Paper : 15 Molecular Cell Biology Module : 34 Methods for analysis of gene expression: analysis, DNA foot printing

Development Team

Principal Investigator: Prof. Neeta Sehgal Department of Zoology, University of Delhi

Co-Principal Investigator: Prof. D.K. Singh Department of Zoology, University of Delhi

Paper Coordinator: Prof. Kuldeep K. Sharma Department of Zoology, University of Jammu

Content Writer: Dr. Jasvinder Kaur, Dr. Poonam Sharma Gargi College, University of Delhi Ms Poornima Vishwakarma, Research Scholar, DU

Content Reviewer: Prof. Rup Lal Department of Zoology, University of Delhi

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ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing

Description of Module

Subject Name ZOOLOGY

Paper Name Zool 015: Molecular Cell Biology

Module Name/Title Methods for analysis of gene expression

Module ID M34: Promoter analysis, DNA foot printing

Keywords DNA Footprint Assay, DNase I, DNA foot print, Promoter characterization, Cleavage agent, Autoradiography

Glossary

Capillary electrophoresis: To modify the footprinting procedure to modernized detection means, the labelled DNA fragments are identified using capillary electrophoresis mechanism as a substitute of polyacrylamide gel

DNase I: A DNA- hydrolyzing enzyme that cuts DNA at random locations in a sequence independent manner

DNA Footprint Assay: Technique used to determine the binding site of a regulator to DNA which is typically at a promoter for a gene.

Foot print: The protected DNA region (bound by ) in a DNA footprinting experiment.

Hydroxyl radicals: When the iron salts are reacted with hydrogen peroxide, they are reduced to form free hydroxyl radicals. The DNA fragment is cleaved when the free hydroxyl molecule react with the DNA backbone.

In vivo footprinting: To analyze the protein-DNA interactions taking place in a cell at a certain time point, the in vivo footprinting technique utilized.

References

 http://molbiol-tools.ca/Promoters.htm  https://abc.med.cornell.edu/education/introtobio/t-promoter.html  https://en.wikipedia.org/wiki/DNA_footprinting

Further Reading

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ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing

 DNAase footprinting: Simple method for detection of protein-DNA binding specificity. David J. Galas and Albert Schmitz. 1978. Nucleic Acid Research.  Quantitative DNA Footprinting James C. Dabrowiak, Jerry Goodisman Brian Ward. Drug- DNA Interaction Protocols. Volume 90 of the series Methods in Molecular Biology™ pp 23-42. 1997. Humana Press. Totowa, NJ

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ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing