Genetics, Boston Children’s Hospital, Boston, Massachusetts 02115, 3Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, 4Department of Pathology and Laboratory Medicine, University of California Irvine School of Medicine, Irvine, California 92697, 5Department of Neurobiology and Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, Connecticut 06510, 6Department of Biochemistry, Federal University of Sa˜o Paulo, Sa˜o Paulo 04039, Brazil, and 7Pulmonary and Critical Care Medicine, Department of Medicine, Washington University, St Louis, Missouri 63110 A sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors into the CSF. To evaluate whether differences in the CSF proteome across ventricles arise, in part, from regional differences in choroid plexus gene expression, we defined the transcriptome of lateral ventricle (telencephalic) versus fourth ventricle (hindbrain) choroid plexus. We find that positional identitiesofmouse,macaque,andhumanchoroidplexiderivefromgeneexpressiondomainsthatparalleltheiraxialtissuesoforigin.We thenshowthatmolecularheterogeneitybetweentelencephalicandhindbrainchoroidplexicontributestoregion-specific,age-dependent protein secretion in vitro. -
Genome-Wide Screening Identifies a KCNIP1 Copy Number Variant As a Genetic Predictor for Atrial Fibrillation
ARTICLE Received 21 Oct 2014 | Accepted 16 Nov 2015 | Published 2 Feb 2016 DOI: 10.1038/ncomms10190 OPEN Genome-wide screening identifies a KCNIP1 copy number variant as a genetic predictor for atrial fibrillation Chia-Ti Tsai1,2,3, Chia-Shan Hsieh4,5, Sheng-Nan Chang2,3, Eric Y. Chuang4,5, Kwo-Chang Ueng6,7, Chin-Feng Tsai6,7, Tsung-Hsien Lin8, Cho-Kai Wu1, Jen-Kuang Lee1, Lian-Yu Lin1, Yi-Chih Wang1, Chih-Chieh Yu1, Ling-Ping Lai1, Chuen-Den Tseng1, Juey-Jen Hwang1, Fu-Tien Chiang1,9 & Jiunn-Lee Lin1 Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia. Previous genome- wide association studies had identified single-nucleotide polymorphisms in several genomic regions to be associated with AF. In human genome, copy number variations (CNVs) are known to contribute to disease susceptibility. Using a genome-wide multistage approach to identify AF susceptibility CNVs, we here show a common 4,470-bp diallelic CNV in the first intron of potassium interacting channel 1 gene (KCNIP1) is strongly associated with AF in Taiwanese populations (odds ratio ¼ 2.27 for insertion allele; P ¼ 6.23 Â 10 À 24). KCNIP1 insertion is associated with higher KCNIP1 mRNA expression. KCNIP1-encoded protein potassium interacting channel 1 (KCHIP1) is physically associated with potassium Kv channels and modulates atrial transient outward current in cardiac myocytes. Overexpression of KCNIP1 results in inducible AF in zebrafish. In conclusions, a common CNV in KCNIP1 gene is a genetic predictor of AF risk possibly pointing to a functional pathway. 1 Division of Cardiology, Department of Internal Medicine, National Taiwan University College of Medicine and Hospital, No. -
DNA Methylation Associated with Postpartum Depressive Symptoms Overlaps Findings from a Genome-Wide Association Meta-Analysis of Depression Dana M
Lapato et al. Clinical Epigenetics (2019) 11:169 https://doi.org/10.1186/s13148-019-0769-z RESEARCH Open Access DNA methylation associated with postpartum depressive symptoms overlaps findings from a genome-wide association meta-analysis of depression Dana M. Lapato1,2* , Roxann Roberson-Nay2,3, Robert M. Kirkpatrick2,3, Bradley T. Webb2,3, Timothy P. York1,2,4† and Patricia A. Kinser5† Abstract Background: Perinatal depressive symptoms have been linked to adverse maternal and infant health outcomes. The etiology associated with perinatal depressive psychopathology is poorly understood, but accumulating evidence suggests that understanding inter-individual differences in DNA methylation (DNAm) patterning may provide insight regarding the genomic regions salient to the risk liability of perinatal depressive psychopathology. Results: Genome-wide DNAm was measured in maternal peripheral blood using the Infinium MethylationEPIC microarray. Ninety-two participants (46% African-American) had DNAm samples that passed all quality control metrics, and all participants were within 7 months of delivery. Linear models were constructed to identify