OIE RL for CSF Prof. Dr. Volker Moennig Germany

OIE Reference Laboratory Reports Activities in 2012

Name of disease (or topic) for which you Classical Swine Fever are a designated OIE Reference Laboratory:

Address of laboratory Institute of Virology Department of Infectious Diseases University of Veterinary Medicine Hannover, Foundation Buenteweg 17 30559 Hannover, Germany

Tel.: Tel: (+49) 511-953-8841

Fax: Fax: (+49) 511-953-8898

e-mail address: [email protected] [email protected]

website: http://www.tiho-hannover.de/kliniken- institute/institute/institut-fuer-virologie-zentrum- fuer-infektionsmedizin/

Name (including Title) of Head of Prof. Dr. Volker Moennig (until October 2012) Laboratory (Responsible Official): Prof. Dr. Paul Becher (from October 2012)

Name (including Title and Position) of OIE Prof. Dr. Volker Moennig Reference Expert:

Date of submission to the OIE 14.01.2013

Instructions This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/

Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site: (http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):

ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test recommended by the OIE Total number of test performed last year*

Annual reports of OIE Reference Centres, 2012 1 OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »

Indirect diagnostic tests Nationally Internationally Virus neutralization test (CSFV/ BDV/BVDV) 2 148 Antibody ELISA (CSFV) 0 20 Direct diagnostic tests Nationally Internationally Virus isolation (CSFV/BDV/BVDV) 33 182 Real-time RT-PCR (Panpesti/ CSFV) 8 82 Genetic typing (CSFV phylogenetic analysis) 0 24 Antigen ELISA (CSFV) 0 24

* The numbers given in the table refer to tests of diagnostic specimen only. Additional tests were carried out in the framework of the duties of the reference laboratory: maintenance of serum and virus collection, inter-laboratory comparison tests, standardization of reference material and different related research projects. ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies? Yes No

3. Did your laboratory supply standard reference reagents to OIE Member Countries? Yes No

Amount Related Amount supplied Name of recipient OIE Type of reagent Produced/ supplied diagnostic internationally Member Countries available stored nationally test (ml, mg) and of institutions (ml, mg) Cloned cell Virus isolation stored 1 x 25 cm3 flask Spain culture PK 15 Virus Central Veterinary (flasks with Neutralization Laboratory replicating assay Algete/ Madrid monolayers) 1 x 25 cm3 flask Montenegro Diagnostic Veterinary Laboratory Podgorica CSFV, BDV, BVDV Virus Produced 8 x 1 ml Belgium strains and Neutralization National Reference isolates assay Laboratory Ukkel/ Brussels 2 x 1 ml Montenegro Diagnostic Veterinary Laboratory Podgorica 2 x 1 ml Spain CRESA Institute Barcelona

2 Annual reports of OIE Reference Centres, 2012 OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »

Amount Related Amount supplied Name of recipient OIE Type of reagent Produced/ supplied diagnostic internationally Member Countries available stored nationally test (ml, mg) and of institutions (ml, mg) 4 x 0,5 ml Germany National Reference Laboratory Greifswald- Island of Riems Complete serum Ab ELISA Produced 30 x 3 ml; Korea panel for ELISA 3 x 1 ml Bionote validation (1 Gyeonoggi-do panel with 31 different sera) 30 x 3 ml; Greece 3 x 1 ml Veterinary Institute of Infectious and Parasitic Disease Paraskevi, Athens 30 x 3 ml; United Kingdom 3 x 1 ml Laboratory Services Department, Weybridge/ Addlestone Complete serum Ab ELISA Produced 60 x 3 ml; Poland, National panel for ELISA 6 x 1 ml Veterinary Research validation (1 Institute, Pulawy panel with 31 different sera) Latvia PCR reference Real-time RT- 4x 2ml; National Reference Produced serum panel PCR 1 x 10 ml Laboratory Riga Monoclonal Virus Produced Hungary antibodies Neutralization 2 x 10 ml Veterinary Diagnostic directed against Assay Directorate, Budapest CSFV, BVDV or Virus Isolation BDV Poland, National 4 x 10 ml Veterinary Research Institute, Pulawy Switzerland 3 x 10 ml Institute of Virology Bern Lithuania National Institute of Food 2 x 10 ml and Veterinary Risk Assessment Vilnius 2 x 10 ml Finland Finnish Food Safety Authority EVIRA Helsinki

