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AccuLean Portable Ground Meat Analyzer

User’s Manual Version 4.0

This manual provides you the information needed to operate the AccuLean Portable Ground Meat Analyzer.

ZELTEX, INC. 130 Western Maryland Parkway Hagerstown, Maryland 21740 USA

Phone: 301-791-7080 Fax: 301-733-9398 E-mail: [email protected] © Zeltex, Inc. 2007

Table of Contents Table of Contents...... 2 WARNING...... 3 Introduction...... 4 Appearance and Part Names...... 4 Keypad Description...... 5 Other Parts and Accessories...... 5 Power...... 5 Printer Paper Installation for Optional Printer...... 6 Using the Clamshell Cup...... 6 Using the AccuLean...... 7 Turning on the AccuLean for the First Time...... 7 Measuring a Sample...... 7 CM / As-Is...... 8 Changing Products...... 9 Adjusting the Calibration...... 9 Adjusting the Time and Date...... 10 Reset Automatic ID Number...... 11 Adjusting Number of Read Cycles...... 11 Connecting to a Computer...... 11 Storing Samples and Results...... 12 Enabling/Disabling the Optional Printer...... 12 Paper Feed...... 13 Warning and Error Messages...... 14 General Specifications...... 16 Glossary...... 17 Appendix 1: Analyzed Sample Form...... 18 Appendix 2: Combination Lock for Carrying Case...... 19

WARNING

THE ACCULEAN PORTABLE GROUND MEAT ANALYZERS ARE NOT EXPLOSION-PROOF:

REASONABLE CARE MUST BE USED IN HANDLING THESE INSTRUMENTS.

Introduction

Appearance and Part Names

Clamshell Cup with inserts

Keypad Description Key Name Description

MEASURE This key is used to make measurements.

ENTER This key is used after a selection has been made on the menu.

SELECT This key is used before entering any adjustable programming ESC within the instrument. It can also be used to cancel a current entry in the instrument.

PRODUCT This key is used to make a product selection.

CM* This key toggles the displayed result between a Constant AS-IS Moisture basis or on an AS-IS basis.

▲ ▼ These arrow keys cycle through menu selections.

Other Parts and Accessories The AccuLean is shipped with the following parts and accessories. Contact Zeltex if you would like to purchase additional accessories.

 AccuLean  Calibration Software CD  2 Clamshell Cups  Quick Start Manual  Sample bags  User’s Manual  A/C Adapter  Calibration Manual  RS232 Cable  Calibration Numbering Setup  6 Batteries Manual  Carrying Case

Optional:  Printer  Printer Paper

Power The AccuLean can be powered by batteries or an A/C Adapter.

1) Battery Installation: Insert six 1.5V “AA” size alkaline batteries into the battery compartment located on the lower back of the main body. Be sure that the batteries are oriented as indicated in the battery compartment.

Note: When you are running the AccuLean on batteries, please make sure that the instrument is shut off when not in use. The batteries will last much longer if the instrument is turned off when sitting idle.

2) A/C Adapter: Connect the A/C adapter cable to the A/C adapter jack located on the right hand side of the instrument (the jack closest to the top of the instrument).

Printer Paper Installation for Optional Printer 1) Remove the clear plastic cover from the printer.

2) Cut the end of the roll of paper at an angle to form a point in the center.

3) Feed the paper into the slit inside the Paper Roll Compartment. Use the Paper Feed option to advance the paper (see Paper Feed).

Using the Clamshell Cup The A0636 Clamshell Cup allows the analysis of pastes and slurries in clear “whirl-pak”–type bags. The Clamshell Cup includes an internal A0633 Thermistor Module (a), contact strips (b), windows in the body and door (c), and an assortment of different thickness windows (d).

The Clamshell Cups that have been shipped with your instrument have been setup to work with your specific product. If you wish to create your own calibration then you may need to use different windows with your Clamshell Cup (see the AccuLean Calibration Software Manual.)

Using the AccuLean

A NOTE ON THE TEXT

Throughout this manual, keystrokes are represented in bold type; references to messages on the computer screen or the AccuLean display screen are in “quotes.”

Turning on the AccuLean for the First Time It is recommended that you measure ten samples of the product that the instrument has been pre-calibrated for. (These samples must be pre-analyzed by your lab.) Write the analysis in the Analyzed Sample Form provided in Appendix 1 and then use the Statistical Analysis portion of the provided Calibration Software to determine if an adjustment is necessary. If you are unable to use the Calibration Software or are simply uncomfortable with determining when an adjustment is necessary, feel free fax the Analyzed Sample to Zeltex or email the predicted values to Zeltex for assistance:

FAX: 1-301-733-9398

PHONE in the USA: 1-800-732-1950 outside the USA: 1- 301-791-7080

EMAIL: [email protected]

Zeltex will give you any necessary adjustments.