differentially methylated sites and regions, and permutation testing was utilized to assess significance. Differentially methylated regions (DMRs) were defined as genomic regions of consistent DNAm change with at least two probes within 1 kb of each other. Maternal age, current smoking status, estimated cell-type proportions, ancestry-relevant principal components, days since delivery, and chip position served as covariates to adjust for technical and biological factors. Current postpartum depressive symptoms were measured using the Edinburgh Postnatal Depression Scale. Ninety-eight DMRs were significant (false discovery rate < 5%) and overlapped 92 genes. Three of the regions overlap loci from the latest Psychiatric Genomics Consortium meta-analysis of depression. -
Dimethylation of Histone 3 Lysine 9 Is Sensitive to the Epileptic Activity
1368 MOLECULAR MEDICINE REPORTS 17: 1368-1374, 2018 Dimethylation of Histone 3 Lysine 9 is sensitive to the epileptic activity, and affects the transcriptional regulation of the potassium channel Kcnj10 gene in epileptic rats SHAO-PING ZHANG1,2*, MAN ZHANG1*, HONG TAO1, YAN LUO1, TAO HE3, CHUN-HUI WANG3, XIAO-CHENG LI3, LING CHEN1,3, LIN-NA ZHANG1, TAO SUN2 and QI-KUAN HU1-3 1Department of Physiology; 2Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Ningxia Medical University; 3General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China Received February 18, 2017; Accepted September 13, 2017 DOI: 10.3892/mmr.2017.7942 Abstract. Potassium channels can be affected by epileptic G9a by 2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-di- seizures and serve a crucial role in the pathophysiology of methoxy-N-(1-(phenyl-methyl)-4-piperidinyl)-4-quinazolinamine epilepsy. Dimethylation of histone 3 lysine 9 (H3K9me2) and tri-hydrochloride hydrate (bix01294) resulted in upregulation its enzyme euchromatic histone-lysine N-methyltransferase 2 of the expression of Kir4.1 proteins. The present study demon- (G9a) are the major epigenetic modulators and are associated strated that H3K9me2 and G9a are sensitive to epileptic seizure with gene silencing. Insight into whether H3K9me2 and G9a activity during the acute phase of epilepsy and can affect the can respond to epileptic seizures and regulate expression of transcriptional regulation of the Kcnj10 channel. genes encoding potassium channels is the main purpose of the present study. A total of 16 subtypes of potassium channel Introduction genes in pilocarpine-modelled epileptic rats were screened by reverse transcription-quantitative polymerase chain reac- Epilepsies are disorders of neuronal excitability, characterized tion, and it was determined that the expression ATP-sensitive by spontaneous and recurrent seizures. -
Pflugers Final
CORE Metadata, citation and similar papers at core.ac.uk Provided by Serveur académique lausannois A comprehensive analysis of gene expression profiles in distal parts of the mouse renal tubule. Sylvain Pradervand2, Annie Mercier Zuber1, Gabriel Centeno1, Olivier Bonny1,3,4 and Dmitri Firsov1,4 1 - Department of Pharmacology and Toxicology, University of Lausanne, 1005 Lausanne, Switzerland 2 - DNA Array Facility, University of Lausanne, 1015 Lausanne, Switzerland 3 - Service of Nephrology, Lausanne University Hospital, 1005 Lausanne, Switzerland 4 – these two authors have equally contributed to the study to whom correspondence should be addressed: Dmitri FIRSOV Department of Pharmacology and Toxicology, University of Lausanne, 27 rue du Bugnon, 1005 Lausanne, Switzerland Phone: ++ 41-216925406 Fax: ++ 41-216925355 e-mail: [email protected] and Olivier BONNY Department of Pharmacology and Toxicology, University of Lausanne, 27 rue du Bugnon, 1005 Lausanne, Switzerland Phone: ++ 41-216925417 Fax: ++ 41-216925355 e-mail: [email protected] 1 Abstract The distal parts of the renal tubule play a critical role in maintaining homeostasis of extracellular fluids. In this review, we present an in-depth analysis of microarray-based gene expression profiles available for microdissected mouse distal nephron segments, i.e., the distal convoluted tubule (DCT) and the connecting tubule (CNT), and for the cortical portion of the collecting duct (CCD) (Zuber et al., 2009). Classification of expressed transcripts in 14 major functional gene categories demonstrated that all principal proteins involved in maintaining of salt and water balance are represented by highly abundant transcripts. However, a significant number of transcripts belonging, for instance, to categories of G protein-coupled receptors (GPCR) or serine-threonine kinases exhibit high expression levels but remain unassigned to a specific renal function. -