Annual reports of OIE Reference Laboratories, 2012 3 OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »

Amount Related Amount supplied Name of recipient OIE Type of reagent Produced/ supplied diagnostic internationally Member Countries available stored nationally test (ml, mg) and of institutions (ml, mg) Mexico Comisión México-Estados Unidos para la Prevención de la Fiebra 5 x 10 ml Aftosa y otras Enfermedades Exóticas de los Animales Del. Cuajimalpa Latvia Institute of Food Safety , 6 x 10 ml Animal Health and Environment “BIOR” Riga Monoclonal Virus Croatia antibodies Neutralization Croatian Veterinary directed against Produced 2x 10 ml Assay Institute CSFV, BVDV or Virus Isolation Zagreb BDV Reference sera Virus Produced Slovenia from Neutralization National Veterinary 3 x 10 ml experimentally assay Institute infected animals ELISA Ljubljana Belgium CODA-CERVA-VAR 10 x 1 ml Virology Ukkel/ Brussels Sweden 66 x 1 ml SVA Uppsala Finland Finnish Food Safety 2 x 1,5 ml Authority EVIRA Helsinki Germany National Reference 2 x 1,5 ml Laboratory Greifswald- Island of Riems Malaysia VET Food Agro 6 x 3 ml Diagnostics Petaling Jaya, Selangor Croatia Croatian Veterinary 3 x 1 ml Institute Zagreb

4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents? Yes No

5. Did your laboratory produce vaccines?

Yes No

6. Did your laboratory supply vaccines to OIE Member Countries? Yes No ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? Yes No

8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Yes No

Name of the new test or diagnostic method Description and References (Publication, website, etc.) or vaccine developed

Phylogenetic analysis of The short sequence lengths of the 5´NTR and the E2 gene fragments represent a limiting CSFV based on full-length factor for differentiation of closely related isolates and also for confidence levels of E2 encoding sequences proposed CSFV groups and subgroups. Analysis of full-length E2 encoding sequences proved to be more suitable for reliable and statistically significant phylogeny providing a solid basis for molecular epidemiology of CSF. Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., Pridotkas, G., Dirbakova, Z., Mojzis, M., and Becher P. (2012). Improved strategy for phylogenetic analysis of Classical swine fever virus based on full-length E2 encoding Sequences. Veterinary Research 43:50; http://www.veterinaryresearch.org/content/43/1/50.

ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 9. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes No

Name of OIE Member Country seeking Date No. samples received for No. samples received assistance (dd/mm) provision of diagnostic for provision of support (i.e. from confirmatory surveillance campaign) diagnoses Agricultural University Tirana, Albania 28/09 26 Regional Animal Health Office, Ho Chi Minh 9/11 10 City, Vietnam Institute of Food Safety, Animal Health and 16/11 18 Environment (BIOR), Riga, Latvia Institute of Food Safety, Animal Health and 28/11 28 Environment (BIOR), Riga, Latvia

10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes No

Name of the OIE Member Country How the advice was Purpose receiving a technical consultancy provided National Institute of Food and Veterinary Establishing peroxidase staining for Virus In-house training and Risk Assessment, Vilnius, Lithuania neutralization assay remote assistance Central Agricultural Office. Veterinary Technical advice on how to reduce toxic Remote assistance Diagnostic Directorate, Budapest, components that might lead to unspecific Hungary fluorescence in wild boar sera Department of Animal Health Ministry of Case definition for officially controlled diseases Remote assistance Agriculture, Brazil according to clinical, epidemiological and laboratory criteria Veterinary Institute of Infectious and Establishing new techniques in molecular biology In-house training and Parasitic Disease, Paraskevi-Athens, diagnostic methods in the NRL in Greece remote assistance Greece ARC-OVI-Transboundary Animal Diseases Information and technical advice on CSF Remote assistance Programme Onderstepoort, South Africa diagnostic real-time RT-PCR and preparation of sample material National Veterinary Research institute, Information on and practical training in the In-house training Pulawy, Poland performance of classical laboratory techniques (Virus isolation, Virus neutralization test) Institute of Food Safety, Animal Health Improvement of diagnostic methods for In loco assistance and Environment (BIOR), Riga, Latvia detection of CSFV, i.e. molecular biology.

ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes No

OIE Member Countries Title of Purpose of the Duration Partners (Institutions) involved the study study other than your country “CSFV_ 4 years Improve tools and Veterinary and Agrochemical Research Centre Belgium Belgium go DIVA” strategies for the National Veterinary Institute, Technical University of Denmark prevention and Denmark France control of French Food Safety Agency, France Hungary classical swine Central Agricultural Office, Directorate of Veterinary Netherlands fever Medicinal Products, Hungary Spain Central Veterinary Institute, Netherlands Italy Fort Dodge Animal Health, Spain Switzerland Friedrich-Loeffler-Institute, Federal Research Institute China for Animal Health, Germany Sweden Institute of Animal Health Umbria e Marche, Italy Institute for Virology and Immunoprophylaxis, Switzerland The National Game and Wildlife Agency, France Bioengineering Research Centre for Animal Disease Prevention and Control, China State Veterinary Institute, Sweden University Complutense of Madrid, Spain Helmholtz Centre for Environmental Research, Germany Spectos GmbH, Germany Institute of Animal Health of Abruzzo and Molise, Italy European 5 years Strengthening the Central Veterinary Institute (CVI), Lelystad, The Research cooperation Netherlands Netherlands Group between National Friedrich-Loeffler-Institute (FLI), Greifswald – Island of Germany (ERG, and International Riems, Germany United entitled Reference Institute for Animal Health (IAH), Newbury, UK Kingdom EPIZONE) laboratories in Animal Health and Veterinary Laboratories Agency Denmark the field of (AHVLA), Addlestone, Surrey, United Kingdom Sweden epizootic DTU Veterinæinstituttet (DTU Vet), Copenhagen, France diseases. Denmark Spain Statens Veterinarmedicinska Anstalt (SVA), Uppsala, Italy Sweden China Centre de coopération Internationale en Recherche Poland Agronomique pour le Développement (CIRAD), Turkey Monpellier, France Belgium Center of Animal Health, National Institute for Agriculture and Food Research and Technology (CISA- INIA), Valdeolmos, Spain Istituto Zooprofilattico Sperimentale delle Venezie, (IZSVe), Legnaro, Italy Lanzhou Veterinary Research Institute (LVRI), China National Veterinary Research Institute (NVRI) Pulawy, Poland FMD Institute Ankara (SAP), Ankara, Turkey VAR-CODA-CERVA (VAR-CODA), Ukkel, Belgium Istituto Zooprofilattico Sperimentale della Lombardia e dell´Emilia Romagna (IZS-Br), Brescia, Italy Harbin Veterinary Research Institute (CAAS), China

OIE Member Countries Title of Purpose of the Duration Partners (Institutions) involved the study study other than your country OIE 3 years Updating and Centro Nacional de Sandidad Agropecuaria, La Habana Cuba Laboratory Revision of (CENSA), Cuba Twinning Methods for Project diagnosis of CSF Evaluate sequences of Cuban local CSFV isolates and determination of genotype, for completing picture of Caribbean isolates Development, Evaluation and Implementation of a novel isothermal nucleic acid detection technique for CSFV Evaluation of new pen-side test for detection of CSF specific antibodies CRTI 09- 2 years Development of CIFA- National Center for Animal Disease, Winnipeg/ Canada 403TA ready-to-use Lethbridge, Canada USA laboratory and Nexogen, Inc., USA United field deployable Institute of Animal Health, United Kingdom Kingdom technology for DssFADDL-NVSL, rapid APHISUSDA, Plum Island, USA identification and genetic typing of multiple HC agents of swine DISCONTO 4 years Providing a http://www.discontools.eu/ OLS mechanism for focusing and prioritising research that ultimately delivers new and improved vaccines, pharmaceuticals and diagnostic tests

ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12. Did your Laboratory collect epizootiological data relevant to international disease control? Yes No

13. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes No

14. What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals:...... 09 b) International conferences:...... 08 c) National conferences:...... 09 d) Other:...... internet:1 books: 1 ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards

15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? Yes No

a) Technical visits:...... 4 b) Seminars:...... 0 c) Hands-on training courses:...... 3 d) Internships (>1 month):...... 0

Type of technical training Country of origin of the expert(s) No. participants from the provided (a, b, c or d) provided with training corresponding country

a Greece 1

c Poland 2

a Cuba 3

c Lithuania 1

ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16. Does your laboratory have a Quality Management System certified according to an International Standard? Yes No

Quality management system adopted ISO 17025

17. Is your laboratory accredited by an international accreditation body?

Yes No

Test for which your laboratory is accredited Accreditation body* Isolation, propagation and quantification of CSFV in cell culture DAKKS (ILAC-MRA) Detection of CSFV antigen by ELISA DAKKS (ILAC-MRA) Detection of antibodies directed against CSFV by ELISA DAKKS (ILAC-MRA) Detection of antibodies directed against CSFV by neutralization assay DAKKS (ILAC-MRA) Detection of CSFV genome using RT-PCR and subsequent preparation for genotyping DAKKS (ILAC-MRA) Detection of CSFV genome and of genome of other pestiviruses using real-time RT-PCR DAKKS (ILAC-MRA) Detection of CSFV genome using real-time RT-PCR with TaqMan probe DAKKS (ILAC-MRA) Detection of Pestivirus genome with RT-PCR DAKKS (ILAC-MRA) Detection of antibodies directed against Border Disease Virus (BDV) by neutralization DAKKS (ILAC-MRA) assay Detection of antibodies directed against Bovine Viral Diarrhoea Virus (BVDV) by DAKKS (ILAC-MRA) neutralization assay Detection of antibodies directed against Bovine Viral Diarrhoea Virus (BVDV) by ELISA DAKKS (ILAC-MRA) Detection of Bovine Viral Diarrhoea Virus (BVDV) antigen by ELISA DAKKS (ILAC-MRA) Virus isolation, propagation and quantification of Bovine Viral Diarrhoea Virus (BVDV) DAKKS (ILAC-MRA)

*The DAKKS (German accreditation body) accreditation falls within the scope of the multilateral agreements (MLA/MRA) of the European co-operation for Accreditation (EA) and the international accreditation organisations IAF and ILAC.

18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) Yes No ToR: To organise and participate in scientific meetings on behalf of the OIE 19. Did your laboratory organise scientific meetings on behalf of the OIE? Yes No

National/ Title of event Co-organiser Date Location No. International (mm/yy) Participants International Workshop on Laboratory CISA-INIA, 05/12 Hannover, 93 Diagnosis of African and Madrid, Spain, Germany Classical Swine Fever DG-SANCO National Expert Workshop Swine LAVES, Germany; 11/12 Hannover, 21 Fever: FLI, National RL Germany Clinical signs, epidemiology Germany; and disease control AKNZ, Germany

