DOCSLIB.ORG

The Invasive Snail Pseudosuccinea Columella

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Invasive Snail Pseudosuccinea Columella Invasion Biology Meets Parasitology: A Case Study of Parasite Spill-Back with Egyptian Fasciola gigantica in the Invasive Snail Pseudosuccinea columella Daniel S. Grabner1*, Faten A. M. M. Mohamed2, Milen Nachev1, Eman M. H. Me´abed2, Abdel Hameed A. Sabry2, Bernd Sures1 1 Aquatic Ecology and Centre for Water and Environmental Research, University of Duisburg-Essen, Essen, Germany, 2 Parasitology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt Abstract The liver fluke Fasciola gigantica is a trematode parasite of ruminants and humans that occurs naturally in Africa and Asia. Cases of human fascioliasis, attributable at least in part to F. gigantica, are significantly increasing in the last decades. The introduced snail species Galba truncatula was already identified to be an important intermediate host for this parasite and the efficient invader Pseudosuccinea columella is another suspect in this case. Therefore, we investigated snails collected in irrigation canals in Fayoum governorate in Egypt for prevalence of trematodes with focus on P. columella and its role for the transmission of F. gigantica. Species were identified morphologically and by partial sequencing of the cytochrome oxidase subunit I gene (COI). Among all 689 snails found at the 21 sampling sites, P. columella was the most abundant snail with 296 individuals (42.96%) and it was also the most dominant species at 10 sites. It was not found at 8 sites. Molecular detection by PCR and sequencing of the ITS1-5.8S-ITS2 region of the ribosomal DNA (rDNA) revealed infections with F. gigantica (3.38%), Echinostoma caproni (2.36%) and another echinostome (7.09%) that could not be identified further according to its sequence. No dependency of snail size and trematode infection was found. Both high abundance of P. columella in the Fayoum irrigation system and common infection with F. gigantica might be a case of parasite spill-back (increased prevalence in local final hosts due to highly susceptible introduced intermediate host species) from the introduced P. columella to the human population, explaining at least partly the observed increase of reported fascioliasis-cases in Egypt. Eichhornia crassipes, the invasive water hyacinth, which covers huge areas of the irrigation canals, offers safe refuges for the amphibious P. columella during molluscicide application. As a consequence, this snail dominates snail communities and efficiently transmits F. gigantica. Citation: Grabner DS, Mohamed FAMM, Nachev M, Me´abed EMH, Sabry AHA, et al. (2014) Invasion Biology Meets Parasitology: A Case Study of Parasite Spill-Back with Egyptian Fasciola gigantica in the Invasive Snail Pseudosuccinea columella. PLoS ONE 9(2): e88537. doi:10.1371/journal.pone.0088537 Editor: Shoba Ranganathan, Macquarie University, Australia Received October 22, 2013; Accepted January 6, 2014; Published February 11, 2014 Copyright: ß 2014 Grabner et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This study was conducted in the framework of the Integrated Water Technologies (IWaTec) joint project between the Centre for Water and Environmental Research (ZWU) at the University of Duisburg-Essen and the Fayoum University in Egypt. IWaTec is funded by the German Academic Exchange Service (DAAD) and financed by the Federal Foreign Office. This publication has been supported by the Open Access program of the German Research Foundation (Deutsche Forschungsgemeinschaft). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] Introduction technique in Fayoum area. Especially in Egypt, fascioliasis is an increasing problem, reaching prevalences in animals of sometimes The liver fluke Fasciola gigantica is a trematode parasite native to more than 50% and up to 19% in humans [5–7]. According to Africa and Asia and infects ruminants, but also humans as final estimations of the World Health Organization [8], at least 830,000 hosts. Adult F. gigantica live in the bile ducts of the liver, where they people are infected with either the introduced Fasciola hepatica or F. can reach a length of up to 76 mm. Infection with this parasite can gigantica in the Nile delta. Presence of both of these closely related cause severe disease symptoms referred to as fascioliasis [1]. species has been confirmed for Egypt [1,9,10], but they are usually Intermediate hosts are various lymnaeid snails [2], inside of which not distinguished diagnostically [11]. the parasite proliferates asexually and produces free swimming The natural first intermediate host of F. gigantica in Egypt is the cercariae that will attach to submerged surfaces, mostly plants. snail Radix natalensis, but the trematode was also found commonly These cercariae develop into encysted and durable metacercariae in the introduced species Galba truncatula [12]. Additionally, single that are transmitted when the final host ingests the metacercariae cases of F. gigantica infections were reported from Biomphalaria together with plants, or by consumption of water contaminated alexandrina and Pseudosuccinea columella in Egypt [13–15]. For the with metacercariae [3]. Due to the durability of the metacercariae, latter species, release of F. gigantica cercariae was proven in transmission can also be mediated by ingestion of terrestrial plants laboratory infections [16]. The highly invasive lymnaeid snail P. and crops that were submerged in water containing infected snails columella was introduced from North America to many countries for a couple of weeks [1,4], which is a common irrigation worldwide and was reported from Africa for the first time in the PLOS ONE | www.plosone.org 1 February 2014 | Volume 9 | Issue 2 | e88537 Fasciola gigantica in Pseudosuccinea columella middle of the 20th century, where it is now widely distributed [17– ITS1-segment of both F. gigantica and F. hepatica, to distinguish 20]. P. columella is well known as a suitable host for F. hepatica [12], Fasciola spp. from host and infections with other trematodes. but its importance for the maintenance of the natural life cycle of Sequences and additional information for primers designed for the F. gigantica and the transmission to humans is uncertain. present study are given in table 1. One 20 ml PCR reaction mix According to the parasite spill back hypothesis, invasive species contained 4 mlof56Crimson Taq buffer (New England Biolabs), are colonized by local parasites. If the invader becomes abundant 0.2 mM dNTP mix (New England Biolabs), 0.5 mM of each and the parasite can develop successfully, a high number of primer 0.5 U Crimson Taq (New England Biolabs) and 1 ml transmitting stages will develop and increase local parasite template DNA. The mix was topped up to 20 ml with PCR grade abundance and prevalence [21]. In this way, free living invasive water. The DNA was amplified by a Labcycler (SensoQuest) species may help native parasites to increase their population size under the following conditions: initial denaturation at 95uC for and extend their distribution range [22]. P. columella is a very 5 min, 40 cycles of 95uC for 30 s, annealing (temperatures see efficient invader and became an important snail host for F. hepatica table 1) for 30 s and elongation at 72uC for 30 s followed by a final in many countries [12]. We hypothesize that this might be the case elongation of 72uC for 5 min. for the P. columella/F. gigantica–system as well. As a result, infected Sensitivity of the Trem2 F/Trem1 R primer pair was lower and P. columella might be responsible for the observed increase in not all samples were amplified successfully with the Crimson Taq, infection intensity and prevalence in livestock and in the human although tested positive before with Trem1 F/Trem1 R primers. population in Egypt. Therefore, we investigated the trematode Therefore, the more robust Phire Animal Tissue Direct PCR Kit species occurring in the invasive snail P. columella, collected from (Thermo Scientific) was used for amplification with those primers. irrigation channels in the Fayoum governorate where cases of Reactions contained 10 mlof26 Phire PCR Buffer, 0.5 mMof fascioliasis are commonly reported [23,24]. We assessed the each primer, 0.4 ml Phire Hot Start II DNA Polymerase and 1 ml potential of this invasive species as a host for trematodes, especially DNA. Water was added to 20 ml. PCR conditions were 98uC for for Fasciola spp. to estimate the effect of P. columella for the spread 5 min, 40 cycles of 98uC for 10 s, annealing at 54uC for 10 s, of fascioliasis in the area and found evidence for a possible spill- elongation at 72uC for 20 s and a final elongation at 72uC for back effect on animals and the human population. 1 min. PCR conditions for the LCO1490/HC02198 primers were as described in Folmer et al. [25]. PCR products were checked by Materials and Methods standard agarose gel electrophoresis, purified with a JETQUICK PCR Product Purification Spin Kit (Genomed) according to Snail sampling manufacturer’s instructions and sent for sequencing (GATC). Snails were collected from July to September 2012, 2 month Sequences were checked for homology with database entries by after the last molluscicide treatment, from water plants (mainly BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi). water hyacinths Eichhornia crassipes) and with dip nets in irrigation channels at 21 different sites in Fayoum governorate (surrounding Statistics Markaz El-Fayoum, Itsa, and Ibshway cities), Egypt. Samples were Mean prevalences and confidence intervals of parasite infections taken with permission of the local farmers owning the land were calculated with the program Quantitative Parasitology v.3.0 adjacent to the irrigation channels. No endangered or protected [26].
