Supporting Information （Environmental Science and Pollution Research）: Comparison study on species sensitivity distribution of species from China and America

Xiaonan Wang · Zhenguang Yan · Zhengtao Liu · Cong Zhang · Weili Wang · Handong Li

X. Wang · Z. Yan () · Z. Liu () · W. Wang · H. Li State Key Laboratory of Environmental Criteria and Risk Assessment, State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, An wai da yang fang 8, Chaoyang District, Beijing 100012, China

X. Wang College of Water Sciences, Beijing Normal University, Beijing 100875, China

 Corresponding author: Zhengtao Liu, Zhenguang Yan P.R. China Tel: +86-10-84915175 Fax: +86-10-84915173 E-mail: [email protected], [email protected]

C. Zhang School of Environment, Beijing Normal University, Beijing 100875, China Table S1 LC50/EC50 for species in China for As3+ Taxa Species scientific name LC50/EC50 (μg L-1) Invertebrate Caenorhabditis elegans 179000 Daphnia magna 2518.46 Ceriodaphnia dubia 1448 Ceriodaphnia reticulata 981 Simocephalus serrulatus 1400 Daphnia pulex 1800 Chironomus sp. 33217.48 Polypedilum sp. 68950.15 vertebrate Carassius auratus 44900 Ictalurus punctatus 32824.38 Lepomis macrochirus 31954.15 Oncorhynchus kisutch 14500 Oncorhynchus mykiss 18451.05 Thymallus arcticus 11600

Table S2 LC50/EC50 for species in America for As3+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Ceriodaphnia reticulata 1511 Simocephalus vetulus 1700 Simocephalus 812 Serrulatus Daphnia magna 4449 Daphnia pulex 1626 Gammarus pseudolimnaeus 874 Tanytarsus dissimilis 97000 Pteronarcys californica 22040 Aplexa hypnorum 24500 vertebrate Lepomis macrochirus 41760 Carassius auratus 26040 Jordanella floridae 20130 Ictalurus punctatus 18100 Salvelinus fontinalis 14960 Oncorhynchus mykiss 13340 Pimephales promelas 14065

2 Table S3 LC50/EC50 for species in China for Cr6+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Diaphanosoma brachyurum 3.00 Daphnia hyalina 22.00 Daphnia magna 24.20 Daphnia pulex 36.30 Simocephalus serrulatus 40.90 Ceriodaphnia reticulata 45.20 Daphnia carinata 61.34 Daphnia obtusa 118.45 Ceriodaphnia dubia 144.50 Macrobrachium nipponense 2241.00 Tubifex tubifex 2462.81 Bryocamptus minutus 3560.00 Attheyella crassa 3820.00 Philodina roseola 7183.29 Lumbriculus variegatus 12095.45 Gobiocypris rarus 111240.00 vertebrate Aristichthys nobilis 10700 Pelteobagrus fulvidraco 15790 Rana chensiensis 28810 Gasterosteus aculeatus 33985.29 Bufo melanostictus 49290 Bufo viridis Laurenti 50000 Monopterus albus 60550 Rana hexadactyla 65261.01 Gobiocypris rarus 98722 Clarias batrachus 162390 Thymallus arcticus 169115.35 Carassius auratus 171149.06 Cyprinus carpio 346700

Table S4 LC50/EC50 for species in America for Cr6+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Hyalella azteca 630 Crangonyx pseudogracilis 583 Gammarus pseudolimnaeus 67.1

3 Orconectes rusticus 176000 Ceriodaphnia reticulata 45.1 Ceriodaphnia reticulata 40.9 Simocephalus serrulatus 32.3 Daphnia magna 23.07 Daphnia pulex 36.3 Neophasganophora capitata 1870000 Enallagma aspersum 140000 Chironomus tentans 61000 Tanytarsus dissimilis 57300 Lophopodella carteri 1560 Pectinatella magnifica 1440 Plumatella emarginata 650 Physa heterostropha 23010 vertebrate Lepomis cyanellus 114700 Lepomis macrochirus 132900 Carassius auratus 119500 Pomoxis annularis 72600 Oncorhynchus mykiss 69000 Notropis atherinoides 48400 Notropis chrysocephalus 85600 Notropis stramineus 74600 Campostoma anomalum 51250 Salvelinus fontinalis 59000 Ericymba buccata 49600 Pimephalas notatus 54225 Poecilia reticulata 30000 Pimephales promelas 41050 Etheostoma nigrum 46000 Perca flavescens 36300 Morone saxatilis 30450

