OIE Reference Laboratory Reports s8

OIE Reference Laboratory Reports Activities in 2010

Name of disease (or topic) for Highly pathogenic avian influenza which you are a designated OIE Reference Laboratory:

Address of laboratory: Laboratory of Microbiology, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, 060-0818, Japan

Tel.: (+81-11) 706.52.07

Fax: (+81-11) 706.52.73

e-mail address: [email protected]

website: http://www.vetmed.hokudai.ac.jp/

Name of Head of Laboratory Prof. Hiroshi Kida (Responsible Official):

Name of OIE Reference Expert: Prof. Hiroshi Kida

Name of writer of this report (if different from above):

Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 1 Higly pathogenic avian influenza

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Test For Specificity Total

Embryonated chicken eggs Virus isolation Group 2,515

RT-PCR Gene detection Group and Type 2,000

HI Antibody Type 100

NI Antibody Type 100

2. Production and distribution of diagnostic reagents

Chicken anti-sera against H1 -H16 reference strains were produced in our laboratory for diagnosis purpose.

Part II: Activities specifically related to the mandate of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

To establish a global reference standard serum for H5 influenza A virus, through a collaborative exercise among OFFLU partners antisera against H5 influenza viruses were listed and evaluated in OIE reference laboratories including our laboratory (Management of the project: Veterinary Laboratories Agency, UK) since 2009.

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

H5 avian influenza viruese were supplied to NIAID, NIH, USA.

Equine influenza virus was supplied to University of Iowa, USA.

5. Research and development of new procedures for diagnosis and control

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) was evaluated as a screening method for the detection of influenza viruses in the fecal materials of water birds (Yoshida et al., 2011, J Vet Med Sci, in press).

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

Influenza virus isolates of 68 combinations of HA and NA subtypes have been isolated from fecal samples of ducks in surveillance studies. So far, 76 other combinations have been generated by the genetic reassortment procedure in chicken embryos. Thus, non-pathogenic avian influenza viruses of all 144 combinations of HA and NA subtypes have been stocked. The aim of the database (http://virusdb.czc.hokudai.ac.jp/index.html) is to share the data and biological materials for research and development of vaccines and diagnostic use for the control of animal and human influenza.

2 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 Higly pathogenic avian influenza

7. Provision of consultant expertise to OIE or to OIE Members

Hiroshi Kida: Second Global Conference of OIE Reference Laboratories and Collaborating Centers. Paris, France, 21 June, 2010.

Hiroshi Kida: OFFLU Annual Technical Meeting. Roma, Italy, 16 November, 2010.

Yoshihiro Sakoda: Third OIE Regional Expert Group Meeting for Implementation of the Programme on Surveillance of Wild Birds and Domestic Animals along Migratory Flyways. Tokyo, Japan, 16-17 June, 2010.

Yoshihiro Sakoda: Regional Meeting on Public Health Research Agenda for Influenza in South-East Asia. SEARO, New Delhi, India, 18-20 August, 2010,

8. Provision of scientific and technical training to personnel from other OIE Members

In November 12, 2010, training course for “Veterinary Technology for Farm Animals” was opened at Hokkaido University, Sapporo, Japan. Trainees from 6 Asian and African countries were invited for sharing latest information and learn the fundamental diagnosis for avian influenza (Virus isolation, HI test, NI test, IVPI method).

9. Provision of diagnostic testing facilities to other OIE Members

Virus isolation, HA/NA subtyping and phylogenetic analysis were requested from Mongolia for dead wild birds. Identified highly pathogenic avian influenza viruses were deposited in our virus library and sequence data were registered in public database. All information was reported to Mongolian government immediately.

10. Organisation of international scientific meetings on behalf of OIE or other international bodies

No activity.

11. Participation in international scientific collaborative studies

No activity.

12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

 Presentations at international conferences and meetings

Hiroshi Kida: Invited Lecture as a Visiting Professor for 2010 at All India Institute of Medical Sciences; “For the control of avian, swine, and pandemic influenza”. New Delhi, India, 20 March 2010.

Hiroshi Kida:“Avian, Swine, and Pandemic Influenza-Importance of the Control of Avian and Seasonal Influenza”. BIT’s 1st World Congress of Virus and Infections Busan, South Korea, 31 July 2010.

 Scientific publications in peer-reviewed journals

1) Cheng, M. C., Soda, K., Lee, M. S., Lee, S. H., Sakoda, Y., Kida, H., and Wang, C. H. 2010. Isolation and characterization of potentially pathogenic H5N2 influenza virus from a chicken in Taiwan in 2008. Avian Diseases, 54: 885-893.