20. Did your laboratory participate in scientific meetings on behalf of the OIE? Yes No

Title of event Date Location Role (speaker, presenting Title of the work (mm/yy) poster, short presented communications) Meeting of the Task 03/12 Brussels, Speaker Summary of the conclusions Force (TF) for Belgium (V. Moennig) and recommendation of the monitoring Animal fourth meeting held by the Disease eradication in sub-group on Classical Swine the member states fever (CSF) 22nd Annual Meeting 03/12 Essen, Germany Speaker Morphogenesis of of the Society for (S. Schmeiser) Pestiviruses Virology Poster Classical Swine Fever – A (A. Postel) New Strategy for Molecular Epidemiology Poster Evolution of chimeric (P. Becher) pestiviruses by nonhomologous RNA recombination between different virus species Poster Crucial role of two amino (D. Meyer) acids on the antibody binding of the Classical swine fever virus glycoprotein Erns Poster 3´Terminal Phoshate and 5 (S. Austermann-Busch) ´Terminal Hydroxyl Groups of RNA Molecules Enhance RNA Recombination During Viral Infection Workshop Laboratory 05-06/12 Hannover Speaker Imroved Strategy for Diagnosis of African Germany (A. Postel) Phylogenetic Analysis of Swine Fever and Classical Swine Fever Virus Classical Swine Fever based on full-length E2 encoding Sequences Workshop Laboratory 05-06/12 Hannover Speaker Is isolated RNA of CSFV Diagnosis of African Germany (S. Schmeiser) infectious for pigs? Swine Fever and Classical Swine Fever Scientific conference 06/12 Novi Sad, Serbia Speaker Molecular epidemiology of “Prevention of (S. Schmeiser) classical swine fever virus Classical Swine Fever isolates from South-eastern in the border region Europe Croatia - Serbia (“Stop CSF”) CSFV-goDIVA Meeting 06/12 Visegrad, Speaker Serum sample panel for the Hungary (K. Pannhorst) evaluation of selected ELISA systems within an inter- laboratory comparison test IX International 09/12 Madrid, Spain Poster Molecular Characterization Congress of Veterinary (P. Becher) of Border Disease Virus Virology Strain Aveyron ESVV Introduced Intelligence 09/12 Beijing, China Speaker Diagnosis and Molecular project of State (P. Becher) Epidemiology of Classical Administration of Swine Fever Foreign Expert Affairs China (SAFEA)

Title of event Date Location Role (speaker, presenting Title of the work (mm/yy) poster, short presented communications) International 09/12 Beijing, China Speaker Variability of Classical Swine Workshop of (P. Becher) Fever Virus and related Prevention and viruses: Implication for Control Strategies of Diagnosis and Control of CSF Classical Swine Fever 4th China Congress of Veterinary Medicine World Veterinary 09/12 Ames, USA Speaker Classical Swine Fever Association Workshop (V. Moennig) “Vaccines and Diagnostics for Transboundary Animal Diseases” IPA Multibeneficiary 10/12 Belgrade, Invited consultant experts in Invited consultant experts in Project “Support for Republic of CSF diagnosis and CSF diagnosis and the control/ Serbia epidemiology epidemiology eradication of animal (P. Becher, A. Postel) diseases”, second regional Workshop DISCONTOOLS 11/12 Brussels, Speaker Epizootic – Classical Swine Conference “Fighting Belgium (V. Moennig) Fever animal diseases identifying research priorities” Expert Workshop 11/12 Hannover, Speaker Molecular Epidemiology of Classical Swine Fever: Germany (P. Becher) Classical Swine Fever Clinical Signs, epidemiology and Speaker Handling and Shipment of disease control (S. Austermann-Busch) CSF suspicious sample material CSFV_goDIVA Meeting 11/12 Lelystad, The Speaker Preliminary results of the Netherlands (K. Pannhorst) evaluation of selected ELISA systems within an inter- laboratory comparison test

ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Yes No

22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes No

Purpose of the proficiency tests: Role of your Reference No. Participating OIE Ref. (validation of a diagnostic protocol: Laboratory (organiser/ participa Labs/ organising OIE specify the test; quality control of participant) nts Ref. Lab. vaccines: specify the vaccine type, etc.) Validation: Organiser 56 OIE RL for CSF, Poland Real-time RT-PCR OIE RL for CSF, United Conventional RT-PCR Kingdom Virus Neutralization assay OIE RL for CSF, Canada Virus Isolation Antibody ELISA Antigen ELISA

23. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Yes No

Name(s) of relevant OIE Title of the project or contact Scope Reference Laboratories European Research Group (ERG, Strengthening the cooperation between National OIE RL for CSF, Poland and entitled EPIZONE) and International Reference laboratories in the OIE RL for CSF, UK field of Epizootic diseases

ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results. 24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes No