Load more

Recommended publications
  • Pseudosuccinea Columella: Age Resistance to Calicophoron Daubneyi Infection in Two Snail Populations
    Parasite 2015, 22,6 Ó Y. Dar et al., published by EDP Sciences, 2015 DOI: 10.1051/parasite/2015003 Available online at: www.parasite-journal.org RESEARCH ARTICLE OPEN ACCESS Pseudosuccinea columella: age resistance to Calicophoron daubneyi infection in two snail populations Yasser Dar1,2, Daniel Rondelaud2, Philippe Vignoles2, and Gilles Dreyfuss2,* 1 Department of Zoology, Faculty of Science, University of Tanta, Tanta, Egypt 2 INSERM 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France Received 26 November 2014, Accepted 21 January 2015, Published online 10 February 2015 Abstract – Individual infections of Egyptian and French Pseudosuccinea columella with five miracidia of Calicoph- oron daubneyi were carried out to determine whether this lymnaeid was capable of sustaining larval development of this parasite. On day 42 post-exposure (at 23 °C), infected snails were only noted in groups of individuals measuring 1 or 2 mm in height at miracidial exposure. Snail survival in the 2-mm groups was significantly higher than that noted in the 1-mm snails, whatever the geographic origin of snail population. In contrast, prevalence of C. daubneyi infec- tion was significantly greater in the 1-mm groups (15–20% versus 3.4–4.0% in the 2-mm snails). Low values were noted for the mean shell growth of infected snails at their death (3.1–4.0 mm) and the mean number of cercariae (<9 in the 1-mm groups, <19 in the 2-mm snails). No significant differences between snail populations and snails groups were noted for these last two parameters. Most infected snails died after a single cercarial shedding wave.
    [Show full text]
  • Molecular Characterization of Liver Fluke Intermediate Host Lymnaeids
    Veterinary Parasitology: Regional Studies and Reports 17 (2019) 100318 Contents lists available at ScienceDirect Veterinary Parasitology: Regional Studies and Reports journal homepage: www.elsevier.com/locate/vprsr Original Article Molecular characterization of liver fluke intermediate host lymnaeids (Gastropoda: Pulmonata) snails from selected regions of Okavango Delta of T Botswana, KwaZulu-Natal and Mpumalanga provinces of South Africa ⁎ Mokgadi P. Malatji , Jennifer Lamb, Samson Mukaratirwa School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville Campus, Durban 4001, South Africa ARTICLE INFO ABSTRACT Keywords: Lymnaeidae snail species are known to be intermediate hosts of human and livestock helminths parasites, Lymnaeidae especially Fasciola species. Identification of these species and their geographical distribution is important to ITS-2 better understand the epidemiology of the disease. Significant diversity has been observed in the shell mor- Okavango delta (OKD) phology of snails from the Lymnaeidae family and the systematics within this family is still unclear, especially KwaZulu-Natal (KZN) province when the anatomical traits among various species have been found to be homogeneous. Although there are Mpumalanga province records of lymnaeid species of southern Africa based on shell morphology and controversial anatomical traits, there is paucity of information on the molecular identification and phylogenetic relationships of the different taxa. Therefore, this study aimed at identifying populations of Lymnaeidae snails from selected sites of the Okavango Delta (OKD) in Botswana, and sites located in the KwaZulu-Natal (KZN) and Mpumalanga (MP) provinces of South Africa using molecular techniques. Lymnaeidae snails were collected from 8 locations from the Okavango delta in Botswana, 9 from KZN and one from MP provinces and were identified based on phy- logenetic analysis of the internal transcribed spacer (ITS-2).
