DOCSLIB.ORG

Stem-Nodulating Nitrogen-Fixing Bacterium Isolated from Sesbania Rostrata

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Stem-Nodulating Nitrogen-Fixing Bacterium Isolated from Sesbania Rostrata INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1988, p. 89-98 Vol. 38, No. 1 0020-7713/88/010089-10$02 .OO/O Copyright 0 1988, International Union of Microbiological Societies Characterization of Azorhizobium caulinodans gen. nov. sp. nov., a Stem-Nodulating Nitrogen-Fixing Bacterium Isolated from Sesbania rostrata B. DREYFUS,1*2*J. L. GARCIA,3 AND M. GILLIS4 Laboratoire de Biologie des Sols, O.R.S.T.O.M.,B.P. 1386, Dakar, Senegal’; Laboratorium voor Genetica, Rijksuniversiteit, Ledeganckstraat 35, B-9000 Gent, Belgium2; Laboratoire de Microbiologie, O.R.S.T.O.M., Universite‘ de Provence, 13331 Marseille Cedex, France3; and Laboratorium voor Microbiologie en Microbiele Genetica, Ruksuniversiteit, B-9000 Gent, Belgium4 Twenty stem- and root-nodulating bacterial strains isolated from stem nodules of Sesbania rostrata were compared by numerical analysis of 221 phenotypic features with nine strains which effectively nodulate only the roots of this plant and with representative strains from the genera Rhizobium and Bradyrhizobium. Representative organisms from the different clusters were investigated further, together with possibly related organisms, by performing comparative gel electrophoresis of whole-cell proteins and by performing deoxyri- bonucleic acid (DNA)-DNA and DNA-ribosomal ribonucleic acid (rRNA) hybridizations. 3H-labeledrRNA was prepared from Sesbunia stem- and root-nodulating bacterial strain ORS 571T (T = type strain); [14C]rRNA from Bradyrhizobium japonicum NZP 5549T was also used. The following conclusions were drawn: (i) the Sesbania root-nodulating bacterial strains are genuine rhizobia; (ii) the Sesbania stem- and root-nodulating strains are quite different from Rhizobium and Bradyrhizobium, and thus they constitute a separate rRNA subbranch on the Rhodopseudomonas palusfris rRNA branch in rRNA superfamily IV; and (iii) the closest relative of these organisms is Xanthobacfer, but they are phenotypically and genotypically sufficiently different from the latter genus to deserve a separate generic rank. Because the feature of free-living nitrogen fixation is quite discriminative, a new genus, Azorhizobium, is proposed, with one species, Azorhizobium caulinodans. The type strain is strain ORS 571 (= LMG 6465). Bacteria which form nitrogen-fixing nodules on legumi- to belong to the Rhodopseudomonas palustris rRNA branch nous plants are currently divided into two genera, Rhizo- and consequently to be a relative of the latter taxon and of bium and Bradyrhizobium (17). The genus Rhizobium com- Bradyrhizobium, although it does not belong in either of prises four species, Rhizobium leguminosarum, Rhizobium these taxa. meliloti, Rhizobium loti, and Rhizobium fredii. The species To determine the exact taxonomic position of this new Rhizobium leguminosarum regroups three former species, group of N,-fixing , nodulating organisms, we analyzed a Rhizobium trifolii, Rhizobium phaseoli, and Rhizobium le- collection of root- and stem-nodulating strains from S. guminosarum. All species of the genus Rhizobium are fast- rostrata from different geographical areas and compared growing bacteria. The genus Bradyrhizobium comprises one them with Sesbania root-nodulating strains and strains of well-defined species, Bradyrhizobium japonicum, and in- Rhizobium and Bradyrhizobium. These investigations in- cludes all of the bacteria previously referred to as slow- cluded a numerical analysis of phenotypic features, compar- growing rhizobia. ative protein gel electrophoresis, and DNA-DNA and DNA- Nitrogen-fixing nodules are usually found on the roots of rRNA hybridizations. the host leguminous plants. Only