Article Interaction of Biochar Type and Rhizobia Inoculation Increases the Growth and Biological Nitrogen Fixation of Robinia pseudoacacia Seedlings Qiaoyu Sun 1, Yong Liu 2, Hongbin Liu 1 and R. Kasten Dumroese 3,* 1 Key Laboratory of Non-Point Source Pollution Control, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 10081, China;
[email protected] (Q.S.);
[email protected] (H.L.) 2 Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing 100083, China;
[email protected] 3 US Department of Agriculture, Forest Service, Rocky Mountain Research Station, 1221 South Main Street, Moscow, ID 83843, USA * Correspondence:
[email protected]; Tel.: +1-208-883-2324; Fax: +1-208-882-3915 Received: 29 May 2020; Accepted: 23 June 2020; Published: 26 June 2020 Abstract: Adding biochar to soil can change soil properties and subsequently affect plant growth, but this effect can vary because of different feedstocks and methods (e.g., pyrolysis or gasification) used to create the biochar. Growth and biological nitrogen fixation (BNF) of leguminous plants can be improved with rhizobia inoculation that fosters nodule development. Thus, this factorial greenhouse study examined the effects of two types of biochar (i.e., pyrolysis and gasification) added at a rate of 5% (v:v) to a peat-based growth substrate and rhizobia inoculation (yes or no) on 15 15 Robinia pseudoacacia (black locust) seedlings supplied with NH4 NO3. Seedling and nodule growth, nitrogen (N) content, and δ15N 1000 were evaluated after 3 months.