    [Show full text]
  • Downloaded from Genbank
    Bargues et al. Parasites Vectors (2020) 13:171 https://doi.org/10.1186/s13071-020-04045-x Parasites & Vectors RESEARCH Open Access Genetic uniformity, geographical spread and anthropogenic habitat modifcations of lymnaeid vectors found in a One Health initiative in the highest human fascioliasis hyperendemic of the Bolivian Altiplano M. Dolores Bargues1*, Patricio Artigas1, Rene Angles2, David Osca1, Pamela Duran1, Paola Buchon3, R. Karina Gonzales‑Pomar3, Julio Pinto‑Mendieta3 and Santiago Mas‑Coma1 Abstract Background: Fascioliasis is a snail‑borne zoonotic trematodiasis emerging due to climate changes, anthropogenic environment modifcations, and livestock movements. Many areas where Fasciola hepatica is endemic in humans have been described in Latin America altitude areas. Highest prevalences and intensities were reported from four provinces of the northern Bolivian Altiplano, where preventive chemotherapy is ongoing. New strategies are now incorporated to decrease infection/re‑infection risk, assessment of human infection sources to enable efcient prevention measures, and additionally a One Health initiative in a selected zone. Subsequent extension of these pilot interventions to the remaining Altiplano is key. Methods: To verify reproducibility throughout, 133 specimens from 25 lymnaeid populations representative of the whole Altiplano, and 11 used for population dynamics studies, were analyzed by rDNA ITS2 and ITS1 and mtDNA cox1 and 16S sequencing to assess their classifcation, variability and geographical spread. Results: Lymnaeid populations proved to belong to a monomorphic group, Galba truncatula. Only a single cox1 mutation was found in a local population. Two cox1 haplotypes were new. Comparisons of transmission foci data from the 1990’s with those of 2018 demonstrated an endemic area expansion.
    [Show full text]
  • Mitochondrial Genome of Bulinus Truncatus (Gastropoda: Lymnaeoidea): Implications for Snail Systematics and Schistosome Epidemiology
    Journal Pre-proof Mitochondrial genome of Bulinus truncatus (Gastropoda: Lymnaeoidea): implications for snail systematics and schistosome epidemiology Neil D. Young, Liina Kinkar, Andreas J. Stroehlein, Pasi K. Korhonen, J. Russell Stothard, David Rollinson, Robin B. Gasser PII: S2667-114X(21)00011-X DOI: https://doi.org/10.1016/j.crpvbd.2021.100017 Reference: CRPVBD 100017 To appear in: Current Research in Parasitology and Vector-Borne Diseases Received Date: 21 January 2021 Revised Date: 10 February 2021 Accepted Date: 11 February 2021 Please cite this article as: Young ND, Kinkar L, Stroehlein AJ, Korhonen PK, Stothard JR, Rollinson D, Gasser RB, Mitochondrial genome of Bulinus truncatus (Gastropoda: Lymnaeoidea): implications for snail systematics and schistosome epidemiology, CORTEX, https://doi.org/10.1016/ j.crpvbd.2021.100017. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2021 The Author(s). Published by Elsevier B.V. Journal Pre-proof Mitochondrial genome of Bulinus truncatus (Gastropoda: Lymnaeoidea): implications for snail systematics and schistosome epidemiology Neil D. Young a,* , Liina Kinkar a, Andreas J. Stroehlein a, Pasi K. Korhonen a, J.
    [Show full text]
  • Detection of Galba Truncatula, Fasciola Hepatica And
    Aberystwyth University Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control? Jones, Rhys; Brophy, Peter; Davis, Chelsea; Davies, Teresa; Emberson, Holly; Rees Stevens, Pauline; Williams, Hefin Published in: Parasites & Vectors DOI: 10.1186/s13071-018-2928-z Publication date: 2018 Citation for published version (APA): Jones, R., Brophy, P., Davis, C., Davies, T., Emberson, H., Rees Stevens, P., & Williams, H. (2018). Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control? Parasites & Vectors, 11(N/A), [342]. https://doi.org/10.1186/s13071- 018-2928-z Document License CC BY General rights Copyright and moral rights for the publications made accessible in the Aberystwyth Research Portal (the Institutional Repository) are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the Aberystwyth Research Portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the Aberystwyth Research Portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. tel: +44 1970 62 2400 email: [email protected] Download date: 09.
