UCSF Lab Standard Operating Procedure s29

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class: Aristolochic Acid

Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Aristolochic acid is a mutagen and carcinogen. May implicated in the cause of gastric cancer.

May be harmful by inhalation, ingestion, or skin absorption. Can cause eye and skin irritation.

Also known as 8-Methoxy-3,4-methylenedioxy-10-nitrophenanthrene-1-carboxylic acid.

Is a family of plant compounds with adverse effects. It is found primarily in the plant Aristolochia, but may also be present in other botanicals.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 313-67-7

Class: Carcinogen, Mutagen

Molecular Formula: C17H11NO7

Form (physical state): solid

Color: N/A

Boiling point: 615.5°C @760mmHg

Aristolochic Acid 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Potential Hazards/Toxicity

Aristolochic acid is a mutagen and carcinogen. May implicated in the cause of gastric cancer.

May be harmful by inhalation, ingestion, or skin absorption. Can cause eye and skin irritation. Entry into the bloodstream may produce systemic injury with harmful effects.

Aristolochic acid containing products was associated with kidney damage or kidney failure. Some patients have developed certain types of cancers.

No permissible exposure limit data is available.

No acute toxicity data is available.

Engineering Controls

Handle using a chemical fume hood with good ventilation and electrically grounded lines and equipment.

Personal Protective Equipment (PPE)

Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand Protection Gloves must be worn. Use proper glove removal technique to avoid any skin contact. Nitrile gloves are recommended.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Aristolochic acid.

Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/

Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles. Face shield is also recommended.

Skin and Body Protection Flame resistant lab coats must be worn and be appropriately sized for the individual and buttoned to their full length. Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.

Hygiene Measures Wash thoroughly and immediately after handling. Remove any contaminated clothing and wash before reuse.

First Aid Procedures

Aristolochic Acid 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) If inhaled Move into the fresh air immediately and give oxygen. If not breathing give artificial respiration. Seek medical attention immediately.

In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash any contaminated clothing before reuse. Thoroughly clean shoes before reuse. Seek medical attention immediately.

In case of eye contact Check for and remove any contact lenses. Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Seek immediate medical attention and continue eye rinse during transport to hospital.

If swallowed Do NOT induce vomiting unless directed by medical personnel. Never give anything by mouth to an unconscious person. Seek medical attention immediately.

Special Handling and Storage Requirements

Precautions for safe handling: Avoid contact with skin and eyes and inhalation. Avoid inhalation of vapor or mist. Avoid formation of dust.

Conditions for safe storage: Recommended storage temperature: 2 - 8 °C. Keep in a dry place. Keep container tightly closed in a cool, dry, and well ventilated. Keep away from incompatible materials and conditions. Keep cool and protect from sunlight.

Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency

Aristolochic Acid 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP: ______(g , ml) to ______(g, ml)

Temperature range covered by this SOP: __ °C – __ °C

General Overview and Purpose: Enter the experimental purpose

Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

Aristolochic Acid 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Any deviation from this SOP requires approval from the Principal Investigator.

Aristolochic Acid 5 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)