New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System
brain sciences Review New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System Krisztina Kelemen * and Tibor Szilágyi Department of Physiology, Doctoral School, Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Târgu Mures, , Romania; [email protected] * Correspondence: [email protected]; Tel.: +40-746-248064 Abstract: Although Ca2+ ion plays an essential role in cellular physiology, calcium-binding proteins (CaBPs) were long used for mainly as immunohistochemical markers of specific cell types in different regions of the central nervous system. They are a heterogeneous and wide-ranging group of proteins. Their function was studied intensively in the last two decades and a tremendous amount of informa- tion was gathered about them. Girard et al. compiled a comprehensive list of the gene-expression profiles of the entire EF-hand gene superfamily in the murine brain. We selected from this database those CaBPs which are related to information processing and/or neuronal signalling, have a Ca2+- buffer activity, Ca2+-sensor activity, modulator of Ca2+-channel activity, or a yet unknown function. In this way we created a gene function-based selection of the CaBPs. We cross-referenced these findings with publicly available, high-quality RNA-sequencing and in situ hybridization databases (Human Protein Atlas (HPA), Brain RNA-seq database and Allen Brain Atlas integrated into the HPA) and created gene expression heat maps of the regional and cell type-specific expression levels of the selected CaBPs. This represents a useful tool to predict and investigate different expression patterns and functions of the less-known CaBPs of the central nervous system. -
N-Methyl-D-Aspartate Receptors Mediate Activity-Dependent Down
Lee et al. Molecular Brain (2015) 8:4 DOI 10.1186/s13041-015-0094-1 RESEARCH Open Access N-methyl-D-aspartate receptors mediate activity- dependent down-regulation of potassium channel genes during the expression of homeostatic intrinsic plasticity Kwan Young Lee1†, Sara E Royston2,3†, Max O Vest1, Daniel J Ley1, Seungbae Lee1, Eric C Bolton1 and Hee Jung Chung1,2* Abstract Background: Homeostatic intrinsic plasticity encompasses the mechanisms by which neurons stabilize their excitability in response to prolonged and destabilizing changes in global activity. However, the milieu of molecular players responsible for these regulatory mechanisms is largely unknown. Results: Using whole-cell patch clamp recording and unbiased gene expression profiling in rat dissociated hippocampal neurons cultured at high density, we demonstrate here that chronic activity blockade induced by the sodium channel blocker tetrodotoxin leads to a homeostatic increase in action potential firing and down-regulation of potassium channel genes. In addition, chronic activity blockade reduces total potassium current, as well as protein expression and current of voltage-gated Kv1 and Kv7 potassium channels, which are critical regulators of action potential firing. Importantly, inhibition of N-Methyl-D-Aspartate receptors alone mimics the effects of tetrodotoxin, including the elevation in firing frequency and reduction of potassium channel gene expression and current driven by activity blockade, whereas inhibition of L-type voltage-gated calcium channels has no effect. Conclusions: Collectively, our data suggest that homeostatic intrinsic plasticity induced by chronic activity blockade is accomplished in part by decreased calcium influx through N-Methyl-D-Aspartate receptors and subsequent transcriptional down-regulation of potassium channel genes. -
Oestrogen Receptor Β Mediates the Actions of Bisphenol-A on Ion Channel Expression in Mouse Pancreatic Beta Cells
