Menks WM, Raschle NM. J Neurol Neuromedicine (2017) 2(7): 16-20 Neuromedicine www.jneurology.com www.jneurology.com Journal of Neurology & Neuromedicine Mini Review Open Access What can be learned from white matter alterations in antisocial girls Willeke M. Menks1, Christina Stadler1 and Nora M. Raschle1 1Department of Child and Adolescent Psychiatry, University of Basel, Psychiatric University Hospital Basel, Switzerland. Article Info ABSTRACT Article Notes Antisocial behavior in youths constitutes a major public health problem Received: June 17, 2017 worldwide. Conduct disorder is a severe variant of antisocial behavior with higher Accepted: July 31, 2017 prevalence rates for boys (12%) as opposed to girls (7%). A better understanding *Correspondence: of the underlying neurobiological mechanisms of conduct disorder is warranted Dr. Willeke Menks, PhD to improve identification, diagnosis, or treatment. Functional and structural Department of Child and Adolescent Psychiatry (KJPK), neuroimaging studies have indicated several key brain regions within the limbic Psychiatric University Clinics Basel (UPK) system and prefrontal cortex that are altered in youths with conduct disorder. Schanzenstrasse 13, CH-4056 Basel, Switzerland Examining the structural connectivity, i.e. white matter fiber tracts connecting Tel. +41 61 265 89 76 these brain areas, may further inform about the underlying neural mechanisms. Fax +41 61 265 89 61 Diffusion tensor imaging (DTI) is a non-invasive technique that can evaluate the © 2017 Menks WM & Raschle NM. This article is distributed white matter integrity of fiber tracts throughout the brain. To date, DTI studies have under the terms of the Creative Commons Attribution 4.0 found several white matter tracts that are altered in youths with conduct disorder. International License However, a majority of these studies have focused on male or mixed-gender groups, and only a few studies have specifically investigated white matter alterations in Keywords girls with conduct disorder. Ultimately, studies that directly compare boys and girls Antisocial behavior Conduct disorder with conduct disorder are necessary to identify possible sexual dimorphic neural Diffusion tensor imaging alterations and developmental trajectories of conduct disorder in youths. Pediatric neuroimaging Gender White brain matter Introduction Antisocial behavior in children and adolescents is associated with significant societal, clinical, and economic consequences and is therefore considered a major public health burden worldwide. Youths with severe aggressive and antisocial behavior outside of the age-appropriate norm qualify for a diagnosis of conduct disorder. Conduct disorder1 is characterized by a repetitive and persistent pattern of behavior in which the basic rights of others or societal norms2 or rules are violated . The life time prevalence of conduct disorder is estimated to be around 7% and 12%, for girls and boys respectively . Furthermore, conduct3 disorder is thought to be very heterogeneous and several subtypes have been suggested (e.g. with or without callous-unemotional traits ). Particularly youths with severe subtypes of conduct disorder are at higher risk for persistent antisocial behavior and criminality in adulthood, and thus 1,4at great risk for developing antisocial personality disorder later in life . Previous studies have suggested that an increased understanding of the neurobiological5 basis of conduct disorder and its subtypes in youths may increase the modest treatment success of current intervention methods . So far, research studies using functional and structural neuroimaging techniques have identified dysfunctions and structural alterations within a set of cortical and subcortical brain regions. Cortical brain regions that are often identified as being altered in conduct disorder by voxel-based approaches include gray matter structures of the limbic system (e.g. amygdala, insula, and cingulate cortex) and Page 16 of 20 Menks WM, Raschle NM. J Neurol Neuromedicine (2017) 2(7): 16-20 Journal of Neurology & Neuromedicine Diffusion Tensor Imaging 6-12 prefrontal cortex . The limbic system is important for various processes in human9 social behavior that are often A commonly used neuroimaging technique allowing the impaired in youths with conduct disorder, such as emotion investigation of the microstructural properties of white- processing and regulation . The prefrontal cortex (PFC) matter is diffusion tensor imaging (DTI). DTI is based plays a significant role13 in cognitive control by means of on the three-dimensional diffusion of water molecules attention and decision-making over the simple and more that is measured through multiple-directional diffusion- automatic behaviors . The amygdala