Measuring a Sample 1) Turn the AccuLean on. Once the test sequence is complete, “Measure Standard” will be displayed.

2) Select the product according to the sample type to be measured. (See Changing Products)

3) Fill the sample bag and insert it into the Clamshell Cup.

4) Make sure there is nothing in the sample chamber and close the lid over the sample chamber.

5) Press MEASURE. The display will show “Reading . . .,” then “Insert Sample.” This indicates that the instrument has been standardized. The instrument must be standardized prior to making any measurement.

6) Lift the lid from the sample chamber and place the cup containing the sample in the chamber.

7) Replace the lid over the sample in the chamber. This shield must always be used when measuring a sample or a standard; otherwise, the results will be incorrect.

8) Press MEASURE. The display will show “Reading . . .,” then “Remove & Turn.” Remove the Clamshell Cup, turn and replace the bag. Do not turn the Clamshell Cup. Insert the cup and close the lid.

9) Press MEASURE. The display will show “Reading . . .,” then “Remove & Reload.” Remove the sample cup, turn the bag once more, and replace the sample cup in the sample chamber and close the lid.

10) Press MEASURE. The display will show “Reading . . .,” then “Remove & Turn.” Remove the Clamshell Cup, turn and replace the bag. Do not turn the Clamshell Cup. Insert the cup and close the lid.

11) Press MEASURE. The display will show “Reading . . .,” then “Remove & Press M.” Remove the sample.

12) Close the lid over the empty chamber and press MEASURE.

13) The results will be displayed. Use the down arrow key to list the results of each constituent.

14) To measure another sample of the same product, press SELECT ESC to clear the results. The instrument will return to “Measure Standard.” Repeat steps 3-14.

At any prompt where you have to either enter a sample or remove a sample and press MEASURE, the instrument will allow you only 50 seconds to do so. If after the elapse of 50 seconds you have not completed the task required, the AccuLean will flash “Time Out!” and automatically return to “Measure Standard.” This feature is designed to prevent readings from becoming “stale.”

Note: The AccuLean has a number of warnings for possible bad readings. See Warning and Error Messages in this manual for details.

CM / As-Is When displaying measurement results, the instrument displays the Constant Moisture (CM) calculation first by default. Pressing the CM/AS-IS Button will toggle back and forth between CM and AS-IS.

If you should wish to display the AS-IS results first, simply press the CM/AS-IS button while the display says "Measure Standard". When pressed, the display will say "AS-IS DEFAULT". Any readings taken afterwards will show the AS-IS results first. To view the CM results first again, press the CM/AS-IS button again while the display says "Measure Standard" and the display will say "CM DEFAULT".

Changing Products 1) Press PRODUCT.

2) Press the arrow keys (▲▼) to cycle through the products. The different products will be listed. (The AccuLean is able to store ten products, numbered as 0-9.)

3) Press ENTER when the product to be tested is on the display. Only one product can be selected at a time.

Adjusting the Calibration The AccuLean has been carefully calibrated at the factory or at your location; however, sometimes it is necessary to adjust the calibration for a slope and/or bias.

1) Bias Adjustment (shifts all readings up or down the same amount)

2) Slope Adjustment (changes the angle of prediction line to agree with the lab)

Before making any adjustments, you must first measure at least 5 samples for which you have laboratory analysis. These samples should also cover the range of the calibration. Once the samples have been measured, use the Statistical Analysis portion of the provided Calibration Software to determine if an adjustment is necessary. Print the Statistical Analysis and note the adjustment that the software recommends. If you are unable to use the Calibration Software or are simply uncomfortable with determining when an adjustment is necessary, feel free to use the Analyzed Sample Form found in Appendix 1 of this manual and contact Zeltex for assistance.

Once you have determined the adjustment to be made, follow these instructions for making an adjustment to the AccuLean:

1) Turn on the instrument.

2) Make sure you are in the product that requires the bias adjustment. (See Changing Products)

3) When “Measure Standard” is displayed, press SELECT ESC.

4) Use the arrow keys to cycle through the options until “Slope and Bias” is displayed. Press ENTER.

5) The product and constituent appear: (Example: PØ WHT C1 PRO)

6) Use the arrow keys to cycle through the constituents. Press ENTER after the constituent for which you want to adjust the slope and/or bias appears.

7) “Slope 1.ØØ will appear. Use the arrow keys to cycle through the numbers. (If you do not wish to make a slope adjustment, leave the slope adjustment set to 1.ØØ). Press ENTER when the correct slope adjustment is displayed. The adjusted slope will now be displayed, press ENTER to confirm.

