RFC5 Antibody Cat. No.: 23-370

RFC5 Antibody

Immunohistochemistry of paraffin-embedded human lung cancer using RFC5 antibody (23-370) at dilution of 1:100 (40x lens).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion containing a sequence corresponding to amino acids 181-340 IMMUNOGEN: of human RFC5 (NP_031396.1).

TESTED APPLICATIONS: IHC, WB

WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200

POSITIVE CONTROL: 1) HeLa

2) U-251MG

September 26, 2021 1 https://www.prosci-inc.com/rfc5-antibody-23-370.html 3) SKOV3

4) MCF-7

5) Mouse liver

6) Mouse spleen

PREDICTED MOLECULAR Observed: 38kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: RFC5

ALTERNATE NAMES: RFC5, MGC1155, RFC36

GENE ID: 5985

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proliferating cell nuclear antigen (PCNA) and (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This encodes the 36 kD subunit. BACKGROUND: This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. results in multiple transcript variants. A related pseudogene has been identified on 9.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 26, 2021 2 https://www.prosci-inc.com/rfc5-antibody-23-370.html