No. participating Participating OIE Member Purpose for inter-laboratory test comparisons1 laboratories Countries Austria; Belgium; BIH; Brazil; Bulgaria; Chile; Croatia; Cuba; Cyprus; Czech Reference Laboratory as Organiser: Republic; Denmark; Dominican Laboratory capability to conduct specific diagnostic Republic; Estonia; Finland; France; tests: Germany; Greece; Haiti; Hungary; Real-time RT-PCR (CSF specific and Panpesti) Ireland; Italy; Jamaica; Latvia; Conventional RT-PCR 56 Lithuania; Luxembourg; Mexico; Virus Neutralization assay (CSFV; BVDV; BDV) Montenegro; Norway; Portugal; PR Virus Isolation China; FYROM; Romania; Russia; Antibody ELISA Serbia; Slovakia; Slovenia; South Africa; Antigen ELISA Spain; Sweden; Switzerland; The Netherlands; Trinidad and Tobago; Ukraine; United Kingdom; USA Reference Laboratory as Participant Laboratory capability to conduct CSF serological diagnostics Organiser: National Reference Laboratory for CSF, Germany

1 See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3

No. participating Participating OIE Member Purpose for inter-laboratory test comparisons laboratories Countries Reference Laboratory as Participant Laboratory capability to conduct CSF serological diagnostics Organiser: National Reference Laboratory for CSF, France

ToR: To place expert consultants at the disposal of the OIE 25. Did your laboratory place expert consultants at the disposal of the OIE? Yes No

Kind of consultancy Location Subject (facultative ) Provision of comments on review of OIE Amendment to Chapter 2.8.3. Standards classical swine fever (hog cholera) Attendance at conference Fleesensee, Germany 25th Conference of the OIE Regional Commission for Europe” Chair of the CSF Taskforce of DG Sanco Brussels, Belgium Summary of the conclusions and (V. Moennig) recommendation of the fourth meeting held by the sub-group on Classical Swine fever (CSF)

Annex : List of Publications Scientific publications in peer-reviewed journals:

Austermann-Busch, S., and Becher, P. (2012). RNA structural elements determine frequency and sites of nonhomologous recombination in an animal plus-strand RNA virus. Journal of Virology 86, 7393–7402.

Becher, P., Schmeiser, S., Oguzoglu, T.C., and Postel, A. (2012). Complete genome sequence of a novel pestivirus from sheep. Journal of Virology 86, 11412.

Blome, S., Meindl-Böhmer, A., Nowak, G., and Moennig, V. (2012). Disseminated intravascular coagulation does not play a major role in the pathogenesis of classical swine fever. Veterinary Microbiology. [Epub ahead of print]. PMID: 23107658

Kautto, A.H., Alenius, S., Mossing, T., Becher, P., Belák, S., and Larska, M. (2012). Pestivirus and alphaherpesvirus infections in Swedish reindeer (Rangifer tarandus tarandus L.). Veterinary Microbiology. 156, 64–71.

Lattwein, E., Klemens, O., Schwindt, S., Becher, P., and Tautz, N. (2012). Pestivirus virion morphogenesis in the absence of uncleaved nonstructural protein 2-3. Journal of Virology 86, 427–437.

Meyer, D., Aebischer, A., Müller, M., Grummer, B., Greiser-Wilke, I., Moennig, V., and Hofmann, M.A. (2012). New insights into the antigenic structure of the glycoprotein E(rns) of classical swine fever virus by epitope mapping. Virology 433, 45–54.

Postel, A., Jha, V.C., Schmeiser, S., and Becher, P. (2012). First molecular identification and characterization of classical swine fever virus isolates from Nepal. Archives of Virology Sep 14. [Epub ahead of print]. PMID: 22975985.

Postel, A., Schmeiser, S., Bernau, J., Meindl-Boehmer, A., Pridotkas, G., Dirbakova, Z., Mojzis, M., and Becher, P. (2012). Improved strategy for phylogenetic analysis of classical swine fever virus based on full- length E2 encoding sequences. Veterinary Research. 43, 50.

Postel, A., Schmeiser, S., Perera, C.L., Rodríguez, L.J.P., Frias-Lepoureau, M.T., and Becher, P. (2012). Classical swine fever virus isolates from Cuba form a new subgenotype 1.4. Veterinary Microbiology [Epub ahead of print]. PMID: 22902191

Internet: http://public.csf-wildboar.eu/Default.aspx

Book chapters:

Simmonds, P., Becher, P., Collett, M.S., Gould, E.A., Heinz, F.X., Meyers, G., Monath, T., Pletnev, A., Rice, C.M., Stiasny, K., et al. (2012). Family Flaviviridae. In Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Edited by A.M.Q. King et al. Academic Press, San Diego, USA.