8) "Bias Ø.Ø" will appear. Use the arrow keys to cycle through the numbers. (If you do not wish to make a bias adjustment, leave the bias adjustment set to Ø.Ø). Press ENTER when the correct bias is displayed. The adjusted bias will now be displayed. Press ENTER to confirm. “COMPLETE” will appear.

If for any reason you must abort, press SELECT ESC to abort the adjustment.

Adjusting the Time and Date Adjusting the Time:

2) At the “Measure Standard” prompt, press SELECT ESC.

3) Use the arrow keys to cycle through the menu until “Set Time” appears.

4) Press ENTER.  The up arrow key (▲) cycles through the hours.  The down arrow key (▼) cycles through the minutes.  The CM* AS-IS key zeroes the seconds.

5) Press ENTER after the time has been adjusted.

Adjusting the Date:

1) Turn instrument on.

2) At the “Measure Standard” prompt, Press SELECT ESC.

3) Use the arrow keys to cycle through the menu until “Set Time” appears.

4) Press ENTER.  The up arrow key (▲) cycles through the months.  The down arrow key (▼) cycles through the days.  The CM* AS-IS key zeroes the years.

5) Press ENTER after the date has been adjusted.

Reset Automatic ID Number This selection is used to reset the automatic sampling number to #1.

2) At the “Measure Standard” prompt, Press SELECT ESC.

3) Use the arrow keys to cycle through the menu until “Reset ID #” appears.

4) Press ENTER. “ID # Reset” will be displayed. The next sample run will be labeled sample #1.

Adjusting Number of Read Cycles The AccuLean is designed to allow you to use between 2 and 9 repetitions to get the maximum accuracy with the minimum time needed for your samples. Each product can be set to read a different number of times. Zeltex recommends using four (4) repetitions.

3) Use the arrow keys to cycle through the menu to find “Set Readings.” Press ENTER.

4) The number of readings is displayed. Use the arrow keys to cycle through the numbers. Press ENTER at the number of your choice.

Connecting to a Computer The AccuLean comes with Calibration Software that allows you to create your own calibrations, make backups of the current calibration, and view the optical data for a sample. To do this you must connect the AccuLean to the computer using the supplied RS-232 Cable and put the AccuLean into Data Link mode.

1) Turn off the instrument.

2) Connect the RS-232 cable to the RS-232 Port on the side of the AccuLean.

3) Connect the RS-232 Cable to a free RS-232 port on the computer.

6) Use the arrow keys to cycle through the menu until “Data Link” appears.

7) Press ENTER. “DATA LINK” will be displayed in capital letters.

Storing Samples and Results The AccuLean has the ability to store the OD’s and the calculated results for 25 samples in battery-backed RAM. These are stored in the same memory as the calibration constants. This means that the samples are preserved even when the AccuLean is turned off. The samples and results that are saved in the AccuLean's memory can be downloaded to the PC using the Calibration Software.

To save the last sample that was read:

1) Press SELECT ESC.

2) Use the arrow keys to cycle through the menu until “Store Samples” appears. Press ENTER.

3) “Saved #______“ indicates the number (1-25) of the saved sample in the AccuLean’s memory.

After saving 25 samples, the instrument will wrap around and begin to save again in position 1. This will overwrite the previous sample(s).

To save the results from the last reading:

1) Press SELECT ESC.

2) Use the arrow keys to cycle through the menu until “Store Results” appears. Press ENTER.

3) “Saved #______“ indicates the number (1-25) of the saved results in the AccuLean’s memory.

After saving 25 results, the instrument will wrap around and begin to save again in position 1. This will overwrite the previous sample(s).

Enabling/Disabling the Optional Printer Enabling the printer:

3) Use the arrow keys to cycle through the menu until “Printing” appears. Press ENTER.

4) “PRN ENABLED” will be displayed. Press ENTER.

NOTE: Each time the AccuLean is turned on, the printer must be enabled.

Disabling the printer:

3) Use the arrow keys to cycle through the menu until “Printing” appears. Press ENTER.

4) “PRN DISABLED” will be displayed. Press ENTER.

Paper Feed 1) Turn on the instrument.

3) Use the arrow keys to cycle through the menu until “Paper Feed” appears.

4) Press ENTER. The paper will advance one line each time ENTER is pressed.

5) Press SELECT ESC to return to “Measure Standard.”

Warning and Error Messages The AccuLean has a number of warnings and error codes built into it. Some of them are overridden automatically on the analyzer. Others require you to start the reading again. If the instrument overrides the warning, it will still print the warning on the printout. When a warning is overridden, analyze the sample a second time to ensure an accurate reading. If you believe that you are getting one of the warning or error messages in error or you have any questions about these messages, please contact Zeltex for technical support.