    [Show full text]
  • Ribosomal DNA ITS–1 Sequencing of Galba Truncatula (Gastropoda, Lymnaeidae) and Its Potential Impact on Fascioliasis Transmission in Mendoza, Argentina M
    Animal Biodiversity and Conservation 29.2 (2006) 191 Ribosomal DNA ITS–1 sequencing of Galba truncatula (Gastropoda, Lymnaeidae) and its potential impact on fascioliasis transmission in Mendoza, Argentina M. D. Bargues, R. L. Mera y Sierra, H. G. Gómez, P. Artigas & S. Mas–Coma Bargues, M. D., Mera y Sierra, R. L., Gómez, H. G., Artigas P. & Mas–Coma, S., 2006. Ribosomal DNA ITS–1 sequencing of Galba truncatula (Gastropoda, Lymnaeidae) and its potential impact on fascioliasis transmission in Mendoza, Argentina. Animal Biodiversity and Conservation, 29.2: 191–194. Abstract Ribosomal DNA ITS–1 sequencing of Galba truncatula (Gastropoda, Lymnaeidae) and its potential impact on fascioliasis transmission in Mendoza, Argentina.— Sequencing of the rDNA ITS–1 proved that the lymnaeid snail species Galba truncatula is present in Argentina and that it belongs to the haplotype HC, the same as that responsible for the fascioliasis transmission in the human hyperendemic area with the highest human prevalences and intensities known, the Northern Bolivian Altiplano. Key words: Galba truncatula, Lymnaeid vectors, Human and animal fascioliasis, Transmission, Mendoza, Argentina. Resumen Secuenciación del ITS–1 del ADN ribosomal de Galba truncatula (Gastropoda, Lymnaeidae) y su impacto potencial en la transmisión de la fascioliasis en Mendoza, Argentina.— La secuenciación del ITS–1 del ADNr demostró que la especie de gasterópodo lymnaeido Galba truncatula se encuentra en Argentina y que pertenece al haplotipo HC, el mismo responsable de la transmisión de la fascioliasis en el área de hiperendemia humana con las mayores prevalencias e intensidades de fascioliasis conocidas, el Altiplano Norte Boliviano. Palabras clave: Galba truncatula, Vectores Lymnaeidae, Fascioliasis humana y animal, Transmisión, Mendoza, Argentina.
    [Show full text]
  • Clonal Amplification of Fasciola Hepatica in Galba Truncatula: Within and Between Isolate Variation of Triclabendazole- Susceptible and -Resistant Clones Jane E
    Hodgkinson et al. Parasites & Vectors (2018) 11:363 https://doi.org/10.1186/s13071-018-2952-z RESEARCH Open Access Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole- susceptible and -resistant clones Jane E. Hodgkinson1* , Krystyna Cwiklinski1,2, Nicola Beesley1, Catherine Hartley1, Katherine Allen1 and Diana J. L. Williams1 Abstract Background: Fasciola hepatica is of worldwide significance, impacting on the health, welfare and productivity of livestock and regarded by WHO as a re-emerging zoonosis. Triclabendazole (TCBZ), the drug of choice for controlling acute fasciolosis in livestock, is also the drug used to treat human infections. However TCBZ-resistance is now considered a major threat to the effective control of F. hepatica. It has yet to be demonstrated whether F. hepatica undergoes a genetic clonal expansion in the snail intermediate host, Galba truncatula,andtowhat extent amplification of genotypes within the snail facilitates accumulation of drug resistant parasites. Little is known about genotypic and phenotypic variation within and between F. hepatica isolates. Results: Six clonal isolates of F. hepatica (3× triclabendazole-resistant, TCBZ-R and 3× triclabendazole-susceptible, TCBZ-S) were generated. Snails infected with one miracidium started to shed cercariae 42–56 days post-infection and shed repeatedly up to a maximum of 11 times. A maximum of 884 cercariae were shed by one clonally-infected snail (FhLivS1) at a single time point, with > 3000 clonal metacercariae shed over its lifetime. Following experimental infection all 12 sheep were FEC positive at the time of TCBZ treatment. Sheep infected with one of three putative TCBZ-S clones and treated with TCBZ had no parasites in the liver at post-mortem, whilst sheep each infected with putative TCBZ-R isolates had 35–165 adult fluke at post-mortem, despite TCBZ treatment.