some legume species The results of this work led to a proposal for a new genus belonging to the genera Neptunia, Aeschynomene, and Ses- for the S. rostrata root- and stem-nodulating bacteria, Azo- bania bear nodules on both roots and stems (9). Recently, rhizobium, which we describe here; this genus contains one Dreyfus et al. (8) found that the tropical legume Sesbania species, Azorhizobium caulinodans. Below we use the latter rostrata was actually associated with the two types of strains name. described below. (i) Some strains are similar to strain ORS 571T (T = type strain), which fixes atmospheric N2 in culture MATERIALS AND METHODS and grows at the expense of this fixed N,; they always Bacterial strains. The bacterial strains which we studied nodulate both roots and stems of S. rostrata (10, 13). They are listed in Table 1. The purity of the cultures was assured are usually cited in the literature as “the Sesbania stem- by repeated use of single-colony isolates, and the identities nodulating strains.” We did not find such N,-fixing strains in of the nodulating strains were checked by plant infection stem nodules from Neptunia and Aeschynomene (B. tests on their original host plants. A total of 21 stem- and Dreyfus, unpublished data). (ii) Some strains do not fix N, in root-nodulating strains (Azorhizobium) were isolated from culture and generally nodulate the roots of S. rostrata only. stem nodules of S. rostrata growing in different regions of Recent results based on deoxyribonucleic acid (DNA)- Senegal and Madagascar by using standard procedures (29). ribosomal ribonucleic acid (rRNA) hybridizations (16) in- Eleven root-nodulating strains (Rhizobium) of S. rostrata dicate that two representative Sesbania root-nodulating were originally isolated from root nodules of S. rostrata, strains belong to the genus Rhizobium. On the other hand, Sesbania pachycarpa, Sesbania cannabina, and Sesbania stem- and root-nodulating strain ORS 571T has been shown grandflora. Fourteen Rhizobium strains were isolated from nodules of Acacia sp. and Leucaena sp. All of these isolates * Corresponding author. (except strains ORS 590, ORS 609, and ORS 611) were 89 90 DREYFUS ET AL. INT. J. SYST.BACTERIOL. - TABLE 1. Strain designations, origins, and comments Strain(s)' Originb Received from'? Comments - Azorhizobium caulinodans ORS 571' S. rostrata, from Senegal Our isolate Nodulates roots and stems of (= LMG 6465T), ORS 553, ORS 555, S. rostrata ORS 556, ORS 557, ORS 559, ORS 560, ORS 561, ORS 562, ORS 563, ORS 564, ORS 565, ORS 567, ORS 569, ORS 570, ORS 574, ORS 576, and ORS 577 A zorhizobium caulinodans ORS 590 S. rostrata, from Senegal I. Ndoye Nodulates roots and stems of S. rostrata A.zorhizobium caulinodans ORS 591 and S. rostrata, from Madagascar R. Fettiarisson Nodulates roots and stems of ORS 592 S. rostrata hlhizobium sp. strain ORS 22 (= LMG S. rostrata, from Senegal Our isolate Nodulates only roots of 6463) S. rostrata Rhizobium sp. strain ORS 51 S. rostrata, from Senegal Our isolate Nodulates roots and forms (= LMG 6464) pseudonodules on stems of S. rostrata Rhizobium sp. strains ORS 52 and ORS 53 S. rostrata, from Senegal Our isolate Nodulates only roots of S. rostrata hthizobium sp. strain ORS 507 S. pachycarpa, from Senegal Our isolate Nodulates roots and forms pseudonodules on stems of S. rostrata hlhizobium sp. strains ORS 508, ORS 509, S. pachycarpa, from Senegal Our isolate Nodulates only roots of ORS 510, and ORS 511 S. rostrata Rhizobium sp. strain ORS 609 S. cannabina, from Senegal I. Ndoye Nodulates roots and stems of S. rostrata Rhizobium sp. strain ORS 611 S. grandifora, from Senegal Our isolate Nodulates roots and stems of S. rostrata hthizobium sp. strains ORS 901, ORS 902, Acacia senegal, from Senegal Our isolate ORS 903, ORS 906, and ORS 909 hlhizobium sp. strains ORS 904, ORS 905, Leucaena