RFC5 Antibody Cat. No.: 23-370 RFC5 Antibody Immunohistochemistry of paraffin-embedded human lung cancer using RFC5 antibody (23-370) at dilution of 1:100 (40x lens). Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 IMMUNOGEN: of human RFC5 (NP_031396.1). TESTED APPLICATIONS: IHC, WB WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200 POSITIVE CONTROL: 1) HeLa 2) U-251MG September 26, 2021 1 https://www.prosci-inc.com/rfc5-antibody-23-370.html 3) SKOV3 4) MCF-7 5) Mouse liver 6) Mouse spleen PREDICTED MOLECULAR Observed: 38kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: RFC5 ALTERNATE NAMES: RFC5, MGC1155, RFC36 GENE ID: 5985 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. BACKGROUND: This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 2 https://www.prosci-inc.com/rfc5-antibody-23-370.html.