Product Information

Griffithsin Item No. 10340 • Lot No. XXXX

Synonym: GRFT Source: Recombinant N-terminal hexahistdine-tagged GRFT expressed in E. coli Amino Acids: 2-122 of GRFT (out of 139 total aa in ) Accession No.: AMC42413 Mr: 14.6 kDa Purity: ≥95% Stability: ≥9 months at -80°C Supplied in: 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride, 2.5 mM DTT, and 20% glycerol Protein Concentration: batch specific mg/ml 1 2 3 4 Griffithsin (GRFT), is a lectin isolated from Griffithsia sp. with anti-HIV activity at subnanomolar concentrations.1 GRFT activity is glycosylation-dependent and acts by binding to glycoproteins , gp120, and gp160 on the viral envelope, blocking the virus from binding to CD4 receptor-expressing cells in the host. It also prevents cell fusion between infected and uninfected cells, further inhibiting the spread of HIV in the body. GRFT functions as a homodimer, with each monomer containing three carbohydrate binding sites.1,2 GRFT’s unique anti-viral activity is of interest for use in microbicide production to stop transmission of HIV through sexual contact. It has also been shown to have inhibitory effects with SARS-related ; where GRFT binding to the SARS-CoV S protein can prevent viral entry and reduce viral load in succeeding rounds of infection.3

References

1. Mori, T., O’Keefe, B.R., Sowder, R.C., II, et al. Isolation and characterization of Lane 1: MW Marker griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp. The Journal Lane 2: GRFT (5 µg) of Biological Chemistry 280(10) Lane 3: GRFT (2.5 µg) , 9345-9353 (2005). Lane 4: GRFT (1.25 µg) 2. Ziólkowska, N.E., O’Keefe, B.R., Mori, T., et al. Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding. Structure 14, 1127-1135 (2006). 3. O’Keefe, B.R., Giomarelli, B., Barnard, D.L., et al. Broad spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J. Virol. (2009).

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Cayman Chemical

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