leucocephala, from Senegal Our isolate ORS 915, and ORS 916 h!hizobium sp. strain ORS 907 Acacia raddiana, from Senegal Our isolate hlhizobium sp. strains ORS 908 Acacia bivenosa , from Senegal Our isolate and ORS 910 Rhizobium sp. strains ORS 911 Acacia farnesiana, from Senegal Our isolate and ORS 912 Rhizobium meliloti B56, B57, and R29 Medicago sativa, from United States E. L. Schmidt Klhizobium meliloti R30 Medicago sativa, from Colombia CIAT h!hizobium meliloti LMG 6130 Medicago sativa, from New Zealand B. D. W. Jarvis (= NZP 4009) Klhizobium leguminosarum B62 and B63 Pisum sativum, from United States E. L. Schmidt Bradyrhizobium japonicum USDA 135 Glycine rnax, from United States USDA and USDA 138 Bradyrhizobium japonicum IITA 114, Glycine max, from Nigeria IITA IITA 101, and IITA 35 Bradyrhizobium japonicum LMG 6138' Glycine rnax, from New Zealand B. D. W. Jarvis (= NZP 5549') b!radyrhizobium sp. strains NZP 2314 Lotus pendiculatus, from New Zealand B. D. W. Jarvis and NZP 2309 Bradyrhizobium sp. strain ORS 801 Acacia holosericea, from Senegal Our isolate Bradyrhizobium sp. strain ORS 802 Acacia sieberiana, from Senegal Our isolate Bradyrhizobium sp. strain ORS 803 Acacia bivenosa, from Senegal Our isolate Bradyrhizobium sp. strain ORS 806 Leucaena leucocephala, from Senegal Our isolate Bradyrhizobium sp. strain ORS 809 Acacia albida, from Senegal Our isolate Bradyrhizobium sp. strains ORS 401, ORS Glycine max cv. Malayan, from Senegal Our isolate 403, and ORS 407 Bradyrhizobium sp. strain NIG 2 Glycine rnax cv. Malayan, from Senegal IITA Bradyrhizobium sp. strain GJ Glycine javanica, from Senegal Our isolate Bradyrhizobium sp. strain ORS 301 Aeschynomene afraspera, from Senegal Our isolate Bradyrhizobium sp. strain CB 756 Macrotylama africanum, from Zimbabwe CSIRO h!hodopseudomonas viridis DSM 135 DSM hlhodopseudomonas acidophila GD14 N. Pfennig hlhodopseudomonas acidophila ATCC ATCC 25092T Xanthobacter autotrophicus LMG 7043' DSM (= DSM 432') and LMG 7044 (= DSM 1393) Xanthobacterflavus LMG 7045' (= DSM DSM 338') B'eijerinckia
Load more

Recommended publications
  • Azorhizobium Doebereinerae Sp. Nov
    ARTICLE IN PRESS Systematic and Applied Microbiology 29 (2006) 197–206 www.elsevier.de/syapm Azorhizobium doebereinerae sp. Nov. Microsymbiont of Sesbania virgata (Caz.) Pers.$ Fa´tima Maria de Souza Moreiraa,Ã, Leonardo Cruzb,Se´rgio Miana de Fariac, Terence Marshd, Esperanza Martı´nez-Romeroe,Fa´bio de Oliveira Pedrosab, Rosa Maria Pitardc, J. Peter W. Youngf aDepto. Cieˆncia do solo, Universidade Federal de Lavras, C.P. 3037 , 37 200–000, Lavras, MG, Brazil bUniversidade Federal do Parana´, C.P. 19046, 81513-990, PR, Brazil cEmbrapa Agrobiologia, antiga estrada Rio, Sa˜o Paulo km 47, 23 851-970, Serope´dica, RJ, Brazil dCenter for Microbial Ecology, Michigan State University, MI 48824, USA eCentro de Investigacio´n sobre Fijacio´n de Nitro´geno, Universidad Nacional Auto´noma de Mexico, Apdo Postal 565-A, Cuernavaca, Mor, Me´xico fDepartment of Biology, University of York, PO Box 373, York YO10 5YW, UK Received 18 August 2005 Abstract Thirty-four rhizobium strains were isolated from root nodules of the fast-growing woody native species Sesbania virgata in different regions of southeast Brazil (Minas Gerais and Rio de Janeiro States). These isolates had cultural characteristics on YMA quite similar to Azorhizobium caulinodans (alkalinization, scant extracellular polysaccharide production, fast or intermediate growth rate). They exhibited a high similarity of phenotypic and genotypic characteristics among themselves and to a lesser extent with A. caulinodans. DNA:DNA hybridization and 16SrRNA sequences support their inclusion in the genus Azorhizobium, but not in the species A. caulinodans. The name A. doebereinerae is proposed, with isolate UFLA1-100 ( ¼ BR5401, ¼ LMG9993 ¼ SEMIA 6401) as the type strain.
