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Bromocresol Purple Bromocresol Purple Catalog Number: 150518, 195080 Bromocresol Purple Structure :(free acid) Free Acid Sodium Salt Molecular Formula: C21H16Br2O5S C21H15Br2O5SNa Molecular Weight: 540.2 562.2 CAS # 115-40-2 62625-30-3 Synonym: 5',5''-Dibromo-o-cresolsulfonephthalein Physical Description: Reddish-brown crystalline powder (sodium salt); Orange to purplish-brown crystalline powder (free acid) pK: 6.3 Solubility: Free Acid: Soluble in water (20 mg/ml), ethanol (80 mg/ml) or methyl cellosolve (300 mg/ml). Sodium Salt: Soluble in water (80 mg/ml), ethanol (20 mg/ml) or methyl cellosolve (90 mg/ml). Description: Typically used as a pH indicator (5.2-6.8 : yellow to blue-purple). Bromocresol purple binds quantitatively with human serum1 albumin forming a stable complex with absorbance maximum at 600 nm. The intensity of the color produced is directly proportional to the albumin concentration in the sample. Availability: Catalog Number Description Size 195080 Bromocresol Purple 5 g 25 g 100 g 150518 Bromocresol Purple, Sodium Salt 5 g 25 g References: 1. Hill, P.G. and Wells, T.N.C., Ann. Clin. Biochem., v. 20, 264 (1983). 2. Orndorff, W.R. and Purdy, A.C., J. Am. Chem. Soc., v. 48, 2216 (1926). file:///C|/Users/Administrator/Desktop/新建文件夹/150518.htm[2013/3/4 13:44:01] Bromocresol Purple 3. "Reduction of reaction differences between human mercaptalbumin and human nonmercaptalbumin measured by the bromocresol purple method." Clin. Chim. Acta, v. 289:1-2, 69-78 (1999). 4. "A new photometric assay with bromocresol purple for testing in vitro antitrichomonal activity in aerobic environment." Arzneimittelforschung, v. 49:1, 51-55 (1999). 5. "Multivariate analysis of the association of bromocresol purple anion with bovine serum albumin." Biochim. Biophys. Acta, v. 1341:2, 207-216 (1997). 6. "Bromocresol purple dye-binding and immunoturbidimetry for albumin measurement in plasma or serum of patients with renal failure [letter]" Clin. Chem., v. 40:5, 844-845 (1994). 7. "Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity." Yeast, v. 9:11, 1207-1211 (1993). 8. "[Determination of serum albumin by a colorimetric micromethod using bromocresol purple]" Lab Delo., v. 2, 3- 6 (1987). 9. "Serum albumin by dye-binding: bromocresol green or bromocresol purple? The case for conservatism." Ann. Clin. Biochem., v. 25(pt 4), 417-421 (1988). 10. "Underestimation of serum albumin by the bromocresol purple method and a major endogenous ligand in uremia." Clin. Chim. Acta, v. 167:1, 89-96 (1987). 11. "Bromocresol purple dye-binding methods underestimate albumin that is carrying covalently bound bilirubin." Clin. Chem., v. 33:6, 821-823 (1987). 12. "Bromocresol purple method for serum albumin gives falsely low values in patients with renal insufficiency." Clin. Chim. Acta, v. 155:1, 83-87 (1986). 13. "Serum albumin assay with bromocresol purple dye." Scand. J. Clin. Lab Invest., v. 45:6, 561-564 (1985). 14. "Evaluation of a bromocresol purple method for the determination of albumin adapted to the DuPont aca discrete clinical analyzer." Clin. Biochem., v. 18:5, 290-296 (1985). 15. "Bromocresol puple dye-binding method for the estimation of serum albumin adapted to the SMA 12/60." Clin. Biochem., v. 18:5, 285-289 (1985). 16. "Determination of albumin with bromocresol purple using controlled-disperson flow analysis." Analyst, v. 110:6, 669-671 (1985). 17. "Automated stopped-flow analyser in clinical chemistry: determination of albumin with bromocresol green and purple." Clin. Chim. Acta, v. 149:2-3, 225-235 (1985). 18. "Albumin analysis in serum of haemodialysis patients: discrepancies between bromocresol purple, bromocresol green and electroimmunoassay." Ann. Clin. Biochem., v. 22 (pt 3), 304-309 (1985). 19. "Specific association of bromocresol purple anions with a magnesium complex of a phosphorylated intermediate during steady-state hydrolysis of ATP by the Mg2+ + Ca2+-dependent ATPase of sarcoplasmic reticulum." J. Biochem. (Tokyo), v. 91:2, 537-551 (1982). 20. "The introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure." J. Clin. Chem. Clin. Biochem., v. 22:10, 685-692 (1984). 21. "Bichromatic blanking in the bromocresol purple method for albumin in serum and plasma [letter]." Clin. Chem., v. 30:11, 1886-1887 (1984). 22. "Albumin determination with bromocresol purple: imprecision, comparison of methods and quality control." J. Clin. Chem. Clin. Biochem., v. 21:11, 679-682 (1983). 23. "Bromocresol purple and the measurement of albumin. Falsely high plasma albumin concentrations eliminated by increased reagent ionic strength." Ann. Clin. Biochem., v. 20 (pt 5), 264-270 (1983). 24. "Heparin does not interfere with the measurement of plasma albumin by bromcresol purple [letter]." Clin. Chem., v. 29:11, 1989-1990 (1983). 25. "Evaluation of bromocresol purple method for albumin as used with the aca [letter]." Clin. Chem., v. 29:8, 1564- 1565 (1983). file:///C|/Users/Administrator/Desktop/新建文件夹/150518.htm[2013/3/4 13:44:01].
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