    [Show full text]
  • The Freshwater Snails (Mollusca: Gastropoda) of Mexico: Updated Checklist, Endemicity Hotspots, Threats and Conservation Status
    Revista Mexicana de Biodiversidad Revista Mexicana de Biodiversidad 91 (2020): e912909 Taxonomy and systematics The freshwater snails (Mollusca: Gastropoda) of Mexico: updated checklist, endemicity hotspots, threats and conservation status Los caracoles dulceacuícolas (Mollusca: Gastropoda) de México: listado actualizado, hotspots de endemicidad, amenazas y estado de conservación Alexander Czaja a, *, Iris Gabriela Meza-Sánchez a, José Luis Estrada-Rodríguez a, Ulises Romero-Méndez a, Jorge Sáenz-Mata a, Verónica Ávila-Rodríguez a, Jorge Luis Becerra-López a, Josué Raymundo Estrada-Arellano a, Gabriel Fernando Cardoza-Martínez a, David Ramiro Aguillón-Gutiérrez a, Diana Gabriela Cordero-Torres a, Alan P. Covich b a Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av.Universidad s/n, Fraccionamiento Filadelfia, 35010 Gómez Palacio, Durango, Mexico b Institute of Ecology, Odum School of Ecology, University of Georgia, 140 East Green Street, Athens, GA 30602-2202, USA *Corresponding author: [email protected] (A. Czaja) Received: 14 April 2019; accepted: 6 November 2019 Abstract We present an updated checklist of native Mexican freshwater gastropods with data on their general distribution, hotspots of endemicity, threats, and for the first time, their estimated conservation status. The list contains 193 species, representing 13 families and 61 genera. Of these, 103 species (53.4%) and 12 genera are endemic to Mexico, and 75 species are considered local endemics because of their restricted distribution to very small areas. Using NatureServe Ranking, 9 species (4.7%) are considered possibly or presumably extinct, 40 (20.7%) are critically imperiled, 30 (15.5%) are imperiled, 15 (7.8%) are vulnerable and only 64 (33.2%) are currently stable.
    [Show full text]
  • Malacofauna En Dos Sistemas Silvopastoriles En Estelí, Nicaragua
    Malacofauna en dos sistemas silvopastoriles en Estelí, Nicaragua Malacofauna in two silvopastoral systems in Estelí, Nicaragua González-Valdivia Noel Antonio1, Martínez-Puc Jesús Froylán1*, Echavarría-Góngora Elías de Jesus2 Datos del Artículo Resumen 1Departamento de Ingenierías, Instituto Tecnológico de Chiná, Tecnológico La diversidad malacológica en dos sistemas silvopastoriles tradicionales, uno en bosque seco (Las Mesitas) y otro en Nacional de México. Calle 8, entre 22 y 28, Chiná, Campeche, México. CP. bosque de pino-encino (Picacho-Tomabú), ubicados en el municipio de Estelí, Nicaragua fue determinada con el fin de 25420. establecer comparaciones entre los ensambles de moluscos terrestres entre ambos sistemas. Para esto, durante la estación [email protected] 2Departamento de Ciencias Económico lluviosa (mayo a octubre) se realizaron muestreos una vez establecidas las lluvias (agosto a septiembre), siguiendo el Administrativas, Instituto Tecnológico de 2 Chiná, Tecnológico Nacional de México. método de conteo directo en 20 parcelas de 2.25 m y 20 árboles seleccionados al azar para cada localidad. Los moluscos Calle 8, entre 22 y 28, Chiná, Campeche, México. CP. 25420. extraídos de las muestras fueron identificados en el Centro Malacológico de la Universidad Centro Americana (UCACM), en Managua. Se estimaron los índices de riqueza, diversidad, equidad, dominancia y abundancia de especies *Dirección de contacto: para cada sistema y la asociación existente entre estos. Se determinó la presencia de un total de 47 especies, 41 de ellas Departamento de Ingenierías, Instituto Tecnológico de Chiná, Tecnológico presentes en Las Mesitas y 22 en el Picacho-Tomabú, incluyendo las 16 especies comunes entre ambas localidades Nacional de México. Calle 8, entre 22 y 28, Chiná, Campeche, México.