    [Show full text]
  • Revised Taxonomy of the Family Rhizobiaceae, and Phylogeny of Mesorhizobia Nodulating Glycyrrhiza Spp
    Division of Microbiology and Biotechnology Department of Food and Environmental Sciences University of Helsinki Finland Revised taxonomy of the family Rhizobiaceae, and phylogeny of mesorhizobia nodulating Glycyrrhiza spp. Seyed Abdollah Mousavi Academic Dissertation To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public examination in lecture hall 3, Viikki building B, Latokartanonkaari 7, on the 20th of May 2016, at 12 o’clock noon. Helsinki 2016 Supervisor: Professor Kristina Lindström Department of Environmental Sciences University of Helsinki, Finland Pre-examiners: Professor Jaakko Hyvönen Department of Biosciences University of Helsinki, Finland Associate Professor Chang Fu Tian State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University, China Opponent: Professor J. Peter W. Young Department of Biology University of York, England Cover photo by Kristina Lindström Dissertationes Schola Doctoralis Scientiae Circumiectalis, Alimentariae, Biologicae ISSN 2342-5423 (print) ISSN 2342-5431 (online) ISBN 978-951-51-2111-0 (paperback) ISBN 978-951-51-2112-7 (PDF) Electronic version available at http://ethesis.helsinki.fi/ Unigrafia Helsinki 2016 2 ABSTRACT Studies of the taxonomy of bacteria were initiated in the last quarter of the 19th century when bacteria were classified in six genera placed in four tribes based on their morphological appearance. Since then the taxonomy of bacteria has been revolutionized several times. At present, 30 phyla belong to the domain “Bacteria”, which includes over 9600 species. Unlike many eukaryotes, bacteria lack complex morphological characters and practically phylogenetically informative fossils. It is partly due to these reasons that bacterial taxonomy is complicated.
    [Show full text]
  • 1 Horizontal Gene Transfer of a Unique Nif Island Drives Convergent Evolution of Free-Living
    bioRxiv preprint doi: https://doi.org/10.1101/2021.02.03.429501; this version posted February 3, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Horizontal gene transfer of a unique nif island drives convergent evolution of free-living 2 N2-fixing Bradyrhizobium 3 4 Jinjin Tao^, Sishuo Wang^, Tianhua Liao, Haiwei Luo* 5 6 Simon F. S. Li Marine Science Laboratory, School of Life Sciences and State Key Laboratory of 7 Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 8 9 ^These authors contribute equally to this work. 10 11 *Corresponding author: 12 Haiwei Luo 13 School of Life Sciences, The Chinese University of Hong Kong 14 Shatin, Hong Kong SAR 15 Phone: (+852) 39436121 16 E-mail: [email protected] 17 18 Running Title: Free-living Bradyrhizobium evolution 19 Keywords: free-living Bradyrhizobium, nitrogen fixation, lifestyle, HGT 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.03.429501; this version posted February 3, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 20 Summary 21 The alphaproteobacterial genus Bradyrhizobium has been best known as N2-fixing members that 22 nodulate legumes, supported by the nif and nod gene clusters.