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by idUS. Depósito de Investigación Universidad de Sevilla Diabetologia (2019) 62:1667–1680 https://doi.org/10.1007/s00125-019-4925-y ARTICLE Oestrogen receptor β mediates the actions of bisphenol-A on ion channel expression in mouse pancreatic beta cells Juan Martinez-Pinna1,2,3 & Laura Marroqui1,2,4 & Abdelkrim Hmadcha4,5 & Javier Lopez-Beas4,5 & Sergi Soriano1,2,3 & Sabrina Villar-Pazos1,2,4 & Paloma Alonso-Magdalena1,2,4 & Reinaldo S. Dos Santos1,2,4 & Ivan Quesada1,2,4 & Franz Martin4,5 & Bernat Soria4,5 & Jan-Åke Gustafsson6,7 & Angel Nadal1,2,4 Received: 18 February 2019 /Accepted: 26 April 2019 /Published online: 27 June 2019 # Springer-Verlag GmbH Germany, part of Springer Nature 2019 Abstract Aims/hypothesis Bisphenol-A (BPA) is a widespread endocrine-disrupting chemical that has been associated with type 2 dia- betes development. Low doses of BPA modify pancreatic beta cell function and induce insulin resistance; some of these effects are mediated via activation of oestrogen receptors α (ERα)andβ (ERβ). Here we investigated whether low doses of BPA regulate the expression and function of ion channel subunits involved in beta cell function. Methods Microarray gene profiling of isolated islets from vehicle- and BPA-treated (100 μg/kg per day for 4 days) mice was performed using Affymetrix GeneChip Mouse Genome 430.2 Array. Expression level analysis was performed using the nor- malisation method based on the processing algorithm ‘robust multi-array average’. Whole islets or dispersed islets from C57BL/ 6J or oestrogen receptor β (ERβ) knockout (Erβ−/−) mice were treated with vehicle or BPA (1 nmol/l) for 48 h. -
Competing Endogenous RNA Regulatory Network in Papillary Thyroid Carcinoma
MOLECULAR MEDICINE REPORTS 18: 695-704, 2018 Competing endogenous RNA regulatory network in papillary thyroid carcinoma SHOUHUA CHEN1, XIAOBIN FAN2, HE GU1, LILI ZHANG1 and WENHUA ZHAO3 Departments of 1Breast and Thyroid Surgery, 2Operation Room and 3Oncology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China Received March 13, 2017; Accepted November 15, 2017 DOI: 10.3892/mmr.2018.9009 Abstract. The present study aimed to screen all types of RNAs receptor interaction’ pathways. Hox transcript antisense RNA, involved in the development of papillary thyroid carcinoma miRNA-206 and kallikrein-related peptidase 10 were nodes (PTC). RNA-sequencing data of PTC and normal samples were in the ceRNA regulatory network of the selected feature gene, used for screening differentially expressed (DE) microRNAs and they may serve import roles in the development of PTC. (DE-miRNAs), long non-coding RNAs (DE-lncRNAs) and genes (DEGs). Subsequently, lncRNA-miRNA, miRNA-gene Introduction (that is, miRNA-mRNA) and gene-gene interaction pairs were extracted and used to construct regulatory networks. Thyroid carcinomas originate from follicular or parafol- Feature genes in the miRNA-mRNA network were identi- licular thyroid cells (1). Papillary thyroid carcinomas (PTC) fied by topological analysis and recursive feature elimination are the most frequent histologic type of thyroid carcinoma, analysis. A support vector machine (SVM) classifier was built and account for 75-85% of reported cases (2). The incidence using 15 feature genes, and its classification effect was vali- rate of PTC has increased rapidly in recent years, and 5-10% dated using two microarray data sets that were downloaded of patients with PTC experience a particularly aggressive from the Gene Expression Omnibus (GEO) database. -
Robles JTO Supplemental Digital Content 1
Supplementary Materials An Integrated Prognostic Classifier for Stage I Lung Adenocarcinoma based on mRNA, microRNA and DNA Methylation Biomarkers Ana I. Robles1, Eri Arai2, Ewy A. Mathé1, Hirokazu Okayama1, Aaron Schetter1, Derek Brown1, David Petersen3, Elise D. Bowman1, Rintaro Noro1, Judith A. Welsh1, Daniel C. Edelman3, Holly S. Stevenson3, Yonghong Wang3, Naoto Tsuchiya4, Takashi Kohno4, Vidar Skaug5, Steen Mollerup5, Aage Haugen5, Paul S. Meltzer3, Jun Yokota6, Yae Kanai2 and Curtis C. Harris1 Affiliations: 1Laboratory of Human Carcinogenesis, NCI-CCR, National Institutes of Health, Bethesda, MD 20892, USA. 2Division of Molecular Pathology, National Cancer Center Research Institute, Tokyo 104-0045, Japan. 3Genetics Branch, NCI-CCR, National Institutes of Health, Bethesda, MD 20892, USA. 4Division of Genome Biology, National Cancer Center Research Institute, Tokyo 104-0045, Japan. 5Department of Chemical and Biological Working Environment, National Institute of Occupational Health, NO-0033 Oslo, Norway. 6Genomics and Epigenomics of Cancer Prediction Program, Institute of Predictive and Personalized Medicine of Cancer (IMPPC), 08916 Badalona (Barcelona), Spain. List of Supplementary Materials Supplementary Materials and Methods Fig. S1. Hierarchical clustering of based on CpG sites differentially-methylated in Stage I ADC compared to non-tumor adjacent tissues. Fig. S2. Confirmatory pyrosequencing analysis of DNA methylation at the HOXA9 locus in Stage I ADC from a subset of the NCI microarray cohort. 1 Fig. S3. Methylation Beta-values for HOXA9 probe cg26521404 in Stage I ADC samples from Japan. Fig. S4. Kaplan-Meier analysis of HOXA9 promoter methylation in a published cohort of Stage I lung ADC (J Clin Oncol 2013;31(32):4140-7). Fig. S5. Kaplan-Meier analysis of a combined prognostic biomarker in Stage I lung ADC. -
Gene Ontology Supplemental Tables
Gene Ontology Supplemental Tables Summary This file contains the full results for the combined gene set enrichment analysis and the DMR and DMP only enrichment analyses performed with clusterProfiler. DMP enrichment tests also were performed with methylGSA. clusterProfiler DMR & DMP Ontology Description GeneRatio BgRatio pvalue qvalue geneID BP cognition 11/136 284/17397 0.0000 0.038 TTC8/ADORA1/CNTNAP2/MEF2C/MEIS2/NTSR1/PAFAH1B1/ ADGRB3/RASGRF1/SLC6A4/SYNGAP1 BP learning or 9/136 246/17397 0.0001 0.114 CNTNAP2/MEF2C/MEIS2/NTSR1/PAFAH1B1/ADGRB3/RASGRF1/ memory SLC6A4/SYNGAP1 BP detection of 3/136 15/17397 0.0002 0.114 ADORA1/ARRB2/NTSR1 temperature stimulus i BP detection of 3/136 15/17397 0.0002 0.114 ADORA1/ARRB2/NTSR1 temperature stimulus i BP dendrite 8/136 216/17397 0.0003 0.114 CTNND2/COBL/MAP2/MEF2C/PAFAH1B1/ADGRB3/KLF7/SYNGAP1 development BP detection of 3/136 19/17397 0.0004 0.114 ADORA1/ARRB2/NTSR1 temperature stimulus BP modulation of 11/136 417/17397 0.0004 0.114 ADORA1/SYT9/ARRB2/MEF2C/NTSR1/RASGRF1/SLC6A4/TMEM108/ chemical YWHAG/SYNGAP1/CLSTN3 synaptic tra BP regulation of 11/136 418/17397 0.0004 0.114 ADORA1/SYT9/ARRB2/MEF2C/NTSR1/RASGRF1/SLC6A4/TMEM108/ trans-synaptic YWHAG/SYNGAP1/CLSTN3 signal BP platelet 3/136 20/17397 0.0005 0.114 ZFPM1/MEF2C/MYH9 formation BP establishment 6/136 128/17397 0.0005 0.114 SDCCAG8/SH3BP1/MAP2/MYH9/PAFAH1B1/FRMD4A of cell polarity BP actin filament- 15/136 723/17397 0.0005 0.114 ABI2/ADORA1/DIAPH2/COBL/SH3BP1/KCNJ5/MEF2C/MYH9/NEB/ based process PAFAH1B1/PLS3/LURAP1/ACTN4/MYOM2/TBCK BP platelet -
Ca 2+ -Dependent Transcriptional Repressors KCNIP and Regulation
Ca 2+ -Dependent Transcriptional Repressors KCNIP and Regulation of Prognosis Genes in Glioblastoma Isabelle Néant, Jacques Haiech, Marie-Claude Kilhoffer, Francisco Aulestia, Marc Moreau, Catherine Leclerc To cite this version: Isabelle Néant, Jacques Haiech, Marie-Claude Kilhoffer, Francisco Aulestia, Marc Moreau, et al.. Ca 2+ -Dependent Transcriptional Repressors KCNIP and Regulation of Prognosis Genes in Glioblas- toma. Frontiers in Molecular Neuroscience, Frontiers Media, 2018, 10.3389/fnmol.2018.00472. hal- 02329159 HAL Id: hal-02329159 https://hal.archives-ouvertes.fr/hal-02329159 Submitted on 23 Oct 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. fnmol-11-00472 December 16, 2018 Time: 13:6 # 1 REVIEW published: 18 December 2018 doi: 10.3389/fnmol.2018.00472 Ca2C-Dependent Transcriptional Repressors KCNIP and Regulation of Prognosis Genes in Glioblastoma Isabelle Néant1, Jacques Haiech2, Marie-Claude Kilhoffer2, Francisco J. Aulestia3, Marc Moreau1 and Catherine Leclerc1* 1 Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI), CNRS, UPS, Université de Toulouse, Toulouse, France, 2 Laboratoire d’Excellence Medalis, CNRS, LIT UMR 7200, Université de Strasbourg, Strasbourg, France, 3 Department of Basic Science and Craniofacial Biology, NYU College of Dentistry, New York, NY, United States Glioblastomas (GBMs) are the most aggressive and lethal primary astrocytic tumors in adults, with very poor prognosis.