2) Feng, F., Miura, N., Isoda, N., Sakoda, Y., Okamatsu, M., Kida, H., and Nishimura, S. 2010. Novel trivalent anti-influenza reagent. Bioorganic & Medicinal Chemistry Letters, 20: 3772-3776.

Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 3 Higly pathogenic avian influenza

3) Igarashi, M., Ito, K., Yoshida, R., Tomabechi, D., Kida, H., and Takada, A. 2010. Predicting the antigenic structure of the pandemic (H1N1) 2009 influenza virus hemagglutinin. PLoS One, 5: e8553.

4) Itoh, Y., Ozaki, H., Ishigaki, H., Sakoda, Y., Nagata, T., Soda, K., Isoda, N., Miyake, T., Ishida, H., Okamoto, K., Nakayama, M., Tsuchiya, H., Torii, R., Kida, H., and Ogasawara, K. 2010. Subcutaneous inoculation of a whole virus particle vaccine prepared from a non-pathogenic virus library induces protective immunity against H7N7 highly pathogenic avian influenza virus in cynomolgus macaques. Vaccine, 28: 780-789.

5) Iwai, A., Shiozaki, T., Kawai, T., Akira, S., Kawaoka, Y., Takada, A., Kida, H., and Miyazaki, T. 2010. Influenza A virus polymerase inhibits type I interferon induction by binding to interferon {beta} promoter stimulator 1. Journal of Biological Chemistry. 285, 32064-32074.

6) Miyake, T., Soda, K., Itoh, Y., Sakoda, Y., Ishigaki, H., Nagata, T., Ishida, H., Nakayama, M., Ozaki, H., Tsuchiya, H., Torii, R., Kida, H., and Ogasawara, K. 2010. Amelioration of pneumonia with Streptococcus pneumoniae infection by inoculation with a vaccine against highly pathogenic avian influenza virus in a non- human primate mixed infection model. J Med Primatol, 39: 58-70.

7) Okamatsu, M., Tanaka, T., Yamamoto, N., Sakoda, Y., Sasaki, T., Tsuda, Y., Isoda, N., Kokumai, N., Takada, A., Umemura, T., and Kida, H. 2010. Antigenic, genetic, and pathogenic characterization of H5N1 highly pathogenic avian influenza viruses isolated from dead whooper swans (Cygnus cygnus) found in northern Japan in 2008. Virus Genes, 41: 351-357.

8) Sakoda, Y., Sugar, S., Batchluun, D., Erdene-Ochir, T. O., Okamatsu, M., Isoda, N., Soda, K., Takakuwa, H., Tsuda, Y., Yamamoto, N., Kishida, N., Matsuno, K., Nakayama, E., Kajihara, M., Yokoyama, A., Takada, A., Sodnomdarjaa, R., and Kida, H. 2010. Characterization of H5N1 highly pathogenic avian influenza virus strains isolated from migratory waterfowl in Mongolia on the way back from the southern Asia to their northern territory. Virology, 406: 88-94.

9) Sasaki, T., Kokumai, N., Ohgitani, T., Imamura, T., Sawata, A., Lin, Z., Sakoda, Y., and Kida, H. 2010. A comparison of the antibody responses between specific pathogen-free and commercial layers immunized with an influenza vaccine prepared from inactivated non-pathogenic H5N1 virus by single shot. Journal of Veterinary Medical Science, 72: 819-821.

10) Takeyama, N., Minari, K., Kajihara, M., Isoda, N., Sakamoto, R., Sasaki, T., Kokumai, N., Takikawa, N., Shiraishi, R., Mase, M., Hagiwara, J., Kodama, T., Imamura, T., Sakaguchi, M., Ohgitani, T., Sawata, A., Okamatsu, M., Muramatsu, M., Tsukamoto, K., Lin, Z., Tuchiya, K., Sakoda, Y., and Kida, H. 2010. Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1). Veterinary Microbiology, 147: 283-291.

11) Tanaka, T., Sunden, Y., Sakoda, Y., Kida, H., Ochiai, K., and Umemura, T. 2010. Lipopolysaccharide treatment and inoculation of influenza A virus results in influenza virus-associated encephalopathy-like changes in neonatal mice. Journal of Neurovirology, 16: 125-132.

13. Inscription of diagnostic kits on the OIE Register

i) Did you participate in expert panels for the validation of candidate kits for inscription on the OIE Register? If yes, for which kits? No

ii) Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for which kits? No

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4 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010