4 Table S5 LC50/EC50 for species in China for Hg2+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Moina macrocopa 1.00 Daphnia cucullata 2.35 Daphnia obtusa 2.80 Ceriodaphnia reticulata 2.90 Daphnia magna 5.48 Daphnia hyalina 5.50 Ceriodaphnia dubia 8.80 Macrobrachium nipponense 13.10 Daphnia pulex 24.56 Moina dubia 27.00 Daphnia rosea 36.28 Chydorus sphaericus 43.17 Branchiura sowerbyi 80.00 Lumbriculus variegatus 100.00 Nais communis 160.00 Limnodrilus hoffmeisteri 180.00 Asellus aquaticus 199.00 Aedes aegypti 290.00 Tubifex tubifex 418.33 Eriocheir sinensis 442.30 Spirosperma nikolskyi 500.00 Dugesia japonica 647.00 Culex pipiens 1400.00 Ephemerella subvaria 2000.00 Chironomus plumosus 3057.06 vertebrate Ictalurus punctatus 0.30 Carassius auratus 0.70 Rana hexadactyla 51.00 Tanichthys albonubes 75.00 Bufo melanostictus 89.81 Rana limnocharis 103.00 Spinibarbus sinensis 141.00 Thymallus arcticus 164.41

5 Heteropneustes fossilis 188.17 Rhodens sinensis 193.00 Pseudorasbora parva 244.00 Ctenopharyngodon idellus 362.00 Rhodeus ocellatus 362.10 Microhyla ornata 365.15 Clarias batrachus 380.00 Rana chensinensis 488.00 Cyprinus carpio 527.72 Anabas testudineus 641.00 Monopterus albus 670.00 Misgurnus bipartitus 692.80 Bufo viridis 700.00 Rana tigrina 17164.79

Table S6 LC50/EC50 for species in America for Hg2+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Daphnia magna 3.7 Daphnia pulex 2.9 Ceriodaphnia reticulata 2.9 Gammarus sp. 10 Orconectes limosus 50 Faxonella clypeatus 20 Acroneuria lycorias 2000 Ephemerella subvaria 2000 Hydropsyche betteni 2000 Caddisfly,(Unidentified) 1200 Damselfly,(Unidentified) 1200 Chironomus sp. 20 Chironomus riparius 750 Nais sp. 1000 Quistadrilus multisetosus 250 Rhyacodrilus montana 240 Spirosperma ferox 330 Spirosperma rikolskyi 500 Limnodrilus hoffmeisteri 180 Tubifex tubifex 140 Stylodrilus heringianus 140 Varichaeta pacifica 100 Branchiura sowerbyi 80 Aplexa hypnorum 370 Amnicola sp. 80 vertebrate Tilapia mossambica 1000

6 Clarias batrachus 375 Oncorhynchus kisutch 240 Oncorhynchus mykiss 275 Pimephales promelas 163 Lepomis macrochirus 160 Gambusia affinis 203 Poecilia reticulata 28

Table S7 LC50/EC50 for species in China for Cd2+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Palaemonetes pugio 1.1 Simocephalus serrulatus 13.1 Macrobrachium nipponnensic 19.0 Gammarus pulex 20.0 Simocephalus vetulus 24.0 Daphnia magna 39.1 Gammarus lacustris 40.1 Ceriodaphnia dubia 54.8 Brachionus calyciflorus 70.0 Moina macrocopa 71.3 Asellus aquaticus 79.8 Physa fontinalis 80.0 Daphnia pulex 105.7 Ceriodaphnia reticulata 110.1 Hydra vulgaris 115.2 Eurytemora affinis 120.0 Hydra viridissima 210.0 Hydra oligactis 320.0 Chironomus plumosus 400.0 Alona affinis 546.0 Daphnia obtusa 580.0 Daphnia carinata 651.1 Lumbriculus variegatus 780.0 Procambarus clarkii 1199.6 Tubifex tubifex 1678.9 Hydra pseudoligactis 1810.0 Limnodrilus hoffmeisteri 2400.0 Bellamya purificata 3390.0 Eriocheir sinensis 3453.4 Sinopotamon henanense 10170.0 Propsilocerus akamusi 55374.9