    [Show full text]
  • Loads of Trematodes: Discovering Hidden Diversity of Paramphistomoids in Kenyan Ruminants
    131 Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants MARTINA R. LAIDEMITT1*, EVA T. ZAWADZKI1, SARA V. BRANT1, MARTIN W. MUTUKU2, GERALD M. MKOJI2 and ERIC S. LOKER1 1 Department of Biology, Center for Evolutionary and Theoretical Immunology, Parasite Division Museum of Southwestern Biology, University of New Mexico, 167 Castetter MSCO3 2020 Albuquerque, New Mexico 87131, USA 2 Center for Biotechnology Research and Development, Kenya Medical Research Institute (KEMRI), P.O. Box 54840- 00200, Nairobi, Kenya (Received 23 May 2016; revised 24 August 2016; accepted 7 September 2016; first published online 20 October 2016) SUMMARY Paramphistomoids are ubiquitous and widespread digeneans that infect a diverse range of definitive hosts, being particu- larly speciose in ruminants. We collected adult worms from cattle, goats and sheep from slaughterhouses, and cercariae from freshwater snails from ten localities in Central and West Kenya. We sequenced cox1 (690 bp) and internal transcribed region 2 (ITS2) (385 bp) genes from a small piece of 79 different adult worms and stained and mounted the remaining worm bodies for comparisons with available descriptions. We also sequenced cox1 and ITS2 from 41 cercariae/rediae samples collected from four different genera of planorbid snails. Combining morphological observations, host use infor- mation, genetic distance values and phylogenetic methods, we delineated 16 distinct clades of paramphistomoids. For four of the 16 clades, sequences from adult worms and cercariae/rediae matched, providing an independent assessment for their life cycles. Much work is yet to be done to resolve fully the relationships among paramphistomoids, but some correspond- ence between sequence- and anatomically based classifications were noted.
    [Show full text]
  • First Report of Larval Stages of Fasciola Hepatica in a Wild Population of Pseudosuccinea Columella from Cuba and the Caribbean
    First report of larval stages of Fasciola hepatica in a wild population of Pseudosuccinea columella from Cuba and the Caribbean. Alain Gutierrez, A. A. Vázquez, Y. Hevia, J. Sánchez, A. C. Correa, S. Hurtrez-Boussès, J.-P. Pointier, A. Théron To cite this version: Alain Gutierrez, A. A. Vázquez, Y. Hevia, J. Sánchez, A. C. Correa, et al.. First report of larval stages of Fasciola hepatica in a wild population of Pseudosuccinea columella from Cuba and the Caribbean.. Journal of Helminthology, Cambridge University Press (CUP), 2011, 85 (1), pp.109-11. 10.1017/S0022149X10000350. halsde-00608583 HAL Id: halsde-00608583 https://hal.archives-ouvertes.fr/halsde-00608583 Submitted on 7 Sep 2012 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Journal of Helminthology (2011) 85, 109–111 doi:10.1017/S0022149X10000350 q Cambridge University Press 2010 First report of larval stages of Fasciola hepatica in a wild population of Pseudosuccinea columella from Cuba and the Caribbean A. Gutie´rrez1, A.A. Va´zquez1, Y. Hevia1,J.Sa´nchez1, A.C. Correa2, S. Hurtrez-Bousse`s3, J.-P. Pointier4 and A. The´ron5* 1Laboratorio de Malacologia, IPK, Apartado 601, Marianao 13, C.
    [Show full text]
  • Resistant Pseudosuccinea Columella Snails to Fasciola Hepatica (Trematoda) Infection in Cuba : Ecological, Molecular and Phenotypical Aspects Annia Alba Menendez
    Comparative biology of susceptible and naturally- resistant Pseudosuccinea columella snails to Fasciola hepatica (Trematoda) infection in Cuba : ecological, molecular and phenotypical aspects Annia Alba Menendez To cite this version: Annia Alba Menendez. Comparative biology of susceptible and naturally- resistant Pseudosuccinea columella snails to Fasciola hepatica (Trematoda) infection in Cuba : ecological, molecular and phe- notypical aspects. Parasitology. Université de Perpignan; Instituto Pedro Kouri (La Havane, Cuba), 2018. English. NNT : 2018PERP0055. tel-02133876 HAL Id: tel-02133876 https://tel.archives-ouvertes.fr/tel-02133876 Submitted on 20 May 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Délivré par UNIVERSITE DE PERPIGNAN VIA DOMITIA En co-tutelle avec Instituto “Pedro Kourí” de Medicina Tropical Préparée au sein de l’ED305 Energie Environnement Et des unités de recherche : IHPE UMR 5244 / Laboratorio de Malacología Spécialité : Biologie Présentée par Annia ALBA MENENDEZ Comparative biology of susceptible and naturally- resistant Pseudosuccinea columella snails to Fasciola hepatica (Trematoda) infection in Cuba: ecological, molecular and phenotypical aspects Soutenue le 12 décembre 2018 devant le jury composé de Mme. Christine COUSTAU, Rapporteur Directeur de Recherche CNRS, INRA Sophia Antipolis M. Philippe JARNE, Rapporteur Directeur de recherche CNRS, CEFE, Montpellier Mme.
    [Show full text]