    [Show full text]
  • DNA Homologies Among Members of the Genus Azorhizobium and Other Stem- and Root-Nodulating Bacteria Isolated from The
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1991, p. 114-120 Vol. 41, No. 1 0020-7713/91/010114-07$02.00/0 Copyright 0 1991, International Union of Microbiological Societies DNA Homologies among Members of the Genus Azorhizobium and Other Stem- and Root-Nodulating Bacteria Isolated from the I Tropical Legume Sesbania rostrata G. RINAUD0,172*S. ORENGA,3 M. P. FERNANDEZ,3 H. MEUGNIER,4 AND R. BARDIN3 Laboratoire d'Ecologie Microbienne, Universite' Claude Bernard Lyon I, F-69622 Villeurbanne Cedex,' Ofice de Recherche Scientifique et Technique Outre Mer2 and Unite' de Recherche Associe'e au Centre National de la Recherche Scienti$que 697,3 and Laboratoire de Bacte'riologie, Faculte' de Me'decine Alexis Carrel,4 F-69392 Lyon Cedex 8, France The diversity among 191 bacterial strains isolated from stem and root nodules (151 and 40 strains, respectively) of Sesbuniu rostrutu grown in different geographical areas in Senegal and in The Philippines was studied by using DNA-DNA hybridization techniques (S1 nuclease method), by determining DNA base compositions, by performing legume nodulation tests, and by determining nitrogenase activity. The following conclusions were drawn. (i) All of the strains produced stem and root nodules on S. rostrutu. (ii) Most of the organisms (184 strains) belonged to the genus Azorhizobium; their guanine-plus-cytosine contents ranged from 66 to 68 mol% ,they fixed N, under free-living conditions, and they produced effective nodules on the stems and roots of S. rostrata. (iii) The seven other strains probably belonged to the genus Rhizobium, since guanine- plus-cytosine contents ranged from 59 to 63 mol% and they did not fix N, under free-living conditions; three strains produced effective root nodules, but their stem nodules exhibited very low activity or were ineffective, and the four remaining strains produced ineffective nodules on both stems and roots.
    [Show full text]
  • Research Collection
    Research Collection Doctoral Thesis Development and application of molecular tools to investigate microbial alkaline phosphatase genes in soil Author(s): Ragot, Sabine A. Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010630685 Rights / License: In Copyright - Non-Commercial Use Permitted This page was generated automatically upon download from the ETH Zurich Research Collection. For more information please consult the Terms of use. ETH Library DISS. ETH NO.23284 DEVELOPMENT AND APPLICATION OF MOLECULAR TOOLS TO INVESTIGATE MICROBIAL ALKALINE PHOSPHATASE GENES IN SOIL A thesis submitted to attain the degree of DOCTOR OF SCIENCES of ETH ZURICH (Dr. sc. ETH Zurich) presented by SABINE ANNE RAGOT Master of Science UZH in Biology born on 25.02.1987 citizen of Fribourg, FR accepted on the recommendation of Prof. Dr. Emmanuel Frossard, examiner PD Dr. Else Katrin Bünemann-König, co-examiner Prof. Dr. Michael Kertesz, co-examiner Dr. Claude Plassard, co-examiner 2016 Sabine Anne Ragot: Development and application of molecular tools to investigate microbial alkaline phosphatase genes in soil, c 2016 ⃝ ABSTRACT Phosphatase enzymes play an important role in soil phosphorus cycling by hydrolyzing organic phosphorus to orthophosphate, which can be taken up by plants and microorgan- isms. PhoD and PhoX alkaline phosphatases and AcpA acid phosphatase are produced by microorganisms in response to phosphorus limitation in the environment. In this thesis, the current knowledge of the prevalence of phoD and phoX in the environment and of their taxonomic distribution was assessed, and new molecular tools were developed to target the phoD and phoX alkaline phosphatase genes in soil microorganisms.
    [Show full text]
  • 2010.-Hungria-MLI.Pdf
    Mohammad Saghir Khan l Almas Zaidi Javed Musarrat Editors Microbes for Legume Improvement SpringerWienNewYork Editors Dr. Mohammad Saghir Khan Dr. Almas Zaidi Aligarh Muslim University Aligarh Muslim University Fac. Agricultural Sciences Fac. Agricultural Sciences Dept. Agricultural Microbiology Dept. Agricultural Microbiology 202002 Aligarh 202002 Aligarh India India [email protected] [email protected] Prof. Dr. Javed Musarrat Aligarh Muslim University Fac. Agricultural Sciences Dept. Agricultural Microbiology 202002 Aligarh India [email protected] This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machines or similar means, and storage in data banks. Product Liability: The publisher can give no guarantee for all the information contained in this book. The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. # 2010 Springer-Verlag/Wien Printed in Germany SpringerWienNewYork is a part of Springer Science+Business Media springer.at Typesetting: SPI, Pondicherry, India Printed on acid-free and chlorine-free bleached paper SPIN: 12711161 With 23 (partly coloured) Figures Library of Congress Control Number: 2010931546 ISBN 978-3-211-99752-9 e-ISBN 978-3-211-99753-6 DOI 10.1007/978-3-211-99753-6 SpringerWienNewYork Preface The farmer folks around the world are facing acute problems in providing plants with required nutrients due to inadequate supply of raw materials, poor storage quality, indiscriminate uses and unaffordable hike in the costs of synthetic chemical fertilizers.
