Isolation and Characterization of a R2R3-MYB Transcription Factor Gene Related to Anthocyanin
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Isolation and characterization of a R2R3-MYB transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.)
Chonghui Li1,3 • Jian Qiu2 • Guangsui Yang1,3 • Surong Huang1,3 • Junmei Yin1,3 CH. Li and J. Qiu contributed equally to this work Corresponding author * Junmei Yin Address: Tropical Crops Genetic Resources Institute, the Chinese Academy of Tropical Agricultural Sciences (CATAS) / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou 571737, China e-mail: [email protected]
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Online Resource 2 Anthocyanin) levels in extracts from leaves of anthurium cultivar ‘Tropical’. a The total anthocyanin contents in the young and mathure leaves. The data were presented as the mean ± SD (n=3). b The anthocyanins are evident by visual inspection of the extracts after chlorophyll extraction by chloroform.
M L1 L2 L3 L4 L5 L6 L7 L8 L9 L10 1200 bp 800 bp 500 bp 200 bp Online Resource 3 Gel electrophoresis confirmation of genomic PCR products of AaMYB2 in transgenic tobacco lines. M: marker, L: transgenic lines Online Resource 4 Pearson’s correlation (r) of transcript levels of AaMYB2 with anthocyanin biosynthetic genes as well as correlations of total anthocyanin content with responsible genes in the developing red spathes of cultivars ‘Tropical’ and ‘Vitara’ Genes AaMYB2 Total anthocyanin content r p value r p value AaCHS 0.487** 0.006 –0.174 0.359 AaF3H 0.510** 0.004 –0.122 0.522 AaF3'H 0.339 0.067 0.298 0.110 AaDFR 0.248 0.186 –0.450* 0.013 AaANS 0.461* 0.010 –0.262 0.162 AaMYB 0.458* 0.011 2 * Significant at p = 0.05, ** significant at p = 0.01
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S1 S2 S3 S4 S5 Online Resource 5 Detection of AaMYB2 and anthocyanin biosynthetic genes expression levels in the developmental leaves of anthurium cultivar ‘Tropical’. a Expression levels of AaMYB2 and anthocyanin biosynthetic genes. The normalized expression of all genes at stage 1 were served as the calibrator for the calculation of relative expression levels in all stages (relative expression at stage 1 arbitrarily set to one). The data were presented as the mean ± SD (n=3). b The developmental stages of leaves.