Opakovatelnost a Personalita V Testech Exploračního Chování

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Opakovatelnost a Personalita V Testech Exploračního Chování Univerzita Karlova v Praze Přírodov ědecká fakulta Katedra zoologie Odd ělení ekologie a etologie Studijní program: Biologie Studijní obor: Zoologie Bc. Barbora Žampachová Opakovatelnost a personalita v testech explora čního chování Repeatability and personality in tests of exploratory behaviour DIPLOMOVÁ PRÁCE Školitel: Doc. RNDr. Daniel Frynta, PhD. Konzultant: RNDr. Eva Landová, PhD. Praha, 2015 Prohlášení: Prohlašuji, že jsem záv ěre čnou práci zpracovala samostatn ě a že jsem uvedla všechny použité informa ční zdroje a literaturu. Tato práce ani její podstatná část nebyla p ředložena k získání jiného nebo stejného akademického titulu. V Praze dne 13.8. 2015 …………………………… Bc. Barbora Žampachová Pod ěkování Na tomto míst ě bych ráda pod ěkovala p ředevším svému školiteli docentu Danielu Fryntovi a své konzultantce doktorce Ev ě Landové za zajímavé téma práce a neocenitelnou pomoc p ři statistickém vyhodnocení a rady p ři sepisování práce. Velké pod ěkování pat ří také Han ě Šimánkové a Barbo ře Kaftanové za rady a pomoc, bez kterých by tato práce nevznikla. Také bych cht ěla pod ěkovat svojí rodin ě za veškerou podporu, kterou mi p ři studiu poskytli. Abstrakt Personalita, tedy individuální rozdíly mezi zví řaty, stabilní v čase i mezi kontexty, je vysoce populárním tématem. V poslední dob ě se do pop ředí zájmu dostává v souvislosti s personalitou i tzv. opakovatelnost, která m ěř í práv ě stabilitu t ěchto rozdíl ů v čase. Cílem této diplomové práce je vyhodnotit personalitu krys v běžn ě používaných testech chování v novém prost ředí (open field test, hole board test) a srovnat, jak chování v těchto testech vzájemn ě koreluje a jak se m ění v čase. Každý test byl zopakován osmkrát, s různými intervaly (24 hodin, 6 dní, 4 týdny). Z výsledk ů lze odvodit, že nejvíce variability chování v těchto testech vysv ětlují t ři hlavní osy. První z nich souvisí s lokomotorickou aktivitou. Prvky chování, sytící tuto osu, jsou nejlépe opakovatelné. Druhá osa je sycená p řevážn ě časem, který zví řata stráví v centrální části arény, a třetí osa p ředstavuje zájem o díry v hole board testu. Tyto dv ě osy jsou opakovatelné h ůř e. U všech prvk ů chování, spojených s těmito osami, se ale objevil pr ůkazný efekt identity zví řete, který p ředstavuje personalitu. Klí čová slova: opakovatelnost, personalita, explorace, neofobie, open field test, hole board test Abstract Personality, or behavioural differences among individuals, which are stable both in time and across contexts, is a highly popular topic. Currently there has been an increase of interest in the relationship between personality and repeatability, which is a methodical approach developed to measure the stability of interindividual differences in time. The aim of this thesis is to evaluate the personality of rats according to behavioural patterns exhibited under widely used testing procedures in new environment (open field test, hole board test) and to compare, how behavioural traits in these tests mutually correlate and change over time. Each test trial was repeated eight times with different intervals (24 hours, 6 days, 4 weeks). The results suggest that most of the recorded behavioural variability can be explained with three principal axes. The first one is associated with loco-exploratory activity of the subject. The elements of behaviour associated with this axis are the most repeatable. The second axis is mostly associated with time the animal spent in the central part of the arena and the third axis represents the interest in holes in hole board test. These two axes are less repeatable. A significant effect of the identity of the animal was found in all behavioural traits, associated with these axes. Key words: repeatability, personality, exploration, neophobia, open field test, hole board test Obsah 1. Úvod ....................................................................................................................................... 1 2. Teoretický úvod .................................................................................................................... 2 2.1. Personalita ..................................................................................................................... 2 2.1.1 Aktivita ...................................................................................................................... 3 2.1.2. Agresivita a socialita ............................................................................................. 4 2.1.3 Explorace a boldness ............................................................................................ 4 2.1.4 Souvislost mezi osami personality ...................................................................... 6 2.1.5 Intraindividuální variabilita (IIV) ............................................................................ 6 2.2. Opakovatelnost ............................................................................................................. 7 2.2.1 Metody výpo čtu opakovatelnosti v behaviorálních studiích ............................ 7 2.2.2 Dosavadní poznatky o opakovatelnosti chování ............................................... 9 2.3. Krysa obecná ( Rattus rattus ) .................................................................................... 13 2.3.1 Taxonomické za řazení a fylogenetické postavení krysy obecné ................. 14 2.3.2 P ůvod a geografické rozší ření krysy ................................................................. 15 2.3.3. Biologie krysy ....................................................................................................... 17 2.4. Explorace a neofobie u hlodavc ů, zejména u rodu Rattus .................................. 19 2.4.1 Chování v novém prost ředí ................................................................................ 19 2.4.2. Reakce na nový p ředm ět ................................................................................... 22 2.4.3. Rozdíly mezi pohlavími ...................................................................................... 23 2.4.4. Shrnutí .................................................................................................................. 23 3. Metodika .............................................................................................................................. 25 3.1 Behaviorální testování ................................................................................................ 26 3.2 Vyhodnocení záznam ů chování ................................................................................ 27 3.3 Statistické vyhodnocení .............................................................................................. 29 4. Výsledky .............................................................................................................................. 31 4.1 Explora ční analýza ...................................................................................................... 31 4.2 Modely ........................................................................................................................... 39 4.3 Opakovatelnost ............................................................................................................ 43 4.4 Osy personality ............................................................................................................ 48 4.5 Vztah mezi personalitou a fyziologickým stavem ................................................... 51 4.6 Reakce na nový p ředm ět (Novel object test) .......................................................... 51 5. Diskuze ................................................................................................................................ 53 5.1 Lokomotorická a explora ční aktivita ......................................................................... 53 5.2 Sezení v centrální části .............................................................................................. 56 5.3 Head-dipping ................................................................................................................ 57 5.4 Reakce na nový p ředm ět ........................................................................................... 58 5.4 Rozdíly mezi pohlavími ............................................................................................... 59 6. Záv ěr .................................................................................................................................... 61 7. Seznam literatury ............................................................................................................... 62 8. P řílohy .................................................................................................................................. 73 1. Úvod V poslední dob ě je velmi populárním tématem personalita u zví řat. Jedna z definic personality říká, že se jedná o opakovatelné rozdíly v chování mezi jedinci, zejména v agresivit ě, socialit ě, aktivit ě, boldness a exploraci (Réale et al., 2010; Careau & Garland, 2012). Existuje mnoho komplexních review o personalit ě (Sih et al., 2004a, 2004b; Carere & Eens, 2005; Réale et al., 2010) a práce zabývající se opakovatelností (Bell et al., 2009), ale velmi málo studií se zabývá pr ůběhem chování, spojeného s personalitou, v čase a často testy tohoto chování neopakuje nebo má jen malý po čet opakování (Biro, 2012). Jednou z nej čast ěji testovaných os personality je explorace, tedy reakce na nový podn ět, která je velmi často m ěř ena jako chování v novém
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