1 SUPPLEMENTARY TABLE 1 FOR THE MS:

2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages.

3

4 Journal: Behavior Genetics

5

6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders

7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1

8

9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade

10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark.

11 2 Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology

12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg,

13 Denmark.

14 3 Aalborg Zoo, Aalborg, Denmark.

15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich,

16 Winterthurerstrasse 190, 8057 Zurich, Switzerland.

17

18 *Corresponding author:

19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University,

20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E-

21 mail [email protected].

22

1 1 Supplementary Table I: LA (± SD) at 3, 10 and 17 days of age, under dark and light regimes.

2

AGE 3 AGE 10 AGE 17 LINE DARK LIGHT DARK LIGHT DARK LIGHT A 570±322 534±231 918±531 650±362 944±664 663±370 B 622±510 581±187 862±506 571±157 660±515 545±276 C 745±321 619±364 745±321 590±351 737±342 563±320 5 786±413 364±151 549±400 317±139 634±271 601±389 6 695±203 335±134 716±236 347±150 587±219 219±61 7 415±251 272±124 450±191 296±184 500±303 296±119 9 1118±417 679±298 807±359 392±297 831±391 455±355 10 965±599 764±532 946±404 647±399 376±254 363±210 11 246±200 360±234 357±309 331±270 463±319 411±229 3

4 5

2 1 SUPPLEMENTARY FIGURE 1,2,3 FOR THE MS:

2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages.

3

4 Journal: Behavior Genetics

5

6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders

7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1

8

9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade

10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark.

11 2 Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology

12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg,

13 Denmark.

14 3 Aalborg Zoo, Aalborg, Denmark.

15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich,

16 Winterthurerstrasse 190, 8057 Zurich, Switzerland.

17

18 *Corresponding author:

19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University,

20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E-

21 mail [email protected].

22

23

24

3 1 Supplementary Figure 1: Daily locomotor activity (LA) at 3-day old age and during dark (Dark)

2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the

3 other two columns. The error bars show the standard errors.

20 20 20 Line A Line 5 Line 6

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 4 Dark Light Dark Light Dark Light

5

4 1 Supplementary Figure 2: Daily locomotor activity (LA) at 10-day old age and during dark (Dark)

2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the

3 other two columns. The error bars show the standard errors.

20 20 20 Line A Line 5 Line 6

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 4 Dark Light Dark Light Dark Light

5

5 1 Supplementary Figure 3: Daily locomotor activity (LA) at 17-day old age and during dark (Dark)

2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the

3 other two columns. The error bars show the standard errors.

4

20 20 20 Line A Line 5 Line 6

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11

15 15 15

A 10 A 10 A 10 L L L

5 5 5

0 0 0 5 Dark Light Dark Light Dark Light

6 7

6