    [Show full text]
  • Identification of Culturable Endophytes in 'Champaka' Pineapple Grown In
    Vol. 8(26), pp. 3422-3430, 11 July, 2013 DOI: 10.5897/AJAR2012.2308 African Journal of Agricultural ISSN 1991-637X ©2013 Academic Journals Research http://www.academicjournals.org/AJAR Full Length Research Paper Identification of culturable endophytes in ‘Champaka’ pineapple grown in an organic system Olmar Baller Weber1*, Sandy Sampaio Videira2 and Jean Luiz Simões de Araújo2 1Embrapa Tropical Agroindustry, Dra. Sara Mesquita, 2270, Pici, 60511-110, Fortaleza, Ceará, Brazil. 2Embrapa Agrobiology, BR 465, km07, 23890-000, Seropédica, Rio de Janeiro, Brazil. Accepted 26 March, 2013 The objective of the study was to characterize culturable diazotrophic endophytic bacteria from ‘Champaka’ pineapple plants during the fruiting period in an organic system. Micropropagated plants were inoculated with the diazotrophic endophytic bacterium, strain AB 219a, during acclimatization phase. In the field, the plants were fertilized with different dosages and sources of organic compost. After 17 months of field cultivation, roots and stems were collected for the isolation of endophytes, DNA extraction, 16S rDNA amplification and molecular analysis. A total of nine bacterial groups were identified, and species Burkholderia silvatlantica, Azorhizobium caulinodans, Pantoea eucrina, Erwinia sp. and unculturable bacterium occurred in inoculated plants. In contrast, non-inoculated plants were associated endophytes related to Burkholderia cenocepacia, Azospirillum brasilense, Enterobacter oryzae, Erwinia sp. and Sphingobium yanoikuyae. Segments of 360 base pair from nifH gene were amplified from representative endophytes within identified species, except from the Pantoea eucrina strain AB 295. 16S rDNA phylogenetic analysis of endophytes isolated from inoculated plants revealed distinct families: Xanthobacteraceae, Burkholderiaceae and Enterobacteriaceae; and from non- inoculated plants, Rodospirillaceae and Sphingomonadaceae families were also identified.
    [Show full text]
  • GENETIC and PHENOTYPIC DIVERSITY of Azorhizobium Doebereinerae STRAINS
    GENETIC AND PHENOTYPIC DIVERSITY OF Azorhizobium doebereinerae STRAINS Ligiane Aparecida Florentino 1, Amanda Azarias Guimarães 2, Jacqueline Savana da Silva 3, Wesley Melo Rangel 4, Paula Marcela Duque Jaramillo 5, Fatima Maria de Souza Moreira 6 1 Professora Doutora da Universidade José do Rosário Vellano – Depto. de Ciências Agrárias, Alfenas, MG. 2 Pós-doutoranda em Ciência do Solo - Universidade Federal de Lavras (UFLA); Lavras, MG. 3 Mestre em Ciência do Solo - Universidade Federal de Lavras (UFLA); Lavras, MG. 4 Doutorando em Microbiologia Agrícola – Universidade Federal de Lavras (UFLA); Lavras, MG. 5 Pós-doutoranda da Embrapa Agroenergia; Brasília, DF. 6 Professora Titular do Departamento de Ciência do Solo – Universidade Federal de Lavras (UFLA); Lavras, MG. E-mail: [email protected]. Recebido em: 31/03/2015 – Aprovado em: 15/05/2015 – Publicado em: 01/06/2015 ABSTRACT Phenotypic studies based on cultural data and the analysis of total protein profiles have shown that Azorhizobium doebereinerae strains isolated from nodules of Sesbania virgata leguminous plants are highly similar. To identify better tests to discriminate among these strains, we examined genetic, using BOX-PCR, and phenotypic characteristics, such as growth at different temperatures, salt concentrations and pH values and resistance to various antibiotics. Strains representing the main genera of nitrogen-fixing nodulating bacteria were included as references in these tests. Strains isolated from S. virgata exhibited a high degree of genetic diversity indicated by a low similarity of BOX-PCR profiles: 36%. BR 5401 T, which was isolated from the same host but from other region, only had 26% similarity with the other A.
