<p> 1 SUPPLEMENTARY TABLE 1 FOR THE MS:</p><p>2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages.</p><p>3</p><p>4 Journal: Behavior Genetics </p><p>5</p><p>6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders </p><p>7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1</p><p>8</p><p>9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade </p><p>10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark.</p><p>11 2 Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology</p><p>12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg,</p><p>13 Denmark.</p><p>14 3 Aalborg Zoo, Aalborg, Denmark.</p><p>15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, </p><p>16 Winterthurerstrasse 190, 8057 Zurich, Switzerland.</p><p>17</p><p>18 *Corresponding author: </p><p>19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University,</p><p>20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E-</p><p>21 mail [email protected].</p><p>22</p><p>1 1 Supplementary Table I: LA (± SD) at 3, 10 and 17 days of age, under dark and light regimes.</p><p>2</p><p>AGE 3 AGE 10 AGE 17 LINE DARK LIGHT DARK LIGHT DARK LIGHT A 570±322 534±231 918±531 650±362 944±664 663±370 B 622±510 581±187 862±506 571±157 660±515 545±276 C 745±321 619±364 745±321 590±351 737±342 563±320 5 786±413 364±151 549±400 317±139 634±271 601±389 6 695±203 335±134 716±236 347±150 587±219 219±61 7 415±251 272±124 450±191 296±184 500±303 296±119 9 1118±417 679±298 807±359 392±297 831±391 455±355 10 965±599 764±532 946±404 647±399 376±254 363±210 11 246±200 360±234 357±309 331±270 463±319 411±229 3</p><p>4 5</p><p>2 1 SUPPLEMENTARY FIGURE 1,2,3 FOR THE MS:</p><p>2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages.</p><p>3</p><p>4 Journal: Behavior Genetics </p><p>5</p><p>6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders </p><p>7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1</p><p>8</p><p>9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade </p><p>10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark.</p><p>11 2 Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology</p><p>12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg,</p><p>13 Denmark.</p><p>14 3 Aalborg Zoo, Aalborg, Denmark.</p><p>15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, </p><p>16 Winterthurerstrasse 190, 8057 Zurich, Switzerland.</p><p>17</p><p>18 *Corresponding author: </p><p>19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University,</p><p>20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E-</p><p>21 mail [email protected].</p><p>22</p><p>23</p><p>24</p><p>3 1 Supplementary Figure 1: Daily locomotor activity (LA) at 3-day old age and during dark (Dark) </p><p>2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the </p><p>3 other two columns. The error bars show the standard errors.</p><p>20 20 20 Line A Line 5 Line 6</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 4 Dark Light Dark Light Dark Light</p><p>5</p><p>4 1 Supplementary Figure 2: Daily locomotor activity (LA) at 10-day old age and during dark (Dark) </p><p>2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the </p><p>3 other two columns. The error bars show the standard errors.</p><p>20 20 20 Line A Line 5 Line 6</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 4 Dark Light Dark Light Dark Light</p><p>5</p><p>5 1 Supplementary Figure 3: Daily locomotor activity (LA) at 17-day old age and during dark (Dark) </p><p>2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the </p><p>3 other two columns. The error bars show the standard errors.</p><p>4</p><p>20 20 20 Line A Line 5 Line 6</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line B Line 7 Line 9</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 Dark Light Dark Light Dark Light 20 20 20 Line C Line 10 Line 11</p><p>15 15 15</p><p>A 10 A 10 A 10 L L L</p><p>5 5 5</p><p>0 0 0 5 Dark Light Dark Light Dark Light</p><p>6 7</p><p>6</p>