PROLACTIN & DLS Science Cafe ANGIOGENESIS: SPECIAL LECTURE SIGNIFICANCE OF SERIES MICROHETEROGENEITY

Prof. Muralidhar has contributed significantly to biomedical sciences in the area of Biochemical Endocrinology. He has received numerous recognitions including fellowships from three major science academies. He is an outstanding and innovative teacher and has contributed to strengthening of all levels of biology education Prof. Muralidhar Kambadur from schools to universities. Prof. Muralidhar has assisted NCERT in bringing out new text JN Chair Professor books in biology for Class XI and XII, which University of have have been adopted by the CBSE for use across schools in the country. At the UG level, he has assisted the University of Delhi in formulating and piloting the academic bodies Organized by and is the Co-Chairman of SRF program Department of Life Sciences initiated by IASc, Bengaluru. At the PG level he Central University of has been the Chairman of the Curriculam Development Committee of UGC. He has also Thiruvarur, been a member of Examination Board of AIIMS for setting question papers for MBBS and MSc entrance exams. He has guided more than 50 Pro-VC PhD students and 3000 PG students, and also Chamber has 35 years of teaching experience. Prof. CUTN Muralidhar is currently serving many funding 29th Nov agencies and has been promoting science 10am enormously across the country. Prolactin and Angiogenesis-Significance of Micro-heterogeneity

Kambadur Muralidhar JN Chair, School of Life sciences Email: [email protected]

Abstract Prolactin (PRL) or Mammotropic hormone (MH) exhibits structural micro heterogeneity. The native hormone purified from buffalo pituitaries comprises size and charge isoforms. The isoform with higher pI (basic form) was found to possess higher bio potency in Nb2 rat lymphoma cell line growth assay the acidic isoform. The full length 22kDa isoform also had the full bio potency. Prolactin is known to produce more than 300 effects in vertebrates from fish to mammals. In order to understand this further, and to form a frame work for investigations, two hypotheses were proposed. One, that micro heterogeneous forms of prolactin should exhibit differential potency in a given bioassay during ontogeny. Two, that micro heterogeneous forms of Prolactin should exhibit different biological activities during phylogeny. Buffalo pituitary PRL was purified to homogeneity from freshly frozen pituitaries and characterized extensively. The cDNA corresponding to buffalo PRL was cloned and expressed as inclusion bodies in E.coli. The gene product was renatured and purified to homogeneity. It was as active as native hormone, if not more, in cell line based bioassay. It did not exhibit micro heterogeneity. Lower size isoforms of native PRL exhibited antiangiogenic activity in CAM assay. Intact PRL did not possess this activity. When the 22kDa intact PRL monomer was digested with Cathepsin D a number of fragments were obtained. The 14-kDa fragment exhibited anti-angiogenic activity in CAM assay, cell migration inhibition assay and wound healing assay.The amino acid sequence of buPRL monomer was deduced from cDNA nucleotide sequence.