and prefrontal cortex weighting gradient pulses. Hereby, specific features of this are bi-directionally interconnected through14 white matter diffusivity 22are translated into tensors using mathematical tracts of the prefrontal-limbic circuitry . Investigating the equations based on the eigenvectors and eigenvalues within functional and structural connectivity of this circuitry15-17 could each voxel . The most commonly used tensors to inform Braingive more connectivity insight in the in conductetiology ofdisorder conduct disorder . about microstructural integrity of white matter tracts are the fractional anisotropy (FA) and mean23 diffusivity (MD), which measure the anisotropic fraction of diffusivity and In line with individual reports, meta-analyses have the diffusion magnitude respectively . Other tensors summarized that brain regions commonly affected in occasionally used in DTI studies are axial diffusivity conduct disorder are part of specific neural networks, (magnitude of fastest diffusion direction) and radial24 specifically the18,19 emotion processing and regulation network diffusivity (diffusion magnitude of transverse25 direction) . (see figure 1) . Brain areas within those networks are In order to analyze group differences, researchers either functionally and structurally interconnected with one employ tract-based spatial statistics (TBSS) , voxel-based another by anatomical white matter tracts consisting analysis,Altered whiteor fiber matter tractography. structures in conduct disorder of abundant thin myelinated axons. Thus far, studies investigating functional brain connectivity in youths with severe antisocial behavior have found a reduced functional DTI has aided studies investigating white matter connectivity between the amygdala and two regions of structures in children and adolescents with conduct the prefrontal7 cortex, namely the ventromedial17,20 prefrontal disorder, however, many discrepancies exist between cortex and the orbitofrontal cortex . Consequently, the results of these studies. For example, DTI studies researchers have hypothesized that the observed reduced have identified increased21,26-28 fractional anisotropy 29,30in the amygdala–PFC connectivity is correlated with15,21 abnormal uncinate fasciculus and corpus callosum of white matter structures in these youths . Although children and adolescents with conduct problems, while functional networks help to identify the neural dynamics others observed decreased26,31 white matter integrity in the between brain areas, it cannot inform about the actual same white matter tracts , as well as in the corona structural architecture. Hence, investigating white matter radiata, superior longitudinal fasciculus, fronto-occipital31,32 tracts will be an important step toward understanding fasciculus, stria terminalis, and cerebellar peduncle . the dysfunctional neural interplay and connectivity that Most studies vary in regard to the precise tract or set of contribute to conduct disorder. alterations at all tracts identified,20,33,34 and some do not detect white matter . Thus far, there is no clear picture about the underlying tract-based phenotype of antisocial behavior to date. Similar discrepancies exist when following-up on the relationship of specific DTI tensors in relation to behavioral severity of conduct disorder, such as psychopathic traits, callous unemotional31,32,35-37 traits, negativeand amount orof noconduct correlations disorder35,37 at symptomsall29 . Some identify 31,32positive correlations , while others reported . These inconsistent findings regarding the direction and location of white mater alterations may result from differences in the analysis approaches used (e.g. TBSS, voxel-based analysis, or fiber tractography), small sample sizes, or group heterogeneity. Especially group heterogeneity is of importance when Figure 1. Schematic overview of brain regions commonly affected in investigating antisocial behavior. The inclusion of youths conduct disorder: amygdala, insula, dorsomedial prefrontal cortex can be defined based on different diagnostic criteria; some (dmPFC), and the orbitofrontal cortex (OFC). studies investigated only oppositional defiant disorder, while others focused on conduct disorder or a mixture of both. These heterogeneities were previously mentioned in Page 17 of 20 Menks WM, Raschle NM. J Neurol Neuromedicine (2017) 2(7): 16-20 Journal of Neurology & Neuromedicine a recent review38 , however, we suggest another important levelstudies have directly and indirectly investigated sex factor in antisocial behavior, namely sex. So far, most DTI differences28,29,50 in youths with conduct2 disorder on a neural studies have included only male or mixed-gender groups , which is likely caused by the lower prevalence-