Presentations at national and international conferences and meetings:

Austermann-Busch S. and Becher P. (2012). 3´ Terminal Phosphate and 5´ Terminal Hydroxyl Groups of RNA Molecules Enhance RNA Recombination During Viral Infection. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th of March 2012.

Austermann-Busch S. (2012). Handling and Shipment of CSF suspicious sample material. Expert-Workshop Classical Swine Fever: Clinical Signs, epidemiology and disease control, Hannover, Germany, 26th –30th of November, 2012.

Becher P., Gallei A. 2012. Evolution of chimeric pestiviruses by nonhomologous RNA recombination between different virus species. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th of March 2012.

Becher P. (2012). Variability of Classical Swine Fever Virus and related viruses: Implications for Diagnosis and Control of CSF. International Workshop of Prevention and Control Strategies of Classical Swine Fever, 4th China Congress of Veterinary Medicine, Beijing, China 3rd-4th of September, 2012.

Becher P. (2012). Molecular Epidemiology of Classical Swine Fever. Expert-Workshop Classical Swine Fever: Clinical Signs, epidemiology and disease control, Hannover, Germany, 26th –30th of November, 2012.

Meyer D., Moennig V. and Greiser-Wilke I. (2012). Crucial role of two amino acids on the antibody binding of Classical swine fever virus glycoprotein Erns. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th of March 2012.

Moennig V. (2012) Summary of the conclusions and recommendation of the fourth meeting held by the sub- group on Classical Swine fever (CSF). Meeting of the Task Force for monitoring Animal Disease eradication in the member states, Brussels, Belgium, 1st of March, 2012.

Moennig V. (2012). Classical Swine Fever. Workshop “Vaccines and Diagnostics for Transboundary Animal Diseases”, Iowa State University, Ames, IA, USA, 17th-19th of September 2012.

Moennig V. and Becher P. (2012). Classical Swine Fever –Scoring Exercise. Disontools Conference “Fighting animal diseases identifying research priorities”, Brussels, Belgium, November 15th, 2012.

Pannhorst K., Meyer D., Meindl-Boehmer A., Schmeiser S., Staubach C. and Blome S. (2012). Serum sample panel for the evaluation of selected ELISA systems within an inter-laboratory comparison test. CSFV_goDIVA Meeting, Visegrad, Hungary, 6th- 7th of June, 2012.

Pannhorst K., Meyer D., Staubach C., Fröhlich A. and Sonnenburg J. (2012). Preliminary results of the

evaluation of selected ELISA systems within an inter-laboratory comparsion test. CSFV_goDIVA Meeting, Lelystad, The Netherlands, 28th- 29th of November, 2012.

Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., Pridotkas G., Dirbakova Z., Mojzis M. and Becher P. (2012). Classical Swine Fever – A New Strategy for Molecular Epidemiology. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th of March 2012.

Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., and Becher P. (2012). Improved Strategy for Phylogenetic Analysis of Classical Swine Fever Virus based on full-length E2 encoding Sequences. Workshop Laboratory Diagnosis of African Swine Fever and Classical Swine Fever, Hannover, Germany, May 30 th – June 1st, 2012.

Schmeiser S. (2012). Morphogenesis of Pestiviruses. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th of March 2012.

Schmeiser S. (2012). Is isolated RNA of CSFV infectious for pigs? Workshop Laboratory Diagnosis of African Swine Fever and Classical Swine Fever, Hannover, Germany, May 30th – June 1st, 2012.

Schmeiser S. (2012). Molecular epidemiology of classical swine fever virus isolates from South-eastern Europe. Scientific conference “Prevention of Classical Swine Fever in the border region Croatia - Serbia (“Stop CSF”), Novi Sad, Republic of Serbia, 7th- 8th of June, 2012.

Vilcek S., Leskova V., Postel A., and Becher P. (2012). Molecular characterization of Border Disease Virus strain AVEYRON ESVV. IX International Congress of Veterinary Virology, Madrid, Spain 4 th – 7th September 2012.

16 Annual reports of OIE Reference Centres, 2012