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Product Sheet: Exosome Antibodies and Elisas

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Product Sheet: Exosome Antibodies and Elisas System Biosciences Accelerating discoveries through innovations Exosome Research Exosome Antibodies, Arrays and ELISAs Track, Verify and Quantitate Exosomes with Validated Antibody Systems Exosomes are small membrane vesicles secreted by most cell types in vivo and in Highlights vitro. Exosomes are found in cell culture media, blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs and proteins • Exosome antibodies for Westerns depending upon the tissue from which they are secreted. SBI's ExoELISA kits are designed for fast and quantitative analysis of well-characterized exosomal protein • Validated CD63, CD9, CD81 and Hsp70 markers: CD63, CD9, CD81 or Hsp70. The exosome antibody kits allow for the • Exosome ELISAs for quantitation confirmation of exosome recoveries and the ExoELISA kit enables the specific quantitation of CD63, CD9 or CD81 positive exosome microvesicles. The exosome • Measure exact number of exosome antibody and ExoELISA kits are fully compatible with exosomes isolated by SBI's particles isolated from your samples ExoQuick or ExoQuick-TC as well as ultracentrifugation methods. Exosome antibodies for Western blots Exosome antibody arrays to check recoveries For Western blotting analysis, we recommend The Exo-Check antibody array has 12 pre-printed spots and resuspending the exosome pellet in 1XRIPA features 8 antibodies for known exosome markers (CD63, CD81, buffer with the appropriate protease inhibitor ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101) and a GM130 cocktail. SBI offers individual antibodies for CD63, cis-Golgi marker to monitor any cellular contamination in your CD9, CD81 and Hsp70 as well as a Western blot exosome isolations. Your exosome preparations are lysed and sampler kit (Catalog# EXOAB-KIT-1) which then incubated with the array for the pre-printed antibodies to includes four exosomal marker antibodies: CD63, capture their respective exosome proteins. CD9, CD81, HSP70 (rabbit anti-human) and also includes a goat anti-Rabbit IgG HRP conjugated 123456 secondary antibody specifically tested for use in A Positive GM130 FLOT-1 ICAM ALIX CD81 exosomal protein analysis. The primary antibodies B CD63 EpCAM ANXA5 TSG101 Blank Positive are used at a 1:1,000 dilution and the HRP Positive GM130 FLOT1 ICAM ALIX CD81 secondary antibody at 1:20,000 dilution. (~53-70 kDa) (~53 kDa) (~26 kDa) (~28 kDa) kDa MW CD63 CD9 CD81 HSP70 CD63 EpCAM ANXA5 TSG101 Blank Positive 100 70 The positive control signals should provide a bright signal and this 55 will indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide signals 35 of varying degree, depending upon the source of the isolated 25 exosomes. The Blank spot should not have any signal and this serves as a background control. The GM130 control is to evaluate cellular contamination (if any) in your exosome preparations. The ExoQuick Exosome Serum Westernustom Analysis Services data above are from 300 ug of exosome proteins isolated using www.systembio.com/exoelisa ExoQuick-TC from human HT1080 lung sarcoma cell line media. ExoELISA™ Exosome Quantitation Kits Highly sensitive and quantitative ELISAs The ExoELISA kit is designed as a direct Enzyme-Linked ImmunoSorbent Assay (ELISA). The exosome particles and their proteins are directly immobilized onto the wells of the microtiter plate. After binding, wells are coated with a block agent to prevent non- specific binding of the detection antibody. The detection (primary) antibody is added to the wells for binding to specific antigen (e.g. CD63) protein on the exosomes. ExoELISA kits come with exosome standards to make calibration curves. Three primary antibody detection formats: CD63 Serum CD9 Serum • CD63 ExoELISA, catalog# EXOEL-CD63A-1 y=5E-11x + 0.117 y=6E-11x + 0.092 R2 = 0.971 R2 = 0.992 • CD9 ExoELISA, catalog# EXOEL-CD9A-1 • CD81 ExoELISA, catalog# EXOEL-CD81A-1 OD450 nm TMB Substrate OD450 nm HRP HRP Secondary Ab 0 5E+9 1E+10 1.5E+10 0 5E+9 1E+10 1.5E+10 # Exosome Particles # Exosome Particles CD81 Serum ExoELISA Standards CD63, CD9 y=7E-11x + 0.124 Calibrated by NanoSight or CD81 Ab 2 R = 0.993 90 NANONANOSIGHT N anoparticle Tracking Analysis (NTA) Version 2.1 BUILD 0329 Ag Exosomes directly adsorbed to ELISA Well OD450 nm Concentration (particles 1x10^6) Concentration 0 100 200 300 400 500 600 700 800 900 0 5E+9 1E+10 1.5E+10 Particle Size (nm) ExoELISA™ # Exosome Particles Plate A Horseradish Peroxidase enzyme (HRP) linked secondary antibody (goat anti-rabbit) is used for signal amplification and to increase assay sensitivity. A colorimetric substrate (Extra-sensitive TMB) is used for the assay read-out. The accumulation of the colored product is proportional to the specific antigen present in each well. The results are quantitated by a microtiter plate reader at 450 nm absorbance and calibrated by the exosome standards provided in the kit. The exosome standards are provided in number of exosome particles as determined by NanoSight dynamic light scattering measurements. The ExoELISA kits are compatible with exosomes isolated by ExoQuick, ExoQuick-TC or by ultracentrifugation techniques. time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066. System Biosciences, Inc. Toll Free: 888.266.5066 © 2014 System Biosciences, Inc. All rights 265 North Whisman Rd. Fax: 650-968-2277 reserved. System Biosciences and the System Biosciences logo are trademarks of System Mountain View, CA 94043 Email: [email protected] Biosciences, Inc. www.systembio.com.
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