MED26 Antibody

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MED26 Antibody Product Datasheet MED26 Antibody Catalog No: #33633 Package Size: #33633-1 50ul #33633-2 100ul Orders: [email protected] Support: [email protected] Description Product Name MED26 Antibody Host Species Rabbit Clonality Polyclonal Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Applications WB Species Reactivity Hu Ms Specificity The antibody detects endogenous levels of total MED26 protein. Immunogen Type Peptide Immunogen Description Synthesized peptide derived from N-terminal of human MED26. Target Name MED26 Other Names ARC70; CRSP complex 7; CRSP70; MED26; activator-recruited cofactor 70 kDa component Accession No. Swiss-Prot: O95402NCBI Gene ID: 9441 SDS-PAGE MW 65kd Concentration 1.0mg/ml Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Store at -20°C Application Details Western blotting: 1:500~1:3000 Images Western blot analysis of extracts from LOVO cells, using MED26 antibody #33633. Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ryu S., Nature 397:446-450(1999). Naeaer A.M., Nature 398:828-832(1999). Sato S., Mol. Cell 14:685-691(2004). Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.
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  • Technic Al Data Sheet
    PRODUCT NAME Med26 polyclonal antibody Other names: Transcriptional coactivator CRSP70, ARC70 Cat. No. C15410073 (pAb-073-050) Type: Polyclonal Size: 50 μg/ 25 μl Lot #: 001 Source: Rabbit Concentration: 2.0 μg/μl Description: Polyclonal antibody raised in rabbit against human Med26 (Mediator of RNA polymerase II transcription subunit 26), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein. TECHNICAL DATA SHEET DATA TECHNICAL Specificity: Human: positive Other species: not tested Applications Suggested dilution References Western blotting 1:1,000 Fig 1 Purity: Protein G purified, polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. Storage: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures. Last data sheet update: March 17, 2010 Target description Med26 (UniProt/Swiss-Prot entry O95402) is a component of the Mediator complex, a coactivator involved in the regulation of transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex forms a bridge between gene-specific regulatory proteins and the RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with these regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and general transcription factors. Europe - Diagenode s.a. / [email protected] / [email protected] // North America - Diagenode Inc. / [email protected] / [email protected] www.diagenode.com TECHNICAL DATA SHEET DATA TECHNICAL Figure 1 Western blot analysis using the Diagenode antibody directed against Med26 Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human Med26 (Cat.
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  • Mutual Exclusivity of MED12/MED12L, MED13/13L, and CDK8/19 Paralogs Revealed Within the CDK-Mediator Kinase Module Danette L
    ics om & B te i ro o P in f f o o r l m a Journal of a n t r i Daniels et al., J Proteomics Bioinform 2013, S2 c u s o J DOI: 10.4172/jpb.S2-004 ISSN: 0974-276X Proteomics & Bioinformatics Research Article Article OpenOpen Access Access Mutual Exclusivity of MED12/MED12L, MED13/13L, and CDK8/19 Paralogs Revealed within the CDK-Mediator Kinase Module Danette L. Daniels1*, Michael Ford2*, Marie K. Schwinn1, Hélène Benink1, Matthew D. Galbraith3,4, Ravi Amunugama2, Richard Jones2, David Allen2, Noriko Okazaki5, Hisashi Yamakawa5, Futaba Miki5, Takahiro Nagase5, Joaquín M. Espinosa3,4, and Marjeta Urh1 1Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA 2MSBioworks LLC, 3950 Varsity Drive, Ann Arbor, MI 48108, USA 3Howard Hughes Medical Institute, USA 4Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, 80309, USA 5Kazusa DNA Research Institute, Kisarazu 292-0818, Japan Abstract The macromolecular complex Mediator plays important roles in regulation of RNA Polymerase II (RNAPII) activity by DNA-binding proteins, non-coding RNAs, and chromatin. Structural and biochemical studies have shown that human Mediator exists in two forms; a core complex of 26 proteins, termed Mediator, and a larger complex containing the CDK8 kinase module, termed CDK-Mediator. Interestingly, 3 subunits of the kinase module have undergone independent gene duplications in vertebrates to generate the paralog pairs MED12/MED12L, MED13/ MED13L, and CDK8/CDK19. Each has been shown to interact with Mediator, yet clearly defining the composition of CDK kinase module has been challenging due to the large size (~600 kD), the similarities between paralogs, and potential combinatorial nature of complexes.
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