Brain-Derived Neurotrophic Factor, and Neurotrophin 3 in the Rat
Proc. Nati. Acad. Sci. USA Vol. 88, pp. 10352-10356, November 1991 Neurobiology Expanded distribution of mRNA for nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 in the rat brain after colchicine treatment (neurotrophic factor/regulation/localization/in situ hybridization) S. CECCATELLI*t, P. ERNFORSt, M. J. VILLAR*§, H. PERSSONt, AND T. HOKFELT* Departments of *Histology and Neurobiology and of tMedical Chemistry, Laboratory of Molecular Neurobiology, Karolinska Institute, Stockholm, Sweden; and lnstituto de Neurobiologfa, Buenos Aires, Argentina Contributed by T. Hokfelt, August 16, 1991 ABSTRACT The effect of intracerebroventricular iqjec- motoneurons after axotomy (11) and after intracerebroven- tion of the mitosis inhibitor colchicine on expression of mRNA tricular injection of colchicine (12). for nerve growth factor (NGF), brain-derived neurotrophic During the last years much interest has been focused on factor (BDNF), and neurotrophin 3 was studied in the rat brain NGF (13), the first member of a family of structurally related with in situ hybridization. Colchicine up-regulates mRNA for neurotrophic factors including brain-derived neurotrophic NGF and BDNF in many of the neuronal systems normally factor (BDNF) (14, 15), neurotrophin 3 (NT3; also called expressing these factors. In addition, after colchicine treatment hippocampus-derived neurotrophic factor) (16-20), and neu- NGF and BDNF mRNAs were localized in several brain areas rotrophin 4 (21). NGF, BDNF, and NT3 are all expressed in where they normally
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