7 Branchiura sowerbyi 58020.0 vertebrate Aristichthys nobilis 280.0 Cichlasoma managuense 480.0 Carassius auratus 748.0 Salmo trutta 1.4 Ctenopharyngodon idellus 2422.9 Rana limnochari 2820.8 Rana nigromaculata 3100.0 Cyprinus carpio 3801.9 Megalobrama pellegrini 4440.0 Tanichthys albonubes 4447.0 Cyprinus carpiovar 4560.0 Pseudorosbora parva 6294.0 Gasterosteus aculeatus 6500.0 Bufo bufogargarizans 7462.7 Gobiocypris rarus 12240.0 Channa striata 19400.0 Misgurnus anguillicaudatus 56500.0

Table S8 LC50/EC50 for species in America for Cd2+ Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrate Orconectes virilis 12755 Simocephalus serrulatus 33.2 Simocephalus vetulus 27.03 Daphnia magna 14.2 Daphnia pulex 31.43 Rhyacodrilus montana 7921 Stylodrilus heringianus 6915 Spirosperma ferox 4401 Spirosperma nikolskui 5658 Varichaeta pacifica 4778 Tubifex tubifex 4024 Quistradilus multisetosus 4024 Amnicola sp. 3800 Branchiura sowerbyi 3018 Ephemerella grandis 2310 Limnodrilus hoffmeisteri 2137 Nais sp. 1700 Crangonyx pseudogracilis 1700 Chironomus sp. 1200 Asellus bicrenata 401 Plumatella emarginata 221.9 Physa gyrina 156.9

8 Pectinatella magnifica 142.5 Aplexa hypnorum 104.0 Gammarus pseudolimnaeus 80.33 Gammarus sp. 70.00 Ceriodaphnia reticulata 48.28 Lirceus alabamae 42.8 Moina macrocopa 40.78 Lophopodella carteri 30.54 vertebrate Carassius auratus 8325 Pimephales promelas 30.50 Gambusia affinis 7685 Gasterosteus aculeatus 4977 Poecilia reticulata 3570 Catostomus commersoni 3514 Jordanella floridae 2888 Ptychocheilus oregonensis 2400 Lepomis cyanellus 2399 Lepomis gibbosus 1347 Lepomis macrochirus 4249 Anguilla rostrata 736 Cyprinus carpio 215.5 Oncorhynchus kisutch 6.48 Oncorhynchus tshawytscha 4.254 Oncorhynchus mykiss 5.78 Morone americana 7544 Morone saxatilis 2.682 Salmo trutta 1.647 Fundulus diaphanus 98.79

9 Table S9 LC50/EC50 for species in China for nitrobenzene Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrate Daphnia magna 38.99 Daphnia carinata 39.8 Macrobrachium nipponense 0.039 Limnodrilus hoffmeisteri 116.12 Cipangopaludina cahayensis 103.3 Lymnaea stagnalis 64.5 Culex pipiens 70 Chlorohydra sp. 0.22 vertebrate Rana chensinensis 161.9 Rana amurensis 0.071 Poecilia reticulate 135 Gobiocypris rarus 133 Xiphophorus helleri 127.29 Carassius auratus 119.1 Misgurnus bipartius 79.697 Pelteobagrus fulvidraco 76.22 Lepomis macrochirus 43 Oncorhynchus mykiss 24.23 Cyprinus carpio 1.907 Oryzias latipes 1.8

Table S10 LC50/EC50 for species in America for nitrobenzene Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrate Daphnia magna 24.49 Daphnia carinata 39.8 Ceriodaphnia dubia 54.4 Limnodrilus hoffmeisteri 116.12 Lymnaea stagnalis 64.5

10 Culex pipiens 70 vertebrate Carassius auratus 119.1 Danio rerio 101.73 Lepomis macrochirus 43 Oncorhynchus mykiss 0.000197 Cyprinus carpio 1.907 Pimephales promelas 99.15 Poecilia reticulata 135.00

Table S11 LC50/EC50 for species in China for 2,4,6-trichlorophenol Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrates Daphnia magna 1.73 Ceriodaphnia dubia 4.0 Daphnia carinata 7.5 Daphnia pulex 3.9 Limnodrilus hoffmeisteri 7.52 Radix plicatula 2.95 Chironomus sp. 2.06 Brachionus calyciflorus 3.0 vertebrates Rana nigromaculata 7.46 Bufo bufo gargarizans 8.63 Ctenopharyngodon idellus 3.54 Carassius auratus 4.31 Tilapia mossambica 5.66 Lepomis macrochirus 0.36 Oncorhynchus mykiss 1.21 Oryzias latipes 2.3