    [Show full text]
  • Enzyme Polymorphism of Azorhizobium Strains and Other Stem- and Root-Nodulating Bacteria Isolated from Sesbanìa Rostrata
    O INSTITUTPASTEUR/ELSEVIER Res. Microbiol. Paris 1993 1993, 144, 55-67 kc,f Enzyme polymorphism of Azorhizobium strains and other stem- and root-nodulating bacteria isolated from Sesbanìa rostrata G. Rinaudo (l), M.P. Fernandez (2), A. Effosse (2), B. Picard (3) and R. Bardin (2) (I) Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM), (2) Unite‘ de Recherche Associée au Centre National de Recherche Scientifique 1450, (’I 2, Laboratoire d’Ecologie microbienne du Sol, université Claude-Bernard-Lyon I, 69622 Villeurbanne Cedex (France), and (3) Laboratoire de Microbiologie, Hôpital Beaujon, 92110 Clichy (France) SUMMARY Relationships between bacterial groups nodulating Sesbania rostrata were evaluat- ed through examination of electrophoretic polymorphism of esterases and metabolic en- zymes. The following conclusions were drawn: (i)the differentiation of two genomic species within Azorhizobium strains and a group of non-identified strains (probably ßhizo- bium) was strongly supported by enzyme electrophoresis ;(i¡) esterases were more elec- trophoretically polymorphic than metabolic enzymes, since 35 and 11 electrophoretic types, respectively, were detected within the 57 strains studied ; (iii)strains isolated from stem or root nodules were genetically very similar and could not be differentiated; (iv) six Azorhizobium strains isolated from plants growing in saline soils could not be grouped separately from the other strains, which might be attributed to the adaptation of azorhizobia to epiphytic conditions; and (VIa comparative study of esterase patterns of azorhizobia showed that strains isolated in the Philippines probably originated in north- ern Senegal, but did not reveal a clear separation between strains originating from north- ern and central Senegal.
    [Show full text]
  • International Committee on Systematics Of
    ICSP - MINUTES de Lajudie and Martinez-Romero, Int J Syst Evol Microbiol 2017;67:516– 520 DOI 10.1099/ijsem.0.001597 International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium Minutes of the meeting, 7 September 2014, Tenerife, Spain Philippe de Lajudie1,* and Esperanza Martinez-Romero2 MINUTE 1. CALL TO ORDER (Chinese Agricultural University, Beijing, China) was later elected (online, November 2015) as a member of the subcom- The closed meeting was called by the Chairperson, E. Marti- mittee. It was agreed to invite representative scientist(s) from nez-Romero, at 14:00 on 7 September 2014 during the 11th Africa who have published validated novel rhizobial/agrobac- European Nitrogen Fixation Conference in Costa Adeje, Ten- terial species descriptions to become members of the subcom- erife, Spain. mittee. Several tentative names came up. MINUTE 2. RECORD OF ATTENDANCE MINUTE 6. THE HOME PAGE The members present were J. P. W. Young, E. Martinez- The website of the subcommittee can be accessed at http:// Romero, P. Vinuesa, B. Eardly and P. de Lajudie. K. Lind- edzna.ccg.unam.mx/rhizobialtaxonomy. It would be very ström was represented by S. A. Mousavi. All subcommittee useful to list genome sequenced type strains on the website. members had the opportunity to participate in the online discussions. K. Lindström, secretary of the subcommittee since 1996, resigned from this responsibility, but expressed MINUTE 7. GUIDELINES FOR THE her willingness to continue to act as an active subcommittee DESCRIPTION OF NEW TAXA member. P. Vinuesa agreed to act as a temporary secretary Some years ago, E.