Warning/Error Message Explanation

1) ERROR: TOO DARK This error occurs if the energy reaching the detector is less than the acceptable level. This is normally caused by the sample being too thick.

2) ERROR: TOO LITE This will be displayed if the detector is in saturation. This normally is caused by not placing the light shield on correctly during the reading or Zero Adjust or by the sample being too thin.

3) AMB. TEMP. HIGH The AccuLean was set up with a temperature check. If the instrument temperature is too cold or or too hot, these warnings will appear on the printout.

AMB. TEMP. LOW

4) SMP. TEMP. HIGH The AccuLean was set up with a temperature check. If the instrument temperature is too cold or or too hot, these warnings will appear on the printout.

SMP. TEMP. LOW

5) VALUE TOO HIGH This occurs when the reading for a particular constituent is outside the range of the calibration. or The results of samples read that lie outside of the calibration range are not as reliable. VALUE TOO LOW

6) BAD CURVE This will appear only if A, B, and C have been activated for the calibration. This can happen if you have the wrong product active.

7) HIGH VARIANCE This is displayed if the variance between the two readings is more than normal variance in the sample. This tells you that the repeatability within the sample is not as reasonable as we would expect. This could be caused by light leaks or just

variations in the samples.

8) TIMEOUT At any prompt where you have to either enter a sample or remove a sample and press ENTER, the instrument will allow you only 30 seconds to do so. If, after the elapse of 30 seconds, you have not completed the task required, the AccuLean will flash “TIMEOUT” and automatically return to “Zero Adjust”. This is a feature designed to not allow the readings to get stale.

9) LOW BATTERIES This is displayed when the batteries need to be replaced.

10) RAM RESET This message is displayed when there is a failure with the internal memory. This will cause all of the calibration constants to be lost. If this happens, you must restore a backup of the calibrations using the Calibration Software (refer to Creating/Restoring a Backup File in the AccuLean Calibration Software Manual).

If the message occurs more than once, please contact Zeltex for further instructions.

General Specifications

1. MEASUREMENT Measuring Principle: Near-Infrared Transmittance based on technology from the United States Department of Agriculture using 14 wavelengths covering the range from 893 nanometers to 1045 nanometers.

2. PRINTER OUTPUT (Optional) Measured Results with time, date, and identification

3. POWER SOURCE Six AA 1.5V alkaline batteries or 9V A/C adapter (included)

4. DIMENSIONS Main Body - 4.7 x 13.5 x 2.2 inches

5. WEIGHT 3 lbs.

Glossary

Accuracy – Refers to how closely the readings of the known samples match the samples’ predetermined lab values in a given instrument.

Bias – The average overall shift of the calculated results from the ideal laboratory values. A Bias Adjustment will compensate for this.

Constants (also referred to as K values) – Figures determined during calibration of the instrument which are used in the equation:

Result = K0 + (K1OD1 + K2OD2 + . . . KNODN)

Constituent – One of the properties of the product being measured, such as Protein or Moisture.

Data Link – The mode that the AccuLean must be in to communicate with the Calibration Software.

ID’s – The identification numbers of the samples.

Multiple Linear Regression (MLR) – The mathematical process to fit the optical data to the lab data.

Optical Data (OD’s) –The data obtained by the instrument from each of its filters which it then applies to the above equation.

Precision – Refers to how closely the same sample repeats its readings in a given instrument

Reduce / Reduction – See Multiple Linear Regression

Regression – See Multiple Linear Regression

Slope and Bias – More than just an average overall shift of the calculated results from the ideal laboratory values. A Slope and Bias adjustment will compensate for this.

Thermistor – The probe in the sample chamber that measures the sample temperature.

Appendix 1: Analyzed Sample Form

SEND TO: Zeltex, Inc. USA, FAX NO.: 1-301-733-9398

FROM: Company name, Country ______

CONTACT PERSON: (please print) ______

PHONE: Country code ______Number ______

FAX: ______

Enter in the Analyzed Sample columns, ten (10) samples that have been analyzed by your lab. In the corresponding columns, enter the AccuLean readings for the same samples. Fax to Zeltex for advice concerning any needed adjustments.

AccuLe AccuLe AccuLe AccuLe Samp. LAB LAB LAB LAB an an an an # CON 1 CON 2 CON 3 CON 4 CON 1 CON 2 CON 3 CON 4

Appendix 2: Combination Lock for Carrying Case

Your combination lock is set at the factory to open at 000. You may leave it at this setting or you can set your own secret combination in the following manner:

1) With the lock open, push button (1) in the direction of the arrow and hold down until the second operation has been performed.

2) Set number wheels (2) to the desired combination. Do not forget the combination you have set!

3) Now release the button (1). Press the button again as a check.

Your case will now open only at your secret combination. If you want to change the combination again, simply repeat these steps.