Table S12 LC50/EC50 for species in America for 2,4,6-trichlorophenol Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrates Daphnia magna 1.72 Ceriodaphnia dubia 4.0 Daphnia pulex 3.9 Limnodrilus hoffmeisteri 7.52 Aplexa hypnorum 5.5 Chironomus sp. 2.06 vertebrates Xenopus laevis 1.2 Carassius auratus 4.31

11 Tilapia mossambica 5.66 Danio rerio 0.96 Jordanella floridae 2.23 Lepomis macrochirus 0.36 Oncorhynchus mykiss 1.21 Pimephales promelas 5.32 Poecilia reticulata 0.009 Tanytarsus dissimilis 23.40

Table S13 LC50/EC50 for species in China for 2,4-dichlorophenol Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrates Daphnia magna 2.12 Moina macrocopa 15.53 Gammarus pulex 1.82 Chironomus sp. 2.74 Limnodrilus hoffmeisteri 9.89 Radix plicatula 3.37 vertebrates Bufo bufo gargarizans 9.46 Rana nigromaculata 9.85 Rana chensinensis 11.48 Carassius auratus 7.94 Tilapia mossambica 8.35 Lepomis macrochirus 1.6 Ctenopharyngodon idellus 5.25 Oncorhynchus mykiss 2.615 Oryzias latipes 4.5 Cyprinus carpio 13.9 Misgurnus anguillicaudatus 3.59

Table S14 LC50/EC50 for species in America for 2,4-dichlorophenol Taxa Species scientific name LC50/EC50 (mg L -1) Invertebrates Moina macrocopa 15.53 Daphnia magna 2.464 Gammarus pulex 1.82 Limnodrilus hoffmeisteri 9.89 Chironomus sp. 2.74 vertebrates Carassius auratus 7.94

12 Lepomis macrochirus 1.6 Ictalurus punctatus 1.773 Oncorhynchus mykiss 2.615 Pimephales promelas 8.614 Poecilia reticulata 5.5 Cyprinus carpio 13.9 Tilapia mossambica 8.35

Table S15 LC50/EC50 for species in China for parathion Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrates Daphnia magna 1.57 Daphnia pulex 0.58 Simocephalus serrulatus 0.28 Ceriodaphnia dubia 0.23 Artemia sinica 520 Procambarus sp. 250 Asellus aquaticus 13.71 Gammarus lacustris 3.5 Chironomus plumosus 39 Chironomus riparius 1.697 Cloeon dipterum 2.23 Hexagenia bilineata 15 Cheumatopsyche sp. 7.25 Tubifex sp. 5230 Limnodrilus sp. 5230 Cipangopaludina malleata 12000 Semisulcospira libertina 10000 Indoplanorbis exustus 12000 vertebrates Erpobdella octoculata 160 Pseudacris triseriata 1080 Rana catesbeiana 1320 Rana limnocharis 4744 Pseudorasbora parva 1780 Xiphophorus maculatus 55 Ctenopharyngodon idellus 1380

13 Cyprinus carpio 1804.94 Carassius auratus 2223 Lepomis macrochirus 158.24 Micropterus salmoides 592.55 Notopterus notopterus 580.76 Oncorhynchus mykiss 773.10 Oreochromis mossambicus 1759.55

Table S16 LC50/EC50 for species in America for parathion Taxa Species scientific name LC50/EC50 (μg L -1) Invertebrates Daphnia magna 1.0 Daphnia pulex 0.6 Simocephalus serrulatus 0.47 Orconectes nais 0.04 Asellus brevicaudus 1130 Palaemonetes kadiakensis 2.739 Procambarus sp. 250 Gammarus fasciatus 0.3628 Gammarus lacustris 3.5 Hexagenia bilineata 15 Pteronarcys californica 5.4 Pteronarcella badia 4.2 Lestes congener 3.0 Acroneuria pacifica 2.9 Claassenia sabulosa 1.5 Ischnura verticalis 0.64 Cloeon dipterum 2.227 Chironomus tentans 31 Chironomus riparius 1.697 Chaoborus sp. 0.8944 Peltodytes spp. 7 Tubifex sp. 5230 Limnodrilus sp. 5230 vertebrates Pseudacris triseriata 1000 Ictalurus punctatus 2650 Carassius auratus 2223 Salvelinus fontinalis 1760 Salvelinus namaycush 1920 Salmo trutta 1510 Oncorhynchus clarki 1560 Oncorhynchus gairdneri 1415 Pimephales promelas 839.6 Lepomis cyanellus 930

14 Lepomis macrochirus 510 Micropterus salmoides 620 Gambusia affinis 320 Poecilia reticulate 56 Oncorhynchus mykiss 773.10

15