    [Show full text]
  • Characterization of the Endosymbiotic Forms of Sinorhizobium Sp. Strain
    UNIVERSITÉ DE GENÈVE FACULTÉ DES SCIENCES Département de Botanique et Biologie Végétale Dr. Xavier Perret, MER ___________________________________________________________________ Characterization of the Endosymbiotic Forms of Sinorhizobium sp. Strain NGR234 THÈSE présentée à la Faculté des sciences de l’Université de Genève pour obtenir le grade de Docteur ès sciences, mention biologie par Nadia BAKKOU de Salé (Maroc) Thèse N° 4286 Genève Atelier d’impression ReproMail 2011 Remerciements Cette thèse a été réalisée au sein de l’Unité de Microbiologie dans le département de Botanique et Biologie végétale à l’université de Genève. Je tiens à remercier en premier lieu le Dr. Xavier Perret qui a dirigé cette thèse, pour sa disponibilité et pour sa confiance, qui m’ont permis de mener à bien ce projet. Je tiens également à remercier le Prof. Jurek Paszkowski, pour m’avoir permis de réaliser ma thèse dans ce département. Je remercie les membres du jury: le Dr. Cornelia Reimmann et le Prof. Hans-Martin Fischer, pour m’avoir fait l’honneur d’accepter de juger mon travail, pour leur remarques et conseils pertinents. Je tiens à associer à ces remerciements toutes les personnes qui ont participé à ce projet et sans qui ce travail n’aurait pas été possible. Je remercie donc le Dr. Michèle Crèvecœur, le Dr. Peter Mergaert et le Prof. Eva Kondorosi pour leur collaboration et leur aide scientifique et technique lors de la caractérisation morphologique et physiologique des bactéroides de Sinorhizobium sp. NGR234. Je remercie sincèrement Madame Yin Aung, le Dr. William Deakin et le Prof. Bill Broughton pour leur disponibilité, leurs conseils avisés et leur soutien tout au long de ce travail.
    [Show full text]
  • Nitrogen Fixation in Pure Culture by Rhizobia Isolated from Stem Nodules
    FEMS Microbiology Letters 68 (1990) 177-182 177 Published by Elsevier FEMSLE 03943 Nitrogen fixation in pure culture by rhzobia isolated from stem nodules of tropical Aeschynomene species Didier Alazard ORSTOM, Laboratoire de Microbiologie, Dakar, Sinkgal Received 17 November 1989 Accepted 28 November 1989 Key words: Aeschynomene; Asymbiotic nitrogen fixation; Rhizobium; Stem nodulation 1. SUMMARY demonstrated primarily in several strains belong- ing to the genus Bradyrhhizobium, B. japonicum Asymbiotic nitrogenase activity was investi- [l-41 and cowpea Bradyrhizobium spp. [3,5], in- gated in rhizobia strains isolated from stem and cluding the well characterized strains 32H1 [2,6-91 root nodules of several Aeschynomene species. All and CB756 [3,10,11]. Subsequently, a very limited isolates from stem-nodulating species were able to number of Rhizobium strains has been shown to develop nitrogenase activity ex planta in the pres- have some degree of acetylene-reducing ability ex ence or in the absence of combined nitrogen, planta: Rhizobium strain IHPlOO isolated from whereas root isolates from Aeschynomene species Cajanus cajun [lo], Parasponia-Rhizobium ANU related to the cowpea group of plants showed little 289 [12], and particular strains of R. leguminosarum or no activity. Nitrogenase activity in soft-agar [2,6,13], R. meliloti [14] and R. trifolii 0403 [15]. and in liquid cultures displayed by strains ORS310 None of these strains was able to grow at the and ORS322, isolated from stem nodules of A. expense of molecular nitrogen, except R. trifolii indica and A. afraspera respectively, was of the 0403, which was shown after a treatment involving same order of magnitude as that found for succinate to behave physiologically similar as nod- Azorhizobium caulinodans ORS571 and ten times ule-derived bacteroids.
    [Show full text]