bioRxiv preprint doi: https://doi.org/10.1101/2021.01.27.428502; this version posted January 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Discovery of an IKK2 Site that Allosterically Controls Its Activation Sonjiala Jackson Hotchkiss1, Maria Carmen Mulero1, Garrett J. Chan1,2, Tapan Biswas1, Smarajit Polley1,4, Christine Ohn1, Srihari Konduri, Dionicio Siegel1,3, Özlem Demir1,2, Rommie E. Amaro1,2, and Gourisankar Ghosh1,* 1 Department of Chemistry & Biochemistry; 2 Department of Pharmacology, 3 School of Pharmacy, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 4 Current address: The Bose Institute, Kolkata, India *Corresponding author: Gourisankar Ghosh
[email protected] Department of Chemistry & Biochemistry University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093 AUTHOR CONTRIBUTIONS: G.G., D.S. and R.E.A. designed research; S.J.H., M.C.M., G.J.C., S.P., C.O. and S.K. performed research; S.J.H., M.C.M., T.B. and O.D. analyzed data; and G.G. and T.B. wrote the paper. COMPETING INTEREST STATEMENT: The authors declare no conflict of interest. CLASSIFICATION: Biological Sciences - Biochemistry Key Words: IkB Kinase (IKK), NF-kB (NF-kB), Hydrogen deuterium exchange mass spectrometry (HDX), Kinase domain (KD), allostery, Nucleotide bioRxiv preprint doi: https://doi.org/10.1101/2021.01.27.428502; this version posted January 27, 2021.