Paper : 15 Molecular Cell Biology Module : 34 Methods for analysis of gene expression: Promoter analysis, DNA foot printing Development Team Principal Investigator: Prof. Neeta Sehgal Department of Zoology, University of Delhi Co-Principal Investigator: Prof. D.K. Singh Department of Zoology, University of Delhi Paper Coordinator: Prof. Kuldeep K. Sharma Department of Zoology, University of Jammu Content Writer: Dr. Jasvinder Kaur, Dr. Poonam Sharma Gargi College, University of Delhi Ms Poornima Vishwakarma, Research Scholar, DU Content Reviewer: Prof. Rup Lal Department of Zoology, University of Delhi 1 ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing Description of Module Subject Name ZOOLOGY Paper Name Zool 015: Molecular Cell Biology Module Name/Title Methods for analysis of gene expression Module ID M34: Promoter analysis, DNA foot printing Keywords DNA Footprint Assay, DNase I, DNA foot print, Promoter characterization, Cleavage agent, Autoradiography Glossary Capillary electrophoresis: To modify the footprinting procedure to modernized detection means, the labelled DNA fragments are identified using capillary electrophoresis mechanism as a substitute of polyacrylamide gel DNase I: A DNA- hydrolyzing enzyme that cuts DNA at random locations in a sequence independent manner DNA Footprint Assay: Technique used to determine the binding site of a protein regulator to DNA which is typically at a promoter for a gene. Foot print: The protected DNA region (bound by proteins) in a DNA footprinting experiment. Hydroxyl radicals: When the iron salts are reacted with hydrogen peroxide, they are reduced to form free hydroxyl radicals. The DNA fragment is cleaved when the free hydroxyl molecule react with the DNA backbone. In vivo footprinting: To analyze the protein-DNA interactions taking place in a cell at a certain time point, the in vivo footprinting technique utilized. References http://molbiol-tools.ca/Promoters.htm https://abc.med.cornell.edu/education/introtobio/t-promoter.html https://en.wikipedia.org/wiki/DNA_footprinting Further Reading 2 ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing DNAase footprinting: Simple method for detection of protein-DNA binding specificity. David J. Galas and Albert Schmitz. 1978. Nucleic Acid Research. Quantitative DNA Footprinting James C. Dabrowiak, Jerry Goodisman Brian Ward. Drug- DNA Interaction Protocols. Volume 90 of the series Methods in Molecular Biology™ pp 23-42. 1997. Humana Press. Totowa, NJ 3 ZOOLOGY Molecular Cell biology Methods for analysis of gene expression: Promoter analysis, DNA foot printing .