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Product information NANOG 1-154aa Human, His-tagged, Recombinant, E.coli Cat. No. IBATGP1124 Full name: Homeobox protein NANOG NCBI Accession No.: NP_079141 Synonyms: Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48 Description: NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His- tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT Molecular Weight: 19.6 kDa (174aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) Purity: > 90% by SDS - PAGE Concentration: 0.25 mg/ml (determined by Bradford assay) 15% SDS-PAGE (3ug) Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ General references: Clark A T., et al. (2004) Stem Cells. 22:169-179. Chambers I., et al. (2003) Cell Res. 13:499-502. Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. For research use only. This product is not intended or approved for human, diagnostics or veterinary use. Immuno-Biological Laboratories, Inc. (IBL-America) 8201 Central Ave. NE, Suite P, Minneapolis, Minnesota 55432, USA Phone: +1 (763) - 780-2955 Fax.: +1 (763) - 780-2988 Email: [email protected] Web: www.ibl-america.com.

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Digestive and Liver Disease 47 (2015) 826–835 View metadata, citation and similar papers at core.ac.uk brought to you by CORE Contents lists available at ScienceDirect provided by Elsevier - Publisher Connector Digestive and Liver Disease journal homepage: www.elsevier.com/locate/dld Review Article Yes-associated protein in the liver: Regulation of hepatic development, repair, cell fate determination and tumorigenesis a d a,b,c a,b,c,∗ Quy Nguyen , Robert A. Anders , Gianfranco Alpini , Haibo Bai a Research, Central Texas Veterans Health Care System, Temple, TX, United States b Digestive Diseases Research Center, BaylorScott&White Healthcare, Temple, TX, United States c Department of Internal Medicine and Medical Physiology, Texas A&M Health Science Center, Temple, TX, United States d Department of Pathology, Johns Hopkins University, Baltimore, MD, United States a r t i c l e i n f o a b s t r a c t Article history: The liver is a vital organ that plays a major role in many bodily functions from protein production and Received 17 March 2015 blood clotting to cholesterol, glucose and iron metabolism and nutrition storage. Maintenance of liver Accepted 14 May 2015 homeostasis is critical for these essential bodily functions and disruption of liver homeostasis causes Available online 22 May 2015 various kinds of liver diseases, some of which have high mortality rate. Recent research advances of the Hippo signalling pathway have revealed its nuclear effector, Yes-associated protein, as an important Keywords: regulator of liver development, repair, cell fate determination and tumorigenesis. Therefore, a precise Hippo signalling pathway control of Yes-associated protein activity is critical for the maintenance of liver homeostasis.
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